Abstract:
:Two types of DNA ligase, I and II, have been purified approximately 4,000-fold from mouse testes and 500-fold from nuclei of mouse spermatocytes. DNA ligase I and II consisted of single polypeptides with molecular weights of 95,000 and 65,000, respectively, according to the estimation by SDS-polyacrylamide gel electrophoresis and the AMP-binding assay. Ligase activities were higher in premeiotic spermatogonia and spermatocytes than those in liver and bone marrow cells. Moreover, DNA ligase II showed rapid increase during meiotic prophase and a decrease in round spermatids. Since this behavior of DNA ligase II is consistent with that of m-rec and DNA polymerase beta, both of which have been shown to be involved in DNA recombination in meiotic cells, DNA ligase II might be an enzyme which works at the final step of meiotic recombination reaction.
journal_name
Cell Struct Functjournal_title
Cell structure and functionauthors
Higashitani A,Tabata S,Endo H,Hotta Ydoi
10.1247/csf.15.67subject
Has Abstractpub_date
1990-02-01 00:00:00pages
67-72issue
1eissn
0386-7196issn
1347-3700journal_volume
15pub_type
杂志文章abstract::CD9 and CD63 belong to a tetramembrane-spanning glycoprotein family called tetraspanin, and are involved in a wide variety of cellular processes, but the structure-function relationship of this family of proteins has yet to be clarified. CD9 associates with diphtheria toxin receptor (DTR), which is identical to the me...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.25.317
更新日期:2000-10-01 00:00:00
abstract::Distribution of peanut agglutinin binding sites was studied histologically with horseradish peroxidase labelled and fluorescein isothiocyanate labelled peanut agglutinin in terms of cell differentiation in rat lymphatic organs, (thymus, spleen, lymph nodes). In this study, alcohol-fixed paraffin-embedded tissue sectio...
journal_title:Cell structure and function
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doi:10.1247/csf.8.29
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abstract::The Acanthamoeba myosin-IA and myosin-IB molecular motors bind to membranes, so they may produce the force to move organelles and membranes along actin filaments. We have determined the rate constants for the actin-activated myosin-I ATPase by pre-steady state kinetic analysis. ATP binds rapidly to myosin-I and dissoc...
journal_title:Cell structure and function
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更新日期:1996-10-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.25.171
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abstract::The epidermal growth factor (EGF)-induced endocytosis of its receptor is an obligatory pathway for the cellular regulation of the EGF-specific receptor (EGF-R). BNER4 is a mouse Balb/3T3 cell line transfected with human EGF-R complementary DNA (cDNA). B4/src-13 and B4/src-24 are BNER4 cells transfected with a viral on...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.19.397
更新日期:1994-12-01 00:00:00
abstract::Newly prepared phosphatidylethanolamine (PE) conjugates of glycosaminoglycans (GAGs) enabled us to immobilize GAGs to solid phase through hydrophobic interaction. Using these compounds, called GAG-PEs, we studied the effects of GAGs immobilized to culture plates coated with various extracellular matrix (ECM) proteins ...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.20.199
更新日期:1995-06-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.24.419
更新日期:1999-10-01 00:00:00
abstract::The relative fibrin-binding, fibrinolytic and fibrinogenolytic properties of single-chain pro-urokinase, an inactive proenzyme form of human urokinase purified from cultured human kidney cells, and urokinase were compared. The affinity of single-chain pro-urokinase for fibrin was much higher than that of urokinase. In...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.10.151
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journal_title:Cell structure and function
pub_type: 杂志文章,评审
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更新日期:2001-12-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.10.95
更新日期:1985-06-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.17.105
更新日期:1992-04-01 00:00:00
abstract::The proto-oncogene c-myc is a multifunctional gene that regulates cell division, cell growth, and apoptosis. Here we report a new function of c-myc: induction of autophagy. Autophagy is a bulk degradation system for intracellular proteins. Autophagy proceeds with characteristic morphologies, which begins with the form...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.28.195
更新日期:2003-06-01 00:00:00
abstract::The putative ionophoretic action of phosphatidic acid or arachidonic acid metabolites for Ca2+ has offered an attractive explanation for stimulation-coupled mobilization of cytoplasmic Ca2+. We have examined the effects of Ca2+ ionophore and long-chain unsaturated fatty acids on the translocation of Ca2+ across the li...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.10.177
更新日期:1985-06-01 00:00:00
abstract::Autophagy is a starvation response in eukaryotes by which the cell delivers cytoplasmic components to the vacuole for degradation, and is mediated by a double membrane structure called the autophagosome. We have previously proposed that the specific combination of COPII like components, including Sec24p, is required f...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.28.49
更新日期:2003-02-01 00:00:00
abstract::Murine tracheal epithelial cell lines, TM01 and TM02-3, were established from a primary culture of tracheal cells of adult transgenic mice bearing a temperature-sensitive simian virus (SV40) large T-antigen gene. Both TM01 and TM02-3 cells, which grew until confluent monolayers were formed, maintained tight contact wi...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.23.119
更新日期:1998-06-01 00:00:00
abstract::The localization of mRNA in neuronal dendrites plays a role in both locally and temporally regulated protein synthesis, which is required for certain forms of synaptic plasticity. RNA granules constitute a dendritic mRNA transport machinery in neurons, which move along microtubules. RNA granules contain densely packed...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
doi:10.1247/csf.30.51
更新日期:2005-01-01 00:00:00
abstract::Developing cultures of dissociated cerebral hemispheres obtained from 18-day-old embryonic rats synthesized, or activated, a myelin-related enzyme, 2',3'-cyclic nucleotide 3'-phosphohydrolase (CNPase). This increase in activity coincided with the onset of myelination. The presence of CNPase in oligodendrocytes and mye...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.10.271
更新日期:1985-09-01 00:00:00
abstract::To elucidate the cytotoxic mechanism of tumor necrosis factor (TNF), we isolated TNF-resistant sublines of L929 cells. As compared with L929 cells, TNF-resistant cells retained similar number and affinity of TNF-binding sites, and showed a similar growth rate. TNF stimulated arachidonate release from L929 cells, while...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.16.333
更新日期:1991-08-01 00:00:00
abstract::The cell cycle of HeLa S3 cells synchronized by hydroxyurea was investigated by flow cytometry. Metachromatic fluorochrome acridine orange was used to strain the DNA and RNA of the cells differentially. Periodic changes in the cellular DNA and RNA contents were observed through five cell cycles. The G1 and S phases of...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.8.303
更新日期:1983-09-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.06025
更新日期:2006-01-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.14.173
更新日期:1989-04-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.14.561
更新日期:1989-10-01 00:00:00
abstract::Addition of hemin to the nuclear reconstitution system of Xenopus interphase egg extract using sperm head chromatin resulted in abnormal pseudonuclei exhibiting flattened membrane patches randomly distributed both on the surface and inside the nuclei. The structures that resembled nuclear pores were observed on these ...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.23.291
更新日期:1998-10-01 00:00:00
abstract::The involvement of actin filaments from the host cell on the process of invasion of trypomastigote forms of Trypanosma cruzi was analyzed in seven different cell lines. Prior incubation of all cell lines with cytochalasin D, under conditions which interfere with actin filaments, markedly inhibited parasite internaliza...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.27.91
更新日期:2002-04-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.19.189
更新日期:1994-08-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
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更新日期:1993-10-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
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更新日期:2002-08-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
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更新日期:1997-08-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
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更新日期:2004-02-01 00:00:00
abstract::It has been shown that in PC12 and its subclone PC12h treatment of the cells with nerve growth factor (NGF) induces a selective decrease in the incorporation of radioactive phosphate into a 100,000-dalton protein, designated in an earlier study as Nsp100, in the subsequent phosphorylation of soluble extracts from cell...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.14.87
更新日期:1989-02-01 00:00:00