Abstract:
:Here we report a new method of isolating epidermal desmosomes from Xenopus laevis, and a major constituent of desmosomes designated as Xenopus desmogleins (XDsg). Isolation of desmosomes from Xenopus laevis epidermis was carried out by a two step-incubation with different concentrations of NP-40. After discontinuous sucrose gradient centrifugation at 30,000 g for 60 min, a pure desmosomal fraction was obtained at 30%/40% interface. In the SDS-PAGE of isolated desmosomes, at least 12 bands (XDB1 to XDB12) were observed over a 75 kD region. Among them, three bands (XDB3, XDB7, XDB8; estimated MW 175, 124, and 112 kD respectively) were recognized as glycoproteins based on ConA binding. Monospecific polyclonal antibody against XDB3 cross-reacted with bovine Dsgs and vis-a-vis anti-bovine Dsgs with XDB3. By contrast, monospecific antibody against bovine Dsc a/b did not cross-react with either XDB7 or XDB8. Heterogeneous molecular constituents of desmosomal adhesion molecule, which have been observed among different bovine tissues, were confirmed in a phylogenetically different animal, Xenopus laevis. Combined results with other evidence could suggest an alternative system for desmosome-mediated cell adhesion.
journal_name
Cell Struct Functjournal_title
Cell structure and functionauthors
Ohga R,Shida M,Shida Hdoi
10.1247/csf.29.17keywords:
subject
Has Abstractpub_date
2004-02-01 00:00:00pages
17-26issue
1eissn
0386-7196issn
1347-3700journal_volume
29pub_type
杂志文章abstract::Autophagy is a starvation response in eukaryotes by which the cell delivers cytoplasmic components to the vacuole for degradation, and is mediated by a double membrane structure called the autophagosome. We have previously proposed that the specific combination of COPII like components, including Sec24p, is required f...
journal_title:Cell structure and function
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journal_title:Cell structure and function
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journal_title:Cell structure and function
pub_type: 杂志文章
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journal_title:Cell structure and function
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journal_title:Cell structure and function
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journal_title:Cell structure and function
pub_type: 杂志文章,评审
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更新日期:1997-02-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.17.77
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journal_title:Cell structure and function
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journal_title:Cell structure and function
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journal_title:Cell structure and function
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journal_title:Cell structure and function
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journal_title:Cell structure and function
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journal_title:Cell structure and function
pub_type: 杂志文章,评审
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