Establishment and characterization of tracheal epithelial cell lines, TM01 and TM02-3, from transgenic mice bearing temperature-sensitive simian virus 40 large T-antigen gene.

abstract：:The efficacy of anticancer agents significantly depends on the differential susceptibility of undifferentiated cancer cells and differentiated normal cells to undergo apoptosis. We previously found that enhanced expression of RPS3a/nbl, which apparently encodes a ribosomal protein, seems to prime cells for apoptosis, ...

journal_title：Cell structure and function

authors： Russell L,Naora H,Naora H

更新日期：2000-04-01 00:00:00

abstract：:The Golgi stress response is a homeostatic mechanism that controls the capacity of the Golgi apparatus in accordance with cellular demands. When the capacity of the Golgi apparatus becomes insufficient (Golgi stress), transcription levels of Golgi-related genes encoding glycosylation enzymes, a Golgi structural protei...

journal_title：Cell structure and function

authors： Taniguchi M,Sasaki-Osugi K,Oku M,Sawaguchi S,Tanakura S,Kawai Y,Wakabayashi S,Yoshida H

更新日期：2016-07-30 00:00:00

abstract：:The distribution of Ca2+ in dividing cells of the maize root tip was examined by potassium pyroantimonate precipitation and EGTA treatment methods. Ca2+ was found in most of the cell organelles, such as the matrix of the mitochondria, the thylakoid membrane of the proplastid and the Golgi vesicles, and on the plasma m...

journal_title：Cell structure and function

authors： Sakai-Wada A,Yagi S

更新日期：1993-12-01 00:00:00

abstract：:Vascular endothelial cells possess antithrombotic properties, which are determined by the balance between plasminogen activators (PAs) and PA inhibitors (PAls). A cell line, TKM-33, has been established and cloned from human umbilical vein endothelial cells, was previously reported to produce a large amount of urokina...

journal_title：Cell structure and function

authors： Ueshima S,Matsumoto H,Izaki S,Mitsui Y,Fukao H,Okada K,Matsuo O

更新日期：1999-04-01 00:00:00

abstract：:An immunocytochemical method using a specific monoclonal antibody was employed to detect DNA polymerase alpha in Drosophila melanogaster embryos during the first 13 nuclear division cycles after fertilization. The anti-DNA polymerase alpha antibody stained the ooplasm of the unfertilized egg, indicating that DNA polym...

journal_title：Cell structure and function

authors： Yamaguchi M,Kuroda K,Hirose F,Matsukage A

更新日期：1992-04-01 00:00:00

abstract：:A granulocyte colony-stimulating factor (G-CSF) was highly purified from the serum-free culture medium of RSP -2 X P3 cells. The G-CSF had an apparent molecular weight of 33,000 as determined by high speed gel permeation chromatography, but its molecular weight was decreased to 15,000 by 0.1% sodium dodecyl sulfate. A...

journal_title：Cell structure and function

authors： Tsuneoka K,Shikita M

更新日期：1984-03-01 00:00:00

abstract：:We constructed a fusion protein of the cell-binding domain of human fibronectin and human basic fibroblast growth factor, and prepared a polypeptide with both cell-adhesive activity and growth factor activity. A human gene fragment coding for basic fibroblast growth factor was amplified by the polymerase chain reactio...

journal_title：Cell structure and function

authors： Hashi H,Hatai M,Kimizuka F,Kato I,Yaoi Y

更新日期：1994-02-01 00:00:00

abstract：:The transcription factor ATF6 is constitutively synthesized as a type II transmembrane protein embedded in the endoplasmic reticulum (ER). When unfolded proteins accumulate in the ER, ATF6 senses such ER stress via an as yet undetermined mechanism and relocates to the Golgi apparatus where it is cleaved by sequential ...

journal_title：Cell structure and function

authors： Sato Y,Nadanaka S,Okada T,Okawa K,Mori K

更新日期：2011-01-01 00:00:00

abstract：:The involvement of actin filaments from the host cell on the process of invasion of trypomastigote forms of Trypanosma cruzi was analyzed in seven different cell lines. Prior incubation of all cell lines with cytochalasin D, under conditions which interfere with actin filaments, markedly inhibited parasite internaliza...

journal_title：Cell structure and function

authors： Rosestolato CT,Dutra Jda M,De Souza W,de Carvalho TM

更新日期：2002-04-01 00:00:00

abstract：:Histone chaperones are a group of histone-binding proteins that facilitate the assembly of nucleosomes, the fundamental structural units of chromatin in eukaryotes. In nucleosome assembly, deposition of a histone H3-H4 tetramer onto DNA is the first and critical step, which is mediated by the histone chaperones HIRA a...

journal_title：Cell structure and function

authors： Zhu R,Iwabuchi M,Ohsumi K

更新日期：2017-01-01 00:00:00

abstract：:Here we report a new method of isolating epidermal desmosomes from Xenopus laevis, and a major constituent of desmosomes designated as Xenopus desmogleins (XDsg). Isolation of desmosomes from Xenopus laevis epidermis was carried out by a two step-incubation with different concentrations of NP-40. After discontinuous s...

journal_title：Cell structure and function

authors： Ohga R,Shida M,Shida H

更新日期：2004-02-01 00:00:00

abstract：:Effects of kinase inhibitors on the preprophase band of microtubules in onion (Allium cepa L.) root tip cells were examined. Bundled microtubules in preprophase bands were dispersed on the cell cortex when onion seedlings were incubated with 2.5-5.0 mM 6-dimethylaminopurine. Fifteen min was enough for the bundled micr...

journal_title：Cell structure and function

authors： Nogami A,Mineyuki Y

更新日期：1999-10-01 00:00:00

abstract：:The ETS family of transcription factors is defined by a conserved DNA-binding ETS domain that forms a winged helix-turn-helix structural motif. This family of transcription factors is involved in a diverse array of biological functions including cellular growth and differentiation, as well as organ development. Among ...

journal_title：Cell structure and function

authors： Sato Y

更新日期：2001-02-01 00:00:00

abstract：:It has been shown that in PC12 and its subclone PC12h treatment of the cells with nerve growth factor (NGF) induces a selective decrease in the incorporation of radioactive phosphate into a 100,000-dalton protein, designated in an earlier study as Nsp100, in the subsequent phosphorylation of soluble extracts from cell...

journal_title：Cell structure and function

authors： Hashimoto S,Hagino A

更新日期：1989-02-01 00:00:00

abstract：:Newly prepared phosphatidylethanolamine (PE) conjugates of glycosaminoglycans (GAGs) enabled us to immobilize GAGs to solid phase through hydrophobic interaction. Using these compounds, called GAG-PEs, we studied the effects of GAGs immobilized to culture plates coated with various extracellular matrix (ECM) proteins ...

journal_title：Cell structure and function

authors： Kato S,Sugiura N,Kimata K,Kujiraoka T,Toyoda J,Akamatsu N

更新日期：1995-06-01 00:00:00

abstract：:NBT-II cells on collagen-coated substrates move rapidly and persistently, maintaining a semi-circular shape with a large lamellipodium, in a manner similar to fish keratocytes. The inhibitor of phospholipase D (PLD), n-butanol, completely blocked the migration and disturbed the characteristic localization of actin alo...

journal_title：Cell structure and function

authors： Nagasaki A,Inotsume K,Kanada M,Uyeda TQ

更新日期：2008-01-01 00:00:00

abstract：:Human keratinocytes have been serially cultivated in low (0.015 mM) and high (1.8 mM) calcium containing medium. The calcium concentration of the growth medium significantly influenced the cell growth period in vitro. Cells grown in low calcium medium underwent 35-40 population doublings over 16-17 passages, while cel...

journal_title：Cell structure and function

authors： Rockwell GA,Johnson G,Sibatani A

更新日期：1987-12-01 00:00:00

abstract：:When spores of Dictyostelium discoideum matured through normal development were stained with rhodamine-phalloidin, rod-shaped fluorescence was found across the nucleus and in the cytoplasm. Electron micrographs of the rod shape showed that it consisted of long and straight fibers 12 nm in diameter. In contrast, a conv...

journal_title：Cell structure and function

authors： Sameshima M,Chijiiwa Y,Kishi Y,Hashimoto Y

更新日期：1994-08-01 00:00:00

abstract：:Numerous types of cancer cells migrate into extracellular tissues. This phenomenon is termed invasion, and is associated with poor prognosis in cancer patients. In this study, we demonstrated that filamin B (FLNb), an actin-binding protein, is highly expressed in cancer cell lines that exhibit high invasiveness, with ...

journal_title：Cell structure and function

authors： Iguchi Y,Ishihara S,Uchida Y,Tajima K,Mizutani T,Kawabata K,Haga H

更新日期：2015-01-01 00:00:00

abstract：:Two methods of qualitative analysis of sequence distribution in DNA and protein are presented. The first method is based on the finding that the frequency of occurrence of each nucleotide in a defined sequence with functional significance more or less deviates from uniform distribution. The deviation found in this def...

journal_title：Cell structure and function

authors： Kiho Y

更新日期：1988-10-01 00:00:00

abstract：:We used a morphological approach to determine the topogenic role of the signal peptide in mediating the ER translocation of yeast prepro-alpha-factor. In prepro-alpha-factor-somatostatin hybrids, changes in the N-terminal amino acid sequence from wild-type NH2-Met-Arg-Phe (MRF) to NH2-Met-Phe-Lys (MFK) caused a subtle...

journal_title：Cell structure and function

authors： Cheong KH,Park SD,Kim J,Hong SH

更新日期：1997-06-01 00:00:00

abstract：:We have previously shown that mouse L.P3 cells secrete fibronectin and a novel protein, gelatin-non-binding and heparin-binding cell-adhesive protein (GNCP). Here, we screened and characterized cell-adhesive proteins in the conditioned media (CM's) of a series of cell lines growing in a protein- and lipid-free synthet...

journal_title：Cell structure and function

authors： Morita H,Yasumitsu H,Watanabe Y,Miyazaki K,Umeda M

更新日期：1993-02-01 00:00:00

abstract：:The Saccharomyces cerevisiae autophagy-initiation complex, Atg1 kinase complex, consists of Atg1, Atg13, Atg17, Atg29, and Atg31, while the corresponding complex in most other eukaryotes, including mammals, is composed of ULK1 (or ULK2), Atg13, FIP200 (also known as RB1CC1), and Atg101. ULKs are homologs of Atg1, and ...

journal_title：Cell structure and function

authors： Noda NN,Mizushima N

更新日期：2016-01-01 00:00:00

abstract：:We have found that 1,9-Dideoxyforskolin (DDF) strongly inhibited the cell death induced by ricin, modeccin, Pseudomonas toxin, and diphtheria toxin in MDCK cells, suggesting that these protein toxins have a DDF-sensitive common pathway leading to cell death. However, no significant effect of forskolin on these toxins ...

journal_title：Cell structure and function

authors： Oda T,Komatsu N,Muramatsu T

更新日期：1997-10-01 00:00:00

abstract：:To evaluate the role of VAMP8/endobrevin in constitutive exocytosis, we have examined the exocytotic pathways of VAMP8 and human growth hormone, both GFP-tagged, by total internal reflection fluorescence microscopy (TIRF-M). Human GH-GFP and VAMP8-GFP were similarly expressed in small round vesicles and elongated tubu...

journal_title：Cell structure and function

authors： Okayama M,Arakawa T,Tanimura A,Mizoguchi I,Tajima Y,Takuma T

更新日期：2009-01-01 00:00:00

abstract：:CD9 and CD63 belong to a tetramembrane-spanning glycoprotein family called tetraspanin, and are involved in a wide variety of cellular processes, but the structure-function relationship of this family of proteins has yet to be clarified. CD9 associates with diphtheria toxin receptor (DTR), which is identical to the me...

journal_title：Cell structure and function

authors： Ryu F,Takahashi T,Nakamura K,Takahashi Y,Kobayashi T,Shida S,Kameyama T,Mekada E

更新日期：2000-10-01 00:00:00

abstract：:The amount of free calcium in the cytoplasm is important in stimulation coupled with a number of cellular functions. The putative ionophoretic action of membrane lipid metabolites on Ca2+ offers convenient explanation of the stimulation-coupled mobilization of cytoplasmic Ca2+. To analyze the ionophoretic action of th...

journal_title：Cell structure and function

authors： Utsumi K,Nobori K,Terada S,Miyahara M,Utsumi T

更新日期：1985-12-01 00:00:00

abstract：:1. Cofilin is an essential actin-regulating protein widely distributed in all eucaryotes. The structure and function of cofilin are conserved during evolution. 2. Cofilin depolymerizes F-actin in vitro at alkaline pH and severs F-actin in vitro at pH lower than 7.3. Overexpression of cofilin in viable cells induced bu...

journal_title：Cell structure and function

authors： Yahara I,Aizawa H,Moriyama K,Iida K,Yonezawa N,Nishida E,Hatanaka H,Inagaki F

更新日期：1996-10-01 00:00:00

abstract：:The putative ionophoretic action of phosphatidic acid or arachidonic acid metabolites for Ca2+ has offered an attractive explanation for stimulation-coupled mobilization of cytoplasmic Ca2+. We have examined the effects of Ca2+ ionophore and long-chain unsaturated fatty acids on the translocation of Ca2+ across the li...

journal_title：Cell structure and function

authors： Utsumi K,Nobori K,Miyahara M

更新日期：1985-06-01 00:00:00

abstract：:Immunoblots of proteins extracted from the skin of a small viviparous fish (Xiphophorus) showed that a monoclonal antibody against human urokinase recognizes multiple molecular weight species of antigens. The immunoaffinity-purified antigens had serine-protease activity for the hydrolysis of a chromogenic substrate an...

journal_title：Cell structure and function

authors： Takahashi K,Wakamatsu Y,Ozato K,Wakayama Y

更新日期：1987-02-01 00:00:00