Abstract:
:Addition of hemin to the nuclear reconstitution system of Xenopus interphase egg extract using sperm head chromatin resulted in abnormal pseudonuclei exhibiting flattened membrane patches randomly distributed both on the surface and inside the nuclei. The structures that resembled nuclear pores were observed on these flattened membrane patch structures. Although the nucleosome structure was formed as revealed by the micrococcal nuclease digestion, the B-type lamin uptake into the nuclei was inhibited by hemin. Using heminagarose affinity chromatography, we isolated several hemin-binding proteins from fully reconstituted pseudonuclei. Some of the hemin-binding proteins bound concanavalin A (Con A). Comparison of hemin-binding proteins with those isolated from both fractions of supernatant and pellet separated by high speed centrifugation of the egg extract showed that the hemin-binding proteins of pseudonuclei were supplied from both fractions. The uptake of nuclear hemin-binding proteins did not occur in the incompletely reconstituted nuclei resulting from addition of excess sperm chromatin to the system. These results suggest that the hemin-binding proteins participate in the late steps of nuclear reconstitution during formation of the nuclear envelope.
journal_name
Cell Struct Functjournal_title
Cell structure and functionauthors
Nishiyama R,Ikami M,Yamao F,Tsuchida S,Yagura Tdoi
10.1247/csf.23.291subject
Has Abstractpub_date
1998-10-01 00:00:00pages
291-301issue
5eissn
0386-7196issn
1347-3700journal_volume
23pub_type
杂志文章abstract::CD9 and CD63 belong to a tetramembrane-spanning glycoprotein family called tetraspanin, and are involved in a wide variety of cellular processes, but the structure-function relationship of this family of proteins has yet to be clarified. CD9 associates with diphtheria toxin receptor (DTR), which is identical to the me...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.25.317
更新日期:2000-10-01 00:00:00
abstract::Human keratinocytes have been serially cultivated in low (0.015 mM) and high (1.8 mM) calcium containing medium. The calcium concentration of the growth medium significantly influenced the cell growth period in vitro. Cells grown in low calcium medium underwent 35-40 population doublings over 16-17 passages, while cel...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.12.539
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journal_title:Cell structure and function
pub_type: 杂志文章
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更新日期:1989-12-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.9.67
更新日期:1984-03-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章,评审
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更新日期:1991-04-01 00:00:00
abstract::The transcription factor ATF6 is constitutively synthesized as a type II transmembrane protein embedded in the endoplasmic reticulum (ER). When unfolded proteins accumulate in the ER, ATF6 senses such ER stress via an as yet undetermined mechanism and relocates to the Golgi apparatus where it is cleaved by sequential ...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.10010
更新日期:2011-01-01 00:00:00
abstract::Two methods of qualitative analysis of sequence distribution in DNA and protein are presented. The first method is based on the finding that the frequency of occurrence of each nucleotide in a defined sequence with functional significance more or less deviates from uniform distribution. The deviation found in this def...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.13.387
更新日期:1988-10-01 00:00:00
abstract::The purpose of the present study is to analyze the effect of serum or chick embryo extract (CEE) on the neuronal differentiation of the mouse neural crest cells. When the crest cells were cultured in the medium containing serum at low concentration (5% calf serum), neurite outgrowth was observed. The active outgrowth ...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.13.267
更新日期:1988-06-01 00:00:00
abstract::The relative fibrin-binding, fibrinolytic and fibrinogenolytic properties of single-chain pro-urokinase, an inactive proenzyme form of human urokinase purified from cultured human kidney cells, and urokinase were compared. The affinity of single-chain pro-urokinase for fibrin was much higher than that of urokinase. In...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.10.151
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journal_title:Cell structure and function
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更新日期:1998-12-01 00:00:00
abstract::Tritrichomonas foetus strongly agglutinates human erythrocytes, thus suggesting the occurrence of an adhesin associated with its surface. Adherence was observed immediately after mixing of the parasites with erythrocytes and the intensity increased for up to 30 min. Scanning electron microscopy examination of T. foetu...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.21.245
更新日期:1996-08-01 00:00:00
abstract::Epidermal growth factor (EGF) was derivatized at the amino terminus with N-succinimidyl 3-(2-pyridyldithio)propionate and then cross-linked to the cysteinyl residues of alpha 2-macroglobulin (alpha 2M) via disulfide bonds. The EGF-alpha 2M conjugate delivered EGF into dense lysosomal fractions through binding to alpha...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.9.105
更新日期:1984-06-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.25.171
更新日期:2000-06-01 00:00:00
abstract::A protein phosphatase inhibitor, tautomycin induces blebs on the surface of human myeloid leukemia K562 cells within 10 min. In this paper we examined the cytoskeleton of tautomycin-treated cells. In the presence of tautomycin, cells with blebs turned into segmented forms with smooth surfaces after 15 min and into smo...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.18.33
更新日期:1993-02-01 00:00:00
abstract::tsJT60, a temperature-sensitive (ts) G0-mutant cell line from a Fischer rat, grows normally in the exponential growth phase at 34 degrees C and 39.5 degrees C, but when stimulated with fetal bovine serum (FBS), from the G0 phase they reenter the S phase at 34 degrees C but not at 39.5 degrees C. The ts-block was bypas...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.12.421
更新日期:1987-10-01 00:00:00
abstract::The distribution of Ca2+ in dividing cells of the maize root tip was examined by potassium pyroantimonate precipitation and EGTA treatment methods. Ca2+ was found in most of the cell organelles, such as the matrix of the mitochondria, the thylakoid membrane of the proplastid and the Golgi vesicles, and on the plasma m...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.18.389
更新日期:1993-12-01 00:00:00
abstract::Tobacco BY-2 cells were synchronized by an aphidicolin treatment, and their beta-tubulin isoforms and their mRNA were analyzed by Western, Northern and dot blottings. The relative ratio of the beta-tubulin isoforms changed with the progress of cell cycle stage. By Northern blot hybridization of poly(A)+RNAs with a clo...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.16.489
更新日期:1991-12-01 00:00:00
abstract::A monoclonal type M-immunoglobulin (IgM) was generated in mice against a nuclease-urea extract of HeLa metaphase chromosomes. This antibody stains metaphase chromosomes from a variety of mammalian cultured cell types by indirect immunofluorescence. Antibody 12C7 reacts by western transfer technique with histone H1 in ...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.16.323
更新日期:1991-08-01 00:00:00
abstract::Two-dimensional gel electrophoresis revealed two protein spots, a (molecular weight 70,000, pI 4.6) and b (molecular weight 69,000, pI 4.4) in PC-12 cells (rat pheochromocytoma cells). When the cells were induced to differentiate with nerve growth factor, the amount of protein in spot a, and later spot b increased wit...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.12.127
更新日期:1987-02-01 00:00:00
abstract::Tenascin-C is an oligomeric glycoprotein of the extracellular matrix that has been found to have both adhesive and anti-adhesive properties for cells. Recent elucidation of the two major TNC splice variants (320 kDa and 220 kDa) has shed light on the possibility of varying functions of the molecule based on its splici...
journal_title:Cell structure and function
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更新日期:2001-06-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
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更新日期:2007-01-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.10.405
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.15.221
更新日期:1990-08-01 00:00:00
abstract::1. Cofilin is an essential actin-regulating protein widely distributed in all eucaryotes. The structure and function of cofilin are conserved during evolution. 2. Cofilin depolymerizes F-actin in vitro at alkaline pH and severs F-actin in vitro at pH lower than 7.3. Overexpression of cofilin in viable cells induced bu...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
doi:10.1247/csf.21.421
更新日期:1996-10-01 00:00:00
abstract::Mouse embryonic stem (mES) cells can be maintained in undifferentiated state in the presence of a cytokine, leukemia inhibitory factor (LIF). Many investigators found that STAT3 activation is important for the maintenance of pluripotency by LIF. However, the downstream pathways of STAT3 activation are still unknown. T...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.31.53
更新日期:2006-01-01 00:00:00
abstract::One of the proposed roles of sarcoglycan is to stabilize dystrophin glycoprotein complexes in muscle sarcolemma. Involvement in signal transduction has also been proposed and abnormalities in some sarcoglycan genes are known to be responsible for muscular dystrophy. While characterization of sarcoglycans in muscle has...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.06025
更新日期:2006-01-01 00:00:00
abstract::DNA synthesis of WF-1 fibroblasts derived from a patient with Werner's syndrome was stimulated by fetal calf serum and adult human serum but not by various mitogens including epidermal growth factor, platelet-derived growth factor (PDGF), fibroblast growth factor, insulin and 12-O-tetradecanoylphorbol-13-acetate (TPA)...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.13.471
更新日期:1988-12-01 00:00:00
abstract::tsJT60 is a nonlethal temperature-sensitive (ts) mutant of a Fischer rat cell line (3Y1) classified as a G0 mutant; i.e., the ts defect is not expressed within the cell growth cycle but is expressed only between the G0 and S phase. tsJT60 clones transformed with oncogenes such as adenovirus E1A, polyoma large T, polyo...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.15.385
更新日期:1990-12-01 00:00:00
abstract::In adult rat cerebellum, HPC-1/syntaxin 1 is detected at high density on the plasma membrane of the non-synaptic region of parallel fibers in addition to the synaptic terminal membranes and the synaptic vesicles (Koh, S., Yamamoto, A., Inoue, A., Inoue, Y., Akagawa, Y., Kawamura, Y., Kawamoto, Y., and Tashiro, Y. (199...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.21.525
更新日期:1996-12-01 00:00:00
abstract::Microtubules are intrinsically dynamic polymers. Two kinds of dynamic behaviors, dynamic instability and treadmilling, are important for microtubule function in cells. Both dynamic behaviors appear to be tightly regulated, but the cellular molecules and the mechanisms responsible for the regulation remain largely unex...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
doi:10.1247/csf.24.329
更新日期:1999-10-01 00:00:00