Abstract:
:Recent studies have revealed various biological functions for repetitive sequences, which make up about half of the human genome. One such sequence, major satellites, which are tandem repetitive sequences adjacent to the centromere, have been shown to be a kinetochore component that plays a role in the formation and function of the pericentric heterochromatin necessary for mitosis. However, it is unknown whether these regions also play a role in post-mitotic cells. Here, we show that, during neuronal differentiation, the heterochromatin domains that include major satellite regions become both enriched with the active histone modification lysine-4 trimethylation of histone H3, and more sensitive to nuclease, both of which suggest increased activation of this area. Further supporting this notion, we also found that transcription from major satellite regions is significantly increased during neuronal differentiation both in vitro and in vivo. These results together suggest that the structural and transcriptional state of major satellite regions changes dramatically during neuronal differentiation, implying that this region might play a role in differentiating neurons.
journal_name
Cell Struct Functjournal_title
Cell structure and functionauthors
Kishi Y,Kondo S,Gotoh Ydoi
10.1247/csf.12009subject
Has Abstractpub_date
2012-01-01 00:00:00pages
101-10issue
2eissn
0386-7196issn
1347-3700pii
DN/JST.JSTAGE/csf/12009journal_volume
37pub_type
杂志文章abstract::Previously, we serially transplanted tumors that produced colony-stimulating factor (CSF) into nude mice, who developed marked granulocytosis along with tumor growth; their leukocyte counts reaching approximately one million per cu mm. The numbers of CFU-GM, CFU-E, CFU-Meg, CFU-S and BFU-E were increased in nude mice ...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.12.273
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abstract::Murine tracheal epithelial cell lines, TM01 and TM02-3, were established from a primary culture of tracheal cells of adult transgenic mice bearing a temperature-sensitive simian virus (SV40) large T-antigen gene. Both TM01 and TM02-3 cells, which grew until confluent monolayers were formed, maintained tight contact wi...
journal_title:Cell structure and function
pub_type: 杂志文章
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journal_title:Cell structure and function
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journal_title:Cell structure and function
pub_type: 杂志文章,评审
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abstract::Spindle pole bodies (SPBs) attached to nuclei were isolated from Saccharomyces cerevisiae; they nucleated microtubules to form asters in vitro. This aster-forming activity was stable in 30% dimethylsulfoxide or 10% 2-propanol and could be solubilized with KCl. The KCl extract contained many protein components, which a...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.15.221
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journal_title:Cell structure and function
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journal_title:Cell structure and function
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.22.247
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journal_title:Cell structure and function
pub_type: 杂志文章
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journal_title:Cell structure and function
pub_type: 杂志文章
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journal_title:Cell structure and function
pub_type: 杂志文章
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更新日期:1989-04-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.10010
更新日期:2011-01-01 00:00:00
abstract::Thyroid follicles in vivo are embedded in extracellular matrix (ECM). The composing epithelial cells are in close contact with ECM at the basal side. To examine cell-to-ECM interactions, we studied adhesion, proliferation and differentiation of porcine follicle cells monolayer-cultured on type I and IV collagen, fibro...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.20.345
更新日期:1995-10-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.12.421
更新日期:1987-10-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章,评审
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更新日期:2001-12-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.23.291
更新日期:1998-10-01 00:00:00
abstract::A method has been developed to isolate pure preparations of nuclei in high yield from commercially available viable rice embryos (germ), employing extraction with buffer solution containing glycerol (without detergent) and polyamine, followed by centrifugation on a 30% Percoll cushion. The intactness of the isolated n...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.17.87
更新日期:1992-04-01 00:00:00
abstract::The proto-oncogene c-myc is a multifunctional gene that regulates cell division, cell growth, and apoptosis. Here we report a new function of c-myc: induction of autophagy. Autophagy is a bulk degradation system for intracellular proteins. Autophagy proceeds with characteristic morphologies, which begins with the form...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.28.195
更新日期:2003-06-01 00:00:00
abstract::We earlier discovered a novel 40-kDa protein (hsp40) induced by heat shock and other stresses in mammalian and avian cells. In this report, we purified the hsp40 in HeLa cells, using modified two-dimensional gel electrophoresis, and determined the amino terminal amino acid sequence of this protein. The hsp40 is homolo...
journal_title:Cell structure and function
pub_type: 杂志文章
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journal_title:Cell structure and function
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journal_title:Cell structure and function
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journal_title:Cell structure and function
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journal_title:Cell structure and function
pub_type: 杂志文章
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journal_title:Cell structure and function
pub_type: 杂志文章
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journal_title:Cell structure and function
pub_type: 杂志文章
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journal_title:Cell structure and function
pub_type: 杂志文章
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更新日期:2015-01-01 00:00:00
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journal_title:Cell structure and function
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journal_title:Cell structure and function
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更新日期:2018-02-16 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
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abstract::Epithelial tubules, consisting of the epithelial cell sheet with a central lumen, are the basic structure of many organs. Mechanical forces play an important role in epithelial tubulogenesis; however, little is known about the mechanisms controlling the mechanical forces during epithelial tubule morphogenesis. Solo (a...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.18010
更新日期:2018-06-02 00:00:00