Abstract:
:Previously, we serially transplanted tumors that produced colony-stimulating factor (CSF) into nude mice, who developed marked granulocytosis along with tumor growth; their leukocyte counts reaching approximately one million per cu mm. The numbers of CFU-GM, CFU-E, CFU-Meg, CFU-S and BFU-E were increased in nude mice bearing CSF-producing tumor. We here report that tumor-conditioned medium (TCM) derived from the CSF-producing tumors had colony-stimulating activity (CSA) and burst-promoting activity (BPA) when normal murine spleen cells as well as normal human bone marrow cells were the target cells. The activity of TCM supported multilineage colony formation in 5-fluorouracil (5-FU)-treated mouse spleen cells, in which only the primitive population of stem cells was reserved. No interleukin-3 (IL-3) activity was detected in TCM when assayed using the IL-3 dependent cell lines. We conclude that the factor in TCM acts on pluripotent stem cells and on the early progenitor stage of various cell lineages. It is distinct from IL-3.
journal_name
Cell Struct Functjournal_title
Cell structure and functionauthors
Takeda K,Suda T,Sudo T,Miura Ydoi
10.1247/csf.12.273subject
Has Abstractpub_date
1987-06-01 00:00:00pages
273-80issue
3eissn
0386-7196issn
1347-3700journal_volume
12pub_type
杂志文章abstract::The Golgi stress response is a homeostatic mechanism that controls the capacity of the Golgi apparatus in accordance with cellular demands. When the capacity of the Golgi apparatus becomes insufficient (Golgi stress), transcription levels of Golgi-related genes encoding glycosylation enzymes, a Golgi structural protei...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.16005
更新日期:2016-07-30 00:00:00
abstract::Retinoids (retinoic acid and its analogs) are widely involved in the control of cell proliferation, cell differentiation and embryonic development. A series of potent and novel synthetic retinoids named retinobenzoic acids has been developed. Retinobenzoic acids have proven to be useful tools in the investigation of t...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
doi:10.1247/csf.16.113
更新日期:1991-04-01 00:00:00
abstract::Phosphorylation of regulatory light chain (RMLC) of myosin II at Ser19/Thr18 is likely to play important roles in controlling the morphological changes seen during cell division of cultured mammalian cells. Phosphorylation of RMLC regulates the activity of myosin II, an essntial motor for cytokinesis, and phosphorylat...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
doi:10.1247/csf.26.639
更新日期:2001-12-01 00:00:00
abstract::We have demonstrated simultaneous measurement of the membrane potential and cytoplasmic free Ca2+ concentration ([Ca2+]i) by utilizing a dual-laser flow cytometer in embryonic rat hippocampal cell suspensions. Veratrine, a Na+ channel activator, induced both membrane depolarization and elevation of [Ca2+]i. These acti...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.14.669
更新日期:1989-12-01 00:00:00
abstract::Primary hepatocytes were cultured on collagen gel in serum-free, alpha-modified Eagle's minimum essential medium containing 0.1 microM insulin, 0.1 microM dexamethasone, 10 mM pyruvate and supplements such as glucagon, epinephrine or growth hormone. The activities of alkaline phosphatase, 5'-nucleotidase and gamma-glu...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.18.73
更新日期:1993-02-01 00:00:00
abstract::Tenascin-C is an oligomeric glycoprotein of the extracellular matrix that has been found to have both adhesive and anti-adhesive properties for cells. Recent elucidation of the two major TNC splice variants (320 kDa and 220 kDa) has shed light on the possibility of varying functions of the molecule based on its splici...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.26.179
更新日期:2001-06-01 00:00:00
abstract::The endolymphatic sac (ES) is believed to play an important role in maintaining homeostasis in the inner ear by the absorption and endocytosis of endolymph. Megalin is a 600-kDa multiligand endocytic receptor expressed in certain types of absorptive epithelia including kidney proximal tubules. We analyzed the immunore...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.06013
更新日期:2006-01-01 00:00:00
abstract::A strain of Saccharomyces cerevisiae that contains an integrated copy of a Xenopus cyclin A1 gene under the control of the GAL1 promoter has been constructed. On inducing expression of cyclin A1, the nuclear migration that occurs prior to division becomes aberrant. Instead of migrating to the neck between the mother c...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.22.465
更新日期:1997-08-01 00:00:00
abstract::The ETS family of transcription factors is defined by a conserved DNA-binding ETS domain that forms a winged helix-turn-helix structural motif. This family of transcription factors is involved in a diverse array of biological functions including cellular growth and differentiation, as well as organ development. Among ...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
doi:10.1247/csf.26.19
更新日期:2001-02-01 00:00:00
abstract::The amount of free calcium in the cytoplasm is important in stimulation coupled with a number of cellular functions. The putative ionophoretic action of membrane lipid metabolites on Ca2+ offers convenient explanation of the stimulation-coupled mobilization of cytoplasmic Ca2+. To analyze the ionophoretic action of th...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.10.339
更新日期:1985-12-01 00:00:00
abstract::CD9 and CD63 belong to a tetramembrane-spanning glycoprotein family called tetraspanin, and are involved in a wide variety of cellular processes, but the structure-function relationship of this family of proteins has yet to be clarified. CD9 associates with diphtheria toxin receptor (DTR), which is identical to the me...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.25.317
更新日期:2000-10-01 00:00:00
abstract::Contact reaction is the morphological expression of allogeneic recognition by hemocytes in the ascidian, Halocynthia roretzi; namely they undergo an exocytotic burst upon contact with non-self (allogeneic) hemocytes in vitro. We have found that contact reaction is accompanied by a burst of cyanide-insensitive oxygen c...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.20.81
更新日期:1995-02-01 00:00:00
abstract::Tobacco BY-2 cells were synchronized by an aphidicolin treatment, and their beta-tubulin isoforms and their mRNA were analyzed by Western, Northern and dot blottings. The relative ratio of the beta-tubulin isoforms changed with the progress of cell cycle stage. By Northern blot hybridization of poly(A)+RNAs with a clo...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.16.489
更新日期:1991-12-01 00:00:00
abstract::Histone modifications play critical roles in the epigenetic regulation of gene expression and in the maintenance of genome integrity. Acetylation and methylation of histone H3 are particularly important in gene activation and silencing. We generated and characterized a panel of mouse monoclonal antibodies that specifi...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.07035
更新日期:2008-01-01 00:00:00
abstract::The substance in sperm which induces the activation of eggs at fertilization has yet to be elucidated. Osawa et al. reported that the soluble extract of sperm (spex) causes inositol 1,4,5-trisphosphate (IP3)-induced Ca2+ release (IICR) in fertilized eggs of sea urchins when externally applied [Osawa et al., (1992). Zo...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.19.73
更新日期:1994-04-01 00:00:00
abstract::The involvement of actin filaments from the host cell on the process of invasion of trypomastigote forms of Trypanosma cruzi was analyzed in seven different cell lines. Prior incubation of all cell lines with cytochalasin D, under conditions which interfere with actin filaments, markedly inhibited parasite internaliza...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.27.91
更新日期:2002-04-01 00:00:00
abstract::Upon tryptic digestion of synaptosomes, ATPase activities decreased in the order of Cl(-)-ATPase greater than or equal to Na+,K+-ATPase greater than anion-insensitive Mg2+-ATPase. Upon synaptosome treatment with hypotonic solution, Cl(-)-ATPase or anion-insensitive Mg2+-ATPase was slightly inactivated, while Na+,K+-AT...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.13.105
更新日期:1988-04-01 00:00:00
abstract::Two types of DNA ligase, I and II, have been purified approximately 4,000-fold from mouse testes and 500-fold from nuclei of mouse spermatocytes. DNA ligase I and II consisted of single polypeptides with molecular weights of 95,000 and 65,000, respectively, according to the estimation by SDS-polyacrylamide gel electro...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.15.67
更新日期:1990-02-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.22.139
更新日期:1997-02-01 00:00:00
abstract::NBT-II cells on collagen-coated substrates move rapidly and persistently, maintaining a semi-circular shape with a large lamellipodium, in a manner similar to fish keratocytes. The inhibitor of phospholipase D (PLD), n-butanol, completely blocked the migration and disturbed the characteristic localization of actin alo...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.07042
更新日期:2008-01-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.11.9
更新日期:1986-03-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
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更新日期:1985-06-01 00:00:00
abstract::Cholera toxin binding sites in frozen sections of rat thymus were studied by indirect immunofluorescence and were found to be localized selectively in the medulla of thymic lobules. By flow cytometric analysis, two populations of thymocytes differing in size and reactivity towards cholera toxin were detected; the smal...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.12.339
更新日期:1987-08-01 00:00:00
abstract::Addition of hemin to the nuclear reconstitution system of Xenopus interphase egg extract using sperm head chromatin resulted in abnormal pseudonuclei exhibiting flattened membrane patches randomly distributed both on the surface and inside the nuclei. The structures that resembled nuclear pores were observed on these ...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.23.291
更新日期:1998-10-01 00:00:00
abstract::Synapsin I, one of the major synaptic proteins, is thought to associate with synaptic vesicles and to play a regulatory role in neurotransmitter release. In mature neurons, it is concentrated almost exclusively in presynaptic nerve endings. Here, we studied the subcellular localization of synapsin I during the develop...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.15.329
更新日期:1990-12-01 00:00:00
abstract::Cofilin, a 21kDa actin-binding protein, binds to F-actin in a 1:1 molar ratio of cofilin to actin molecule (Nishida, E., S. Maekawa, and H. Sakai, Biochemistry, 23, 5307-5313, 1984) and is capable of controlling actin polymerization and depolymerization in vitro in a pH-sensitive manner (Yonezawa, N., E. Nishida, and ...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.12.443
更新日期:1987-10-01 00:00:00
abstract::Thyroid follicles in vivo are embedded in extracellular matrix (ECM). The composing epithelial cells are in close contact with ECM at the basal side. To examine cell-to-ECM interactions, we studied adhesion, proliferation and differentiation of porcine follicle cells monolayer-cultured on type I and IV collagen, fibro...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.20.345
更新日期:1995-10-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.17.105
更新日期:1992-04-01 00:00:00
abstract::To investigate the mechanism of myogenic differentiation, we are using quail myoblast cells (QM cells) transformed with a temperature-sensitive mutant of Rous sarcoma virus (ts-RSV), termed QM-RSV cells. At 35.5 degrees C, a permissive temperature for RSV, QM-RSV cells repeatedly proliferate without differentiation, b...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.21.17
更新日期:1996-02-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.12.251
更新日期:1987-06-01 00:00:00