Abstract:
:It is known that both interleukin-4 (IL-4) and IL-13 are produced by Th2-type cells and share similar biological functions with each other. However, recently accumulated evidences have revealed that IL-4 may be involved in the Th1-type response. Both thymus and activation-regulated chemokine (TARC/CCL17), a ligand for CC chemokine receptor 4 that is mainly expressed on Th2-type cells, and interferon-induced protein of 10kDa (IP-10/CXCL10), a ligand for CXC chemokine receptor 3 that is mainly expressed on Th1-type cells, are produced by keratinocytes after the stimulation with the primary cytokines such as tumor necrotic factor-alpha (TNF-alpha) and/or interferon-gamma (IFN-gamma). In this study, we investigated the regulation of TARC or IP-10 production from HaCaT cells, an immortalized human keratinocyte cell line, after stimulation with TNF-alpha, IFN-gamma, IL-4 and/or IL-13. Without stimulation, HaCaT cells did not produce TARC. When both TNF-alpha and IFN-gamma were added, they increased synergistically (P<0.003). In addition, when HaCaT cells were stimulated with IL-4, but not IL-13, in combination with TNF-alpha and IFN-gamma, the supernatant TARC levels significantly decreased compared to those with both TNF-alpha and IFN-gamma (P<0.009). This inhibition was completely abolished with the addition of neutralizing anti-IL-4 antibody. The supernatant IP-10 levels also increased synergistically by stimulation with TNF-alpha and IFN-gamma for 24h (P<0.001). When IL-4, but not IL-13, was added to the medium and the cells were co-cultured with these cytokines, the IP-10 levels significantly increased compared to those with both TNF-alpha and IFN-gamma (P<0.04). Furthermore, the effects of IL-4 on TARC and IP-10 production in these cells were detected in a dose-dependent manner. These data strongly suggest that IL-4 may act not only as a mediator of Th1-type response but also as a down-regulator of Th2-type response in terms of the regulation of chemokine production by HaCaT cells.
journal_name
Cytokinejournal_title
Cytokineauthors
Kakinuma T,Nakamura K,Wakugawa M,Yano S,Saeki H,Torii H,Komine M,Asahina A,Tamaki Kdoi
10.1006/cyto.2002.1965keywords:
subject
Has Abstractpub_date
2002-10-07 00:00:00pages
1-6issue
1eissn
1043-4666issn
1096-0023pii
S1043466602919654journal_volume
20pub_type
杂志文章相关文献
CYTOKINE文献大全abstract::Asthma and type I diabetes are major causes of chronic illness in childhood which, according to the current paradigm, have mutually antagonistic immunopathologies. Nonetheless, the disorders appear to preferably coexist both on population and individual levels. To assess whether children with asthma and type I diabete...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2006.04.015
更新日期:2006-05-01 00:00:00
abstract::Tumor necrosis factor (TNF) and the members of the interferon (IFN) family are major inducible cytokines that function to counteract viral infections or cellular transformation. Recently, our lab has characterized a novel antiviral state which is induced in primary human fibroblasts by co-treatment with TNF plus IFNbe...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2009.06.006
更新日期:2009-09-01 00:00:00
abstract::Interleukin-21 (IL-21) is an important immune cytokine that was well characterized in human and mammals, but little is known in fish. In present study, an IL-21 homologue was cloned and well characterized from Tetraodon nigroviridis. The full-length Tetraodon IL-21 cDNA was 849bp in size, containing an open reading fr...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2006.07.010
更新日期:2006-08-01 00:00:00
abstract:INTRODUCTION:Adipocytokines represent a molecular link between metabolic factors and vascular function. The purpose of our study was to investigate the relationship between adiponectin, leptin and renal function parameters in patients with glomerular filtration rate (GFR) above 90 ml/min/m(2) and type 2 diabetes. MATE...
journal_title:Cytokine
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1016/j.cyto.2009.01.001
更新日期:2009-04-01 00:00:00
abstract::We have studied the effects of rhTGF-beta2, dexamethanosome (dex) and 1,25(OH)2D3 on human bone marrow stromal (HBMS) cells in long-term culture. A fraction on HBMS expressed type I collagen (Col I) and osteopontin, and transient treatment (48 h) with dex increased the number of alkaline phosphatase (ALP) positive cel...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.1997.0209
更新日期:1997-08-01 00:00:00
abstract::We have previously cloned the mouse platelet basic protein (mPBP), a homologue of human PBP, from mouse thymic stromal cells. Using EST alignment and RT-PCR, the rat homologue of human and mouse PBP was cloned from lung and named as rPBP. The complete open reading frame and part of the 3'- and 5'-non-coding regions we...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2003.12.007
更新日期:2004-04-07 00:00:00
abstract::Interleukin-1beta (IL-1beta) is a pleiotropic cytokine that can induce several cellular signal transduction pathways. Here, we show that IL-1beta can induce cell cycle arrest and differentiation in the human medullary thyroid carcinoma (MTC) cell line, TT. IL-1beta induces cell cycle arrest accompanied by morphologica...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2004.10.005
更新日期:2005-02-07 00:00:00
abstract::In hypertension studies, anti-inflammatory cytokine interleukin-10 (IL-10) has been shown to prevent angiotensin II (Ang II)-induced vasoconstriction and regulate vascular function by down-regulating pro-inflammatory cytokine and superoxide production in vascular cells. However, little is known about the mechanism beh...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2015.09.002
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:Recently, attenuation of anti-inflammatory and increase of pro-inflammatory mediators was demonstrated in individuals with Down syndrome (DS) in comparison with euploid patients during periodontal disease (PD), suggesting a shift to a more aggressive inflammation in DS. AIM:To determine the influence of DS ...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2012.08.020
更新日期:2012-12-01 00:00:00
abstract::Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract infection and hospitalization in infants. In spite of the enormous clinical burden caused by RSV infections, there remains no efficacious RSV vaccine. CD8 T cells mediate viral clearance as well as provide protection against a secondary ...
journal_title:Cytokine
pub_type: 杂志文章,评审
doi:10.1016/j.cyto.2018.07.012
更新日期:2020-09-01 00:00:00
abstract::Cytokine balance alterations are responsible for some of the systemic and hepatic manifestations of alcoholism. The present study was aimed to evaluate the influence of both acute alcohol abstinence (in alcoholics) and acute alcohol intake (in healthy subjects) on serum IL-6, IL-8, IL-10, and IL-12 levels. Serum cytok...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.2000.0715
更新日期:2000-09-01 00:00:00
abstract::The in vivo influence of recombinant human erythropoietin (rhEpo) and iron on human neutrophil (PMN) antimicrobial function was assessed. A total of 21 preterm infants were randomized to receive either 200 U/kg/other day of rHuEPO+12 mg/kg/day of iron (EPO+high Fe, seven infants) or 200 U/kg/other day of rhEPO+4 mg/kg...
journal_title:Cytokine
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1006/cyto.1998.0393
更新日期:1999-01-01 00:00:00
abstract::Interleukin(IL)-2 and IL-7 are cytokines with important functions related to CD4(+) lymphocyte proliferation, differentiation and survival. Depending on doses, they theoretically activate regulatory (Treg) and/or effector T cells (Teff) and thus may be indicated with different therapeutic objectives. In this study we ...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2014.05.021
更新日期:2014-09-01 00:00:00
abstract::Parasites of the genus Leishmania cause the disease leishmaniasis. As the sandfly vector transfers the promastigotes into the skin of the human host, the infection is either cured or exacerbated. In the process, there emerge several unsolved paradoxes of leishmaniasis. Chronologically, as the infections starts in skin...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2020.155304
更新日期:2020-09-29 00:00:00
abstract::Interleukin (IL)-6, a multifunctional cytokine, is widely used as an index for illnesses such as inflammatory and autoimmune disorders, coronary artery disease, neurological disease, and gestational problems. It is thus very important to be able to precisely quantify the level of IL-6 for disease diagnosis and any sub...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2010.04.004
更新日期:2010-07-01 00:00:00
abstract::Fifty-five adult patients with primary bone tumour, 27 benign and 28 malignant tumours, were assayed for leukaemia inhibitory factor (LIF) in urine and serum samples. Supernatant was obtained from primary tumour tissue cultures in 24 cases (14 benign, 10 malignant tumours). LIF was found in 11 urine samples (16.7%, 1 ...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.1997.0264
更新日期:1998-02-01 00:00:00
abstract::Interleukin (IL)-33 (or IL-1F11) was recently identified as a ligand for the orphan IL-1 receptor family member T1/ST2 (ST2). IL-33 belongs to the IL-1 cytokine family and, upon binding to ST2, induces intracellular signals similar to those utilized by IL-1. The effects of other IL-1 family cytokines are mediated by t...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2008.03.008
更新日期:2008-06-01 00:00:00
abstract::This study was aimed to evaluate the effects on some inflammatory markers of two dialytic treatments [bicarbonate dialysis (BHD) and hemodiafiltration (HDF)] in patients with severe chronic kidney disease. We evaluated: blood glucose, homeostasis model assessment insulin resistance index, homocistein (Hcs), high sensi...
journal_title:Cytokine
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1016/j.cyto.2016.12.026
更新日期:2017-04-01 00:00:00
abstract::Interferon-gamma (IFN-gamma) is a potent immunomodulatory cytokine whose physiological roles are modulated by the extracellular matrix. Here, circular dichroism and fluorescence spectroscopic techniques are used to demonstrate that low molecular weight heparin and chondroitin sulfate cause significant changes in secon...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1006/cyto.1999.0592
更新日期:2000-05-01 00:00:00
abstract::Human co-infection by helminth species is frequent, but their consequences are mostly unknown. Here, we investigate the impact of Strongyloides venezuelensis co-infection on the immune response, schistosome burden, and the associated pathology of schistosomiasis in mice. Co-infection did not alter the schistosome para...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2019.154931
更新日期:2020-03-01 00:00:00
abstract::Leptin-deficient ob/ob mice exhibit several metabolic and immune abnormalities, including thymus atrophy and markedly reduced inflammatory responses. We evaluated whether transplantation of wild-type (WT) white adipose tissue (WAT) into ob/ob mice could mimic the effect of recombinant leptin administration in normaliz...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2007.02.001
更新日期:2006-12-01 00:00:00
abstract::A well-known association between vitamin D(3) and infection with Mycobacterium tuberculosis has previously been reported, but little is known regarding the underlying mechanisms. We have investigated how 1,25-dihydroxyvitamin D(3) [1,25(OH)(2)D(3)] affects the proinflammatory cytokine production induced by M. tubercul...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2011.04.016
更新日期:2011-08-01 00:00:00
abstract:AIMS:There is interest in using blood C-reactive protein (CRP) to predict adverse prognosis outcomes patients with type 2 diabetes. This meta-analysis aimed to investigate the association between elevated baseline CRP level and unfavorable outcomes in type 2 diabetes patients. MATERIALS/METHODS:PubMed and Embase datab...
journal_title:Cytokine
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.cyto.2019.02.005
更新日期:2019-05-01 00:00:00
abstract::TNF-related apoptosis inducing ligand (TRAIL) plasma levels was measured in plasma samples obtained 1h (time 1) and 2-3 days (time 2) after delivery in a group of healthy women (n=17) who underwent cesarean delivery, and showed a significantly increase from time 1 (39.3 pg/ml median; 41.2 ± 15.9 mean ± SD) to time 2 (...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2013.05.005
更新日期:2013-10-01 00:00:00
abstract::The present study aimed to assess the kinetics of cytokine release and compare the accuracy of serum biomarkers for the diagnosis of macrophage activation syndrome (MAS) associated with Kawasaki disease (KD). Serum neopterin, interleukin (IL)-18, IL-6 and soluble tumour necrosis factor receptor type I (sTNFR-I) and sT...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2019.03.001
更新日期:2019-07-01 00:00:00
abstract::In order to understand the cellular disease mechanisms of osteoarthritic cartilage degeneration it is of primary importance to understand both the anabolic and the catabolic processes going on in parallel in the diseased tissue. In this study, we have applied cDNA-array technology (Clontech) to study gene expression p...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2005.04.009
更新日期:2005-08-07 00:00:00
abstract::Among New Mexican Hispanic women, breast cancer is detected at a more advanced stage than compared to Non-Hispanic White women. One central factor that has been little studied is the role of critical cytokines. We genotyped incident breast cancer cases and their age-, gender- and smoking-matched controls (N=40 matched...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2010.03.014
更新日期:2010-07-01 00:00:00
abstract:OBJECTIVE:Epicardial adipose tissue (EAT) produces a wide range of adipokines and has recently been linked to the physiopathology of cardiovascular (CV) and metabolic diseases. We aimed to study whether EAT and subcutaneous (SAT) adiponectin and leptin expression levels are associated with CV complications during long-...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2012.08.012
更新日期:2012-12-01 00:00:00
abstract::The aim of this study was to characterize the association between adipocyte enlargement and circulating levels of serum amyloid A (SAA). Furthermore, we wanted to search for possible associations with measures of glycemic control and levels of circulating adipokines and/or inflammatory markers in men and women with a ...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2009.08.005
更新日期:2009-12-01 00:00:00
abstract::LIGHT (lymphotoxin-like inducible protein that competes with glycoprotein D for binding herpesvirus entry mediator on T cells) is a recently identified of the tumor necrosis factor alpha (TNF-alpha) ligand superfamily. We wanted to establish whether the presence of chronic viral hepatitis could be implicated in enhanc...
journal_title:Cytokine
pub_type: 杂志文章
doi:10.1016/j.cyto.2007.07.189
更新日期:2007-09-01 00:00:00