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Changes in rat melanin-concentrating hormone and dynorphin messenger ribonucleic acids induced by food deprivation.

Abstract：

:Melanin-concentrating hormone (MCH) and dynorphin genes are expressed in two discrete neuron populations of the rat lateral hypothalamus. Their roles remain hypothetical in mammals. In order to analyse changes in MCH and dynorphin gene expression, a multiplex competitive semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) was developed to assay their mRNAs. This technique was used to examine MCH and dynorphin mRNA content in 24-h and 48-h food-deprived rats compared to controls. A two-fold induction of dynorphin mRNA by 24 h, followed by a sharp decrease at 48 h were observed. A moderate increase in MCH mRNA content was noticed by 24 h; 48 h of fasting restored the control levels.

journal_name

Neuropeptides

journal_title

Neuropeptides

authors

Hervé C,Fellmann D

doi

10.1016/s0143-4179(97)90054-8

subject

Has Abstract

pub_date

1997-06-01 00:00:00

pages

237-42

issue

3

eissn

0143-4179

issn

1532-2785

pii

S0143-4179(97)90054-8

journal_volume

31

pub_type

杂志文章

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