Heavy ion microbeam irradiation induces ultrastructural changes in isolated single fibers of skeletal muscle.

Abstract:

:The effects of heavy ion microbeams on muscle fibers isolated from mouse skeletal muscles were examined by electron microscopy. The plasma membranes of heavy ion beam-irradiated areas of muscle fibers showed irregular protrusions and invaginations. In the cytoplasm, an irregular distribution of microfilaments was found near the plasma membrane. Sarcoplasmic reticula in the irradiated regions showed a distended appearance with flocculent material within the lumen. These changes were seen as early as 2 min after irradiation, and persisted until as late as 22 min after irradiation. Many autophagic vacuoles could be seen at 7 min after irradiation. At 22 min, the vacuoles became more prominent and showed more variety. These observations suggest that heavy ion beam irradiation causes disruption of the cellular architecture and the autophagy is involved in removal of this disruption.

journal_name

Cell Struct Funct

authors

Hino M,Wada S,Tajika Y,Morimura Y,Hamada N,Funayama T,Sakashita T,Kakizaki T,Kobayashi Y,Yorifuji H

doi

10.1247/csf.06038

subject

Has Abstract

pub_date

2007-01-01 00:00:00

pages

51-6

issue

1

eissn

0386-7196

issn

1347-3700

pii

JST.JSTAGE/csf/06038

journal_volume

32

pub_type

杂志文章
  • Mitogen-mediated protein phosphorylation in Werner's syndrome fibroblasts.

    abstract::DNA synthesis of WF-1 fibroblasts derived from a patient with Werner's syndrome was stimulated by fetal calf serum and adult human serum but not by various mitogens including epidermal growth factor, platelet-derived growth factor (PDGF), fibroblast growth factor, insulin and 12-O-tetradecanoylphorbol-13-acetate (TPA)...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.13.471

    authors: Hirai M,Amagai M,Tajima S,Nishikawa T,Shimizu N

    更新日期:1988-12-01 00:00:00

  • Myelin formation in dissociated cell cultures of rat embryonic cerebral hemispheres.

    abstract::Developing cultures of dissociated cerebral hemispheres obtained from 18-day-old embryonic rats synthesized, or activated, a myelin-related enzyme, 2',3'-cyclic nucleotide 3'-phosphohydrolase (CNPase). This increase in activity coincided with the onset of myelination. The presence of CNPase in oligodendrocytes and mye...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.10.271

    authors: Hirano S,Iwasaki-Mutou N,Asou H,Ogawa Y

    更新日期:1985-09-01 00:00:00

  • Microtubule reorganization and lysosome redistribution by a viral v-src oncogene, in mouse Balb/3T3 cells expressing human EGF receptor.

    abstract::The epidermal growth factor (EGF)-induced endocytosis of its receptor is an obligatory pathway for the cellular regulation of the EGF-specific receptor (EGF-R). BNER4 is a mouse Balb/3T3 cell line transfected with human EGF-R complementary DNA (cDNA). B4/src-13 and B4/src-24 are BNER4 cells transfected with a viral on...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.19.397

    authors: Nakayama Y,Hisano T,Okimoto T,Tanaka Y,Ishikawa T,Himeno M,Ono M,Kuwano M

    更新日期:1994-12-01 00:00:00

  • Immunocytochemical evidence for centrosomal phosphoproteins in mitotic sea urchin eggs.

    abstract::The change in distribution of centrosomal phosphoproteins was examined in sea urchin eggs from fertilization to the first cleavage by immunofluorescence staining with the anti-phosphoprotein antibodies, MPM-1 and MPM-2. The antibodies reacted with female pronuclei in unfertilized eggs as well as centriolar complexes l...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.15.13

    authors: Kuriyama R,Rao PN,Borisy GG

    更新日期:1990-02-01 00:00:00

  • Nuclear translocation of the catalytic component of DNA-dependent protein kinase upon growth stimulation in normal human T lymphocytes.

    abstract::DNA-dependent protein kinase (DNA-PK), consisting of the 470-kDa catalytic component (DNA-PKcs) and the DNA-binding regulatory component Ku protein (p70/p80), catalyzes phosphorylation of a variety of DNA replication/transcription/repair factors in the presence of double-stranded DNA. In the resting states of human pe...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.22.585

    authors: Nagasawa M,Watanabe F,Suwa A,Yamamoto K,Tsukada K,Teraoka H

    更新日期:1997-12-01 00:00:00

  • Inhibitory effect of dideoxyforskolin on cell death induced by ricin, modeccin, diphtheria toxin, and Pseudomonas toxin in MDCK cells.

    abstract::We have found that 1,9-Dideoxyforskolin (DDF) strongly inhibited the cell death induced by ricin, modeccin, Pseudomonas toxin, and diphtheria toxin in MDCK cells, suggesting that these protein toxins have a DDF-sensitive common pathway leading to cell death. However, no significant effect of forskolin on these toxins ...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.22.545

    authors: Oda T,Komatsu N,Muramatsu T

    更新日期:1997-10-01 00:00:00

  • Monoclonal antibody with specificity to a conserved epitope in the C-terminal domain of histone H1 variants.

    abstract::A monoclonal type M-immunoglobulin (IgM) was generated in mice against a nuclease-urea extract of HeLa metaphase chromosomes. This antibody stains metaphase chromosomes from a variety of mammalian cultured cell types by indirect immunofluorescence. Antibody 12C7 reacts by western transfer technique with histone H1 in ...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.16.323

    authors: Ortiz M,Bejarano LA,Rendón MC,Martin G,Brinkley BR,Valdivia MM

    更新日期:1991-08-01 00:00:00

  • Sperm morphology and IMP distribution in membranes of spermatozoa of cyprinid fishes II.

    abstract::The morphology and intramembranous particle (IMP) distribution of spermatozoa were investigated in four species of cyprinid fishes, Gnathopogon elongatus elongatus, Pseudogobio esocinus, Acheilognathus lanceolatus and Puntius tetrazona. In particular, observation of IMP distribution was conducted as an aspect of compa...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.20.293

    authors: Ohta T,Matsuda M

    更新日期:1995-08-01 00:00:00

  • An altered nuclear migration into the daughter bud is induced by the cyclin A1-mediated Cdc28 kinase through an aberrant spindle movement in Saccharomyces cerevisiae.

    abstract::A strain of Saccharomyces cerevisiae that contains an integrated copy of a Xenopus cyclin A1 gene under the control of the GAL1 promoter has been constructed. On inducing expression of cyclin A1, the nuclear migration that occurs prior to division becomes aberrant. Instead of migrating to the neck between the mother c...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.22.465

    authors: Sikder H,Funakoshi M,Nishimoto T,Kobayashi H

    更新日期:1997-08-01 00:00:00

  • Pericentric H3K9me3 Formation by HP1 Interaction-defective Histone Methyltransferase Suv39h1.

    abstract::Pericentric regions form epigenetically organized, silent heterochromatin structures that accumulate histone H3 lysine 9 tri-methylation (H3K9me3) and heterochromatin protein 1 (HP1), a methylated H3K9-binding protein. At pericentric regions, Suv39h is the major enzyme that generates H3K9me3. Suv39h also interacts dir...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.16013

    authors: Muramatsu D,Kimura H,Kotoshiba K,Tachibana M,Shinkai Y

    更新日期:2016-12-03 00:00:00

  • Establishment and characterization of tracheal epithelial cell lines, TM01 and TM02-3, from transgenic mice bearing temperature-sensitive simian virus 40 large T-antigen gene.

    abstract::Murine tracheal epithelial cell lines, TM01 and TM02-3, were established from a primary culture of tracheal cells of adult transgenic mice bearing a temperature-sensitive simian virus (SV40) large T-antigen gene. Both TM01 and TM02-3 cells, which grew until confluent monolayers were formed, maintained tight contact wi...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.23.119

    authors: Sugiyama N,Tabuchi Y,Numata F,Uchida Y,Horiuchi T,Ishibashi K,Ono S,Obinata M,Furusawa M

    更新日期:1998-06-01 00:00:00

  • Immunocytochemical analysis of peptide hormone processing: importance of the positively charged N-terminal domain of signal peptide in correct ER targeting in yeast cells.

    abstract::We used a morphological approach to determine the topogenic role of the signal peptide in mediating the ER translocation of yeast prepro-alpha-factor. In prepro-alpha-factor-somatostatin hybrids, changes in the N-terminal amino acid sequence from wild-type NH2-Met-Arg-Phe (MRF) to NH2-Met-Phe-Lys (MFK) caused a subtle...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.22.365

    authors: Cheong KH,Park SD,Kim J,Hong SH

    更新日期:1997-06-01 00:00:00

  • Overexpression of PRA2, a Rab/Ypt-family small GTPase from Pea Pisum sativum, aggravates the growth defect of yeast ypt mutants.

    abstract::A large number of Rab/Ypt-family small GTPases have been identified from higher plants. While some of them can complement yeast ypt mutants, the expression of Arabidopsis Ara4 protein aggravated the growth defect of a subset of ypt mutants, probably because of the titration of common regulator(s) of yeast Ypt proteins...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.25.11

    authors: Matsuda N,Ueda T,Sasaki Y,Nakano A

    更新日期:2000-02-01 00:00:00

  • Le(X) structure enhances myocardial differentiation from embryonic stem cells.

    abstract::alpha-1,3-Fucosyltransferase (Fuc T IV) cDNA was placed under the control of beta-actin, cytomegalovirus enhancer/promoter and transfected into embryonic stem cells. The transfected cell clones with integrated cDNA (positive clones) differentiated more efficiently into myocardial cell clones without integrated cDNA (n...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.22.247

    authors: Sudou A,Muramatsu H,Kaname T,Kadomatsu K,Muramatsu T

    更新日期:1997-04-01 00:00:00

  • In vitro assembly properties of human type I and II hair keratins.

    abstract::Multiple type I and II hair keratins are expressed in hair-forming cells but the role of each protein in hair fiber formation remains obscure. In this study, recombinant proteins of human type I hair keratins (K35, K36 and K38) and type II hair keratins (K81 and K85) were prepared using bacterial expression systems. T...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.13021

    authors: Honda Y,Koike K,Kubo Y,Masuko S,Arakawa Y,Ando S

    更新日期:2014-01-01 00:00:00

  • The early secretory pathway contributes to autophagy in yeast.

    abstract::Autophagy is a starvation response in eukaryotes by which the cell delivers cytoplasmic components to the vacuole for degradation, and is mediated by a double membrane structure called the autophagosome. We have previously proposed that the specific combination of COPII like components, including Sec24p, is required f...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.28.49

    authors: Hamasaki M,Noda T,Ohsumi Y

    更新日期:2003-02-01 00:00:00

  • Chondroitin sulfate immobilized onto culture substrates modulates DNA synthesis, tyrosine aminotransferase induction, and intercellular communication in primary rat hepatocytes.

    abstract::Newly prepared phosphatidylethanolamine (PE) conjugates of glycosaminoglycans (GAGs) enabled us to immobilize GAGs to solid phase through hydrophobic interaction. Using these compounds, called GAG-PEs, we studied the effects of GAGs immobilized to culture plates coated with various extracellular matrix (ECM) proteins ...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.20.199

    authors: Kato S,Sugiura N,Kimata K,Kujiraoka T,Toyoda J,Akamatsu N

    更新日期:1995-06-01 00:00:00

  • A role of cofilin/destrin in reorganization of actin cytoskeleton in response to stresses and cell stimuli.

    abstract::1. Cofilin is an essential actin-regulating protein widely distributed in all eucaryotes. The structure and function of cofilin are conserved during evolution. 2. Cofilin depolymerizes F-actin in vitro at alkaline pH and severs F-actin in vitro at pH lower than 7.3. Overexpression of cofilin in viable cells induced bu...

    journal_title:Cell structure and function

    pub_type: 杂志文章,评审

    doi:10.1247/csf.21.421

    authors: Yahara I,Aizawa H,Moriyama K,Iida K,Yonezawa N,Nishida E,Hatanaka H,Inagaki F

    更新日期:1996-10-01 00:00:00

  • Modulation of microtubule dynamics by drugs: a paradigm for the actions of cellular regulators.

    abstract::Microtubules are intrinsically dynamic polymers. Two kinds of dynamic behaviors, dynamic instability and treadmilling, are important for microtubule function in cells. Both dynamic behaviors appear to be tightly regulated, but the cellular molecules and the mechanisms responsible for the regulation remain largely unex...

    journal_title:Cell structure and function

    pub_type: 杂志文章,评审

    doi:10.1247/csf.24.329

    authors: Wilson L,Panda D,Jordan MA

    更新日期:1999-10-01 00:00:00

  • Caffeine alone causes DNA damage in Chinese hamster ovary cells.

    abstract::Caffeine has been shown to enhance the lethal effect of DNA-damaging agents in mammalian cells, and the potentiation by caffeine of this effect is generally interpreted as the result of inhibition by caffeine of the repair of damaged DNA. However, the mechanism by which caffeine enhances the lethal effect of DNA-damag...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.10.405

    authors: Ishida R,Kozaki M,Takahashi T

    更新日期:1985-12-01 00:00:00

  • The molecular mechanism of targeted vesicle transport in cytokinesis.

    abstract::Recent studies have demonstrated that vesicle transport to cleavage furrow is indispensable for cytokinesis. Some animal and plant cells form distinct structures during cell division known as central spindle and phragmoplast, respectively. Several essential factors involved in the vesicle transport have been isolated ...

    journal_title:Cell structure and function

    pub_type: 杂志文章,评审

    doi:10.1247/csf.26.567

    authors: Edamatsu M

    更新日期:2001-12-01 00:00:00

  • The WD40 Domain of HIRA Is Essential for RI-nucleosome Assembly in Xenopus Egg Extracts.

    abstract::Histone chaperones are a group of histone-binding proteins that facilitate the assembly of nucleosomes, the fundamental structural units of chromatin in eukaryotes. In nucleosome assembly, deposition of a histone H3-H4 tetramer onto DNA is the first and critical step, which is mediated by the histone chaperones HIRA a...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.17001

    authors: Zhu R,Iwabuchi M,Ohsumi K

    更新日期:2017-01-01 00:00:00

  • Dispersion of Endoplasmic Reticulum-associated Compartments by 4-phenyl Butyric Acid in Yeast Cells.

    abstract::In yeast Saccharomyces cerevisiae cells, some aberrant multimembrane-spanning proteins are not transported to the cell surface but form and are accumulated in endoplasmic reticulum (ER)-derived subcompartments, known as the ER-associated compartments (ERACs), which are observed as puncta under fluorescence microscopy....

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.19023

    authors: Mai TC,Ishiwata-Kimata Y,Le QG,Kido H,Kimata Y

    更新日期:2019-11-23 00:00:00

  • Solubilization of aster-forming proteins from yeast: possible constituents of spindle pole body and reconstitution of asters in vitro.

    abstract::Spindle pole bodies (SPBs) attached to nuclei were isolated from Saccharomyces cerevisiae; they nucleated microtubules to form asters in vitro. This aster-forming activity was stable in 30% dimethylsulfoxide or 10% 2-propanol and could be solubilized with KCl. The KCl extract contained many protein components, which a...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.15.221

    authors: Yamamoto A,Nagai K,Yamasaki M,Matsuhashi M

    更新日期:1990-08-01 00:00:00

  • Involvement of prostaglandin-producing pathway in the cytotoxic action of tumor necrosis factor.

    abstract::To elucidate the cytotoxic mechanism of tumor necrosis factor (TNF), we isolated TNF-resistant sublines of L929 cells. As compared with L929 cells, TNF-resistant cells retained similar number and affinity of TNF-binding sites, and showed a similar growth rate. TNF stimulated arachidonate release from L929 cells, while...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.16.333

    authors: Hayakawa M,Oku N,Takagi T,Hori T,Shibamoto S,Yamanaka Y,Takeuchi K,Tsujimoto M,Ito F

    更新日期:1991-08-01 00:00:00

  • Inhibition of nuclear envelope reconstitution in Xenopus interphase egg extract by hemin.

    abstract::Addition of hemin to the nuclear reconstitution system of Xenopus interphase egg extract using sperm head chromatin resulted in abnormal pseudonuclei exhibiting flattened membrane patches randomly distributed both on the surface and inside the nuclei. The structures that resembled nuclear pores were observed on these ...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.23.291

    authors: Nishiyama R,Ikami M,Yamao F,Tsuchida S,Yagura T

    更新日期:1998-10-01 00:00:00

  • High voltage electric pulses efficiently induce fusion of cells in monolayer culture.

    abstract::High voltage electric pulses, 1000 V/cm, were found to induce cell fusion efficiently when delivered to cells growing in a monolayer culture. The maximum yield of fused cells was about 30% of cells remaining after treatment. Colonies of interspecies hybrid cells between mouse and others also appeared with a frequency ...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.14.173

    authors: Ishizaki K,Chang HR,Eguchi T,Ikenaga M

    更新日期:1989-04-01 00:00:00

  • Ca2+ translocation across liposomal membranes enhanced by unsaturated long-chain fatty acids.

    abstract::The putative ionophoretic action of phosphatidic acid or arachidonic acid metabolites for Ca2+ has offered an attractive explanation for stimulation-coupled mobilization of cytoplasmic Ca2+. We have examined the effects of Ca2+ ionophore and long-chain unsaturated fatty acids on the translocation of Ca2+ across the li...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.10.177

    authors: Utsumi K,Nobori K,Miyahara M

    更新日期:1985-06-01 00:00:00

  • Atg101: Not Just an Accessory Subunit in the Autophagy-initiation Complex.

    abstract::The Saccharomyces cerevisiae autophagy-initiation complex, Atg1 kinase complex, consists of Atg1, Atg13, Atg17, Atg29, and Atg31, while the corresponding complex in most other eukaryotes, including mammals, is composed of ULK1 (or ULK2), Atg13, FIP200 (also known as RB1CC1), and Atg101. ULKs are homologs of Atg1, and ...

    journal_title:Cell structure and function

    pub_type: 杂志文章,评审

    doi:10.1247/csf.15013

    authors: Noda NN,Mizushima N

    更新日期:2016-01-01 00:00:00

  • Binding of recombinant human cytokeratin 19 to laminin: a possible role in interaction between intermediate filament derived from epithelial cells and extracellular matrixes.

    abstract::Cytokeratin 8 (CK8) and cytokeratin 19 (CK19) is a specific cytoskeletal component of simple epithelia, including bronchial epithelial cells. We hypothesized that CK8 or CK19 released from epithelial cells may bind to and cause damage to extracellular matrixes through binding of anti-CK8 or anti-CK19 autoantibodies. I...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.25.171

    authors: Dobashi N,Fujita J,Murota M,Ohtsuki Y,Bandoh S,Ueda Y,Dohmoto K,Hojo S,Nishioka M,Ishida T,Takahara J

    更新日期:2000-06-01 00:00:00