Abstract:
:In view of the importance of vitamin A in the human immune system and the central role of interleukin-2 (IL-2) in the proliferation of T-lymphocytes, we examined the effects of all-trans-retinoic acid (ATRA) on the protein and gene expression of IL-2 in the human T-cell line HUT-78 when stimulated with either 12-O-tetradecanoylphorbol-13-acetate (TPA) or phytohemagglutinin (PHA). ATRA enhanced the production of IL-2 stimulated by TPA, but suppressed that stimulated by PHA. These findings were consistent with the results of a reverse transcription-polymerase chain reaction and quantitative real-time polymerase chain reaction examining IL-2 gene expression. ATRA augmented the gene expression of PKC-beta1 up-regulated by TPA and restored that suppressed by PHA but to below the control level. ATRA suppressed the c-fos gene expression up-regulated by PHA to a level of 36% of the control whereas it had no effect on the up-regulation by TPA. Since PKC- beta1 has been suggested to be important for the secretion and gene expression of IL-2 and since the activator protein-l binding site is present in the promoter of the IL-2 gene, these findings may explain the differences in ATRA's effects on TPA- and PHA-stimulated IL-2 expression. These results suggest that ATRA affects the production of IL-2 by T-lymphocytes in a stimulus-dependent manner.
journal_name
Cell Struct Functjournal_title
Cell structure and functionauthors
Goto S,Okada N,Kaneko A,Isemura Mdoi
10.1247/csf.07020subject
Has Abstractpub_date
2008-01-01 00:00:00pages
13-9issue
1eissn
0386-7196issn
1347-3700pii
JST.JSTAGE/csf/07020journal_volume
33pub_type
杂志文章abstract::To elucidate the cytotoxic mechanism of tumor necrosis factor (TNF), we isolated TNF-resistant sublines of L929 cells. As compared with L929 cells, TNF-resistant cells retained similar number and affinity of TNF-binding sites, and showed a similar growth rate. TNF stimulated arachidonate release from L929 cells, while...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.16.333
更新日期:1991-08-01 00:00:00
abstract::We have found that 1,9-Dideoxyforskolin (DDF) strongly inhibited the cell death induced by ricin, modeccin, Pseudomonas toxin, and diphtheria toxin in MDCK cells, suggesting that these protein toxins have a DDF-sensitive common pathway leading to cell death. However, no significant effect of forskolin on these toxins ...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.22.545
更新日期:1997-10-01 00:00:00
abstract::Human keratinocytes have been serially cultivated in low (0.015 mM) and high (1.8 mM) calcium containing medium. The calcium concentration of the growth medium significantly influenced the cell growth period in vitro. Cells grown in low calcium medium underwent 35-40 population doublings over 16-17 passages, while cel...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.12.539
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abstract::To evaluate the role of VAMP8/endobrevin in constitutive exocytosis, we have examined the exocytotic pathways of VAMP8 and human growth hormone, both GFP-tagged, by total internal reflection fluorescence microscopy (TIRF-M). Human GH-GFP and VAMP8-GFP were similarly expressed in small round vesicles and elongated tubu...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.09013
更新日期:2009-01-01 00:00:00
abstract::The amount of intact 40S rRNA precursor was followed by Northern hybridization in the course of the early embryogenesis of wild-type Xenopus laevis and its anucleolate mutant. The total amount of 40S rRNA precursor did not alter appreciably until the midblastula stage, decreased at the late blastula stage, and then in...
journal_title:Cell structure and function
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abstract::A method has been developed to isolate pure preparations of nuclei in high yield from commercially available viable rice embryos (germ), employing extraction with buffer solution containing glycerol (without detergent) and polyamine, followed by centrifugation on a 30% Percoll cushion. The intactness of the isolated n...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.17.87
更新日期:1992-04-01 00:00:00
abstract::The morphology and intramembranous particle (IMP) distribution of spermatozoa were investigated in four species of cyprinid fishes, Gnathopogon elongatus elongatus, Pseudogobio esocinus, Acheilognathus lanceolatus and Puntius tetrazona. In particular, observation of IMP distribution was conducted as an aspect of compa...
journal_title:Cell structure and function
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abstract::Autophagy is a starvation response in eukaryotes by which the cell delivers cytoplasmic components to the vacuole for degradation, and is mediated by a double membrane structure called the autophagosome. We have previously proposed that the specific combination of COPII like components, including Sec24p, is required f...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.28.49
更新日期:2003-02-01 00:00:00
abstract::Histone modifications play critical roles in the epigenetic regulation of gene expression and in the maintenance of genome integrity. Acetylation and methylation of histone H3 are particularly important in gene activation and silencing. We generated and characterized a panel of mouse monoclonal antibodies that specifi...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.07035
更新日期:2008-01-01 00:00:00
abstract::Myofibril assembly requires the cell to join diverse components, correctly oriented to the rest of the cell. Titin, a huge elastic protein with a role in myogenesis, assembles during translation in vivo and may require spatially organized mRNA to allow assembly. By immunofluorescence, we examined titin and myosin prot...
journal_title:Cell structure and function
pub_type: 杂志文章
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更新日期:1997-02-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.30.69
更新日期:2005-01-01 00:00:00
abstract::Mouse embryonic stem (mES) cells can be maintained in undifferentiated state in the presence of a cytokine, leukemia inhibitory factor (LIF). Many investigators found that STAT3 activation is important for the maintenance of pluripotency by LIF. However, the downstream pathways of STAT3 activation are still unknown. T...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.31.53
更新日期:2006-01-01 00:00:00
abstract::Two types of DNA ligase, I and II, have been purified approximately 4,000-fold from mouse testes and 500-fold from nuclei of mouse spermatocytes. DNA ligase I and II consisted of single polypeptides with molecular weights of 95,000 and 65,000, respectively, according to the estimation by SDS-polyacrylamide gel electro...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.15.67
更新日期:1990-02-01 00:00:00
abstract::Analysis of the amino acid sequence in protein (deviation analysis) suggests that the binding between trypsin and the enveloped virus is the first step of their interaction, which occurs in a specified configuration. It is possible that the distance between their active sites is important for the viral sensitivity to ...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.14.721
更新日期:1989-12-01 00:00:00
abstract::Epithelial tubules, consisting of the epithelial cell sheet with a central lumen, are the basic structure of many organs. Mechanical forces play an important role in epithelial tubulogenesis; however, little is known about the mechanisms controlling the mechanical forces during epithelial tubule morphogenesis. Solo (a...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.18010
更新日期:2018-06-02 00:00:00
abstract::The ETS family of transcription factors is defined by a conserved DNA-binding ETS domain that forms a winged helix-turn-helix structural motif. This family of transcription factors is involved in a diverse array of biological functions including cellular growth and differentiation, as well as organ development. Among ...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
doi:10.1247/csf.26.19
更新日期:2001-02-01 00:00:00
abstract::Skeletal muscle contraction is regulated by a complex of five polypeptides which are stably associated with the actin filament. This complex consists of two proteins: troponin with three subunits (TnC; TnI and TnT) and tropomyosin (a dimer of two chains). Using deletion mutants of TnC, TnI and TnT we determined that e...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
doi:10.1247/csf.22.219
更新日期:1997-02-01 00:00:00
abstract::A large number of Rab/Ypt-family small GTPases have been identified from higher plants. While some of them can complement yeast ypt mutants, the expression of Arabidopsis Ara4 protein aggravated the growth defect of a subset of ypt mutants, probably because of the titration of common regulator(s) of yeast Ypt proteins...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.25.11
更新日期:2000-02-01 00:00:00
abstract::tsJT60 is a nonlethal temperature-sensitive (ts) mutant of a Fischer rat cell line (3Y1) classified as a G0 mutant; i.e., the ts defect is not expressed within the cell growth cycle but is expressed only between the G0 and S phase. tsJT60 clones transformed with oncogenes such as adenovirus E1A, polyoma large T, polyo...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.15.385
更新日期:1990-12-01 00:00:00
abstract::Short-term and long-term (greater than 7 months) cultured astrocytes from 14-day-old rat brain were analyzed for ganglioside content. Analysis of the extracted gangliosides by HPTLC revealed that ganglioside GM1 was absent in 35 days and 235 days cultured astrocytes, and that the predominant ganglioside was GM3, showi...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.14.561
更新日期:1989-10-01 00:00:00
abstract::The efficacy of anticancer agents significantly depends on the differential susceptibility of undifferentiated cancer cells and differentiated normal cells to undergo apoptosis. We previously found that enhanced expression of RPS3a/nbl, which apparently encodes a ribosomal protein, seems to prime cells for apoptosis, ...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.25.103
更新日期:2000-04-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.19028
更新日期:2019-12-26 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.23.119
更新日期:1998-06-01 00:00:00
abstract::A protein phosphatase inhibitor, tautomycin induces blebs on the surface of human myeloid leukemia K562 cells within 10 min. In this paper we examined the cytoskeleton of tautomycin-treated cells. In the presence of tautomycin, cells with blebs turned into segmented forms with smooth surfaces after 15 min and into smo...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.18.33
更新日期:1993-02-01 00:00:00
abstract::In yeast Saccharomyces cerevisiae cells, some aberrant multimembrane-spanning proteins are not transported to the cell surface but form and are accumulated in endoplasmic reticulum (ER)-derived subcompartments, known as the ER-associated compartments (ERACs), which are observed as puncta under fluorescence microscopy....
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.19023
更新日期:2019-11-23 00:00:00
abstract::Staurosporine has been reported to cause arrest of cells in G1 phase at low concentration and in G2 phase at high concentration. This raises the question of why the effects of staurosporine on the cell cycle depend on the applied concentration. In order to verify these multiple functions of staurosporine in Meth-A cel...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.24.457
更新日期:1999-12-01 00:00:00
abstract::Significant progress has been made toward our understanding of the basic principle of nucleocytoplasmic transport, and the structure of transport factors, as well as the diversity of nucleocytoplasmic transport pathways. This review outlines the current knowledge of transport, and discusses the problems that remain as...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
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更新日期:2000-08-01 00:00:00
abstract::The purpose of the present study is to analyze the effect of serum or chick embryo extract (CEE) on the neuronal differentiation of the mouse neural crest cells. When the crest cells were cultured in the medium containing serum at low concentration (5% calf serum), neurite outgrowth was observed. The active outgrowth ...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.13.267
更新日期:1988-06-01 00:00:00
abstract::An immunocytochemical method using a specific monoclonal antibody was employed to detect DNA polymerase alpha in Drosophila melanogaster embryos during the first 13 nuclear division cycles after fertilization. The anti-DNA polymerase alpha antibody stained the ooplasm of the unfertilized egg, indicating that DNA polym...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.17.105
更新日期:1992-04-01 00:00:00
abstract::A strain of Saccharomyces cerevisiae that contains an integrated copy of a Xenopus cyclin A1 gene under the control of the GAL1 promoter has been constructed. On inducing expression of cyclin A1, the nuclear migration that occurs prior to division becomes aberrant. Instead of migrating to the neck between the mother c...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.22.465
更新日期:1997-08-01 00:00:00