Abstract:
:To study the effects of surface materials of cells on the behavior of other neighboring cells in a crowded culture, confluent sheets of rat 3Y1 fibroblasts were fixed and then 3Y1 cells were seeded on to them. Among confluent sheets unfixed, fixed with formalin and fixed with ethanol and an empty plastic dish surface, the substrate activity to permit cell adhesion was compared. After confluent 3Y1 cells (mainly composed of cells with a G1-DNA content) were reseeded with fresh medium on to these substrates, the capacity to initiate DNA synthesis per attached cell was also compared. The substrate activity of the ethanol-fixed cell sheet to permit cell adhesion was as high as that of the empty dish surface, whereas that of the unfixed cell sheet and that of the formalin-fixed cell sheet were low. When the ethanol-fixed cell sheet and the empty dish surface were coated with the ethanol extract of the unfixed cell sheet, the substrate activity diminished, indicating that during the fixation process with ethanol an adhesion-inhibitory factor (s) was removed. The capacity to initiate DNA synthesis of each cell that had completed adhesion and spreading on the cell sheets unfixed, fixed with formalin, and fixed with ethanol was lower compared to the cell that had adhered to the empty dish surface. We conclude that factors over the 3Y1 cell surface inhibit the overlapping cell adhesion and the proliferation of cells contacting each other, resulting in the ordered cell configuration in the confluent culture.
journal_name
Cell Struct Functjournal_title
Cell structure and functionauthors
Okuda A,Sasaki M,Kimura Gdoi
10.1247/csf.15.257subject
Has Abstractpub_date
1990-10-01 00:00:00pages
257-62issue
5eissn
0386-7196issn
1347-3700journal_volume
15pub_type
杂志文章abstract::Under unfavorable conditions for growth, haploid myxoamoebae of Physarum polycephalum retracted their pseudopodia and changed their cell shape into disk-like form, after which they constructed the cell walls to form microcysts. These morphological changes of haploid cells were associated with changes in intracellular ...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.17.301
更新日期:1992-10-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
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更新日期:2002-12-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
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journal_title:Cell structure and function
pub_type: 杂志文章
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更新日期:1989-04-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Cell structure and function
pub_type: 杂志文章
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journal_title:Cell structure and function
pub_type: 杂志文章
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更新日期:1994-04-01 00:00:00
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更新日期:2003-02-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.12.339
更新日期:1987-08-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.06025
更新日期:2006-01-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
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journal_title:Cell structure and function
pub_type: 杂志文章,评审
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更新日期:2001-12-01 00:00:00
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journal_title:Cell structure and function
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pub_type: 杂志文章,评审
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更新日期:2000-08-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.23.119
更新日期:1998-06-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.12.251
更新日期:1987-06-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
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更新日期:1994-12-01 00:00:00
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journal_title:Cell structure and function
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journal_title:Cell structure and function
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pub_type: 杂志文章
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更新日期:1991-10-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
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更新日期:1989-12-01 00:00:00
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更新日期:2008-01-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
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更新日期:1995-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-10-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
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更新日期:1994-02-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2005-01-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.16.125
更新日期:1991-04-01 00:00:00
abstract::Contact reaction is the morphological expression of allogeneic recognition by hemocytes in the ascidian, Halocynthia roretzi; namely they undergo an exocytotic burst upon contact with non-self (allogeneic) hemocytes in vitro. We have found that contact reaction is accompanied by a burst of cyanide-insensitive oxygen c...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.20.81
更新日期:1995-02-01 00:00:00