Abstract:
:Upon tryptic digestion of synaptosomes, ATPase activities decreased in the order of Cl(-)-ATPase greater than or equal to Na+,K+-ATPase greater than anion-insensitive Mg2+-ATPase. Upon synaptosome treatment with hypotonic solution, Cl(-)-ATPase or anion-insensitive Mg2+-ATPase was slightly inactivated, while Na+,K+-ATPase underwent a much larger degree of inactivation. ATP-Mg inhibited the ATPase digestion in the hypotonic-solution-treated synaptosomes in a concentration-dependent manner, but not in the untreated synaptosomes. These results suggest that trypsin-digestible site of Cl(-)-ATPase are present on both sides of the synaptosomal plasma membrane, and the ATP-Mg binding site of the enzyme is located on the inner surface of the membrane.
journal_name
Cell Struct Functjournal_title
Cell structure and functionauthors
Kunugi-Uehara Y,Hashimoto Y,Ogura C,Inagaki C,Nishino Tdoi
10.1247/csf.13.105subject
Has Abstractpub_date
1988-04-01 00:00:00pages
105-11issue
2eissn
0386-7196issn
1347-3700journal_volume
13pub_type
杂志文章abstract::Myofibril assembly requires the cell to join diverse components, correctly oriented to the rest of the cell. Titin, a huge elastic protein with a role in myogenesis, assembles during translation in vivo and may require spatially organized mRNA to allow assembly. By immunofluorescence, we examined titin and myosin prot...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.22.51
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abstract::Histone chaperones are a group of histone-binding proteins that facilitate the assembly of nucleosomes, the fundamental structural units of chromatin in eukaryotes. In nucleosome assembly, deposition of a histone H3-H4 tetramer onto DNA is the first and critical step, which is mediated by the histone chaperones HIRA a...
journal_title:Cell structure and function
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abstract::Marvelous background rejection in total internal reflection fluorescence microscopy (TIR-FM) has made it possible to visualize single-fluorophores in living cells. Cell signaling proteins including peptide hormones, membrane receptors, small G proteins, cytoplasmic kinases as well as small signaling compounds have bee...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
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更新日期:2002-10-01 00:00:00
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journal_title:Cell structure and function
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journal_title:Cell structure and function
pub_type: 杂志文章,评审
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更新日期:2000-08-01 00:00:00
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journal_title:Cell structure and function
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journal_title:Cell structure and function
pub_type: 杂志文章
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更新日期:1997-06-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
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更新日期:2014-01-01 00:00:00
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journal_title:Cell structure and function
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journal_title:Cell structure and function
pub_type: 杂志文章
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更新日期:1987-10-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.24.419
更新日期:1999-10-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
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更新日期:1997-10-01 00:00:00
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journal_title:Cell structure and function
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journal_title:Cell structure and function
pub_type: 杂志文章
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更新日期:1987-10-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.28.487
更新日期:2003-10-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.20.345
更新日期:1995-10-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.12.539
更新日期:1987-12-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
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journal_title:Cell structure and function
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更新日期:1993-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1985-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1990-12-01 00:00:00
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pub_type: 杂志文章
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journal_title:Cell structure and function
pub_type: 杂志文章
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journal_title:Cell structure and function
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