Abstract:
:The role of microtubule associated proteins (MAPs) on the dynamic instability of microtubules was examined under a dark-field microscope using bovine brain tubulin purified by DEAE-Sepharose column chromatography. In the absence of MAPs, the transition from the shortening phase to the growing phase (the rescue) occurred rarely both in self-assembled microtubules and seeded ones, especially at the plus end. Even under the conditions unfavorable to stabilize microtubule, the addition of a small amount of crude MAPs or purified microtubule associated protein 2 (MAPs) to the microtubules allowed them to undergo the rescue. At increased concentrations of MAPs or MAP2, both the length change required for a rescue during shortening phase ("shortening length") and for a catastrophe (transition from the growing to the shortening phase) ("growth length") decreased. Under these conditions, the rescue often occurred at the same site where previous rescues occurred. Distribution of immunofluorescent MAP2 antibodies along individual microtubules showed that MAP2 molecules bound onto microtubules by forming discrete clusters. The number of MAP2 molecules per cluster was estimated to be between 25 and 60. Because both the "shortening length" and the distance between MAP2 clusters in a microtubule decreased with increased MAPs concentration, we suggest that the MAP2 clusters may form the specific site at which the shortening of the microtubule readily stops. MAP2 possibly regulates the dynamic instability by stopping the shortening, which is a prerequisite for the rescue.
journal_name
Cell Struct Functjournal_title
Cell structure and functionauthors
Itoh TJ,Hotani Hdoi
10.1247/csf.19.279subject
Has Abstractpub_date
1994-10-01 00:00:00pages
279-90issue
5eissn
0386-7196issn
1347-3700journal_volume
19pub_type
杂志文章abstract::Cytokeratin 8 (CK8) and cytokeratin 19 (CK19) is a specific cytoskeletal component of simple epithelia, including bronchial epithelial cells. We hypothesized that CK8 or CK19 released from epithelial cells may bind to and cause damage to extracellular matrixes through binding of anti-CK8 or anti-CK19 autoantibodies. I...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.25.171
更新日期:2000-06-01 00:00:00
abstract::DNA-dependent protein kinase (DNA-PK), consisting of the 470-kDa catalytic component (DNA-PKcs) and the DNA-binding regulatory component Ku protein (p70/p80), catalyzes phosphorylation of a variety of DNA replication/transcription/repair factors in the presence of double-stranded DNA. In the resting states of human pe...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.22.585
更新日期:1997-12-01 00:00:00
abstract::Microtubules are intrinsically dynamic polymers. Two kinds of dynamic behaviors, dynamic instability and treadmilling, are important for microtubule function in cells. Both dynamic behaviors appear to be tightly regulated, but the cellular molecules and the mechanisms responsible for the regulation remain largely unex...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
doi:10.1247/csf.24.329
更新日期:1999-10-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.11032
更新日期:2011-01-01 00:00:00
abstract::The effects of heavy ion microbeams on muscle fibers isolated from mouse skeletal muscles were examined by electron microscopy. The plasma membranes of heavy ion beam-irradiated areas of muscle fibers showed irregular protrusions and invaginations. In the cytoplasm, an irregular distribution of microfilaments was foun...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.06038
更新日期:2007-01-01 00:00:00
abstract::The Acanthamoeba myosin-IA and myosin-IB molecular motors bind to membranes, so they may produce the force to move organelles and membranes along actin filaments. We have determined the rate constants for the actin-activated myosin-I ATPase by pre-steady state kinetic analysis. ATP binds rapidly to myosin-I and dissoc...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
doi:10.1247/csf.21.351
更新日期:1996-10-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.12.443
更新日期:1987-10-01 00:00:00
abstract::We earlier discovered a novel 40-kDa protein (hsp40) induced by heat shock and other stresses in mammalian and avian cells. In this report, we purified the hsp40 in HeLa cells, using modified two-dimensional gel electrophoresis, and determined the amino terminal amino acid sequence of this protein. The hsp40 is homolo...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.17.77
更新日期:1992-02-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.26.235
更新日期:2001-08-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.28.505
更新日期:2003-12-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.10.339
更新日期:1985-12-01 00:00:00
abstract::Analysis of the amino acid sequence in protein (deviation analysis) suggests that the binding between trypsin and the enveloped virus is the first step of their interaction, which occurs in a specified configuration. It is possible that the distance between their active sites is important for the viral sensitivity to ...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.14.721
更新日期:1989-12-01 00:00:00
abstract::A voltage-dependent and Ca2(+)-activated cation channel recently found in the vacuolar membrane of the yeast Saccharomyces cerevisiae was incorporated into planar lipid bilayers and further characterized in macroscopic and single channel levels. Single channel conductances for various cations were in the order: NH4+ g...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.14.659
更新日期:1989-12-01 00:00:00
abstract::Interleukin 2 (IL-2) initiated proliferation of the cells of T-enriched population stimulated with non-mitogenic dose of concanavalin A (Con A), whereas it could not induce proliferation of the cells treated with non-mitogenic lectin wheat germ agglutinin (WGA). Use of monoclonal antibody (MAb) directed against interl...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.14.193
更新日期:1989-04-01 00:00:00
abstract::1. Cofilin is an essential actin-regulating protein widely distributed in all eucaryotes. The structure and function of cofilin are conserved during evolution. 2. Cofilin depolymerizes F-actin in vitro at alkaline pH and severs F-actin in vitro at pH lower than 7.3. Overexpression of cofilin in viable cells induced bu...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
doi:10.1247/csf.21.421
更新日期:1996-10-01 00:00:00
abstract::The unique Physarum lysophosphatidic acid, PHYLPA, having a cyclopropane in the fatty acid moiety and a cyclic phosphate at C-2 and C-3 positions of the glycerol, inhibited proliferation of human fibroblast cells, TIG-3 and TIG-7, which were cultured in a chemically defined (serum-free) medium. The cells at S- and M-p...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.18.363
更新日期:1993-10-01 00:00:00
abstract::In view of the importance of vitamin A in the human immune system and the central role of interleukin-2 (IL-2) in the proliferation of T-lymphocytes, we examined the effects of all-trans-retinoic acid (ATRA) on the protein and gene expression of IL-2 in the human T-cell line HUT-78 when stimulated with either 12-O-tet...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.07020
更新日期:2008-01-01 00:00:00
abstract::Thyroid follicles in vivo are embedded in extracellular matrix (ECM). The composing epithelial cells are in close contact with ECM at the basal side. To examine cell-to-ECM interactions, we studied adhesion, proliferation and differentiation of porcine follicle cells monolayer-cultured on type I and IV collagen, fibro...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.20.345
更新日期:1995-10-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.10.271
更新日期:1985-09-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.23.291
更新日期:1998-10-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.06013
更新日期:2006-01-01 00:00:00
abstract::NBT-II cells on collagen-coated substrates move rapidly and persistently, maintaining a semi-circular shape with a large lamellipodium, in a manner similar to fish keratocytes. The inhibitor of phospholipase D (PLD), n-butanol, completely blocked the migration and disturbed the characteristic localization of actin alo...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.07042
更新日期:2008-01-01 00:00:00
abstract::The relative fibrin-binding, fibrinolytic and fibrinogenolytic properties of single-chain pro-urokinase, an inactive proenzyme form of human urokinase purified from cultured human kidney cells, and urokinase were compared. The affinity of single-chain pro-urokinase for fibrin was much higher than that of urokinase. In...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.10.151
更新日期:1985-06-01 00:00:00
abstract::Two monoclonal antibodies against alpha-tubulin (YL1/2 and D2D6) were microinjected into the egg of the sand dollar Clypeaster japonicus, and their effects on cleavage of the egg were investigated. They had already been shown by immunoblotting to react specifically with egg tubulin and by immunofluorescence to stain t...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.15.373
更新日期:1990-12-01 00:00:00
abstract::To investigate the mechanism of myogenic differentiation, we are using quail myoblast cells (QM cells) transformed with a temperature-sensitive mutant of Rous sarcoma virus (ts-RSV), termed QM-RSV cells. At 35.5 degrees C, a permissive temperature for RSV, QM-RSV cells repeatedly proliferate without differentiation, b...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.21.17
更新日期:1996-02-01 00:00:00
abstract::Hepatic stellate cells (HSCs) were changed in their morphology, proliferative activity, and functions by culturing on type I collagen gel, as compared to the culture on polystyrene surface. HSCs have been found to produce extracellular matrix components and matrix metalloproteinases (MMPs). In this study, we have asse...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.24.255
更新日期:1999-10-01 00:00:00
abstract::In this study, we measured hydrogen peroxide (H2O2) release as one of the functions of mature eosinophils, and utilized it as a quantitative index. We demonstrated that 1) the human eosinophilic leukemia cell line, EoL-1, did not release H2O2 when stimulated with phorbol myristate acetate (PMA), but after culturing wi...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.18.125
更新日期:1993-04-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章,评审
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更新日期:2002-10-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.18.33
更新日期:1993-02-01 00:00:00
abstract::The localization of mRNA in neuronal dendrites plays a role in both locally and temporally regulated protein synthesis, which is required for certain forms of synaptic plasticity. RNA granules constitute a dendritic mRNA transport machinery in neurons, which move along microtubules. RNA granules contain densely packed...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
doi:10.1247/csf.30.51
更新日期:2005-01-01 00:00:00