Both low and high concentrations of staurosporine induce G1 arrest through down-regulation of cyclin E and cdk2 expression.

abstract：:Mouse FM3A cells propagated well in serum-free medium containing 0.5% serum albumin and 1 microgram of insulin/ml. The vitamin B12 (B12) requirement of the cells depended on the population density. This requirement disappeared when a sufficiently large cell population was present. A combination of 1-100 ng of B12/ml a...

journal_title：Cell structure and function

authors： Matsuya Y,Yamane I

更新日期：1986-03-01 00:00:00

abstract：:Significant progress has been made toward our understanding of the basic principle of nucleocytoplasmic transport, and the structure of transport factors, as well as the diversity of nucleocytoplasmic transport pathways. This review outlines the current knowledge of transport, and discusses the problems that remain as...

journal_title：Cell structure and function

authors： Imamoto N

更新日期：2000-08-01 00:00:00

abstract：:Cultured hepatic stellate cells (HSCs) are known to change their morphology and function with respect to the production of extracellular matrices (ECMs) and matrix metalloproteinases (MMPs) in response to ECM components. We examined the regulatory role of the native form of type I collagen fibrils in pro-MMP-2 product...

journal_title：Cell structure and function

authors： Wang DR,Sato M,Li LN,Miura M,Kojima N,Senoo H

更新日期：2003-12-01 00:00:00

abstract：:Tenascin-C is an oligomeric glycoprotein of the extracellular matrix that has been found to have both adhesive and anti-adhesive properties for cells. Recent elucidation of the two major TNC splice variants (320 kDa and 220 kDa) has shed light on the possibility of varying functions of the molecule based on its splici...

journal_title：Cell structure and function

authors： Ghert MA,Qi WN,Erickson HP,Block JA,Scully SP

更新日期：2001-06-01 00:00:00

abstract：:A protein phosphatase inhibitor, tautomycin induces blebs on the surface of human myeloid leukemia K562 cells within 10 min. In this paper we examined the cytoskeleton of tautomycin-treated cells. In the presence of tautomycin, cells with blebs turned into segmented forms with smooth surfaces after 15 min and into smo...

journal_title：Cell structure and function

authors： Kurisaki T,Magae J,Nagai K,Hirata A,Kusaka I,Shinji H,Morikawa M,Yoshida T,Isono K,Yamasaki M

更新日期：1993-02-01 00:00:00

abstract：:The involvement of actin filaments from the host cell on the process of invasion of trypomastigote forms of Trypanosma cruzi was analyzed in seven different cell lines. Prior incubation of all cell lines with cytochalasin D, under conditions which interfere with actin filaments, markedly inhibited parasite internaliza...

journal_title：Cell structure and function

authors： Rosestolato CT,Dutra Jda M,De Souza W,de Carvalho TM

更新日期：2002-04-01 00:00:00

abstract：:The localization of mRNA in neuronal dendrites plays a role in both locally and temporally regulated protein synthesis, which is required for certain forms of synaptic plasticity. RNA granules constitute a dendritic mRNA transport machinery in neurons, which move along microtubules. RNA granules contain densely packed...

journal_title：Cell structure and function

authors： Miki T,Takano K,Yoneda Y

更新日期：2005-01-01 00:00:00

abstract：:We earlier discovered a novel 40-kDa protein (hsp40) induced by heat shock and other stresses in mammalian and avian cells. In this report, we purified the hsp40 in HeLa cells, using modified two-dimensional gel electrophoresis, and determined the amino terminal amino acid sequence of this protein. The hsp40 is homolo...

journal_title：Cell structure and function

authors： Hattori H,Liu YC,Tohnai I,Ueda M,Kaneda T,Kobayashi T,Tanabe K,Ohtsuka K

更新日期：1992-02-01 00:00:00

abstract：:Numerous types of cancer cells migrate into extracellular tissues. This phenomenon is termed invasion, and is associated with poor prognosis in cancer patients. In this study, we demonstrated that filamin B (FLNb), an actin-binding protein, is highly expressed in cancer cell lines that exhibit high invasiveness, with ...

journal_title：Cell structure and function

authors： Iguchi Y,Ishihara S,Uchida Y,Tajima K,Mizutani T,Kawabata K,Haga H

更新日期：2015-01-01 00:00:00

abstract：:We constructed a fusion protein of the cell-binding domain of human fibronectin and human basic fibroblast growth factor, and prepared a polypeptide with both cell-adhesive activity and growth factor activity. A human gene fragment coding for basic fibroblast growth factor was amplified by the polymerase chain reactio...

journal_title：Cell structure and function

authors： Hashi H,Hatai M,Kimizuka F,Kato I,Yaoi Y

更新日期：1994-02-01 00:00:00

abstract：:It has been shown that in PC12 and its subclone PC12h treatment of the cells with nerve growth factor (NGF) induces a selective decrease in the incorporation of radioactive phosphate into a 100,000-dalton protein, designated in an earlier study as Nsp100, in the subsequent phosphorylation of soluble extracts from cell...

journal_title：Cell structure and function

authors： Hashimoto S,Hagino A

更新日期：1989-02-01 00:00:00

abstract：:A monoclonal type M-immunoglobulin (IgM) was generated in mice against a nuclease-urea extract of HeLa metaphase chromosomes. This antibody stains metaphase chromosomes from a variety of mammalian cultured cell types by indirect immunofluorescence. Antibody 12C7 reacts by western transfer technique with histone H1 in ...

journal_title：Cell structure and function

authors： Ortiz M,Bejarano LA,Rendón MC,Martin G,Brinkley BR,Valdivia MM

更新日期：1991-08-01 00:00:00

abstract：:Hepatic stellate cells (HSCs) were changed in their morphology, proliferative activity, and functions by culturing on type I collagen gel, as compared to the culture on polystyrene surface. HSCs have been found to produce extracellular matrix components and matrix metalloproteinases (MMPs). In this study, we have asse...

journal_title：Cell structure and function

authors： Li YL,Sato M,Kojima N,Miura M,Senoo H

更新日期：1999-10-01 00:00:00

abstract：:Cholera toxin binding sites in frozen sections of rat thymus were studied by indirect immunofluorescence and were found to be localized selectively in the medulla of thymic lobules. By flow cytometric analysis, two populations of thymocytes differing in size and reactivity towards cholera toxin were detected; the smal...

journal_title：Cell structure and function

authors： Bouchon B,Iwamori M,Masago H,Nagai Y

更新日期：1987-08-01 00:00:00

abstract：:The distribution of Ca2+ in dividing cells of the maize root tip was examined by potassium pyroantimonate precipitation and EGTA treatment methods. Ca2+ was found in most of the cell organelles, such as the matrix of the mitochondria, the thylakoid membrane of the proplastid and the Golgi vesicles, and on the plasma m...

journal_title：Cell structure and function

authors： Sakai-Wada A,Yagi S

更新日期：1993-12-01 00:00:00

abstract：:When spores of Dictyostelium discoideum matured through normal development were stained with rhodamine-phalloidin, rod-shaped fluorescence was found across the nucleus and in the cytoplasm. Electron micrographs of the rod shape showed that it consisted of long and straight fibers 12 nm in diameter. In contrast, a conv...

journal_title：Cell structure and function

authors： Sameshima M,Chijiiwa Y,Kishi Y,Hashimoto Y

更新日期：1994-08-01 00:00:00

abstract：:tsJT60 is a nonlethal temperature-sensitive (ts) mutant of a Fischer rat cell line (3Y1) classified as a G0 mutant; i.e., the ts defect is not expressed within the cell growth cycle but is expressed only between the G0 and S phase. tsJT60 clones transformed with oncogenes such as adenovirus E1A, polyoma large T, polyo...

journal_title：Cell structure and function

authors： Tai YY,Ninomiya-Tsuji J,Furuoku K,Ogawa N,Ishibashi S,Shiroki K,Segawa K,Tsuchida N,Shibuya M,Ide T

更新日期：1990-12-01 00:00:00

abstract：:alpha-1,3-Fucosyltransferase (Fuc T IV) cDNA was placed under the control of beta-actin, cytomegalovirus enhancer/promoter and transfected into embryonic stem cells. The transfected cell clones with integrated cDNA (positive clones) differentiated more efficiently into myocardial cell clones without integrated cDNA (n...

journal_title：Cell structure and function

authors： Sudou A,Muramatsu H,Kaname T,Kadomatsu K,Muramatsu T

更新日期：1997-04-01 00:00:00

abstract：:A granulocyte colony-stimulating factor (G-CSF) was highly purified from the serum-free culture medium of RSP -2 X P3 cells. The G-CSF had an apparent molecular weight of 33,000 as determined by high speed gel permeation chromatography, but its molecular weight was decreased to 15,000 by 0.1% sodium dodecyl sulfate. A...

journal_title：Cell structure and function

authors： Tsuneoka K,Shikita M

更新日期：1984-03-01 00:00:00

abstract：:The Saccharomyces cerevisiae autophagy-initiation complex, Atg1 kinase complex, consists of Atg1, Atg13, Atg17, Atg29, and Atg31, while the corresponding complex in most other eukaryotes, including mammals, is composed of ULK1 (or ULK2), Atg13, FIP200 (also known as RB1CC1), and Atg101. ULKs are homologs of Atg1, and ...

journal_title：Cell structure and function

authors： Noda NN,Mizushima N

更新日期：2016-01-01 00:00:00

abstract：:Embryonic stem (ES) cells are a pluripotent cell line derived from the inner cell mass of the mouse blastocyst. The mRNA levels of several growth-related genes were examined in ES cells by Northern blot analysis under several different growth conditions. In a coculture of ES cells with the mouse fibroblast cell line, ...

journal_title：Cell structure and function

authors： Katsube K,Shimizu N

更新日期：1991-10-01 00:00:00

abstract：:Myofibril assembly requires the cell to join diverse components, correctly oriented to the rest of the cell. Titin, a huge elastic protein with a role in myogenesis, assembles during translation in vivo and may require spatially organized mRNA to allow assembly. By immunofluorescence, we examined titin and myosin prot...

journal_title：Cell structure and function

authors： Fulton AB,Alftine C

更新日期：1997-02-01 00:00:00

abstract：:The substance in sperm which induces the activation of eggs at fertilization has yet to be elucidated. Osawa et al. reported that the soluble extract of sperm (spex) causes inositol 1,4,5-trisphosphate (IP3)-induced Ca2+ release (IICR) in fertilized eggs of sea urchins when externally applied [Osawa et al., (1992). Zo...

journal_title：Cell structure and function

authors： Osawa M

更新日期：1994-04-01 00:00:00

abstract：:The morphology and intramembranous particle (IMP) distribution of spermatozoa were investigated in four species of cyprinid fishes, Gnathopogon elongatus elongatus, Pseudogobio esocinus, Acheilognathus lanceolatus and Puntius tetrazona. In particular, observation of IMP distribution was conducted as an aspect of compa...

journal_title：Cell structure and function

authors： Ohta T,Matsuda M

更新日期：1995-08-01 00:00:00

abstract：:Synapsin I, one of the major synaptic proteins, is thought to associate with synaptic vesicles and to play a regulatory role in neurotransmitter release. In mature neurons, it is concentrated almost exclusively in presynaptic nerve endings. Here, we studied the subcellular localization of synapsin I during the develop...

journal_title：Cell structure and function

authors： Harada A,Sobue K,Hirokawa N

更新日期：1990-12-01 00:00:00

abstract：:It has been suggested that the organization of microtubules during mitosis plays an important role in cytokinesis in animal cells. We studied the organization of microtubules during the first cleavage and its role in cytokinesis of Xenopus eggs. First, we examined the immunofluorescent localization of microtubules in ...

journal_title：Cell structure and function

authors： Takayama M,Noguchi T,Yamashiro S,Mabuchi I

更新日期：2002-08-01 00:00:00

abstract：:We have found that 1,9-Dideoxyforskolin (DDF) strongly inhibited the cell death induced by ricin, modeccin, Pseudomonas toxin, and diphtheria toxin in MDCK cells, suggesting that these protein toxins have a DDF-sensitive common pathway leading to cell death. However, no significant effect of forskolin on these toxins ...

journal_title：Cell structure and function

authors： Oda T,Komatsu N,Muramatsu T

更新日期：1997-10-01 00:00:00

abstract：:Contact reaction is the morphological expression of allogeneic recognition by hemocytes in the ascidian, Halocynthia roretzi; namely they undergo an exocytotic burst upon contact with non-self (allogeneic) hemocytes in vitro. We have found that contact reaction is accompanied by a burst of cyanide-insensitive oxygen c...

journal_title：Cell structure and function

authors： Akita N,Hoshi M

更新日期：1995-02-01 00:00:00

abstract：:DNA-dependent protein kinase (DNA-PK), consisting of the 470-kDa catalytic component (DNA-PKcs) and the DNA-binding regulatory component Ku protein (p70/p80), catalyzes phosphorylation of a variety of DNA replication/transcription/repair factors in the presence of double-stranded DNA. In the resting states of human pe...

journal_title：Cell structure and function

authors： Nagasawa M,Watanabe F,Suwa A,Yamamoto K,Tsukada K,Teraoka H

更新日期：1997-12-01 00:00:00

abstract：:To elucidate the cytotoxic mechanism of tumor necrosis factor (TNF), we isolated TNF-resistant sublines of L929 cells. As compared with L929 cells, TNF-resistant cells retained similar number and affinity of TNF-binding sites, and showed a similar growth rate. TNF stimulated arachidonate release from L929 cells, while...

journal_title：Cell structure and function

authors： Hayakawa M,Oku N,Takagi T,Hori T,Shibamoto S,Yamanaka Y,Takeuchi K,Tsujimoto M,Ito F

更新日期：1991-08-01 00:00:00