Spatial localization of mono-and diphosphorylated myosin II regulatory light chain at the leading edge of motile HeLa cells.

abstract：:DNA-dependent protein kinase (DNA-PK), consisting of the 470-kDa catalytic component (DNA-PKcs) and the DNA-binding regulatory component Ku protein (p70/p80), catalyzes phosphorylation of a variety of DNA replication/transcription/repair factors in the presence of double-stranded DNA. In the resting states of human pe...

journal_title：Cell structure and function

authors： Nagasawa M,Watanabe F,Suwa A,Yamamoto K,Tsukada K,Teraoka H

更新日期：1997-12-01 00:00:00

abstract：:The ETS family of transcription factors is defined by a conserved DNA-binding ETS domain that forms a winged helix-turn-helix structural motif. This family of transcription factors is involved in a diverse array of biological functions including cellular growth and differentiation, as well as organ development. Among ...

journal_title：Cell structure and function

authors： Sato Y

更新日期：2001-02-01 00:00:00

abstract：:The morphology and intramembranous particle (IMP) distribution of spermatozoa were investigated in four species of cyprinid fishes, Gnathopogon elongatus elongatus, Pseudogobio esocinus, Acheilognathus lanceolatus and Puntius tetrazona. In particular, observation of IMP distribution was conducted as an aspect of compa...

journal_title：Cell structure and function

authors： Ohta T,Matsuda M

更新日期：1995-08-01 00:00:00

abstract：:To construct recombinant retroviruses with only a single active promoter, we introduced point mutations into the TATA box region of the 3'-LTR, and successfully obtained high-titer virus with sufficient self-inactivating activity. However, the viral titer could not be determined by the number of G418 resistant colonie...

journal_title：Cell structure and function

authors： Miyao Y,Shimizu K,Tamura M,Yamada M,Tamura K,Nakahira K,Kuriyama S,Hayakawa T,Ikenaka K

更新日期：1995-04-01 00:00:00

abstract：:In adult rat cerebellum, HPC-1/syntaxin 1 is detected at high density on the plasma membrane of the non-synaptic region of parallel fibers in addition to the synaptic terminal membranes and the synaptic vesicles (Koh, S., Yamamoto, A., Inoue, A., Inoue, Y., Akagawa, Y., Kawamura, Y., Kawamoto, Y., and Tashiro, Y. (199...

journal_title：Cell structure and function

authors： Miya F,Yamamoto A,Akagawa K,Kawamoto K,Tashiro Y

更新日期：1996-12-01 00:00:00

abstract：:Upon tryptic digestion of synaptosomes, ATPase activities decreased in the order of Cl(-)-ATPase greater than or equal to Na+,K+-ATPase greater than anion-insensitive Mg2+-ATPase. Upon synaptosome treatment with hypotonic solution, Cl(-)-ATPase or anion-insensitive Mg2+-ATPase was slightly inactivated, while Na+,K+-AT...

journal_title：Cell structure and function

authors： Kunugi-Uehara Y,Hashimoto Y,Ogura C,Inagaki C,Nishino T

更新日期：1988-04-01 00:00:00

abstract：:Here we describe the design and synthesis of a bifunctional two-photon fluorescence probe, N,N'-‍dimethyl-4,4'-(biphenyl-2,1-ethenediyl)dipyridinium hexafluorophosphate (BP6). HeLa, Hek293, and Paramecium caudatum cells were stained with BP6. BP6 accumulated on the mitochondria of all three cell types when the mitocho...

journal_title：Cell structure and function

authors： Moritomo H,Yamada K,Kojima Y,Suzuki Y,Tani S,Kinoshita H,Sasaki A,Mikuni S,Kinjo M,Kawamata J

更新日期：2014-01-01 00:00:00

abstract：:Recent studies have revealed various biological functions for repetitive sequences, which make up about half of the human genome. One such sequence, major satellites, which are tandem repetitive sequences adjacent to the centromere, have been shown to be a kinetochore component that plays a role in the formation and f...

journal_title：Cell structure and function

authors： Kishi Y,Kondo S,Gotoh Y

更新日期：2012-01-01 00:00:00

abstract：:Tritrichomonas foetus strongly agglutinates human erythrocytes, thus suggesting the occurrence of an adhesin associated with its surface. Adherence was observed immediately after mixing of the parasites with erythrocytes and the intensity increased for up to 30 min. Scanning electron microscopy examination of T. foetu...

journal_title：Cell structure and function

authors： da Silva NS,Dias Filho BP,De Souza W

更新日期：1996-08-01 00:00:00

abstract：:The fine structural localization of albumin in rat liver parenchymal cells was determined by an improved immunocytochemical method and serial sectioning. Albumin in the secretory apparatus of the parenchymal cells was present in segments of the rough endoplasmic reticulum, interrupted with negative segments, in transp...

journal_title：Cell structure and function

authors： Yokota S,Fahimi DH

更新日期：1987-06-01 00:00:00

abstract：:Mouse embryonic stem (mES) cells can be maintained in undifferentiated state in the presence of a cytokine, leukemia inhibitory factor (LIF). Many investigators found that STAT3 activation is important for the maintenance of pluripotency by LIF. However, the downstream pathways of STAT3 activation are still unknown. T...

journal_title：Cell structure and function

authors： Ko SY,Kang HY,Lee HS,Han SY,Hong SH

更新日期：2006-01-01 00:00:00

abstract：:A monoclonal type M-immunoglobulin (IgM) was generated in mice against a nuclease-urea extract of HeLa metaphase chromosomes. This antibody stains metaphase chromosomes from a variety of mammalian cultured cell types by indirect immunofluorescence. Antibody 12C7 reacts by western transfer technique with histone H1 in ...

journal_title：Cell structure and function

authors： Ortiz M,Bejarano LA,Rendón MC,Martin G,Brinkley BR,Valdivia MM

更新日期：1991-08-01 00:00:00

abstract：:The cell cycle of HeLa S3 cells synchronized by hydroxyurea was investigated by flow cytometry. Metachromatic fluorochrome acridine orange was used to strain the DNA and RNA of the cells differentially. Periodic changes in the cellular DNA and RNA contents were observed through five cell cycles. The G1 and S phases of...

journal_title：Cell structure and function

authors： Fujikawa-Yamamoto K

更新日期：1983-09-01 00:00:00

abstract：:Ran is a nuclear Ras-like GTPase that is required for various nuclear events including the bi-directional transport of proteins and ribonucleoproteins through the nuclear pore complex, spindle formation, and reassembly of the nuclear envelope. One of the key regulators of Ran is RanGAP1, a Ran specific GTPase activati...

journal_title：Cell structure and function

authors： Takeda E,Hieda M,Katahira J,Yoneda Y

更新日期：2005-01-01 00:00:00

abstract：:Thyroid follicles in vivo are embedded in extracellular matrix (ECM). The composing epithelial cells are in close contact with ECM at the basal side. To examine cell-to-ECM interactions, we studied adhesion, proliferation and differentiation of porcine follicle cells monolayer-cultured on type I and IV collagen, fibro...

journal_title：Cell structure and function

authors： Toda S,Matsumura S,Yonemitsu N,Fujitani N,Terayama K,Funatsumaru S,Sugihara H

更新日期：1995-10-01 00:00:00

abstract：:Murine tracheal epithelial cell lines, TM01 and TM02-3, were established from a primary culture of tracheal cells of adult transgenic mice bearing a temperature-sensitive simian virus (SV40) large T-antigen gene. Both TM01 and TM02-3 cells, which grew until confluent monolayers were formed, maintained tight contact wi...

journal_title：Cell structure and function

authors： Sugiyama N,Tabuchi Y,Numata F,Uchida Y,Horiuchi T,Ishibashi K,Ono S,Obinata M,Furusawa M

更新日期：1998-06-01 00:00:00

abstract：:It has been shown that in PC12 and its subclone PC12h treatment of the cells with nerve growth factor (NGF) induces a selective decrease in the incorporation of radioactive phosphate into a 100,000-dalton protein, designated in an earlier study as Nsp100, in the subsequent phosphorylation of soluble extracts from cell...

journal_title：Cell structure and function

authors： Hashimoto S,Hagino A

更新日期：1989-02-01 00:00:00

abstract：:Significant progress has been made toward our understanding of the basic principle of nucleocytoplasmic transport, and the structure of transport factors, as well as the diversity of nucleocytoplasmic transport pathways. This review outlines the current knowledge of transport, and discusses the problems that remain as...

journal_title：Cell structure and function

authors： Imamoto N

更新日期：2000-08-01 00:00:00

abstract：:Short-term and long-term (greater than 7 months) cultured astrocytes from 14-day-old rat brain were analyzed for ganglioside content. Analysis of the extracted gangliosides by HPTLC revealed that ganglioside GM1 was absent in 35 days and 235 days cultured astrocytes, and that the predominant ganglioside was GM3, showi...

journal_title：Cell structure and function

authors： Asou H,Hirano S,Uyemura K

更新日期：1989-10-01 00:00:00

abstract：:Marvelous background rejection in total internal reflection fluorescence microscopy (TIR-FM) has made it possible to visualize single-fluorophores in living cells. Cell signaling proteins including peptide hormones, membrane receptors, small G proteins, cytoplasmic kinases as well as small signaling compounds have bee...

journal_title：Cell structure and function

authors： Sako Y,Uyemura T

更新日期：2002-10-01 00:00:00

abstract：:The purpose of the present study is to analyze the effect of serum or chick embryo extract (CEE) on the neuronal differentiation of the mouse neural crest cells. When the crest cells were cultured in the medium containing serum at low concentration (5% calf serum), neurite outgrowth was observed. The active outgrowth ...

journal_title：Cell structure and function

authors： Ito K,Morita T,Takeuchi T

更新日期：1988-06-01 00:00:00

abstract：:Interleukin 2 (IL-2) initiated proliferation of the cells of T-enriched population stimulated with non-mitogenic dose of concanavalin A (Con A), whereas it could not induce proliferation of the cells treated with non-mitogenic lectin wheat germ agglutinin (WGA). Use of monoclonal antibody (MAb) directed against interl...

journal_title：Cell structure and function

authors： Komada H,Nakabayashi H,Hara M,Hara M,Takahashi T,Morita J,Yoshida T,Takanari H,Izutsu K

更新日期：1989-04-01 00:00:00

abstract：:Here, we would like to point out important milestones in the study of nuclear radial positioning and gene expression during differentiation processes. In addition, changes in the histone signature that significantly precede various differentiation pathways are reviewed. We address the regulatory functions of chromatin...

journal_title：Cell structure and function

authors： Harnicarová Horáková A,Bártová E,Kozubek S

更新日期：2010-01-01 00:00:00

abstract：:Recent studies have demonstrated that vesicle transport to cleavage furrow is indispensable for cytokinesis. Some animal and plant cells form distinct structures during cell division known as central spindle and phragmoplast, respectively. Several essential factors involved in the vesicle transport have been isolated ...

journal_title：Cell structure and function

authors： Edamatsu M

更新日期：2001-12-01 00:00:00

abstract：:Epidermal growth factor (EGF) was derivatized at the amino terminus with N-succinimidyl 3-(2-pyridyldithio)propionate and then cross-linked to the cysteinyl residues of alpha 2-macroglobulin (alpha 2M) via disulfide bonds. The EGF-alpha 2M conjugate delivered EGF into dense lysosomal fractions through binding to alpha...

journal_title：Cell structure and function

authors： Ito F,Ito S,Shimizu N

更新日期：1984-06-01 00:00:00

abstract：:tsJT60, a temperature-sensitive (ts) G0-mutant cell line from a Fischer rat, grows normally in the exponential growth phase at 34 degrees C and 39.5 degrees C, but when stimulated with fetal bovine serum (FBS), from the G0 phase they reenter the S phase at 34 degrees C but not at 39.5 degrees C. The ts-block was bypas...

journal_title：Cell structure and function

authors： Ninomiya-Tsuji J,Nakahara Y,Ito C,Akiyama T,Ishibashi S,Ide T

更新日期：1987-10-01 00:00:00

abstract：:Two methods of qualitative analysis of sequence distribution in DNA and protein are presented. The first method is based on the finding that the frequency of occurrence of each nucleotide in a defined sequence with functional significance more or less deviates from uniform distribution. The deviation found in this def...

journal_title：Cell structure and function

authors： Kiho Y

更新日期：1988-10-01 00:00:00

abstract：:Cultured hepatic stellate cells (HSCs) are known to change their morphology and function with respect to the production of extracellular matrices (ECMs) and matrix metalloproteinases (MMPs) in response to ECM components. We examined the regulatory role of the native form of type I collagen fibrils in pro-MMP-2 product...

journal_title：Cell structure and function

authors： Wang DR,Sato M,Li LN,Miura M,Kojima N,Senoo H

更新日期：2003-12-01 00:00:00

abstract：:The effects of heavy ion microbeams on muscle fibers isolated from mouse skeletal muscles were examined by electron microscopy. The plasma membranes of heavy ion beam-irradiated areas of muscle fibers showed irregular protrusions and invaginations. In the cytoplasm, an irregular distribution of microfilaments was foun...

journal_title：Cell structure and function

authors： Hino M,Wada S,Tajika Y,Morimura Y,Hamada N,Funayama T,Sakashita T,Kakizaki T,Kobayashi Y,Yorifuji H

更新日期：2007-01-01 00:00:00

abstract：:A granulocyte colony-stimulating factor (G-CSF) was highly purified from the serum-free culture medium of RSP -2 X P3 cells. The G-CSF had an apparent molecular weight of 33,000 as determined by high speed gel permeation chromatography, but its molecular weight was decreased to 15,000 by 0.1% sodium dodecyl sulfate. A...

journal_title：Cell structure and function

authors： Tsuneoka K,Shikita M

更新日期：1984-03-01 00:00:00