Abstract:
:We earlier discovered a novel 40-kDa protein (hsp40) induced by heat shock and other stresses in mammalian and avian cells. In this report, we purified the hsp40 in HeLa cells, using modified two-dimensional gel electrophoresis, and determined the amino terminal amino acid sequence of this protein. The hsp40 is homologous to DnaJ, an Escherichia coli heat-shock protein, as well as to DnaJ-homologous proteins in yeast such as SCJ1, Sec63/Np11, YDJ1 and SIS1. Indirect immunofluorescence staining using an anti-hsp40 polyclonal antibody demonstrated that hsp40 was localized faintly throughout the cell in non-heat-shocked cells and was accumulated in nuclei and nucleoli in heat-shocked cells. The intracellular localization of hsp40 was very similar to that of hsp70, suggesting that these two hsps colocalize in heat-shocked HeLa cells.
journal_name
Cell Struct Functjournal_title
Cell structure and functionauthors
Hattori H,Liu YC,Tohnai I,Ueda M,Kaneda T,Kobayashi T,Tanabe K,Ohtsuka Kdoi
10.1247/csf.17.77keywords:
subject
Has Abstractpub_date
1992-02-01 00:00:00pages
77-86issue
1eissn
0386-7196issn
1347-3700journal_volume
17pub_type
杂志文章abstract::Ran is a nuclear Ras-like GTPase that is required for various nuclear events including the bi-directional transport of proteins and ribonucleoproteins through the nuclear pore complex, spindle formation, and reassembly of the nuclear envelope. One of the key regulators of Ran is RanGAP1, a Ran specific GTPase activati...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.30.69
更新日期:2005-01-01 00:00:00
abstract::We investigated the assembly of the first sarcomeres in chicken embryos by confocal microscopy of immunofluorescently stained whole mount rudiments of early chicken hearts isolated around the onset of beating. In embryos with merely 9 somites, myomesin was found to be present in a cross striated pattern, indicating th...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.22.139
更新日期:1997-02-01 00:00:00
abstract::A strain of Saccharomyces cerevisiae that contains an integrated copy of a Xenopus cyclin A1 gene under the control of the GAL1 promoter has been constructed. On inducing expression of cyclin A1, the nuclear migration that occurs prior to division becomes aberrant. Instead of migrating to the neck between the mother c...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.22.465
更新日期:1997-08-01 00:00:00
abstract::The Saccharomyces cerevisiae autophagy-initiation complex, Atg1 kinase complex, consists of Atg1, Atg13, Atg17, Atg29, and Atg31, while the corresponding complex in most other eukaryotes, including mammals, is composed of ULK1 (or ULK2), Atg13, FIP200 (also known as RB1CC1), and Atg101. ULKs are homologs of Atg1, and ...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
doi:10.1247/csf.15013
更新日期:2016-01-01 00:00:00
abstract::To elucidate the cytotoxic mechanism of tumor necrosis factor (TNF), we isolated TNF-resistant sublines of L929 cells. As compared with L929 cells, TNF-resistant cells retained similar number and affinity of TNF-binding sites, and showed a similar growth rate. TNF stimulated arachidonate release from L929 cells, while...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.16.333
更新日期:1991-08-01 00:00:00
abstract::In yeast Saccharomyces cerevisiae cells, some aberrant multimembrane-spanning proteins are not transported to the cell surface but form and are accumulated in endoplasmic reticulum (ER)-derived subcompartments, known as the ER-associated compartments (ERACs), which are observed as puncta under fluorescence microscopy....
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.19023
更新日期:2019-11-23 00:00:00
abstract::Under unfavorable conditions for growth, haploid myxoamoebae of Physarum polycephalum retracted their pseudopodia and changed their cell shape into disk-like form, after which they constructed the cell walls to form microcysts. These morphological changes of haploid cells were associated with changes in intracellular ...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.17.301
更新日期:1992-10-01 00:00:00
abstract::In the present study, we compared the abilities of ricin and diphtheria toxin to induce apoptosis in Vero cells. The cytolysis and DNA fragmentation by ricin paralleled its protein synthesis inhibitory activity. However, unlike ricin, diphtheria toxin could induce neither cytolysis nor DNA fragmentation in Vero cells ...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.26.279
更新日期:2001-10-01 00:00:00
abstract::Mouse embryonic stem (mES) cells can be maintained in undifferentiated state in the presence of a cytokine, leukemia inhibitory factor (LIF). Many investigators found that STAT3 activation is important for the maintenance of pluripotency by LIF. However, the downstream pathways of STAT3 activation are still unknown. T...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.31.53
更新日期:2006-01-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.06013
更新日期:2006-01-01 00:00:00
abstract::Interleukin 2 (IL-2) initiated proliferation of the cells of T-enriched population stimulated with non-mitogenic dose of concanavalin A (Con A), whereas it could not induce proliferation of the cells treated with non-mitogenic lectin wheat germ agglutinin (WGA). Use of monoclonal antibody (MAb) directed against interl...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.14.193
更新日期:1989-04-01 00:00:00
abstract::We have previously shown that mouse L.P3 cells secrete fibronectin and a novel protein, gelatin-non-binding and heparin-binding cell-adhesive protein (GNCP). Here, we screened and characterized cell-adhesive proteins in the conditioned media (CM's) of a series of cell lines growing in a protein- and lipid-free synthet...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.18.61
更新日期:1993-02-01 00:00:00
abstract::Infantile hemangioma is the most common soft tissue tumors in childhood. In clinic, propranolol is widely used for infantile hemangioma therapy. However, some of the infantile hemangioma patients display resistance to propranolol treatment. Previous studies show that miR-187-3p is inhibited in hepatocellular carcinoma...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.18041
更新日期:2019-03-20 00:00:00
abstract::The distribution of Ca2+ in dividing cells of the maize root tip was examined by potassium pyroantimonate precipitation and EGTA treatment methods. Ca2+ was found in most of the cell organelles, such as the matrix of the mitochondria, the thylakoid membrane of the proplastid and the Golgi vesicles, and on the plasma m...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.18.389
更新日期:1993-12-01 00:00:00
abstract::Mechanistic target of rapamycin complex 1 (mTORC1) plays a pivotal role in controlling cell growth and metabolism in response to nutrients and growth factors. The activity of mTORC1 is dually regulated by amino acids and growth factor signaling, and amino acid-dependent mTORC1 activity is regulated by mTORC1 interacti...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.20018
更新日期:2020-01-01 00:00:00
abstract::The ETS family of transcription factors is defined by a conserved DNA-binding ETS domain that forms a winged helix-turn-helix structural motif. This family of transcription factors is involved in a diverse array of biological functions including cellular growth and differentiation, as well as organ development. Among ...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
doi:10.1247/csf.26.19
更新日期:2001-02-01 00:00:00
abstract::The effects of heavy ion microbeams on muscle fibers isolated from mouse skeletal muscles were examined by electron microscopy. The plasma membranes of heavy ion beam-irradiated areas of muscle fibers showed irregular protrusions and invaginations. In the cytoplasm, an irregular distribution of microfilaments was foun...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.06038
更新日期:2007-01-01 00:00:00
abstract::Hepatic stellate cells (HSCs), a mesenchymal cell type in hepatic parenchyma, have unique features with respect to their cellular origin, morphology, and function. Normal, quiescent HSCs function as major vitamin A-storing cells containing over 80% of total vitamin A in the body to maintain vitamin A homeostasis. HSCs...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
doi:10.1247/csf.28.105
更新日期:2003-04-01 00:00:00
abstract::A voltage-dependent and Ca2(+)-activated cation channel recently found in the vacuolar membrane of the yeast Saccharomyces cerevisiae was incorporated into planar lipid bilayers and further characterized in macroscopic and single channel levels. Single channel conductances for various cations were in the order: NH4+ g...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.14.659
更新日期:1989-12-01 00:00:00
abstract::The Acanthamoeba myosin-IA and myosin-IB molecular motors bind to membranes, so they may produce the force to move organelles and membranes along actin filaments. We have determined the rate constants for the actin-activated myosin-I ATPase by pre-steady state kinetic analysis. ATP binds rapidly to myosin-I and dissoc...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
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更新日期:1996-10-01 00:00:00
abstract::Genomics and proteomics studies in Chlamydomonas have revealed that an axoneme is composed of 200-600 types of proteins, including uncharacterized proteins collectively named flagellar-associated proteins (FAPs). Nine FAPs contain the EF-hand motif; however, they have not yet been well characterized. To find component...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.17023
更新日期:2018-02-16 00:00:00
abstract::Primary hepatocytes were cultured on collagen gel in serum-free, alpha-modified Eagle's minimum essential medium containing 0.1 microM insulin, 0.1 microM dexamethasone, 10 mM pyruvate and supplements such as glucagon, epinephrine or growth hormone. The activities of alkaline phosphatase, 5'-nucleotidase and gamma-glu...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.18.73
更新日期:1993-02-01 00:00:00
abstract::One of the proposed roles of sarcoglycan is to stabilize dystrophin glycoprotein complexes in muscle sarcolemma. Involvement in signal transduction has also been proposed and abnormalities in some sarcoglycan genes are known to be responsible for muscular dystrophy. While characterization of sarcoglycans in muscle has...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.06025
更新日期:2006-01-01 00:00:00
abstract::tsJT60 is a nonlethal temperature-sensitive (ts) mutant of a Fischer rat cell line (3Y1) classified as a G0 mutant; i.e., the ts defect is not expressed within the cell growth cycle but is expressed only between the G0 and S phase. tsJT60 clones transformed with oncogenes such as adenovirus E1A, polyoma large T, polyo...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.15.385
更新日期:1990-12-01 00:00:00
abstract::Synapsin I, one of the major synaptic proteins, is thought to associate with synaptic vesicles and to play a regulatory role in neurotransmitter release. In mature neurons, it is concentrated almost exclusively in presynaptic nerve endings. Here, we studied the subcellular localization of synapsin I during the develop...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.15.329
更新日期:1990-12-01 00:00:00
abstract::Murine tracheal epithelial cell lines, TM01 and TM02-3, were established from a primary culture of tracheal cells of adult transgenic mice bearing a temperature-sensitive simian virus (SV40) large T-antigen gene. Both TM01 and TM02-3 cells, which grew until confluent monolayers were formed, maintained tight contact wi...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.23.119
更新日期:1998-06-01 00:00:00
abstract::Newly prepared phosphatidylethanolamine (PE) conjugates of glycosaminoglycans (GAGs) enabled us to immobilize GAGs to solid phase through hydrophobic interaction. Using these compounds, called GAG-PEs, we studied the effects of GAGs immobilized to culture plates coated with various extracellular matrix (ECM) proteins ...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.20.199
更新日期:1995-06-01 00:00:00
abstract::DNA-dependent protein kinase (DNA-PK), consisting of the 470-kDa catalytic component (DNA-PKcs) and the DNA-binding regulatory component Ku protein (p70/p80), catalyzes phosphorylation of a variety of DNA replication/transcription/repair factors in the presence of double-stranded DNA. In the resting states of human pe...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.22.585
更新日期:1997-12-01 00:00:00
abstract::Stimulation of polymorphonuclear leukocytes (PMN) by tetravalent concanavalin A (alpha-ConA) induces membrane depolarization preceding the onset of superoxide anion (O2-) production. Both divalent and monovalent ConA analogues were studied to evaluate the role of valence. Monovalent ConA (m-ConA) was inactive in stimu...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.11.199
更新日期:1986-06-01 00:00:00
abstract::Upon tryptic digestion of synaptosomes, ATPase activities decreased in the order of Cl(-)-ATPase greater than or equal to Na+,K+-ATPase greater than anion-insensitive Mg2+-ATPase. Upon synaptosome treatment with hypotonic solution, Cl(-)-ATPase or anion-insensitive Mg2+-ATPase was slightly inactivated, while Na+,K+-AT...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.13.105
更新日期:1988-04-01 00:00:00