Abstract:
:Distribution of peanut agglutinin binding sites was studied histologically with horseradish peroxidase labelled and fluorescein isothiocyanate labelled peanut agglutinin in terms of cell differentiation in rat lymphatic organs, (thymus, spleen, lymph nodes). In this study, alcohol-fixed paraffin-embedded tissue sections were used and proved to be useful for the histochemical study with peanut agglutinin. In the germinal center of the lymph node, cells were weakly positive for peanut agglutinin binding sites but not in the mantle zone of the lymph follicle. In the thymus, the cortical thymocytes were weakly positive for peanut agglutinin binding sites but not in the medulla. In the spleen, some cells on the periphery of the white pulp were weakly positive for PNA binding sites but cells around the central artery were not positive. Large cells with granular cytoplasma around the sinus of the spleen and lymph node, thought to be fixed macrophages, were strongly positive for PNA binding sites.
journal_name
Cell Struct Functjournal_title
Cell structure and functionauthors
Kabir A,Watanabe M,Takeda Z,Kizaki T,Urano Ydoi
10.1247/csf.8.29subject
Has Abstractpub_date
1983-03-01 00:00:00pages
29-34issue
1eissn
0386-7196issn
1347-3700journal_volume
8pub_type
杂志文章abstract::The in vivo structure of the smooth endoplasmic reticulum (ER) was visualized in rat and mouse cerebellar Purkinje cells by using quick-freezing techniques followed by freeze-substitution for ultrathin-sectioning or freeze-fracturing and deep-etching for replicas. High magnification electron microscopy of the ultrathi...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.23.373
更新日期:1998-12-01 00:00:00
abstract::The localization of mRNA in neuronal dendrites plays a role in both locally and temporally regulated protein synthesis, which is required for certain forms of synaptic plasticity. RNA granules constitute a dendritic mRNA transport machinery in neurons, which move along microtubules. RNA granules contain densely packed...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
doi:10.1247/csf.30.51
更新日期:2005-01-01 00:00:00
abstract::Myofibril assembly requires the cell to join diverse components, correctly oriented to the rest of the cell. Titin, a huge elastic protein with a role in myogenesis, assembles during translation in vivo and may require spatially organized mRNA to allow assembly. By immunofluorescence, we examined titin and myosin prot...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.22.51
更新日期:1997-02-01 00:00:00
abstract::One of the proposed roles of sarcoglycan is to stabilize dystrophin glycoprotein complexes in muscle sarcolemma. Involvement in signal transduction has also been proposed and abnormalities in some sarcoglycan genes are known to be responsible for muscular dystrophy. While characterization of sarcoglycans in muscle has...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.06025
更新日期:2006-01-01 00:00:00
abstract::Vascular endothelial cells possess antithrombotic properties, which are determined by the balance between plasminogen activators (PAs) and PA inhibitors (PAls). A cell line, TKM-33, has been established and cloned from human umbilical vein endothelial cells, was previously reported to produce a large amount of urokina...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.24.71
更新日期:1999-04-01 00:00:00
abstract::Marvelous background rejection in total internal reflection fluorescence microscopy (TIR-FM) has made it possible to visualize single-fluorophores in living cells. Cell signaling proteins including peptide hormones, membrane receptors, small G proteins, cytoplasmic kinases as well as small signaling compounds have bee...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
doi:10.1247/csf.27.357
更新日期:2002-10-01 00:00:00
abstract::Two types of DNA ligase, I and II, have been purified approximately 4,000-fold from mouse testes and 500-fold from nuclei of mouse spermatocytes. DNA ligase I and II consisted of single polypeptides with molecular weights of 95,000 and 65,000, respectively, according to the estimation by SDS-polyacrylamide gel electro...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.15.67
更新日期:1990-02-01 00:00:00
abstract::To investigate the mechanism of myogenic differentiation, we are using quail myoblast cells (QM cells) transformed with a temperature-sensitive mutant of Rous sarcoma virus (ts-RSV), termed QM-RSV cells. At 35.5 degrees C, a permissive temperature for RSV, QM-RSV cells repeatedly proliferate without differentiation, b...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.21.17
更新日期:1996-02-01 00:00:00
abstract::DNA synthesis of WF-1 fibroblasts derived from a patient with Werner's syndrome was stimulated by fetal calf serum and adult human serum but not by various mitogens including epidermal growth factor, platelet-derived growth factor (PDGF), fibroblast growth factor, insulin and 12-O-tetradecanoylphorbol-13-acetate (TPA)...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.13.471
更新日期:1988-12-01 00:00:00
abstract::The change in distribution of centrosomal phosphoproteins was examined in sea urchin eggs from fertilization to the first cleavage by immunofluorescence staining with the anti-phosphoprotein antibodies, MPM-1 and MPM-2. The antibodies reacted with female pronuclei in unfertilized eggs as well as centriolar complexes l...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.15.13
更新日期:1990-02-01 00:00:00
abstract::Checkpoint kinase 1 (Chk1) is a conserved protein kinase central to the cell-cycle checkpoint during DNA damage response (DDR). Until recently, ATR, a protein kinase activated in response to DNA damage or stalled replication, has been considered as the sole regulator of Chk1. Recent progress, however, has led to the i...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
doi:10.1247/csf.14017
更新日期:2015-01-01 00:00:00
abstract::Here, we would like to point out important milestones in the study of nuclear radial positioning and gene expression during differentiation processes. In addition, changes in the histone signature that significantly precede various differentiation pathways are reviewed. We address the regulatory functions of chromatin...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
doi:10.1247/csf.09021
更新日期:2010-01-01 00:00:00
abstract::Recent studies have revealed various biological functions for repetitive sequences, which make up about half of the human genome. One such sequence, major satellites, which are tandem repetitive sequences adjacent to the centromere, have been shown to be a kinetochore component that plays a role in the formation and f...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.12009
更新日期:2012-01-01 00:00:00
abstract::It has been shown that in PC12 and its subclone PC12h treatment of the cells with nerve growth factor (NGF) induces a selective decrease in the incorporation of radioactive phosphate into a 100,000-dalton protein, designated in an earlier study as Nsp100, in the subsequent phosphorylation of soluble extracts from cell...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.14.87
更新日期:1989-02-01 00:00:00
abstract::All trans retinoic acid (ATRA), a differentiation inducer for human myeloid NB4 cells, induced accumulation of lipid droplet as determined by positivity of Nile Red and Oil Red O in this cell line. Granulocyte colony-stimulating factor (G-CSF), although not having detectable effect by itself, exerted the additive effe...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.28.487
更新日期:2003-10-01 00:00:00
abstract::The precise control of spindle microtubule (MT) dynamics is essential for chromosome capture and alignment. Kif18A/kinesin-8, an essential regulator of kinetochore MT dynamics, accumulates at its plus-ends in metaphase but not prometaphase cells. The underlying mechanism of time-dependent and kinetochore MT-specific p...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.11032
更新日期:2011-01-01 00:00:00
abstract::The Golgi stress response is a homeostatic mechanism that controls the capacity of the Golgi apparatus in accordance with cellular demands. When the capacity of the Golgi apparatus becomes insufficient (Golgi stress), transcription levels of Golgi-related genes encoding glycosylation enzymes, a Golgi structural protei...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.16005
更新日期:2016-07-30 00:00:00
abstract::Many studies on in vitro transformation of human cells indicate that the cells must be immortalized before they can be neoplastically transformed, indicating that immortalization is a critical step in multistep neoplastic transformation of human cells. We immortalized three human cell lines by repeated treatment with ...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.21.111
更新日期:1996-04-01 00:00:00
abstract::alpha-1,3-Fucosyltransferase (Fuc T IV) cDNA was placed under the control of beta-actin, cytomegalovirus enhancer/promoter and transfected into embryonic stem cells. The transfected cell clones with integrated cDNA (positive clones) differentiated more efficiently into myocardial cell clones without integrated cDNA (n...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.22.247
更新日期:1997-04-01 00:00:00
abstract::Tobacco BY-2 cells were synchronized by an aphidicolin treatment, and their beta-tubulin isoforms and their mRNA were analyzed by Western, Northern and dot blottings. The relative ratio of the beta-tubulin isoforms changed with the progress of cell cycle stage. By Northern blot hybridization of poly(A)+RNAs with a clo...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.16.489
更新日期:1991-12-01 00:00:00
abstract::In this study, we measured hydrogen peroxide (H2O2) release as one of the functions of mature eosinophils, and utilized it as a quantitative index. We demonstrated that 1) the human eosinophilic leukemia cell line, EoL-1, did not release H2O2 when stimulated with phorbol myristate acetate (PMA), but after culturing wi...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.18.125
更新日期:1993-04-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.24.255
更新日期:1999-10-01 00:00:00
abstract::In yeast Saccharomyces cerevisiae cells, some aberrant multimembrane-spanning proteins are not transported to the cell surface but form and are accumulated in endoplasmic reticulum (ER)-derived subcompartments, known as the ER-associated compartments (ERACs), which are observed as puncta under fluorescence microscopy....
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.19023
更新日期:2019-11-23 00:00:00
abstract::Effects of kinase inhibitors on the preprophase band of microtubules in onion (Allium cepa L.) root tip cells were examined. Bundled microtubules in preprophase bands were dispersed on the cell cortex when onion seedlings were incubated with 2.5-5.0 mM 6-dimethylaminopurine. Fifteen min was enough for the bundled micr...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.24.419
更新日期:1999-10-01 00:00:00
abstract::We investigated the assembly of the first sarcomeres in chicken embryos by confocal microscopy of immunofluorescently stained whole mount rudiments of early chicken hearts isolated around the onset of beating. In embryos with merely 9 somites, myomesin was found to be present in a cross striated pattern, indicating th...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.22.139
更新日期:1997-02-01 00:00:00
abstract::Here we describe the design and synthesis of a bifunctional two-photon fluorescence probe, N,N'-dimethyl-4,4'-(biphenyl-2,1-ethenediyl)dipyridinium hexafluorophosphate (BP6). HeLa, Hek293, and Paramecium caudatum cells were stained with BP6. BP6 accumulated on the mitochondria of all three cell types when the mitocho...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.14006
更新日期:2014-01-01 00:00:00
abstract::Previously, we serially transplanted tumors that produced colony-stimulating factor (CSF) into nude mice, who developed marked granulocytosis along with tumor growth; their leukocyte counts reaching approximately one million per cu mm. The numbers of CFU-GM, CFU-E, CFU-Meg, CFU-S and BFU-E were increased in nude mice ...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.12.273
更新日期:1987-06-01 00:00:00
abstract::The substance in sperm which induces the activation of eggs at fertilization has yet to be elucidated. Osawa et al. reported that the soluble extract of sperm (spex) causes inositol 1,4,5-trisphosphate (IP3)-induced Ca2+ release (IICR) in fertilized eggs of sea urchins when externally applied [Osawa et al., (1992). Zo...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.19.73
更新日期:1994-04-01 00:00:00
abstract::Interleukin 2 (IL-2) initiated proliferation of the cells of T-enriched population stimulated with non-mitogenic dose of concanavalin A (Con A), whereas it could not induce proliferation of the cells treated with non-mitogenic lectin wheat germ agglutinin (WGA). Use of monoclonal antibody (MAb) directed against interl...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.14.193
更新日期:1989-04-01 00:00:00
abstract::Cytokeratin 8 (CK8) and cytokeratin 19 (CK19) is a specific cytoskeletal component of simple epithelia, including bronchial epithelial cells. We hypothesized that CK8 or CK19 released from epithelial cells may bind to and cause damage to extracellular matrixes through binding of anti-CK8 or anti-CK19 autoantibodies. I...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.25.171
更新日期:2000-06-01 00:00:00