Abstract:
:To investigate the mechanism of myogenic differentiation, we are using quail myoblast cells (QM cells) transformed with a temperature-sensitive mutant of Rous sarcoma virus (ts-RSV), termed QM-RSV cells. At 35.5 degrees C, a permissive temperature for RSV, QM-RSV cells repeatedly proliferate without differentiation, but, at 41 degrees C, a nonpermissive temperature, myogenic differentiation proceeds. This temperature dependency of the differentiation is derived from protein kinase activity of pp60v-src, as tyrosine dephosphorylation is necessary for myogenic differentiation of QM-RSV cells. In this study, it was demonstrated that among four fusion inhibitors, aspirin, doxorubicin, HMBA and TPA, three of the inhibitors, except for TPA, inhibited myogenin gene expression. Moreover, HMBA inhibited myoblast fusion accompanying inhibition of tyrosine dephosphorylation of certain proteins, and recovered the tyrosine kinase activity of pp60v-src to a certain extent. To study the effect of HMBA on the intracellular localization of pp60v-src, detergent-soluble and detergent-resistant fractions were prepared with Triton X-100. As a result, it was shown that pp60v-src mainly exists in detergent-resistant fraction at 35.5 degrees C. While almost all of the pp60v-src at 41 degrees C exists in detergent-soluble fraction. HMBA treatment retained pp60v-src in detergent-resistant fraction even at 41 degrees C. These results suggest that HMBA inhibits myogenic differentiation of QM-RSV cells by affecting the regulation of pp60v-src.
journal_name
Cell Struct Functjournal_title
Cell structure and functionauthors
Hase H,Hirayama E,Kim Jdoi
10.1247/csf.21.17subject
Has Abstractpub_date
1996-02-01 00:00:00pages
17-25issue
1eissn
0386-7196issn
1347-3700journal_volume
21pub_type
杂志文章abstract::The role of microtubule associated proteins (MAPs) on the dynamic instability of microtubules was examined under a dark-field microscope using bovine brain tubulin purified by DEAE-Sepharose column chromatography. In the absence of MAPs, the transition from the shortening phase to the growing phase (the rescue) occurr...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.19.279
更新日期:1994-10-01 00:00:00
abstract::When human erythroleukemia cells (K562) were exposed to phorbol-12-myristate 13-acetate (PMA), phosphorylation of transferrin receptors was enhanced 5-fold with 10(-7) M PMA and 7-fold with 10(-6) M PMA, but not with 4 alpha-phorbol (5 X 10(-7) M). Stimulation took place in serine residues in the cytoplasmic domain of...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.10.95
更新日期:1985-06-01 00:00:00
abstract::A protein phosphatase inhibitor, tautomycin induces blebs on the surface of human myeloid leukemia K562 cells within 10 min. In this paper we examined the cytoskeleton of tautomycin-treated cells. In the presence of tautomycin, cells with blebs turned into segmented forms with smooth surfaces after 15 min and into smo...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.18.33
更新日期:1993-02-01 00:00:00
abstract::Murine tracheal epithelial cell lines, TM01 and TM02-3, were established from a primary culture of tracheal cells of adult transgenic mice bearing a temperature-sensitive simian virus (SV40) large T-antigen gene. Both TM01 and TM02-3 cells, which grew until confluent monolayers were formed, maintained tight contact wi...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.23.119
更新日期:1998-06-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.15.13
更新日期:1990-02-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.13.267
更新日期:1988-06-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.30.69
更新日期:2005-01-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.15.221
更新日期:1990-08-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.22.51
更新日期:1997-02-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
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更新日期:1999-10-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.19023
更新日期:2019-11-23 00:00:00
abstract::Mouse embryonic stem (mES) cells can be maintained in undifferentiated state in the presence of a cytokine, leukemia inhibitory factor (LIF). Many investigators found that STAT3 activation is important for the maintenance of pluripotency by LIF. However, the downstream pathways of STAT3 activation are still unknown. T...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.31.53
更新日期:2006-01-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.19.189
更新日期:1994-08-01 00:00:00
abstract::The proto-oncogene c-myc is a multifunctional gene that regulates cell division, cell growth, and apoptosis. Here we report a new function of c-myc: induction of autophagy. Autophagy is a bulk degradation system for intracellular proteins. Autophagy proceeds with characteristic morphologies, which begins with the form...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.28.195
更新日期:2003-06-01 00:00:00
abstract::Cholera toxin binding sites in frozen sections of rat thymus were studied by indirect immunofluorescence and were found to be localized selectively in the medulla of thymic lobules. By flow cytometric analysis, two populations of thymocytes differing in size and reactivity towards cholera toxin were detected; the smal...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.12.339
更新日期:1987-08-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.06025
更新日期:2006-01-01 00:00:00
abstract::Here we report a new method of isolating epidermal desmosomes from Xenopus laevis, and a major constituent of desmosomes designated as Xenopus desmogleins (XDsg). Isolation of desmosomes from Xenopus laevis epidermis was carried out by a two step-incubation with different concentrations of NP-40. After discontinuous s...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.29.17
更新日期:2004-02-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.16.323
更新日期:1991-08-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.17.301
更新日期:1992-10-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.07035
更新日期:2008-01-01 00:00:00
abstract::In adult rat cerebellum, HPC-1/syntaxin 1 is detected at high density on the plasma membrane of the non-synaptic region of parallel fibers in addition to the synaptic terminal membranes and the synaptic vesicles (Koh, S., Yamamoto, A., Inoue, A., Inoue, Y., Akagawa, Y., Kawamura, Y., Kawamoto, Y., and Tashiro, Y. (199...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.21.525
更新日期:1996-12-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.20.81
更新日期:1995-02-01 00:00:00
abstract::To study the effects of surface materials of cells on the behavior of other neighboring cells in a crowded culture, confluent sheets of rat 3Y1 fibroblasts were fixed and then 3Y1 cells were seeded on to them. Among confluent sheets unfixed, fixed with formalin and fixed with ethanol and an empty plastic dish surface,...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.15.257
更新日期:1990-10-01 00:00:00
abstract::The discovery of fluorescent proteins (FPs) has revolutionized cell biology. The fusion of targeting sequences to FPs enables the investigation of cellular organelles and their dynamics; however, occasionally, such fluorescent fusion proteins (FFPs) exhibit behavior different from that of the native proteins. Here, we...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.19028
更新日期:2019-12-26 00:00:00
abstract::DNA synthesis of WF-1 fibroblasts derived from a patient with Werner's syndrome was stimulated by fetal calf serum and adult human serum but not by various mitogens including epidermal growth factor, platelet-derived growth factor (PDGF), fibroblast growth factor, insulin and 12-O-tetradecanoylphorbol-13-acetate (TPA)...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.13.471
更新日期:1988-12-01 00:00:00
abstract::Upon tryptic digestion of synaptosomes, ATPase activities decreased in the order of Cl(-)-ATPase greater than or equal to Na+,K+-ATPase greater than anion-insensitive Mg2+-ATPase. Upon synaptosome treatment with hypotonic solution, Cl(-)-ATPase or anion-insensitive Mg2+-ATPase was slightly inactivated, while Na+,K+-AT...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.13.105
更新日期:1988-04-01 00:00:00
abstract::Vascular endothelial cells possess antithrombotic properties, which are determined by the balance between plasminogen activators (PAs) and PA inhibitors (PAls). A cell line, TKM-33, has been established and cloned from human umbilical vein endothelial cells, was previously reported to produce a large amount of urokina...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.24.71
更新日期:1999-04-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.17016
更新日期:2017-01-01 00:00:00
abstract::The relative fibrin-binding, fibrinolytic and fibrinogenolytic properties of single-chain pro-urokinase, an inactive proenzyme form of human urokinase purified from cultured human kidney cells, and urokinase were compared. The affinity of single-chain pro-urokinase for fibrin was much higher than that of urokinase. In...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.10.151
更新日期:1985-06-01 00:00:00
abstract::Tritrichomonas foetus strongly agglutinates human erythrocytes, thus suggesting the occurrence of an adhesin associated with its surface. Adherence was observed immediately after mixing of the parasites with erythrocytes and the intensity increased for up to 30 min. Scanning electron microscopy examination of T. foetu...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.21.245
更新日期:1996-08-01 00:00:00