当前位置： SCI文献检索 > CYTOKINE期刊下所有文献 > An innovative three-dimensional model of normal human skin to study the proinflammatory psoriatic effects of tumor necrosis factor-alpha and interleukin-17.

An innovative three-dimensional model of normal human skin to study the proinflammatory psoriatic effects of tumor necrosis factor-alpha and interleukin-17.

Abstract：

BACKGROUND:Among all cytokines involved in the pathogenesis and in the progression of psoriasis, Tumor Necrosis Factor (TNF)-alpha and interleukin (IL)-17 play a pivotal role. OBJECTIVE:The aim of the present study was to mimic a psoriatic microenvironment and to investigate the early effects of TNF-alpha and IL-17 in a three-dimensional model of organotypic normal human skin. METHODS:Human skin explants were obtained from plastic aesthetic surgery of healthy young women 20-40years old (n=7). The study was approved by the Institutional Review Board and written informed consent was obtained from all subjects. Bioptic fragments were cultured at the air-liquid interface overnight in a Transwell system and further divided before adding either 50ng/ml IL-17 or 100ng/ml TNF-alpha or a combination of both cytokines. For each subject, a control sample was cultured without any cytokine. Samples were harvested 24 or 48h after cytokine incubation. At both time points and for all cytokine treatments a bioptic fragment obtained from each patient was processed. Epidermal proliferation, expressions of terminal differentiation (keratin 10, K10, and 14, K14) and of intercellular adhesion (occludin for tight junctions and E-cadherin for adherens junctions) biomarkers were investigated by indirect immunofluorescence. RESULTS:IL-17 and TNF-alpha induced an early and statistically significant inhibition of keratinocyte proliferation (more than 80% compared with their respective controls). At 24h, the combination of both cytokines did not further reduce cell proliferation. Starting from 24h of incubation, a non-continuous occludin expression in the granular layer was observed after both IL-17 and TNF-alpha exposure. Immunolabelling for E-cadherin in adherens junctions, for K10 in the suprabasal layers, and for K14 in the basal layer was similar in all experimental groups and unaffected after cytokine treatment. CONCLUSIONS:These results suggest that in this experimental model IL-17 and TNF-alpha induced an early alteration of the homeostasis of the inner proliferative layer and of the upper granular layer, as shown by cell proliferation inhibition and occludin expression.

journal_name

Cytokine

journal_title

Cytokine

authors

Donetti E,Cornaghi L,Gualerzi A,Baruffaldi Preis FW,Prignano F

doi

10.1016/j.cyto.2014.03.003

subject

Has Abstract

pub_date

2014-07-01 00:00:00

pages

1-8

issue

1

eissn

1043-4666

issn

1096-0023

pii

S1043-4666(14)00071-4

journal_volume

68

pub_type

杂志文章

相关文献

CYTOKINE文献大全
  • Evaluation of endothelial biomarkers as predictors of organ failures in septic shock patients.

    abstract：BACKGROUND:Endothelial injury is recognized to trigger organ failures during the first 48h of septic shock. We evaluate endothelial biomarkers at ICU admission in their ability to predict severity, outcome, and organ failures in septic shock patients. METHODS:This prospective observational pilot study was conducted in...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2015.02.013

    authors： Palud A,Parmentier-Decrucq E,Pastre J,De Freitas Caires N,Lassalle P,Mathieu D

    更新日期：2015-06-01 00:00:00

  • Low serum interleukin-6 levels as a predictive marker of recurrence in patients with hepatitis B virus related hepatocellular carcinoma who underwent curative treatment.

    abstract：BACKGROUND:We aimed to investigate the use of novel serum biomarkers for predicting the recurrence and survival of patients with hepatitis B virus (HBV)-related early hepatocellular carcinoma (HCC) after hepatic resection or radiofrequency ablation (RFA). METHODS:One hundred and five patients with HBV-related HCC, who...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2015.02.027

    authors： Cho HJ,Kim SS,Ahn SJ,Park SY,Park JH,Kim JK,Wang HJ,Cheong JY,Cho SW

    更新日期：2015-06-01 00:00:00

  • Serum levels of interleukin-2 predict the recurrence of atrial fibrillation after pulmonary vein ablation.

    abstract：AIMS:Interleukin-2 has a significant antitumor activity in some types of cancer, and has been associated with the development of atrial fibrillation (AF). In addition, IL-2 serum levels in recent onset AF have been related with pharmaceutical cardioversion outcomes. We evaluated the hypothesis that a relationship exist...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2015.01.026

    authors： Cabrera-Bueno F,Medina-Palomo C,Ruiz-Salas A,Flores A,Rodríguez-Losada N,Barrera A,Jiménez-Navarro M,Alzueta J

    更新日期：2015-05-01 00:00:00

  • Chemokines in pregnant women with sickle cell disease.

    abstract：:Pregnancy in sickle cell disease is a problem due to the adverse outcomes related to the disease. Research into the role of chemokines in sickle cell disease is available, but studies investigating the disease in pregnancy are scarce. Our data show the chemokine profiles of pregnant women with sickle cell disease comp...

    journal_title：Cytokine

    pub_type： 临床试验,杂志文章

    doi：10.1016/j.cyto.2018.07.002

    authors： Hazin-Costa MF,da Silva Aráujo A,Guerra GL,da Matta MC,Torres LC,Souza AI

    更新日期：2019-01-01 00:00:00

  • Association of TNF-α-(308(GG)), IL-10(-819(TT)), IL-10(-1082(GG)) and IL-1R1(+1970(CC)) genotypes with the susceptibility and progression of leprosy in North Indian population.

    abstract：:Leprosy is an infectious disease caused by M. leprae. We analyzed 48 cytokine polymorphisms in 13 (pro as well as anti-inflammatory) cytokine genes using PCR-SSP assay in 102 leprosy patients and 120 healthy controls with intent to find out a link between cytokine polymorphisms and disease susceptibility. TNF-α (-308)...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2015.01.014

    authors： Tarique M,Naqvi RA,Santosh KV,Kamal VK,Khanna N,Rao DN

    更新日期：2015-05-01 00:00:00

  • Comparison of RT-qPCR and Nanostring in the measurement of blood interferon response for the diagnosis of type I interferonopathies.

    abstract：:Type I interferonopathies are characterized by an increase of circulating type I interferon (IFN) concentration. Type I interferonopathies refer to rare Mendelian genetic disorders such as Aicardi-Goutières Syndrome (AGS) as well as more frequent and polygenic auto-immune diseases like systemic lupus erythematosus (SL...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2018.10.023

    authors： Pescarmona R,Belot A,Villard M,Besson L,Lopez J,Mosnier I,Mathieu AL,Lombard C,Garnier L,Frachette C,Walzer T,Viel S

    更新日期：2019-01-01 00:00:00

  • Prognostic value of visfatin in various human malignancies: A systematic review and meta-analysis.

    abstract：:Although numerous studies have shown that visfatin is linked to several cancers, its prognostic value is still unclear. This first comprehensive meta-analysis was performed to evaluate the prognostic effect of visfatin in cancer patients. A systematic search was conducted for relevant studies in health-related electro...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2019.154964

    authors： Mohammadi M,Moradi A,Farhadi J,Akbari A,Pourmandi S,Mehrad-Majd H

    更新日期：2020-03-01 00:00:00

  • TGF-beta1 gene polymorphisms influence the course of the disease in non-Hodgkin's lymphoma patients.

    abstract：:Non-Hodgkin's lymphomas (NHL) constitute a heterogenous group of mainly B-cell lymphoproliferative diseases with different patterns of clinical behaviour. Biological mechanisms leading to development of NHL are not clearly understood. Transforming growth factor-beta1 (TGF-beta1) influences B cell growth and developmen...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2005.12.010

    authors： Mazur G,Bogunia-Kubik K,Wrobel T,Kuliczkowski K,Lange A

    更新日期：2006-02-07 00:00:00

  • Retinal pigment epithelium-secretome: A diabetic retinopathy perspective.

    abstract：:Diabetic retinopathy is a major complication of diabetes mellitus that can lead to retinal vascular abnormalities and visual impairment. While retinal endothelial pathology is well studied, retinal pigment epithelium (RPE) layer modifications and the patho-physiological regulations are not widely understood. The RPE i...

    journal_title：Cytokine

    pub_type： 杂志文章,评审

    doi：10.1016/j.cyto.2017.02.013

    authors： Ponnalagu M,Subramani M,Jayadev C,Shetty R,Das D

    更新日期：2017-07-01 00:00:00

  • The conundrum of interferon-β non-responsiveness in relapsing-remitting multiple sclerosis.

    abstract：:A series of controlled clinical trials have shown that exogenous interferon-beta (IFN-β) benefits patients with relapsing-remitting multiple sclerosis (RRMS) by reducing relapse rate, disability progression, and the formation of new brain and spinal cord lesions on magnetic resonance imaging (MRI) scans. Unfortunately...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2015.01.004

    authors： Huber AK,Duncker PC,Irani DN

    更新日期：2015-08-01 00:00:00

  • History of interleukin-4.

    abstract：:The history of the discovery and the development of our knowledge of IL-4 exemplifies the path of progress in biomedical science. There are unanticipated twists and turns although progress is made, sometimes quickly, other times far too slowly. Illustrative is the extended time from the first report of IL-4 in 1982 to...

    journal_title：Cytokine

    pub_type： 杂志文章,评审

    doi：10.1016/j.cyto.2015.01.038

    authors： Paul WE

    更新日期：2015-09-01 00:00:00

  • IL-4, but not IL-13, modulates TARC (thymus and activation-regulated chemokine)/CCL17 and IP-10 (interferon-induced protein of 10kDA)/CXCL10 release by TNF-alpha and IFN-gamma in HaCaT cell line.

    abstract：:It is known that both interleukin-4 (IL-4) and IL-13 are produced by Th2-type cells and share similar biological functions with each other. However, recently accumulated evidences have revealed that IL-4 may be involved in the Th1-type response. Both thymus and activation-regulated chemokine (TARC/CCL17), a ligand for...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1006/cyto.2002.1965

    authors： Kakinuma T,Nakamura K,Wakugawa M,Yano S,Saeki H,Torii H,Komine M,Asahina A,Tamaki K

    更新日期：2002-10-07 00:00:00

  • Modulation by colony stimulating factors of human epithelial colon cancer cell apoptosis.

    abstract：:Colony stimulating factors (CSF) promote leukocyte survival by reducing apoptotic cell death. However, their effects on non-leukocyte cell types are unclear. Reduced apoptosis in colon epithelial cells is thought to contribute to the initiation of cancer. Here, we report diminished spontaneous apoptosis of human colon...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1006/cyto.2002.1999

    authors： Calatayud S,Warner TD,Breese EJ,Mitchell JA

    更新日期：2002-11-24 00:00:00

  • Comparative moleculo-immunological analysis of swamp- and riverine-type water buffaloes responses.

    abstract：:This moleculo-epidemiological and immunological study through cytokine response assessment was done to know the dynamics of cytokines in the initiation, persistence and association to physiological changes of a particular pathogen in water buffaloes. This is important to understand the magnitude and behavior of diseas...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2009.02.006

    authors： Mingala CN,Konnai S,Cruz LC,Onuma M,Ohashi K

    更新日期：2009-05-01 00:00:00

  • Biological activity of ovine IL-23 expressed using a foot-and-mouth disease virus 2A self-cleaving peptide.

    abstract：:Hetero-dimeric cytokines often require equi-molar expression of both subunits to achieve biological activity. Previously, we expressed ovine IL-12 p40 and p35 linked using a self-cleaving 2A peptide from foot-and-mouth disease virus (FMDV). We now generated a new improved vector for the expression of hetero-dimeric cy...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2013.01.014

    authors： Yen HH,Scheerlinck JP

    更新日期：2013-03-01 00:00:00

  • Reciprocal regulation of 17beta-estradiol, interleukin-6 and interleukin-8 during growth and progression of epithelial ovarian cancer.

    abstract：:Estrogens have been associated with risk for epithelial ovarian cancer (OVCA). Both IL-6 and IL-8 are also likely involved in the progression of OVCA. In order to discover the underline molecular mechanism, we investigated the modulation of estrogen and two cytokines in the growth and progression of epithelial OVCA. I...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2009.03.013

    authors： Yang J,Wang Y,Gao Y,Shao J,Zhang XJ,Yao Z

    更新日期：2009-06-01 00:00:00

  • CCAAT/enhancer binding protein is involved in the expression of the tumour necrosis factor gene in human monocytes.

    abstract：:Within the human TNF promoter we have identified two sites at positions -189 and -101 that show C/EBP specific binding of nuclear proteins from cells of the human monocytic line Mono Mac 6. Supershift analysis with anti C/EBP antibodies revealed that the complexes formed consist of both C/EBP alpha and C/EBP beta. Whe...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1006/cyto.1996.0046

    authors： Wedel A,Sulski G,Ziegler-Heitbrock HW

    更新日期：1996-05-01 00:00:00

  • IL-17/Th17 targeting: on the road to prevent chronic destructive arthritis?

    abstract：:Interleukin-17A (IL-17A) contributes to the pathogenesis of arthritis. Data from experimental arthritis indicate IL-17 receptor signaling as a critical pathway in turning an acute synovitis into a chronic destructive arthritis. The identification of six IL-17 family members (IL-17A-F) may extend the role of this novel...

    journal_title：Cytokine

    pub_type： 杂志文章,评审

    doi：10.1016/j.cyto.2007.09.014

    authors： Lubberts E

    更新日期：2008-02-01 00:00:00

  • MERS-CoV infection is associated with downregulation of genes encoding Th1 and Th2 cytokines/chemokines and elevated inflammatory innate immune response in the lower respiratory tract.

    abstract：:MERS-CoV, a highly pathogenic virus in humans, is associated with high morbidity and case fatality. Inflammatory responses have a significant impact on MERS-CoV pathogenesis and disease outcome. However, CD4+ T-cell induced immune responses during acute MERS-CoV infection are barely detectable, with potent inhibition ...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2019.154895

    authors： Alosaimi B,Hamed ME,Naeem A,Alsharef AA,AlQahtani SY,AlDosari KM,Alamri AA,Al-Eisa K,Khojah T,Assiri AM,Enani MA

    更新日期：2020-02-01 00:00:00

  • IL17 and IFNgamma production by peripheral blood mononuclear cells from clinically isolated syndrome to secondary progressive multiple sclerosis.

    abstract：:We evaluated the spontaneous IL17, IFNgamma and IL10 production by peripheral blood mononuclear cells from patients affected by clinically isolated syndromes (CIS) suggestive of multiple sclerosis (MS) both in acute phase and in remission, relapsing remitting MS (RRMS) both in relapse and in remission, not-relapsing s...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2008.08.007

    authors： Frisullo G,Nociti V,Iorio R,Patanella AK,Marti A,Caggiula M,Mirabella M,Tonali PA,Batocchi AP

    更新日期：2008-10-01 00:00:00

  • Human cancer cells have specifically lost the ability to induce the synergistic state caused by tumor necrosis factor plus interferon-beta.

    abstract：:Tumor necrosis factor (TNF) and the members of the interferon (IFN) family are major inducible cytokines that function to counteract viral infections or cellular transformation. Recently, our lab has characterized a novel antiviral state which is induced in primary human fibroblasts by co-treatment with TNF plus IFNbe...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2009.06.006

    authors： Bartee E,McFadden G

    更新日期：2009-09-01 00:00:00

  • Preferential production of IL-12 by peritoneal macrophages activated by liposomes prepared from neoglycolipids containing oligomannose residues.

    abstract：:The present study demonstrates that liposomes coated with synthesized neoglycolipids constructed from mannotriose and dipalmitoylphosphatidylethanolamine (Man3-DPPE) activate peritoneal macrophages (PEMs) to up-regulate expression of co-stimulatory molecules and preferentially secrete IL-12. Injection of Man3-DPPE-coa...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2007.10.005

    authors： Takagi H,Furuya N,Kojima N

    更新日期：2007-12-01 00:00:00

  • Human CC chemokine CCL23, a ligand for CCR1, induces endothelial cell migration and promotes angiogenesis.

    abstract：:A number of chemokines induce angiogenesis and endothelial cells express several chemokine receptors. To date, only a limited number of CC chemokines for CCR1 have been reported to induce angiogenic responses. We investigated the ability of CCL23 (also known as MPIF-1, MIP-3, or CKbeta8) to promote angiogenesis, which...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2005.01.018

    authors： Hwang J,Son KN,Kim CW,Ko J,Na DS,Kwon BS,Gho YS,Kim J

    更新日期：2005-06-07 00:00:00

  • Cross reactivity of S. aureus to murine cytokine assays: A source of discrepancy.

    abstract：:Staphylococcus aureus is one of the versatile Gram positive bacteria causing a range of diseases. Upon challenge, host immune cells recognize S. aureus and mount diverse immune responses including production of pro-inflammatory cytokines such as IL-1β and TNF-α. These cytokines are important mediators of inflammation ...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2016.03.007

    authors： Javed N,Xue G,Lu A,Xing Y,Iwakura Y,Xiao H,Lecoeur H,Späth GF,Meng G

    更新日期：2016-05-01 00:00:00

  • Decreased C-reactive protein-induced resistin production in human monocytes by simvastatin.

    abstract：BACKGROUND:Resistin is a novel cysteine-rich protein that plays a role in the development of insulin resistance and atherosclerosis in rodents, while its role in humans is unclear. C-reactive protein (CRP) is an important risk predictor for coronary heart disease, and it can also modify the expression of genes involved...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2007.09.011

    authors： Hu WL,Qiao SB,Li JJ

    更新日期：2007-12-01 00:00:00

  • Quantification and in situ localization of MCP-1 mRNA and its relation to the immune response of renal cell carcinoma.

    abstract：:Malignant tumours are usually accompanied by an immune response. Chemokines such as MCP-1 have been claimed to be potent inducers of such tumour-associated reactions. In the present study MCP-1 mRNA was quantified by competitive reverse transcription polymerase reaction and localised by in situ hybridisation in renal ...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1006/cyto.2000.0823

    authors： Hemmerlein B,Johanns U,Kugler A,Reffelmann M,Radzun HJ

    更新日期：2001-02-21 00:00:00

  • IL-10 promotes resistance to apoptosis and metastatic potential in lung tumor cell lines.

    abstract：:Treatment of non-small cell lung carcinoma (NSCLC) remains at a disappointingly low success rate. Not only is metastatic spread common in NSCLC, but therapeutic success decreases dramatically once metastases are present. Understanding factors which contribute to poor prognosis in NSCLC is critical for development of m...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1016/j.cyto.2009.11.015

    authors： Zeng L,O'Connor C,Zhang J,Kaplan AM,Cohen DA

    更新日期：2010-03-01 00:00:00

  • Constitutive expression of IL-6-LIKE cytokines in normal bone marrow: implications for pathophysiology of myeloma.

    abstract：:Myeloma is a neoplasm thought to "home" to bone marrow. However, evidence for bone-marrow-specific receptors or adhesion molecules expressed on myeloma cells is scanty. Initial myeloma expansion is thought to be due to IL-6 and/or related cytokines. Previous determinations of cytokine expression in bone marrow were pe...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1006/cyto.2000.0748

    authors： Barton BE,Murphy TF

    更新日期：2000-10-01 00:00:00

  • Production of tumour necrosis factor alpha by primary cultured rat alveolar epithelial cells.

    abstract：:Tumour necrosis factor alpha(TNF-alpha) is one of the most important pro-inflammatory cytokines, which plays an important role in host defense and acute inflammation related to tissue injury. The major source of TNF-alpha has been shown to be immune cells such as macrophages and neutrophils. In the present study, we d...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1006/cyto.1999.0656

    authors： McRitchie DI,Isowa N,Edelson JD,Xavier AM,Cai L,Man HY,Wang YT,Keshavjee SH,Slutsky AS,Liu M

    更新日期：2000-06-01 00:00:00

  • LERK-7: a ligand of the Eph-related kinases is developmentally regulated in the brain.

    abstract：:The eph family is the largest subfamily of receptor tyrosine kinases (RTKs). Members of this subfamily display specific expression in the developing and adult brain. Recently, cDNAs encoding membrane bound ligands for these receptors have been identified which we have termed LERKs (ligand for eph-related kinases). We ...

    journal_title：Cytokine

    pub_type： 杂志文章

    doi：10.1006/cyto.1997.0199

    authors： Kozlosky CJ,VandenBos T,Park L,Cerretti DP,Carpenter MK

    更新日期：1997-08-01 00:00:00