Abstract:
:A method has been developed to isolate pure preparations of nuclei in high yield from commercially available viable rice embryos (germ), employing extraction with buffer solution containing glycerol (without detergent) and polyamine, followed by centrifugation on a 30% Percoll cushion. The intactness of the isolated nuclei was confirmed by light microscopy as well as electron microscopy. The protein profiles of both whole nuclei and nuclear extracts obtained by SDS-PAGE, organellar marker enzyme activities, DNA and RNA analyses, and in vitro RNA synthesis, all indicate that the highly purified nuclei are isolated from rice embryos.
journal_name
Cell Struct Functjournal_title
Cell structure and functionauthors
Yamaguchi J,Lim PY,Aratani K,Akazawa Tdoi
10.1247/csf.17.87keywords:
subject
Has Abstractpub_date
1992-04-01 00:00:00pages
87-92issue
2eissn
0386-7196issn
1347-3700journal_volume
17pub_type
杂志文章abstract::The transcription factor ATF6 is constitutively synthesized as a type II transmembrane protein embedded in the endoplasmic reticulum (ER). When unfolded proteins accumulate in the ER, ATF6 senses such ER stress via an as yet undetermined mechanism and relocates to the Golgi apparatus where it is cleaved by sequential ...
journal_title:Cell structure and function
pub_type: 杂志文章
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journal_title:Cell structure and function
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journal_title:Cell structure and function
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journal_title:Cell structure and function
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journal_title:Cell structure and function
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journal_title:Cell structure and function
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.20.199
更新日期:1995-06-01 00:00:00
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journal_title:Cell structure and function
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journal_title:Cell structure and function
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journal_title:Cell structure and function
pub_type: 杂志文章
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journal_title:Cell structure and function
pub_type: 杂志文章
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更新日期:1994-02-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
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更新日期:1997-04-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.24.255
更新日期:1999-10-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
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更新日期:2006-01-01 00:00:00
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pub_type: 杂志文章
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journal_title:Cell structure and function
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journal_title:Cell structure and function
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journal_title:Cell structure and function
pub_type: 杂志文章
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更新日期:2001-10-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.16.489
更新日期:1991-12-01 00:00:00
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更新日期:1997-08-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章,评审
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更新日期:2000-08-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.8.303
更新日期:1983-09-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
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journal_title:Cell structure and function
pub_type: 杂志文章
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更新日期:1993-02-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.12.273
更新日期:1987-06-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章,评审
doi:10.1247/csf.22.219
更新日期:1997-02-01 00:00:00