p18/Lamtor1-mTORC1 Signaling Controls Development of Mucin-producing Goblet Cells in the Intestine.

abstract：:A protein phosphatase inhibitor, tautomycin induces blebs on the surface of human myeloid leukemia K562 cells within 10 min. In this paper we examined the cytoskeleton of tautomycin-treated cells. In the presence of tautomycin, cells with blebs turned into segmented forms with smooth surfaces after 15 min and into smo...

journal_title：Cell structure and function

authors： Kurisaki T,Magae J,Nagai K,Hirata A,Kusaka I,Shinji H,Morikawa M,Yoshida T,Isono K,Yamasaki M

更新日期：1993-02-01 00:00:00

abstract：:To study the effects of surface materials of cells on the behavior of other neighboring cells in a crowded culture, confluent sheets of rat 3Y1 fibroblasts were fixed and then 3Y1 cells were seeded on to them. Among confluent sheets unfixed, fixed with formalin and fixed with ethanol and an empty plastic dish surface,...

journal_title：Cell structure and function

authors： Okuda A,Sasaki M,Kimura G

更新日期：1990-10-01 00:00:00

abstract：:Developing cultures of dissociated cerebral hemispheres obtained from 18-day-old embryonic rats synthesized, or activated, a myelin-related enzyme, 2',3'-cyclic nucleotide 3'-phosphohydrolase (CNPase). This increase in activity coincided with the onset of myelination. The presence of CNPase in oligodendrocytes and mye...

journal_title：Cell structure and function

authors： Hirano S,Iwasaki-Mutou N,Asou H,Ogawa Y

更新日期：1985-09-01 00:00:00

abstract：:All trans retinoic acid (ATRA), a differentiation inducer for human myeloid NB4 cells, induced accumulation of lipid droplet as determined by positivity of Nile Red and Oil Red O in this cell line. Granulocyte colony-stimulating factor (G-CSF), although not having detectable effect by itself, exerted the additive effe...

journal_title：Cell structure and function

authors： Inazawa Y,Nakatsu M,Yasugi E,Saeki K,Yuo A

更新日期：2003-10-01 00:00:00

abstract：:A monoclonal type M-immunoglobulin (IgM) was generated in mice against a nuclease-urea extract of HeLa metaphase chromosomes. This antibody stains metaphase chromosomes from a variety of mammalian cultured cell types by indirect immunofluorescence. Antibody 12C7 reacts by western transfer technique with histone H1 in ...

journal_title：Cell structure and function

authors： Ortiz M,Bejarano LA,Rendón MC,Martin G,Brinkley BR,Valdivia MM

更新日期：1991-08-01 00:00:00

abstract：:The amount of intact 40S rRNA precursor was followed by Northern hybridization in the course of the early embryogenesis of wild-type Xenopus laevis and its anucleolate mutant. The total amount of 40S rRNA precursor did not alter appreciably until the midblastula stage, decreased at the late blastula stage, and then in...

journal_title：Cell structure and function

authors： Furuno N,Shiokawa K,Kobayashi H

更新日期：1988-04-01 00:00:00

abstract：:The Acanthamoeba myosin-IA and myosin-IB molecular motors bind to membranes, so they may produce the force to move organelles and membranes along actin filaments. We have determined the rate constants for the actin-activated myosin-I ATPase by pre-steady state kinetic analysis. ATP binds rapidly to myosin-I and dissoc...

journal_title：Cell structure and function

authors： Pollard TD,Ostap EM

更新日期：1996-10-01 00:00:00

abstract：:tsJT60 is a nonlethal temperature-sensitive (ts) mutant of a Fischer rat cell line (3Y1) classified as a G0 mutant; i.e., the ts defect is not expressed within the cell growth cycle but is expressed only between the G0 and S phase. tsJT60 clones transformed with oncogenes such as adenovirus E1A, polyoma large T, polyo...

journal_title：Cell structure and function

authors： Tai YY,Ninomiya-Tsuji J,Furuoku K,Ogawa N,Ishibashi S,Shiroki K,Segawa K,Tsuchida N,Shibuya M,Ide T

更新日期：1990-12-01 00:00:00

abstract：:Addition of hemin to the nuclear reconstitution system of Xenopus interphase egg extract using sperm head chromatin resulted in abnormal pseudonuclei exhibiting flattened membrane patches randomly distributed both on the surface and inside the nuclei. The structures that resembled nuclear pores were observed on these ...

journal_title：Cell structure and function

authors： Nishiyama R,Ikami M,Yamao F,Tsuchida S,Yagura T

更新日期：1998-10-01 00:00:00

abstract：:Contact reaction is the morphological expression of allogeneic recognition by hemocytes in the ascidian, Halocynthia roretzi; namely they undergo an exocytotic burst upon contact with non-self (allogeneic) hemocytes in vitro. We have found that contact reaction is accompanied by a burst of cyanide-insensitive oxygen c...

journal_title：Cell structure and function

authors： Akita N,Hoshi M

更新日期：1995-02-01 00:00:00

abstract：:Immunoblots of proteins extracted from the skin of a small viviparous fish (Xiphophorus) showed that a monoclonal antibody against human urokinase recognizes multiple molecular weight species of antigens. The immunoaffinity-purified antigens had serine-protease activity for the hydrolysis of a chromogenic substrate an...

journal_title：Cell structure and function

authors： Takahashi K,Wakamatsu Y,Ozato K,Wakayama Y

更新日期：1987-02-01 00:00:00

abstract：:The precise control of spindle microtubule (MT) dynamics is essential for chromosome capture and alignment. Kif18A/kinesin-8, an essential regulator of kinetochore MT dynamics, accumulates at its plus-ends in metaphase but not prometaphase cells. The underlying mechanism of time-dependent and kinetochore MT-specific p...

journal_title：Cell structure and function

authors： Masuda N,Shimodaira T,Shiu SJ,Tokai-Nishizumi N,Yamamoto T,Ohsugi M

更新日期：2011-01-01 00:00:00

abstract：:We used a morphological approach to determine the topogenic role of the signal peptide in mediating the ER translocation of yeast prepro-alpha-factor. In prepro-alpha-factor-somatostatin hybrids, changes in the N-terminal amino acid sequence from wild-type NH2-Met-Arg-Phe (MRF) to NH2-Met-Phe-Lys (MFK) caused a subtle...

journal_title：Cell structure and function

authors： Cheong KH,Park SD,Kim J,Hong SH

更新日期：1997-06-01 00:00:00

abstract：:Under unfavorable conditions for growth, haploid myxoamoebae of Physarum polycephalum retracted their pseudopodia and changed their cell shape into disk-like form, after which they constructed the cell walls to form microcysts. These morphological changes of haploid cells were associated with changes in intracellular ...

journal_title：Cell structure and function

authors： Shimada Y,Kasakura T,Yokota M,Miyata Y,Murofushi H,Sakai H,Yahara I,Murakami-Murofushi K

更新日期：1992-10-01 00:00:00

abstract：:Immunohistochemical analyses of estradiol, progesterone and progesterone receptor were carried out in human salivary gland and salivary adenoid cystic carcinoma. Immunoreactivity to estradiol and progesterone was found in cytoplasm of the cells of the excretory duct system within normal salivary glands, whereas the pr...

journal_title：Cell structure and function

authors： Ozono S,Onozuka M,Sato K,Ito Y

更新日期：1992-06-01 00:00:00

abstract：:The cell cycle of HeLa S3 cells synchronized by hydroxyurea was investigated by flow cytometry. Metachromatic fluorochrome acridine orange was used to strain the DNA and RNA of the cells differentially. Periodic changes in the cellular DNA and RNA contents were observed through five cell cycles. The G1 and S phases of...

journal_title：Cell structure and function

authors： Fujikawa-Yamamoto K

更新日期：1983-09-01 00:00:00

abstract：:One of the proposed roles of sarcoglycan is to stabilize dystrophin glycoprotein complexes in muscle sarcolemma. Involvement in signal transduction has also been proposed and abnormalities in some sarcoglycan genes are known to be responsible for muscular dystrophy. While characterization of sarcoglycans in muscle has...

journal_title：Cell structure and function

authors： Hashimoto R,Yamaguchi M

更新日期：2006-01-01 00:00:00

abstract：:The purpose of the present study is to analyze the effect of serum or chick embryo extract (CEE) on the neuronal differentiation of the mouse neural crest cells. When the crest cells were cultured in the medium containing serum at low concentration (5% calf serum), neurite outgrowth was observed. The active outgrowth ...

journal_title：Cell structure and function

authors： Ito K,Morita T,Takeuchi T

更新日期：1988-06-01 00:00:00

abstract：:The ETS family of transcription factors is defined by a conserved DNA-binding ETS domain that forms a winged helix-turn-helix structural motif. This family of transcription factors is involved in a diverse array of biological functions including cellular growth and differentiation, as well as organ development. Among ...

journal_title：Cell structure and function

authors： Sato Y

更新日期：2001-02-01 00:00:00

abstract：:The localization of mRNA in neuronal dendrites plays a role in both locally and temporally regulated protein synthesis, which is required for certain forms of synaptic plasticity. RNA granules constitute a dendritic mRNA transport machinery in neurons, which move along microtubules. RNA granules contain densely packed...

journal_title：Cell structure and function

authors： Miki T,Takano K,Yoneda Y

更新日期：2005-01-01 00:00:00

abstract：:In the present study, we compared the abilities of ricin and diphtheria toxin to induce apoptosis in Vero cells. The cytolysis and DNA fragmentation by ricin paralleled its protein synthesis inhibitory activity. However, unlike ricin, diphtheria toxin could induce neither cytolysis nor DNA fragmentation in Vero cells ...

journal_title：Cell structure and function

authors： Kusano I,Kageyama A,Tamura T,Oda T,Muramatsu T

更新日期：2001-10-01 00:00:00

abstract：:Multiple type I and II hair keratins are expressed in hair-forming cells but the role of each protein in hair fiber formation remains obscure. In this study, recombinant proteins of human type I hair keratins (K35, K36 and K38) and type II hair keratins (K81 and K85) were prepared using bacterial expression systems. T...

journal_title：Cell structure and function

authors： Honda Y,Koike K,Kubo Y,Masuko S,Arakawa Y,Ando S

更新日期：2014-01-01 00:00:00

abstract：:Here we report a new method of isolating epidermal desmosomes from Xenopus laevis, and a major constituent of desmosomes designated as Xenopus desmogleins (XDsg). Isolation of desmosomes from Xenopus laevis epidermis was carried out by a two step-incubation with different concentrations of NP-40. After discontinuous s...

journal_title：Cell structure and function

authors： Ohga R,Shida M,Shida H

更新日期：2004-02-01 00:00:00

abstract：:The effects of heavy ion microbeams on muscle fibers isolated from mouse skeletal muscles were examined by electron microscopy. The plasma membranes of heavy ion beam-irradiated areas of muscle fibers showed irregular protrusions and invaginations. In the cytoplasm, an irregular distribution of microfilaments was foun...

journal_title：Cell structure and function

authors： Hino M,Wada S,Tajika Y,Morimura Y,Hamada N,Funayama T,Sakashita T,Kakizaki T,Kobayashi Y,Yorifuji H

更新日期：2007-01-01 00:00:00

abstract：:Two methods of qualitative analysis of sequence distribution in DNA and protein are presented. The first method is based on the finding that the frequency of occurrence of each nucleotide in a defined sequence with functional significance more or less deviates from uniform distribution. The deviation found in this def...

journal_title：Cell structure and function

authors： Kiho Y

更新日期：1988-10-01 00:00:00

abstract：:Recent studies have demonstrated that vesicle transport to cleavage furrow is indispensable for cytokinesis. Some animal and plant cells form distinct structures during cell division known as central spindle and phragmoplast, respectively. Several essential factors involved in the vesicle transport have been isolated ...

journal_title：Cell structure and function

authors： Edamatsu M

更新日期：2001-12-01 00:00:00

abstract：:alpha-1,3-Fucosyltransferase (Fuc T IV) cDNA was placed under the control of beta-actin, cytomegalovirus enhancer/promoter and transfected into embryonic stem cells. The transfected cell clones with integrated cDNA (positive clones) differentiated more efficiently into myocardial cell clones without integrated cDNA (n...

journal_title：Cell structure and function

authors： Sudou A,Muramatsu H,Kaname T,Kadomatsu K,Muramatsu T

更新日期：1997-04-01 00:00:00

abstract：:Significant progress has been made toward our understanding of the basic principle of nucleocytoplasmic transport, and the structure of transport factors, as well as the diversity of nucleocytoplasmic transport pathways. This review outlines the current knowledge of transport, and discusses the problems that remain as...

journal_title：Cell structure and function

authors： Imamoto N

更新日期：2000-08-01 00:00:00

abstract：:We investigated the assembly of the first sarcomeres in chicken embryos by confocal microscopy of immunofluorescently stained whole mount rudiments of early chicken hearts isolated around the onset of beating. In embryos with merely 9 somites, myomesin was found to be present in a cross striated pattern, indicating th...

journal_title：Cell structure and function

authors： Auerbach D,Rothen-Ruthishauser B,Bantle S,Leu M,Ehler E,Helfman D,Perriard JC

更新日期：1997-02-01 00:00:00

abstract：:We have found that 1,9-Dideoxyforskolin (DDF) strongly inhibited the cell death induced by ricin, modeccin, Pseudomonas toxin, and diphtheria toxin in MDCK cells, suggesting that these protein toxins have a DDF-sensitive common pathway leading to cell death. However, no significant effect of forskolin on these toxins ...

journal_title：Cell structure and function

authors： Oda T,Komatsu N,Muramatsu T

更新日期：1997-10-01 00:00:00