Luminal domain of ATF6 alone is sufficient for sensing endoplasmic reticulum stress and subsequent transport to the Golgi apparatus.

abstract：:Mouse embryonic stem (mES) cells can be maintained in undifferentiated state in the presence of a cytokine, leukemia inhibitory factor (LIF). Many investigators found that STAT3 activation is important for the maintenance of pluripotency by LIF. However, the downstream pathways of STAT3 activation are still unknown. T...

journal_title：Cell structure and function

authors： Ko SY,Kang HY,Lee HS,Han SY,Hong SH

更新日期：2006-01-01 00:00:00

abstract：:Distribution of peanut agglutinin binding sites was studied histologically with horseradish peroxidase labelled and fluorescein isothiocyanate labelled peanut agglutinin in terms of cell differentiation in rat lymphatic organs, (thymus, spleen, lymph nodes). In this study, alcohol-fixed paraffin-embedded tissue sectio...

journal_title：Cell structure and function

authors： Kabir A,Watanabe M,Takeda Z,Kizaki T,Urano Y

更新日期：1983-03-01 00:00:00

abstract：:Cells of Euglena gracilis Klebs var. bacillaris Cori mutant W3BUL grown in darkness on Hutner's pH 3.5 medium without agitation accumulate wax ester. These cells have undeveloped proplastid remnants characteristic of this mutant. If these cells are transferred to an inorganic medium and bubbled with 2-3% CO2 in air, t...

journal_title：Cell structure and function

authors： Osafune T,Schiff JA,Hase E

更新日期：1987-10-01 00:00:00

abstract：:CD9 and CD63 belong to a tetramembrane-spanning glycoprotein family called tetraspanin, and are involved in a wide variety of cellular processes, but the structure-function relationship of this family of proteins has yet to be clarified. CD9 associates with diphtheria toxin receptor (DTR), which is identical to the me...

journal_title：Cell structure and function

authors： Ryu F,Takahashi T,Nakamura K,Takahashi Y,Kobayashi T,Shida S,Kameyama T,Mekada E

更新日期：2000-10-01 00:00:00

abstract：:Genomics and proteomics studies in Chlamydomonas have revealed that an axoneme is composed of 200-600 types of proteins, including uncharacterized proteins collectively named flagellar-associated proteins (FAPs). Nine FAPs contain the EF-hand motif; however, they have not yet been well characterized. To find component...

journal_title：Cell structure and function

authors： Kirima J,Oiwa K

更新日期：2018-02-16 00:00:00

abstract：:To construct recombinant retroviruses with only a single active promoter, we introduced point mutations into the TATA box region of the 3'-LTR, and successfully obtained high-titer virus with sufficient self-inactivating activity. However, the viral titer could not be determined by the number of G418 resistant colonie...

journal_title：Cell structure and function

authors： Miyao Y,Shimizu K,Tamura M,Yamada M,Tamura K,Nakahira K,Kuriyama S,Hayakawa T,Ikenaka K

更新日期：1995-04-01 00:00:00

abstract：:We have previously shown that mouse L.P3 cells secrete fibronectin and a novel protein, gelatin-non-binding and heparin-binding cell-adhesive protein (GNCP). Here, we screened and characterized cell-adhesive proteins in the conditioned media (CM's) of a series of cell lines growing in a protein- and lipid-free synthet...

journal_title：Cell structure and function

authors： Morita H,Yasumitsu H,Watanabe Y,Miyazaki K,Umeda M

更新日期：1993-02-01 00:00:00

abstract：:Upon tryptic digestion of synaptosomes, ATPase activities decreased in the order of Cl(-)-ATPase greater than or equal to Na+,K+-ATPase greater than anion-insensitive Mg2+-ATPase. Upon synaptosome treatment with hypotonic solution, Cl(-)-ATPase or anion-insensitive Mg2+-ATPase was slightly inactivated, while Na+,K+-AT...

journal_title：Cell structure and function

authors： Kunugi-Uehara Y,Hashimoto Y,Ogura C,Inagaki C,Nishino T

更新日期：1988-04-01 00:00:00

abstract：:Tenascin-C is an oligomeric glycoprotein of the extracellular matrix that has been found to have both adhesive and anti-adhesive properties for cells. Recent elucidation of the two major TNC splice variants (320 kDa and 220 kDa) has shed light on the possibility of varying functions of the molecule based on its splici...

journal_title：Cell structure and function

authors： Ghert MA,Qi WN,Erickson HP,Block JA,Scully SP

更新日期：2001-06-01 00:00:00

abstract：:Hepatic stellate cells (HSCs) were changed in their morphology, proliferative activity, and functions by culturing on type I collagen gel, as compared to the culture on polystyrene surface. HSCs have been found to produce extracellular matrix components and matrix metalloproteinases (MMPs). In this study, we have asse...

journal_title：Cell structure and function

authors： Li YL,Sato M,Kojima N,Miura M,Senoo H

更新日期：1999-10-01 00:00:00

abstract：:Contact reaction is the morphological expression of allogeneic recognition by hemocytes in the ascidian, Halocynthia roretzi; namely they undergo an exocytotic burst upon contact with non-self (allogeneic) hemocytes in vitro. We have found that contact reaction is accompanied by a burst of cyanide-insensitive oxygen c...

journal_title：Cell structure and function

authors： Akita N,Hoshi M

更新日期：1995-02-01 00:00:00

abstract：:The involvement of actin filaments from the host cell on the process of invasion of trypomastigote forms of Trypanosma cruzi was analyzed in seven different cell lines. Prior incubation of all cell lines with cytochalasin D, under conditions which interfere with actin filaments, markedly inhibited parasite internaliza...

journal_title：Cell structure and function

authors： Rosestolato CT,Dutra Jda M,De Souza W,de Carvalho TM

更新日期：2002-04-01 00:00:00

abstract：:Under unfavorable conditions for growth, haploid myxoamoebae of Physarum polycephalum retracted their pseudopodia and changed their cell shape into disk-like form, after which they constructed the cell walls to form microcysts. These morphological changes of haploid cells were associated with changes in intracellular ...

journal_title：Cell structure and function

authors： Shimada Y,Kasakura T,Yokota M,Miyata Y,Murofushi H,Sakai H,Yahara I,Murakami-Murofushi K

更新日期：1992-10-01 00:00:00

abstract：:High voltage electric pulses, 1000 V/cm, were found to induce cell fusion efficiently when delivered to cells growing in a monolayer culture. The maximum yield of fused cells was about 30% of cells remaining after treatment. Colonies of interspecies hybrid cells between mouse and others also appeared with a frequency ...

journal_title：Cell structure and function

authors： Ishizaki K,Chang HR,Eguchi T,Ikenaga M

更新日期：1989-04-01 00:00:00

abstract：:Synapsin I, one of the major synaptic proteins, is thought to associate with synaptic vesicles and to play a regulatory role in neurotransmitter release. In mature neurons, it is concentrated almost exclusively in presynaptic nerve endings. Here, we studied the subcellular localization of synapsin I during the develop...

journal_title：Cell structure and function

authors： Harada A,Sobue K,Hirokawa N

更新日期：1990-12-01 00:00:00

abstract：:In the mitotic sea urchin egg, the spindle microtubules were composed of different tubulin isotypes from those of astral microtubules using monoclonal antibodies [Oka et al. (1990) Cell Motil. Cytoskeleton, 16, 239-250]. Three of the antibodies, D2D6, DM1B, and YL1/2, were specific for spindle microtubules, astral mic...

journal_title：Cell structure and function

authors： Oka MT,Arai T,Hamaguchi Y

更新日期：1991-04-01 00:00:00

abstract：:Two monoclonal antibodies against alpha-tubulin (YL1/2 and D2D6) were microinjected into the egg of the sand dollar Clypeaster japonicus, and their effects on cleavage of the egg were investigated. They had already been shown by immunoblotting to react specifically with egg tubulin and by immunofluorescence to stain t...

journal_title：Cell structure and function

authors： Oka MT,Arai T,Hamaguchi Y

更新日期：1990-12-01 00:00:00

abstract：:Caffeine has been shown to enhance the lethal effect of DNA-damaging agents in mammalian cells, and the potentiation by caffeine of this effect is generally interpreted as the result of inhibition by caffeine of the repair of damaged DNA. However, the mechanism by which caffeine enhances the lethal effect of DNA-damag...

journal_title：Cell structure and function

authors： Ishida R,Kozaki M,Takahashi T

更新日期：1985-12-01 00:00:00

abstract：:Interleukin 2 (IL-2) initiated proliferation of the cells of T-enriched population stimulated with non-mitogenic dose of concanavalin A (Con A), whereas it could not induce proliferation of the cells treated with non-mitogenic lectin wheat germ agglutinin (WGA). Use of monoclonal antibody (MAb) directed against interl...

journal_title：Cell structure and function

authors： Komada H,Nakabayashi H,Hara M,Hara M,Takahashi T,Morita J,Yoshida T,Takanari H,Izutsu K

更新日期：1989-04-01 00:00:00

abstract：:When human erythroleukemia cells (K562) were exposed to phorbol-12-myristate 13-acetate (PMA), phosphorylation of transferrin receptors was enhanced 5-fold with 10(-7) M PMA and 7-fold with 10(-6) M PMA, but not with 4 alpha-phorbol (5 X 10(-7) M). Stimulation took place in serine residues in the cytoplasmic domain of...

journal_title：Cell structure and function

authors： Kohno H,Taketani S,Tokunaga R

更新日期：1985-06-01 00:00:00

abstract：:Embryonic stem (ES) cells are a pluripotent cell line derived from the inner cell mass of the mouse blastocyst. The mRNA levels of several growth-related genes were examined in ES cells by Northern blot analysis under several different growth conditions. In a coculture of ES cells with the mouse fibroblast cell line, ...

journal_title：Cell structure and function

authors： Katsube K,Shimizu N

更新日期：1991-10-01 00:00:00

abstract：:Myofibril assembly requires the cell to join diverse components, correctly oriented to the rest of the cell. Titin, a huge elastic protein with a role in myogenesis, assembles during translation in vivo and may require spatially organized mRNA to allow assembly. By immunofluorescence, we examined titin and myosin prot...

journal_title：Cell structure and function

authors： Fulton AB,Alftine C

更新日期：1997-02-01 00:00:00

abstract：:tsJT60 is a nonlethal temperature-sensitive (ts) mutant of a Fischer rat cell line (3Y1) classified as a G0 mutant; i.e., the ts defect is not expressed within the cell growth cycle but is expressed only between the G0 and S phase. tsJT60 clones transformed with oncogenes such as adenovirus E1A, polyoma large T, polyo...

journal_title：Cell structure and function

authors： Tai YY,Ninomiya-Tsuji J,Furuoku K,Ogawa N,Ishibashi S,Shiroki K,Segawa K,Tsuchida N,Shibuya M,Ide T

更新日期：1990-12-01 00:00:00

abstract：:An immunocytochemical method using a specific monoclonal antibody was employed to detect DNA polymerase alpha in Drosophila melanogaster embryos during the first 13 nuclear division cycles after fertilization. The anti-DNA polymerase alpha antibody stained the ooplasm of the unfertilized egg, indicating that DNA polym...

journal_title：Cell structure and function

authors： Yamaguchi M,Kuroda K,Hirose F,Matsukage A

更新日期：1992-04-01 00:00:00

abstract：:Phosphorylation of regulatory light chain (RMLC) of myosin II at Ser19/Thr18 is likely to play important roles in controlling the morphological changes seen during cell division of cultured mammalian cells. Phosphorylation of RMLC regulates the activity of myosin II, an essntial motor for cytokinesis, and phosphorylat...

journal_title：Cell structure and function

authors： Matsumura F,Totsukawa G,Yamakita Y,Yamashiro S

更新日期：2001-12-01 00:00:00

abstract：:tsJT60, a temperature-sensitive (ts) G0-mutant cell line from a Fischer rat, grows normally in the exponential growth phase at 34 degrees C and 39.5 degrees C, but when stimulated with fetal bovine serum (FBS), from the G0 phase they reenter the S phase at 34 degrees C but not at 39.5 degrees C. The ts-block was bypas...

journal_title：Cell structure and function

authors： Ninomiya-Tsuji J,Nakahara Y,Ito C,Akiyama T,Ishibashi S,Ide T

更新日期：1987-10-01 00:00:00

abstract：:Two-dimensional gel electrophoresis revealed two protein spots, a (molecular weight 70,000, pI 4.6) and b (molecular weight 69,000, pI 4.4) in PC-12 cells (rat pheochromocytoma cells). When the cells were induced to differentiate with nerve growth factor, the amount of protein in spot a, and later spot b increased wit...

journal_title：Cell structure and function

authors： Noguchi S,Muramatsu T,Koike T

更新日期：1987-02-01 00:00:00

abstract：:We constructed a fusion protein of the cell-binding domain of human fibronectin and human basic fibroblast growth factor, and prepared a polypeptide with both cell-adhesive activity and growth factor activity. A human gene fragment coding for basic fibroblast growth factor was amplified by the polymerase chain reactio...

journal_title：Cell structure and function

authors： Hashi H,Hatai M,Kimizuka F,Kato I,Yaoi Y

更新日期：1994-02-01 00:00:00

abstract：:G0-arrested human diploid fibroblasts, TIG-1, was stimulated to induce DNA synthesis by serum, epidermal growth factor (EGF), colchicine, colcemid, or 12-O-tetradecanoylphorbol-13-acetate (TPA). The induction of DNA synthesis was mediated by protein kinase C (PKC) when stimulated with TPA but not when stimulated with ...

journal_title：Cell structure and function

authors： Tsuji K,Ueno A,Ide T

更新日期：1991-02-01 00:00:00

abstract：:The Golgi stress response is a homeostatic mechanism that controls the capacity of the Golgi apparatus in accordance with cellular demands. When the capacity of the Golgi apparatus becomes insufficient (Golgi stress), transcription levels of Golgi-related genes encoding glycosylation enzymes, a Golgi structural protei...

journal_title：Cell structure and function

authors： Taniguchi M,Sasaki-Osugi K,Oku M,Sawaguchi S,Tanakura S,Kawai Y,Wakabayashi S,Yoshida H

更新日期：2016-07-30 00:00:00