Abstract:
:DNA-dependent protein kinase (DNA-PK), consisting of the 470-kDa catalytic component (DNA-PKcs) and the DNA-binding regulatory component Ku protein (p70/p80), catalyzes phosphorylation of a variety of DNA replication/transcription/repair factors in the presence of double-stranded DNA. In the resting states of human peripheral blood mononuclear cells, DNA-PK activity and the protein level of DNA-PKcs were very low in the nuclear extracts, but they were high in the whole cell extracts. Depending upon proliferation of the T lymphocytes, DNA-PK activity and the protein level of DNA-PKcs in the nuclear extracts greatly increased. Immunocytochemical analysis suggested translocation of DNA-PKcs from the cytoplasm to the nucleus upon growth stimulation in the T lymphocytes.
journal_name
Cell Struct Functjournal_title
Cell structure and functionauthors
Nagasawa M,Watanabe F,Suwa A,Yamamoto K,Tsukada K,Teraoka Hdoi
10.1247/csf.22.585subject
Has Abstractpub_date
1997-12-01 00:00:00pages
585-94issue
6eissn
0386-7196issn
1347-3700journal_volume
22pub_type
杂志文章abstract::Embryonic stem (ES) cells are a pluripotent cell line derived from the inner cell mass of the mouse blastocyst. The mRNA levels of several growth-related genes were examined in ES cells by Northern blot analysis under several different growth conditions. In a coculture of ES cells with the mouse fibroblast cell line, ...
journal_title:Cell structure and function
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journal_title:Cell structure and function
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journal_title:Cell structure and function
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journal_title:Cell structure and function
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journal_title:Cell structure and function
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journal_title:Cell structure and function
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journal_title:Cell structure and function
pub_type: 杂志文章,评审
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更新日期:2001-12-01 00:00:00
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journal_title:Cell structure and function
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更新日期:2006-01-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.14.173
更新日期:1989-04-01 00:00:00
abstract::Addition of hemin to the nuclear reconstitution system of Xenopus interphase egg extract using sperm head chromatin resulted in abnormal pseudonuclei exhibiting flattened membrane patches randomly distributed both on the surface and inside the nuclei. The structures that resembled nuclear pores were observed on these ...
journal_title:Cell structure and function
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1984-06-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.24.419
更新日期:1999-10-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.19.73
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journal_title:Cell structure and function
pub_type: 杂志文章
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journal_title:Cell structure and function
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journal_title:Cell structure and function
pub_type: 杂志文章
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更新日期:1995-06-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章,评审
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.10.177
更新日期:1985-06-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
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journal_title:Cell structure and function
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journal_title:Cell structure and function
pub_type: 杂志文章
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journal_title:Cell structure and function
pub_type: 杂志文章,评审
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更新日期:2010-01-01 00:00:00