Soluble sperm extract triggers inositol 1,4,5-trisphosphate-induced Ca2+ release in the oocytes of the sea urchin, Anthocidaris crassispina.

abstract：:Epithelial tubules, consisting of the epithelial cell sheet with a central lumen, are the basic structure of many organs. Mechanical forces play an important role in epithelial tubulogenesis; however, little is known about the mechanisms controlling the mechanical forces during epithelial tubule morphogenesis. Solo (a...

journal_title：Cell structure and function

authors： Nishimura R,Kato K,Fujiwara S,Ohashi K,Mizuno K

更新日期：2018-06-02 00:00:00

abstract：:The effects of heavy ion microbeams on muscle fibers isolated from mouse skeletal muscles were examined by electron microscopy. The plasma membranes of heavy ion beam-irradiated areas of muscle fibers showed irregular protrusions and invaginations. In the cytoplasm, an irregular distribution of microfilaments was foun...

journal_title：Cell structure and function

authors： Hino M,Wada S,Tajika Y,Morimura Y,Hamada N,Funayama T,Sakashita T,Kakizaki T,Kobayashi Y,Yorifuji H

更新日期：2007-01-01 00:00:00

abstract：:Here, we would like to point out important milestones in the study of nuclear radial positioning and gene expression during differentiation processes. In addition, changes in the histone signature that significantly precede various differentiation pathways are reviewed. We address the regulatory functions of chromatin...

journal_title：Cell structure and function

authors： Harnicarová Horáková A,Bártová E,Kozubek S

更新日期：2010-01-01 00:00:00

abstract：:Hepatic stellate cells (HSCs), a mesenchymal cell type in hepatic parenchyma, have unique features with respect to their cellular origin, morphology, and function. Normal, quiescent HSCs function as major vitamin A-storing cells containing over 80% of total vitamin A in the body to maintain vitamin A homeostasis. HSCs...

journal_title：Cell structure and function

authors： Sato M,Suzuki S,Senoo H

更新日期：2003-04-01 00:00:00

abstract：:Mouse embryonic stem (mES) cells can be maintained in undifferentiated state in the presence of a cytokine, leukemia inhibitory factor (LIF). Many investigators found that STAT3 activation is important for the maintenance of pluripotency by LIF. However, the downstream pathways of STAT3 activation are still unknown. T...

journal_title：Cell structure and function

authors： Ko SY,Kang HY,Lee HS,Han SY,Hong SH

更新日期：2006-01-01 00:00:00

abstract：:All trans retinoic acid (ATRA), a differentiation inducer for human myeloid NB4 cells, induced accumulation of lipid droplet as determined by positivity of Nile Red and Oil Red O in this cell line. Granulocyte colony-stimulating factor (G-CSF), although not having detectable effect by itself, exerted the additive effe...

journal_title：Cell structure and function

authors： Inazawa Y,Nakatsu M,Yasugi E,Saeki K,Yuo A

更新日期：2003-10-01 00:00:00

abstract：:Histone chaperones are a group of histone-binding proteins that facilitate the assembly of nucleosomes, the fundamental structural units of chromatin in eukaryotes. In nucleosome assembly, deposition of a histone H3-H4 tetramer onto DNA is the first and critical step, which is mediated by the histone chaperones HIRA a...

journal_title：Cell structure and function

authors： Zhu R,Iwabuchi M,Ohsumi K

更新日期：2017-01-01 00:00:00

abstract：:Addition of hemin to the nuclear reconstitution system of Xenopus interphase egg extract using sperm head chromatin resulted in abnormal pseudonuclei exhibiting flattened membrane patches randomly distributed both on the surface and inside the nuclei. The structures that resembled nuclear pores were observed on these ...

journal_title：Cell structure and function

authors： Nishiyama R,Ikami M,Yamao F,Tsuchida S,Yagura T

更新日期：1998-10-01 00:00:00

abstract：:Newly prepared phosphatidylethanolamine (PE) conjugates of glycosaminoglycans (GAGs) enabled us to immobilize GAGs to solid phase through hydrophobic interaction. Using these compounds, called GAG-PEs, we studied the effects of GAGs immobilized to culture plates coated with various extracellular matrix (ECM) proteins ...

journal_title：Cell structure and function

authors： Kato S,Sugiura N,Kimata K,Kujiraoka T,Toyoda J,Akamatsu N

更新日期：1995-06-01 00:00:00

abstract：:Effects of kinase inhibitors on the preprophase band of microtubules in onion (Allium cepa L.) root tip cells were examined. Bundled microtubules in preprophase bands were dispersed on the cell cortex when onion seedlings were incubated with 2.5-5.0 mM 6-dimethylaminopurine. Fifteen min was enough for the bundled micr...

journal_title：Cell structure and function

authors： Nogami A,Mineyuki Y

更新日期：1999-10-01 00:00:00

abstract：:High voltage electric pulses, 1000 V/cm, were found to induce cell fusion efficiently when delivered to cells growing in a monolayer culture. The maximum yield of fused cells was about 30% of cells remaining after treatment. Colonies of interspecies hybrid cells between mouse and others also appeared with a frequency ...

journal_title：Cell structure and function

authors： Ishizaki K,Chang HR,Eguchi T,Ikenaga M

更新日期：1989-04-01 00:00:00

abstract：:A voltage-dependent and Ca2(+)-activated cation channel recently found in the vacuolar membrane of the yeast Saccharomyces cerevisiae was incorporated into planar lipid bilayers and further characterized in macroscopic and single channel levels. Single channel conductances for various cations were in the order: NH4+ g...

journal_title：Cell structure and function

authors： Sato M,Tanifuji M,Kasai M

更新日期：1989-12-01 00:00:00

abstract：:Mouse FM3A cells propagated well in serum-free medium containing 0.5% serum albumin and 1 microgram of insulin/ml. The vitamin B12 (B12) requirement of the cells depended on the population density. This requirement disappeared when a sufficiently large cell population was present. A combination of 1-100 ng of B12/ml a...

journal_title：Cell structure and function

authors： Matsuya Y,Yamane I

更新日期：1986-03-01 00:00:00

abstract：:Cofilin, a 21kDa actin-binding protein, binds to F-actin in a 1:1 molar ratio of cofilin to actin molecule (Nishida, E., S. Maekawa, and H. Sakai, Biochemistry, 23, 5307-5313, 1984) and is capable of controlling actin polymerization and depolymerization in vitro in a pH-sensitive manner (Yonezawa, N., E. Nishida, and ...

journal_title：Cell structure and function

authors： Yonezawa N,Nishida E,Koyasu S,Maekawa S,Ohta Y,Yahara I,Sakai H

更新日期：1987-10-01 00:00:00

abstract：:Nonmuscle myosin II activity is regulated by phosphorylation of the myosin II regulatory light chain (MRLC) at Ser19 or at both Thr18 and Ser19, and the phosphorylation of MRLC promotes the contractility and stability of actomyosin. To analyze the states of MRLC phosphorylation at the leading edge in the motile HeLa c...

journal_title：Cell structure and function

authors： Uchimura T,Fumoto K,Yamamoto Y,Ueda K,Hosoya H

更新日期：2002-12-01 00:00:00

abstract：:Upon tryptic digestion of synaptosomes, ATPase activities decreased in the order of Cl(-)-ATPase greater than or equal to Na+,K+-ATPase greater than anion-insensitive Mg2+-ATPase. Upon synaptosome treatment with hypotonic solution, Cl(-)-ATPase or anion-insensitive Mg2+-ATPase was slightly inactivated, while Na+,K+-AT...

journal_title：Cell structure and function

authors： Kunugi-Uehara Y,Hashimoto Y,Ogura C,Inagaki C,Nishino T

更新日期：1988-04-01 00:00:00

abstract：:Previously, we serially transplanted tumors that produced colony-stimulating factor (CSF) into nude mice, who developed marked granulocytosis along with tumor growth; their leukocyte counts reaching approximately one million per cu mm. The numbers of CFU-GM, CFU-E, CFU-Meg, CFU-S and BFU-E were increased in nude mice ...

journal_title：Cell structure and function

authors： Takeda K,Suda T,Sudo T,Miura Y

更新日期：1987-06-01 00:00:00

abstract：:To investigate the mechanism of myogenic differentiation, we are using quail myoblast cells (QM cells) transformed with a temperature-sensitive mutant of Rous sarcoma virus (ts-RSV), termed QM-RSV cells. At 35.5 degrees C, a permissive temperature for RSV, QM-RSV cells repeatedly proliferate without differentiation, b...

journal_title：Cell structure and function

authors： Hase H,Hirayama E,Kim J

更新日期：1996-02-01 00:00:00

abstract：:The proto-oncogene c-myc is a multifunctional gene that regulates cell division, cell growth, and apoptosis. Here we report a new function of c-myc: induction of autophagy. Autophagy is a bulk degradation system for intracellular proteins. Autophagy proceeds with characteristic morphologies, which begins with the form...

journal_title：Cell structure and function

authors： Tsuneoka M,Umata T,Kimura H,Koda Y,Nakajima M,Kosai K,Takahashi T,Takahashi Y,Yamamoto A

更新日期：2003-06-01 00:00:00

abstract：:In view of the importance of vitamin A in the human immune system and the central role of interleukin-2 (IL-2) in the proliferation of T-lymphocytes, we examined the effects of all-trans-retinoic acid (ATRA) on the protein and gene expression of IL-2 in the human T-cell line HUT-78 when stimulated with either 12-O-tet...

journal_title：Cell structure and function

authors： Goto S,Okada N,Kaneko A,Isemura M

更新日期：2008-01-01 00:00:00

abstract：:The epidermal growth factor (EGF)-induced endocytosis of its receptor is an obligatory pathway for the cellular regulation of the EGF-specific receptor (EGF-R). BNER4 is a mouse Balb/3T3 cell line transfected with human EGF-R complementary DNA (cDNA). B4/src-13 and B4/src-24 are BNER4 cells transfected with a viral on...

journal_title：Cell structure and function

authors： Nakayama Y,Hisano T,Okimoto T,Tanaka Y,Ishikawa T,Himeno M,Ono M,Kuwano M

更新日期：1994-12-01 00:00:00

abstract：:Embryonic stem (ES) cells are a pluripotent cell line derived from the inner cell mass of the mouse blastocyst. The mRNA levels of several growth-related genes were examined in ES cells by Northern blot analysis under several different growth conditions. In a coculture of ES cells with the mouse fibroblast cell line, ...

journal_title：Cell structure and function

authors： Katsube K,Shimizu N

更新日期：1991-10-01 00:00:00

abstract：:DNA synthesis of WF-1 fibroblasts derived from a patient with Werner's syndrome was stimulated by fetal calf serum and adult human serum but not by various mitogens including epidermal growth factor, platelet-derived growth factor (PDGF), fibroblast growth factor, insulin and 12-O-tetradecanoylphorbol-13-acetate (TPA)...

journal_title：Cell structure and function

authors： Hirai M,Amagai M,Tajima S,Nishikawa T,Shimizu N

更新日期：1988-12-01 00:00:00

abstract：:Hepatic stellate cells (HSCs) were changed in their morphology, proliferative activity, and functions by culturing on type I collagen gel, as compared to the culture on polystyrene surface. HSCs have been found to produce extracellular matrix components and matrix metalloproteinases (MMPs). In this study, we have asse...

journal_title：Cell structure and function

authors： Li YL,Sato M,Kojima N,Miura M,Senoo H

更新日期：1999-10-01 00:00:00

abstract：:Pericentric regions form epigenetically organized, silent heterochromatin structures that accumulate histone H3 lysine 9 tri-methylation (H3K9me3) and heterochromatin protein 1 (HP1), a methylated H3K9-binding protein. At pericentric regions, Suv39h is the major enzyme that generates H3K9me3. Suv39h also interacts dir...

journal_title：Cell structure and function

authors： Muramatsu D,Kimura H,Kotoshiba K,Tachibana M,Shinkai Y

更新日期：2016-12-03 00:00:00

abstract：:Two monoclonal antibodies against alpha-tubulin (YL1/2 and D2D6) were microinjected into the egg of the sand dollar Clypeaster japonicus, and their effects on cleavage of the egg were investigated. They had already been shown by immunoblotting to react specifically with egg tubulin and by immunofluorescence to stain t...

journal_title：Cell structure and function

authors： Oka MT,Arai T,Hamaguchi Y

更新日期：1990-12-01 00:00:00

abstract：:The endolymphatic sac (ES) is believed to play an important role in maintaining homeostasis in the inner ear by the absorption and endocytosis of endolymph. Megalin is a 600-kDa multiligand endocytic receptor expressed in certain types of absorptive epithelia including kidney proximal tubules. We analyzed the immunore...

journal_title：Cell structure and function

authors： Ishida T,Hatae T,Nishi N,Araki N

更新日期：2006-01-01 00:00:00

abstract：:NBT-II cells on collagen-coated substrates move rapidly and persistently, maintaining a semi-circular shape with a large lamellipodium, in a manner similar to fish keratocytes. The inhibitor of phospholipase D (PLD), n-butanol, completely blocked the migration and disturbed the characteristic localization of actin alo...

journal_title：Cell structure and function

authors： Nagasaki A,Inotsume K,Kanada M,Uyeda TQ

更新日期：2008-01-01 00:00:00

abstract：:tsJT60 is a nonlethal temperature-sensitive (ts) mutant of a Fischer rat cell line (3Y1) classified as a G0 mutant; i.e., the ts defect is not expressed within the cell growth cycle but is expressed only between the G0 and S phase. tsJT60 clones transformed with oncogenes such as adenovirus E1A, polyoma large T, polyo...

journal_title：Cell structure and function

authors： Tai YY,Ninomiya-Tsuji J,Furuoku K,Ogawa N,Ishibashi S,Shiroki K,Segawa K,Tsuchida N,Shibuya M,Ide T

更新日期：1990-12-01 00:00:00

abstract：:The putative ionophoretic action of phosphatidic acid or arachidonic acid metabolites for Ca2+ has offered an attractive explanation for stimulation-coupled mobilization of cytoplasmic Ca2+. We have examined the effects of Ca2+ ionophore and long-chain unsaturated fatty acids on the translocation of Ca2+ across the li...

journal_title：Cell structure and function

authors： Utsumi K,Nobori K,Miyahara M

更新日期：1985-06-01 00:00:00