Abstract:
:Embryonic stem (ES) cells are a pluripotent cell line derived from the inner cell mass of the mouse blastocyst. The mRNA levels of several growth-related genes were examined in ES cells by Northern blot analysis under several different growth conditions. In a coculture of ES cells with the mouse fibroblast cell line, Balb/c3T3 A31, the expression level of transforming growth factor beta 1 (TGF-beta 1) mRNA was elevated for 1 to 3 days. In a coculture of ES cells with primary embryonic fibroblasts, only slight accumulation of TGF-beta 1 mRNA was observed for 6 to 24 h and soon attenuated. In situ hybridization analysis revealed that TGF-beta 1 transcripts accumulated first in the masses of ES cells and subsequently in Balb/c3T3 A31 cells adjacent to ES cells' masses. The ability of ES cells to induce TGF-beta 1 in Balb/c3T3 A31 cells was not mediated by soluble factors and was lost upon differentiation. ES cells on primary embryonic fibroblasts grew in undifferentiated form, but those on Balb/c3T3 A31 cells stopped growing and formed embryoid cystic bodies. We suggest that TGF-beta 1 mRNA induction in a coculture is triggered by an interaction between differentiating ES cells and Balb/c3T3 A31, and that this activity is limited to undifferentiated ES cells.
journal_name
Cell Struct Functjournal_title
Cell structure and functionauthors
Katsube K,Shimizu Ndoi
10.1247/csf.16.375subject
Has Abstractpub_date
1991-10-01 00:00:00pages
375-82issue
5eissn
0386-7196issn
1347-3700journal_volume
16pub_type
杂志文章abstract::1. Cofilin is an essential actin-regulating protein widely distributed in all eucaryotes. The structure and function of cofilin are conserved during evolution. 2. Cofilin depolymerizes F-actin in vitro at alkaline pH and severs F-actin in vitro at pH lower than 7.3. Overexpression of cofilin in viable cells induced bu...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
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journal_title:Cell structure and function
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journal_title:Cell structure and function
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journal_title:Cell structure and function
pub_type: 杂志文章
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journal_title:Cell structure and function
pub_type: 杂志文章
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更新日期:1992-04-01 00:00:00
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更新日期:1990-10-01 00:00:00
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journal_title:Cell structure and function
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更新日期:1987-06-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1997-04-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
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更新日期:2007-01-01 00:00:00
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journal_title:Cell structure and function
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journal_title:Cell structure and function
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更新日期:2015-01-01 00:00:00
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journal_title:Cell structure and function
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journal_title:Cell structure and function
pub_type: 杂志文章
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更新日期:1987-10-01 00:00:00
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journal_title:Cell structure and function
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journal_title:Cell structure and function
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journal_title:Cell structure and function
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journal_title:Cell structure and function
pub_type: 杂志文章
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