Abstract:
:The precise control of spindle microtubule (MT) dynamics is essential for chromosome capture and alignment. Kif18A/kinesin-8, an essential regulator of kinetochore MT dynamics, accumulates at its plus-ends in metaphase but not prometaphase cells. The underlying mechanism of time-dependent and kinetochore MT-specific plus-end accumulation of Kif18A is unknown. Here, we examined the factors required for the MT plus-end accumulation of Kif18A. In Eg5 inhibitor-treated cells, Kif18A localized along the MTs in the monopolar spindle and rarely accumulated at their plus-ends, indicating that MT-kinetochore association was not sufficient to induce Kif18A accumulation. In contrast, taxol treatment triggered the rapid MT plus-end accumulation of Kif18A regardless of kinetochore association. Furthermore, Aurora B inhibitor-induced stabilization of the plus-ends of kinetochore MTs promoted the plus-end accumulation of Kif18A. In the absence of Kif18A, treatment with taxol but not Eg5 inhibitor causes highly elongated mitotic MTs, suggesting the importance of plus-end accumulation for the MT length-controlling activity of Kif18A. Taken together, we propose that there is a mutual regulation of kinetochore MT plus-end dynamics and Kif18A accumulation, which may contribute to the highly regulated and ordered changes in kinetochore MT dynamics during chromosome congression and oscillation.
journal_name
Cell Struct Functjournal_title
Cell structure and functionauthors
Masuda N,Shimodaira T,Shiu SJ,Tokai-Nishizumi N,Yamamoto T,Ohsugi Mdoi
10.1247/csf.11032subject
Has Abstractpub_date
2011-01-01 00:00:00pages
261-7issue
2eissn
0386-7196issn
1347-3700pii
JST.JSTAGE/csf/11032journal_volume
36pub_type
杂志文章abstract::Marvelous background rejection in total internal reflection fluorescence microscopy (TIR-FM) has made it possible to visualize single-fluorophores in living cells. Cell signaling proteins including peptide hormones, membrane receptors, small G proteins, cytoplasmic kinases as well as small signaling compounds have bee...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
doi:10.1247/csf.27.357
更新日期:2002-10-01 00:00:00
abstract::Human keratinocytes have been serially cultivated in low (0.015 mM) and high (1.8 mM) calcium containing medium. The calcium concentration of the growth medium significantly influenced the cell growth period in vitro. Cells grown in low calcium medium underwent 35-40 population doublings over 16-17 passages, while cel...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.12.539
更新日期:1987-12-01 00:00:00
abstract::In yeast Saccharomyces cerevisiae cells, some aberrant multimembrane-spanning proteins are not transported to the cell surface but form and are accumulated in endoplasmic reticulum (ER)-derived subcompartments, known as the ER-associated compartments (ERACs), which are observed as puncta under fluorescence microscopy....
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.19023
更新日期:2019-11-23 00:00:00
abstract::Embryonic stem (ES) cells are a pluripotent cell line derived from the inner cell mass of the mouse blastocyst. The mRNA levels of several growth-related genes were examined in ES cells by Northern blot analysis under several different growth conditions. In a coculture of ES cells with the mouse fibroblast cell line, ...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.16.375
更新日期:1991-10-01 00:00:00
abstract::An immunocytochemical method using a specific monoclonal antibody was employed to detect DNA polymerase alpha in Drosophila melanogaster embryos during the first 13 nuclear division cycles after fertilization. The anti-DNA polymerase alpha antibody stained the ooplasm of the unfertilized egg, indicating that DNA polym...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.17.105
更新日期:1992-04-01 00:00:00
abstract::The substance in sperm which induces the activation of eggs at fertilization has yet to be elucidated. Osawa et al. reported that the soluble extract of sperm (spex) causes inositol 1,4,5-trisphosphate (IP3)-induced Ca2+ release (IICR) in fertilized eggs of sea urchins when externally applied [Osawa et al., (1992). Zo...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.19.73
更新日期:1994-04-01 00:00:00
abstract::To elucidate the cytotoxic mechanism of tumor necrosis factor (TNF), we isolated TNF-resistant sublines of L929 cells. As compared with L929 cells, TNF-resistant cells retained similar number and affinity of TNF-binding sites, and showed a similar growth rate. TNF stimulated arachidonate release from L929 cells, while...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.16.333
更新日期:1991-08-01 00:00:00
abstract::CD9 and CD63 belong to a tetramembrane-spanning glycoprotein family called tetraspanin, and are involved in a wide variety of cellular processes, but the structure-function relationship of this family of proteins has yet to be clarified. CD9 associates with diphtheria toxin receptor (DTR), which is identical to the me...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.25.317
更新日期:2000-10-01 00:00:00
abstract::1. Cofilin is an essential actin-regulating protein widely distributed in all eucaryotes. The structure and function of cofilin are conserved during evolution. 2. Cofilin depolymerizes F-actin in vitro at alkaline pH and severs F-actin in vitro at pH lower than 7.3. Overexpression of cofilin in viable cells induced bu...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
doi:10.1247/csf.21.421
更新日期:1996-10-01 00:00:00
abstract::Pericentric regions form epigenetically organized, silent heterochromatin structures that accumulate histone H3 lysine 9 tri-methylation (H3K9me3) and heterochromatin protein 1 (HP1), a methylated H3K9-binding protein. At pericentric regions, Suv39h is the major enzyme that generates H3K9me3. Suv39h also interacts dir...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.16013
更新日期:2016-12-03 00:00:00
abstract::The endolymphatic sac (ES) is believed to play an important role in maintaining homeostasis in the inner ear by the absorption and endocytosis of endolymph. Megalin is a 600-kDa multiligand endocytic receptor expressed in certain types of absorptive epithelia including kidney proximal tubules. We analyzed the immunore...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.06013
更新日期:2006-01-01 00:00:00
abstract::Microtubules are intrinsically dynamic polymers. Two kinds of dynamic behaviors, dynamic instability and treadmilling, are important for microtubule function in cells. Both dynamic behaviors appear to be tightly regulated, but the cellular molecules and the mechanisms responsible for the regulation remain largely unex...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
doi:10.1247/csf.24.329
更新日期:1999-10-01 00:00:00
abstract::We have found that 1,9-Dideoxyforskolin (DDF) strongly inhibited the cell death induced by ricin, modeccin, Pseudomonas toxin, and diphtheria toxin in MDCK cells, suggesting that these protein toxins have a DDF-sensitive common pathway leading to cell death. However, no significant effect of forskolin on these toxins ...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.22.545
更新日期:1997-10-01 00:00:00
abstract::It has been suggested that the organization of microtubules during mitosis plays an important role in cytokinesis in animal cells. We studied the organization of microtubules during the first cleavage and its role in cytokinesis of Xenopus eggs. First, we examined the immunofluorescent localization of microtubules in ...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.27.163
更新日期:2002-08-01 00:00:00
abstract::Contact reaction is the morphological expression of allogeneic recognition by hemocytes in the ascidian, Halocynthia roretzi; namely they undergo an exocytotic burst upon contact with non-self (allogeneic) hemocytes in vitro. We have found that contact reaction is accompanied by a burst of cyanide-insensitive oxygen c...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.20.81
更新日期:1995-02-01 00:00:00
abstract::Caffeine has been shown to enhance the lethal effect of DNA-damaging agents in mammalian cells, and the potentiation by caffeine of this effect is generally interpreted as the result of inhibition by caffeine of the repair of damaged DNA. However, the mechanism by which caffeine enhances the lethal effect of DNA-damag...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.10.405
更新日期:1985-12-01 00:00:00
abstract::To construct recombinant retroviruses with only a single active promoter, we introduced point mutations into the TATA box region of the 3'-LTR, and successfully obtained high-titer virus with sufficient self-inactivating activity. However, the viral titer could not be determined by the number of G418 resistant colonie...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.20.177
更新日期:1995-04-01 00:00:00
abstract::Cells of Euglena gracilis Klebs var. bacillaris Cori mutant W3BUL grown in darkness on Hutner's pH 3.5 medium without agitation accumulate wax ester. These cells have undeveloped proplastid remnants characteristic of this mutant. If these cells are transferred to an inorganic medium and bubbled with 2-3% CO2 in air, t...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.12.453
更新日期:1987-10-01 00:00:00
abstract::Phosphorylation of regulatory light chain (RMLC) of myosin II at Ser19/Thr18 is likely to play important roles in controlling the morphological changes seen during cell division of cultured mammalian cells. Phosphorylation of RMLC regulates the activity of myosin II, an essntial motor for cytokinesis, and phosphorylat...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
doi:10.1247/csf.26.639
更新日期:2001-12-01 00:00:00
abstract::The efficacy of anticancer agents significantly depends on the differential susceptibility of undifferentiated cancer cells and differentiated normal cells to undergo apoptosis. We previously found that enhanced expression of RPS3a/nbl, which apparently encodes a ribosomal protein, seems to prime cells for apoptosis, ...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.25.103
更新日期:2000-04-01 00:00:00
abstract::tsJT60 is a nonlethal temperature-sensitive (ts) mutant of a Fischer rat cell line (3Y1) classified as a G0 mutant; i.e., the ts defect is not expressed within the cell growth cycle but is expressed only between the G0 and S phase. tsJT60 clones transformed with oncogenes such as adenovirus E1A, polyoma large T, polyo...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.15.385
更新日期:1990-12-01 00:00:00
abstract::Immunoblots of proteins extracted from the skin of a small viviparous fish (Xiphophorus) showed that a monoclonal antibody against human urokinase recognizes multiple molecular weight species of antigens. The immunoaffinity-purified antigens had serine-protease activity for the hydrolysis of a chromogenic substrate an...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.12.11
更新日期:1987-02-01 00:00:00
abstract::Histone modifications play critical roles in the epigenetic regulation of gene expression and in the maintenance of genome integrity. Acetylation and methylation of histone H3 are particularly important in gene activation and silencing. We generated and characterized a panel of mouse monoclonal antibodies that specifi...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.07035
更新日期:2008-01-01 00:00:00
abstract::Tobacco BY-2 cells were synchronized by an aphidicolin treatment, and their beta-tubulin isoforms and their mRNA were analyzed by Western, Northern and dot blottings. The relative ratio of the beta-tubulin isoforms changed with the progress of cell cycle stage. By Northern blot hybridization of poly(A)+RNAs with a clo...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.16.489
更新日期:1991-12-01 00:00:00
abstract::The cell cycle of HeLa S3 cells synchronized by hydroxyurea was investigated by flow cytometry. Metachromatic fluorochrome acridine orange was used to strain the DNA and RNA of the cells differentially. Periodic changes in the cellular DNA and RNA contents were observed through five cell cycles. The G1 and S phases of...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.8.303
更新日期:1983-09-01 00:00:00
abstract::Stimulation of polymorphonuclear leukocytes (PMN) by tetravalent concanavalin A (alpha-ConA) induces membrane depolarization preceding the onset of superoxide anion (O2-) production. Both divalent and monovalent ConA analogues were studied to evaluate the role of valence. Monovalent ConA (m-ConA) was inactive in stimu...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.11.199
更新日期:1986-06-01 00:00:00
abstract::Many studies on in vitro transformation of human cells indicate that the cells must be immortalized before they can be neoplastically transformed, indicating that immortalization is a critical step in multistep neoplastic transformation of human cells. We immortalized three human cell lines by repeated treatment with ...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.21.111
更新日期:1996-04-01 00:00:00
abstract::Checkpoint kinase 1 (Chk1) is a conserved protein kinase central to the cell-cycle checkpoint during DNA damage response (DDR). Until recently, ATR, a protein kinase activated in response to DNA damage or stalled replication, has been considered as the sole regulator of Chk1. Recent progress, however, has led to the i...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
doi:10.1247/csf.14017
更新日期:2015-01-01 00:00:00
abstract::We investigated the assembly of the first sarcomeres in chicken embryos by confocal microscopy of immunofluorescently stained whole mount rudiments of early chicken hearts isolated around the onset of beating. In embryos with merely 9 somites, myomesin was found to be present in a cross striated pattern, indicating th...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.22.139
更新日期:1997-02-01 00:00:00
abstract::Analysis of the amino acid sequence in protein (deviation analysis) suggests that the binding between trypsin and the enveloped virus is the first step of their interaction, which occurs in a specified configuration. It is possible that the distance between their active sites is important for the viral sensitivity to ...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.14.721
更新日期:1989-12-01 00:00:00