Identification of Jmjd1a as a STAT3 downstream gene in mES cells.

Abstract:

:Mouse embryonic stem (mES) cells can be maintained in undifferentiated state in the presence of a cytokine, leukemia inhibitory factor (LIF). Many investigators found that STAT3 activation is important for the maintenance of pluripotency by LIF. However, the downstream pathways of STAT3 activation are still unknown. To look for STAT3-downstream target genes, we performed DD-RT PCR in the presence or absence of LIF. Through further confirmation, we finally selected 8 genes whose expressions were significantly dependent upon the presence of LIF. Among them, Jmjd1a was down-regulated after LIF withdrawal, and it was selected for further investigation. Its expression started to decrease 1 day after the removal of LIF, and disappeared on day 3. It was also shown that STAT3 could bind to the promoter region of Jmjd1a gene. These data demonstrate that Jmjd1a might be a critical signaling molecule underlying the maintenance of pluripotency in mES cells.

journal_name

Cell Struct Funct

authors

Ko SY,Kang HY,Lee HS,Han SY,Hong SH

doi

10.1247/csf.31.53

subject

Has Abstract

pub_date

2006-01-01 00:00:00

pages

53-62

issue

2

eissn

0386-7196

issn

1347-3700

pii

JST.JSTAGE/csf/31.53

journal_volume

31

pub_type

杂志文章
  • Flagellar-associated Protein FAP85 Is a Microtubule Inner Protein That Stabilizes Microtubules.

    abstract::Genomics and proteomics studies in Chlamydomonas have revealed that an axoneme is composed of 200-600 types of proteins, including uncharacterized proteins collectively named flagellar-associated proteins (FAPs). Nine FAPs contain the EF-hand motif; however, they have not yet been well characterized. To find component...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.17023

    authors: Kirima J,Oiwa K

    更新日期:2018-02-16 00:00:00

  • The Position of the GFP Tag on Actin Affects the Filament Formation in Mammalian Cells.

    abstract::Actin, a major component of microfilaments, is involved in various eukaryotic cellular functions. Over the past two decades, actin fused with fluorescent protein has been used as a probe to detect the organization and dynamics of the actin cytoskeleton in living eukaryotic cells. It is generally assumed that the expre...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.17016

    authors: Nagasaki A,T Kijima S,Yumoto T,Imaizumi M,Yamagishi A,Kim H,Nakamura C,Q P Uyeda T

    更新日期:2017-01-01 00:00:00

  • Solo and Keratin Filaments Regulate Epithelial Tubule Morphology.

    abstract::Epithelial tubules, consisting of the epithelial cell sheet with a central lumen, are the basic structure of many organs. Mechanical forces play an important role in epithelial tubulogenesis; however, little is known about the mechanisms controlling the mechanical forces during epithelial tubule morphogenesis. Solo (a...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.18010

    authors: Nishimura R,Kato K,Fujiwara S,Ohashi K,Mizuno K

    更新日期:2018-06-02 00:00:00

  • Ultrastructural studies on the Ca2+ localization in the dividing cells of the maize root tip.

    abstract::The distribution of Ca2+ in dividing cells of the maize root tip was examined by potassium pyroantimonate precipitation and EGTA treatment methods. Ca2+ was found in most of the cell organelles, such as the matrix of the mitochondria, the thylakoid membrane of the proplastid and the Golgi vesicles, and on the plasma m...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.18.389

    authors: Sakai-Wada A,Yagi S

    更新日期:1993-12-01 00:00:00

  • Further investigation of some inhibitors on myogenic differentiation: mechanism of inhibition with HMBA on quail myoblasts transformed with Rous sarcoma virus.

    abstract::To investigate the mechanism of myogenic differentiation, we are using quail myoblast cells (QM cells) transformed with a temperature-sensitive mutant of Rous sarcoma virus (ts-RSV), termed QM-RSV cells. At 35.5 degrees C, a permissive temperature for RSV, QM-RSV cells repeatedly proliferate without differentiation, b...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.21.17

    authors: Hase H,Hirayama E,Kim J

    更新日期:1996-02-01 00:00:00

  • Ganglioside composition of astrocytes.

    abstract::Short-term and long-term (greater than 7 months) cultured astrocytes from 14-day-old rat brain were analyzed for ganglioside content. Analysis of the extracted gangliosides by HPTLC revealed that ganglioside GM1 was absent in 35 days and 235 days cultured astrocytes, and that the predominant ganglioside was GM3, showi...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.14.561

    authors: Asou H,Hirano S,Uyemura K

    更新日期:1989-10-01 00:00:00

  • Tumor-promoting, phorbol ester-induced phosphorylation of cell-surface transferrin receptors in human erythroleukemia cells.

    abstract::When human erythroleukemia cells (K562) were exposed to phorbol-12-myristate 13-acetate (PMA), phosphorylation of transferrin receptors was enhanced 5-fold with 10(-7) M PMA and 7-fold with 10(-6) M PMA, but not with 4 alpha-phorbol (5 X 10(-7) M). Stimulation took place in serine residues in the cytoplasmic domain of...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.10.95

    authors: Kohno H,Taketani S,Tokunaga R

    更新日期:1985-06-01 00:00:00

  • Regulatory role of extracellular matrix components in expression of matrix metalloproteinases in cultured hepatic stellate cells.

    abstract::Hepatic stellate cells (HSCs) were changed in their morphology, proliferative activity, and functions by culturing on type I collagen gel, as compared to the culture on polystyrene surface. HSCs have been found to produce extracellular matrix components and matrix metalloproteinases (MMPs). In this study, we have asse...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.24.255

    authors: Li YL,Sato M,Kojima N,Miura M,Senoo H

    更新日期:1999-10-01 00:00:00

  • Atg101: Not Just an Accessory Subunit in the Autophagy-initiation Complex.

    abstract::The Saccharomyces cerevisiae autophagy-initiation complex, Atg1 kinase complex, consists of Atg1, Atg13, Atg17, Atg29, and Atg31, while the corresponding complex in most other eukaryotes, including mammals, is composed of ULK1 (or ULK2), Atg13, FIP200 (also known as RB1CC1), and Atg101. ULKs are homologs of Atg1, and ...

    journal_title:Cell structure and function

    pub_type: 杂志文章,评审

    doi:10.1247/csf.15013

    authors: Noda NN,Mizushima N

    更新日期:2016-01-01 00:00:00

  • Inhibition of cell proliferation by a unique lysophosphatidic acid, PHYLPA, isolated from Physarum polycephalum: signaling events of antiproliferative action by PHYLPA.

    abstract::The unique Physarum lysophosphatidic acid, PHYLPA, having a cyclopropane in the fatty acid moiety and a cyclic phosphate at C-2 and C-3 positions of the glycerol, inhibited proliferation of human fibroblast cells, TIG-3 and TIG-7, which were cultured in a chemically defined (serum-free) medium. The cells at S- and M-p...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.18.363

    authors: Murakami-Murofushi K,Kaji K,Kano K,Fukuda M,Shioda M,Murofushi H

    更新日期:1993-10-01 00:00:00

  • Ca2+ translocation across liposomal membranes enhanced by unsaturated long-chain fatty acids.

    abstract::The putative ionophoretic action of phosphatidic acid or arachidonic acid metabolites for Ca2+ has offered an attractive explanation for stimulation-coupled mobilization of cytoplasmic Ca2+. We have examined the effects of Ca2+ ionophore and long-chain unsaturated fatty acids on the translocation of Ca2+ across the li...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.10.177

    authors: Utsumi K,Nobori K,Miyahara M

    更新日期:1985-06-01 00:00:00

  • Intracellular transport of albumin through the secretory apparatus of rat liver parenchymal cells. An immunocytochemical study.

    abstract::The fine structural localization of albumin in rat liver parenchymal cells was determined by an improved immunocytochemical method and serial sectioning. Albumin in the secretory apparatus of the parenchymal cells was present in segments of the rough endoplasmic reticulum, interrupted with negative segments, in transp...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.12.251

    authors: Yokota S,Fahimi DH

    更新日期:1987-06-01 00:00:00

  • The molecular mechanism of targeted vesicle transport in cytokinesis.

    abstract::Recent studies have demonstrated that vesicle transport to cleavage furrow is indispensable for cytokinesis. Some animal and plant cells form distinct structures during cell division known as central spindle and phragmoplast, respectively. Several essential factors involved in the vesicle transport have been isolated ...

    journal_title:Cell structure and function

    pub_type: 杂志文章,评审

    doi:10.1247/csf.26.567

    authors: Edamatsu M

    更新日期:2001-12-01 00:00:00

  • Co-localization of urokinase and its receptor on established human umbilical vein endothelial cell.

    abstract::Vascular endothelial cells possess antithrombotic properties, which are determined by the balance between plasminogen activators (PAs) and PA inhibitors (PAls). A cell line, TKM-33, has been established and cloned from human umbilical vein endothelial cells, was previously reported to produce a large amount of urokina...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.24.71

    authors: Ueshima S,Matsumoto H,Izaki S,Mitsui Y,Fukao H,Okada K,Matsuo O

    更新日期:1999-04-01 00:00:00

  • Isolation and characterization of nuclei from rice embryos.

    abstract::A method has been developed to isolate pure preparations of nuclei in high yield from commercially available viable rice embryos (germ), employing extraction with buffer solution containing glycerol (without detergent) and polyamine, followed by centrifugation on a 30% Percoll cushion. The intactness of the isolated n...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.17.87

    authors: Yamaguchi J,Lim PY,Aratani K,Akazawa T

    更新日期:1992-04-01 00:00:00

  • Rough surfaced smooth endoplasmic reticulum in rat and mouse cerebellar Purkinje cells visualized by quick-freezing techniques.

    abstract::The in vivo structure of the smooth endoplasmic reticulum (ER) was visualized in rat and mouse cerebellar Purkinje cells by using quick-freezing techniques followed by freeze-substitution for ultrathin-sectioning or freeze-fracturing and deep-etching for replicas. High magnification electron microscopy of the ultrathi...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.23.373

    authors: Kanaseki T,Ikeuchi Y,Tashiro Y

    更新日期:1998-12-01 00:00:00

  • Transcriptional activation of mouse major satellite regions during neuronal differentiation.

    abstract::Recent studies have revealed various biological functions for repetitive sequences, which make up about half of the human genome. One such sequence, major satellites, which are tandem repetitive sequences adjacent to the centromere, have been shown to be a kinetochore component that plays a role in the formation and f...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.12009

    authors: Kishi Y,Kondo S,Gotoh Y

    更新日期:2012-01-01 00:00:00

  • Population-dependent requirements of vitamin B12 and metabolically related substances of several mouse cell types in serum-free, albumin-fortified medium.

    abstract::Mouse FM3A cells propagated well in serum-free medium containing 0.5% serum albumin and 1 microgram of insulin/ml. The vitamin B12 (B12) requirement of the cells depended on the population density. This requirement disappeared when a sufficiently large cell population was present. A combination of 1-100 ng of B12/ml a...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.11.9

    authors: Matsuya Y,Yamane I

    更新日期:1986-03-01 00:00:00

  • MiR-187-3p Enhances Propranolol Sensitivity of Hemangioma Stem Cells.

    abstract::Infantile hemangioma is the most common soft tissue tumors in childhood. In clinic, propranolol is widely used for infantile hemangioma therapy. However, some of the infantile hemangioma patients display resistance to propranolol treatment. Previous studies show that miR-187-3p is inhibited in hepatocellular carcinoma...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.18041

    authors: Liu C,Zhao Z,Ji Z,Jiang Y,Zheng J

    更新日期:2019-03-20 00:00:00

  • Immunocytochemical analysis of peptide hormone processing: importance of the positively charged N-terminal domain of signal peptide in correct ER targeting in yeast cells.

    abstract::We used a morphological approach to determine the topogenic role of the signal peptide in mediating the ER translocation of yeast prepro-alpha-factor. In prepro-alpha-factor-somatostatin hybrids, changes in the N-terminal amino acid sequence from wild-type NH2-Met-Arg-Phe (MRF) to NH2-Met-Phe-Lys (MFK) caused a subtle...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.22.365

    authors: Cheong KH,Park SD,Kim J,Hong SH

    更新日期:1997-06-01 00:00:00

  • c-myc induces autophagy in rat 3Y1 fibroblast cells.

    abstract::The proto-oncogene c-myc is a multifunctional gene that regulates cell division, cell growth, and apoptosis. Here we report a new function of c-myc: induction of autophagy. Autophagy is a bulk degradation system for intracellular proteins. Autophagy proceeds with characteristic morphologies, which begins with the form...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.28.195

    authors: Tsuneoka M,Umata T,Kimura H,Koda Y,Nakajima M,Kosai K,Takahashi T,Takahashi Y,Yamamoto A

    更新日期:2003-06-01 00:00:00

  • Wheat germ agglutinin, concanavalin A, and lens culinalis agglutinin block the inhibitory effect of nerve growth factor on cell-free phosphorylation of Nsp100 in PC12h cells.

    abstract::It has been shown that in PC12 and its subclone PC12h treatment of the cells with nerve growth factor (NGF) induces a selective decrease in the incorporation of radioactive phosphate into a 100,000-dalton protein, designated in an earlier study as Nsp100, in the subsequent phosphorylation of soluble extracts from cell...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.14.87

    authors: Hashimoto S,Hagino A

    更新日期:1989-02-01 00:00:00

  • An altered nuclear migration into the daughter bud is induced by the cyclin A1-mediated Cdc28 kinase through an aberrant spindle movement in Saccharomyces cerevisiae.

    abstract::A strain of Saccharomyces cerevisiae that contains an integrated copy of a Xenopus cyclin A1 gene under the control of the GAL1 promoter has been constructed. On inducing expression of cyclin A1, the nuclear migration that occurs prior to division becomes aberrant. Instead of migrating to the neck between the mother c...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.22.465

    authors: Sikder H,Funakoshi M,Nishimoto T,Kobayashi H

    更新日期:1997-08-01 00:00:00

  • Tenascin-C splice variant adhesive/anti-adhesive effects on chondrosarcoma cell attachment to fibronectin.

    abstract::Tenascin-C is an oligomeric glycoprotein of the extracellular matrix that has been found to have both adhesive and anti-adhesive properties for cells. Recent elucidation of the two major TNC splice variants (320 kDa and 220 kDa) has shed light on the possibility of varying functions of the molecule based on its splici...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.26.179

    authors: Ghert MA,Qi WN,Erickson HP,Block JA,Scully SP

    更新日期:2001-06-01 00:00:00

  • Different effects of all-trans-retinoic acid on phorbol ester-stimulated and phytohemagglutinin-stimulated interleukin-2 expression in human T-cell lymphoma HUT-78 cells.

    abstract::In view of the importance of vitamin A in the human immune system and the central role of interleukin-2 (IL-2) in the proliferation of T-lymphocytes, we examined the effects of all-trans-retinoic acid (ATRA) on the protein and gene expression of IL-2 in the human T-cell line HUT-78 when stimulated with either 12-O-tet...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.07020

    authors: Goto S,Okada N,Kaneko A,Isemura M

    更新日期:2008-01-01 00:00:00

  • High voltage electric pulses efficiently induce fusion of cells in monolayer culture.

    abstract::High voltage electric pulses, 1000 V/cm, were found to induce cell fusion efficiently when delivered to cells growing in a monolayer culture. The maximum yield of fused cells was about 30% of cells remaining after treatment. Colonies of interspecies hybrid cells between mouse and others also appeared with a frequency ...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.14.173

    authors: Ishizaki K,Chang HR,Eguchi T,Ikenaga M

    更新日期:1989-04-01 00:00:00

  • Heavy ion microbeam irradiation induces ultrastructural changes in isolated single fibers of skeletal muscle.

    abstract::The effects of heavy ion microbeams on muscle fibers isolated from mouse skeletal muscles were examined by electron microscopy. The plasma membranes of heavy ion beam-irradiated areas of muscle fibers showed irregular protrusions and invaginations. In the cytoplasm, an irregular distribution of microfilaments was foun...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.06038

    authors: Hino M,Wada S,Tajika Y,Morimura Y,Hamada N,Funayama T,Sakashita T,Kakizaki T,Kobayashi Y,Yorifuji H

    更新日期:2007-01-01 00:00:00

  • Hemocytes release phenoloxidase upon contact reaction, an allogeneic interaction, in the ascidian Halocynthia roretzi.

    abstract::Contact reaction is the morphological expression of allogeneic recognition by hemocytes in the ascidian, Halocynthia roretzi; namely they undergo an exocytotic burst upon contact with non-self (allogeneic) hemocytes in vitro. We have found that contact reaction is accompanied by a burst of cyanide-insensitive oxygen c...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.20.81

    authors: Akita N,Hoshi M

    更新日期:1995-02-01 00:00:00

  • Both low and high concentrations of staurosporine induce G1 arrest through down-regulation of cyclin E and cdk2 expression.

    abstract::Staurosporine has been reported to cause arrest of cells in G1 phase at low concentration and in G2 phase at high concentration. This raises the question of why the effects of staurosporine on the cell cycle depend on the applied concentration. In order to verify these multiple functions of staurosporine in Meth-A cel...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.24.457

    authors: Zong ZP,Fujikawa-Yamamoto K,Li AL,Yamaguchi N,Chang YG,Murakami M,Odashima S,Ishikawa Y

    更新日期:1999-12-01 00:00:00

  • The relation between length of the cell cycle duration and RNA content in HeLa S3 cells.

    abstract::The cell cycle of HeLa S3 cells synchronized by hydroxyurea was investigated by flow cytometry. Metachromatic fluorochrome acridine orange was used to strain the DNA and RNA of the cells differentially. Periodic changes in the cellular DNA and RNA contents were observed through five cell cycles. The G1 and S phases of...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.8.303

    authors: Fujikawa-Yamamoto K

    更新日期:1983-09-01 00:00:00