Novel actin rods appeared in spores of Dictyostelium discoideum.

abstract：:The Golgi stress response is a homeostatic mechanism that controls the capacity of the Golgi apparatus in accordance with cellular demands. When the capacity of the Golgi apparatus becomes insufficient (Golgi stress), transcription levels of Golgi-related genes encoding glycosylation enzymes, a Golgi structural protei...

journal_title：Cell structure and function

authors： Taniguchi M,Sasaki-Osugi K,Oku M,Sawaguchi S,Tanakura S,Kawai Y,Wakabayashi S,Yoshida H

更新日期：2016-07-30 00:00:00

abstract：:1. Cofilin is an essential actin-regulating protein widely distributed in all eucaryotes. The structure and function of cofilin are conserved during evolution. 2. Cofilin depolymerizes F-actin in vitro at alkaline pH and severs F-actin in vitro at pH lower than 7.3. Overexpression of cofilin in viable cells induced bu...

journal_title：Cell structure and function

authors： Yahara I,Aizawa H,Moriyama K,Iida K,Yonezawa N,Nishida E,Hatanaka H,Inagaki F

更新日期：1996-10-01 00:00:00

abstract：:CD9 and CD63 belong to a tetramembrane-spanning glycoprotein family called tetraspanin, and are involved in a wide variety of cellular processes, but the structure-function relationship of this family of proteins has yet to be clarified. CD9 associates with diphtheria toxin receptor (DTR), which is identical to the me...

journal_title：Cell structure and function

authors： Ryu F,Takahashi T,Nakamura K,Takahashi Y,Kobayashi T,Shida S,Kameyama T,Mekada E

更新日期：2000-10-01 00:00:00

abstract：:Vascular endothelial cells possess antithrombotic properties, which are determined by the balance between plasminogen activators (PAs) and PA inhibitors (PAls). A cell line, TKM-33, has been established and cloned from human umbilical vein endothelial cells, was previously reported to produce a large amount of urokina...

journal_title：Cell structure and function

authors： Ueshima S,Matsumoto H,Izaki S,Mitsui Y,Fukao H,Okada K,Matsuo O

更新日期：1999-04-01 00:00:00

abstract：:Caffeine has been shown to enhance the lethal effect of DNA-damaging agents in mammalian cells, and the potentiation by caffeine of this effect is generally interpreted as the result of inhibition by caffeine of the repair of damaged DNA. However, the mechanism by which caffeine enhances the lethal effect of DNA-damag...

journal_title：Cell structure and function

authors： Ishida R,Kozaki M,Takahashi T

更新日期：1985-12-01 00:00:00

abstract：:The discovery of fluorescent proteins (FPs) has revolutionized cell biology. The fusion of targeting sequences to FPs enables the investigation of cellular organelles and their dynamics; however, occasionally, such fluorescent fusion proteins (FFPs) exhibit behavior different from that of the native proteins. Here, we...

journal_title：Cell structure and function

authors： Kashiwagi S,Fujioka Y,Satoh AO,Yoshida A,Fujioka M,Nepal P,Tsuzuki A,Aoki O,Paudel S,Sasajima H,Ohba Y

更新日期：2019-12-26 00:00:00

abstract：:NBT-II cells on collagen-coated substrates move rapidly and persistently, maintaining a semi-circular shape with a large lamellipodium, in a manner similar to fish keratocytes. The inhibitor of phospholipase D (PLD), n-butanol, completely blocked the migration and disturbed the characteristic localization of actin alo...

journal_title：Cell structure and function

authors： Nagasaki A,Inotsume K,Kanada M,Uyeda TQ

更新日期：2008-01-01 00:00:00

abstract：:Here, we would like to point out important milestones in the study of nuclear radial positioning and gene expression during differentiation processes. In addition, changes in the histone signature that significantly precede various differentiation pathways are reviewed. We address the regulatory functions of chromatin...

journal_title：Cell structure and function

authors： Harnicarová Horáková A,Bártová E,Kozubek S

更新日期：2010-01-01 00:00:00

abstract：:The efficacy of anticancer agents significantly depends on the differential susceptibility of undifferentiated cancer cells and differentiated normal cells to undergo apoptosis. We previously found that enhanced expression of RPS3a/nbl, which apparently encodes a ribosomal protein, seems to prime cells for apoptosis, ...

journal_title：Cell structure and function

authors： Russell L,Naora H,Naora H

更新日期：2000-04-01 00:00:00

abstract：:Genomics and proteomics studies in Chlamydomonas have revealed that an axoneme is composed of 200-600 types of proteins, including uncharacterized proteins collectively named flagellar-associated proteins (FAPs). Nine FAPs contain the EF-hand motif; however, they have not yet been well characterized. To find component...

journal_title：Cell structure and function

authors： Kirima J,Oiwa K

更新日期：2018-02-16 00:00:00

abstract：:Recent studies have revealed various biological functions for repetitive sequences, which make up about half of the human genome. One such sequence, major satellites, which are tandem repetitive sequences adjacent to the centromere, have been shown to be a kinetochore component that plays a role in the formation and f...

journal_title：Cell structure and function

authors： Kishi Y,Kondo S,Gotoh Y

更新日期：2012-01-01 00:00:00

abstract：:Spindle pole bodies (SPBs) attached to nuclei were isolated from Saccharomyces cerevisiae; they nucleated microtubules to form asters in vitro. This aster-forming activity was stable in 30% dimethylsulfoxide or 10% 2-propanol and could be solubilized with KCl. The KCl extract contained many protein components, which a...

journal_title：Cell structure and function

authors： Yamamoto A,Nagai K,Yamasaki M,Matsuhashi M

更新日期：1990-08-01 00:00:00

abstract：:Marvelous background rejection in total internal reflection fluorescence microscopy (TIR-FM) has made it possible to visualize single-fluorophores in living cells. Cell signaling proteins including peptide hormones, membrane receptors, small G proteins, cytoplasmic kinases as well as small signaling compounds have bee...

journal_title：Cell structure and function

authors： Sako Y,Uyemura T

更新日期：2002-10-01 00:00:00

abstract：:The role of microtubule associated proteins (MAPs) on the dynamic instability of microtubules was examined under a dark-field microscope using bovine brain tubulin purified by DEAE-Sepharose column chromatography. In the absence of MAPs, the transition from the shortening phase to the growing phase (the rescue) occurr...

journal_title：Cell structure and function

authors： Itoh TJ,Hotani H

更新日期：1994-10-01 00:00:00

abstract：:Distribution of peanut agglutinin binding sites was studied histologically with horseradish peroxidase labelled and fluorescein isothiocyanate labelled peanut agglutinin in terms of cell differentiation in rat lymphatic organs, (thymus, spleen, lymph nodes). In this study, alcohol-fixed paraffin-embedded tissue sectio...

journal_title：Cell structure and function

authors： Kabir A,Watanabe M,Takeda Z,Kizaki T,Urano Y

更新日期：1983-03-01 00:00:00

abstract：:Two types of DNA ligase, I and II, have been purified approximately 4,000-fold from mouse testes and 500-fold from nuclei of mouse spermatocytes. DNA ligase I and II consisted of single polypeptides with molecular weights of 95,000 and 65,000, respectively, according to the estimation by SDS-polyacrylamide gel electro...

journal_title：Cell structure and function

authors： Higashitani A,Tabata S,Endo H,Hotta Y

更新日期：1990-02-01 00:00:00

abstract：:The precise control of spindle microtubule (MT) dynamics is essential for chromosome capture and alignment. Kif18A/kinesin-8, an essential regulator of kinetochore MT dynamics, accumulates at its plus-ends in metaphase but not prometaphase cells. The underlying mechanism of time-dependent and kinetochore MT-specific p...

journal_title：Cell structure and function

authors： Masuda N,Shimodaira T,Shiu SJ,Tokai-Nishizumi N,Yamamoto T,Ohsugi M

更新日期：2011-01-01 00:00:00

abstract：:Effects of kinase inhibitors on the preprophase band of microtubules in onion (Allium cepa L.) root tip cells were examined. Bundled microtubules in preprophase bands were dispersed on the cell cortex when onion seedlings were incubated with 2.5-5.0 mM 6-dimethylaminopurine. Fifteen min was enough for the bundled micr...

journal_title：Cell structure and function

authors： Nogami A,Mineyuki Y

更新日期：1999-10-01 00:00:00

abstract：:The distribution of Ca2+ in dividing cells of the maize root tip was examined by potassium pyroantimonate precipitation and EGTA treatment methods. Ca2+ was found in most of the cell organelles, such as the matrix of the mitochondria, the thylakoid membrane of the proplastid and the Golgi vesicles, and on the plasma m...

journal_title：Cell structure and function

authors： Sakai-Wada A,Yagi S

更新日期：1993-12-01 00:00:00

abstract：:The purpose of the present study is to analyze the effect of serum or chick embryo extract (CEE) on the neuronal differentiation of the mouse neural crest cells. When the crest cells were cultured in the medium containing serum at low concentration (5% calf serum), neurite outgrowth was observed. The active outgrowth ...

journal_title：Cell structure and function

authors： Ito K,Morita T,Takeuchi T

更新日期：1988-06-01 00:00:00

abstract：:Hepatic stellate cells (HSCs), a mesenchymal cell type in hepatic parenchyma, have unique features with respect to their cellular origin, morphology, and function. Normal, quiescent HSCs function as major vitamin A-storing cells containing over 80% of total vitamin A in the body to maintain vitamin A homeostasis. HSCs...

journal_title：Cell structure and function

authors： Sato M,Suzuki S,Senoo H

更新日期：2003-04-01 00:00:00

abstract：:Embryonic stem (ES) cells are a pluripotent cell line derived from the inner cell mass of the mouse blastocyst. The mRNA levels of several growth-related genes were examined in ES cells by Northern blot analysis under several different growth conditions. In a coculture of ES cells with the mouse fibroblast cell line, ...

journal_title：Cell structure and function

authors： Katsube K,Shimizu N

更新日期：1991-10-01 00:00:00

abstract：:It has been shown that in PC12 and its subclone PC12h treatment of the cells with nerve growth factor (NGF) induces a selective decrease in the incorporation of radioactive phosphate into a 100,000-dalton protein, designated in an earlier study as Nsp100, in the subsequent phosphorylation of soluble extracts from cell...

journal_title：Cell structure and function

authors： Hashimoto S,Hagino A

更新日期：1989-02-01 00:00:00

abstract：:The relative fibrin-binding, fibrinolytic and fibrinogenolytic properties of single-chain pro-urokinase, an inactive proenzyme form of human urokinase purified from cultured human kidney cells, and urokinase were compared. The affinity of single-chain pro-urokinase for fibrin was much higher than that of urokinase. In...

journal_title：Cell structure and function

authors： Kasai S,Arimura H,Nishida M,Suyama T

更新日期：1985-06-01 00:00:00

abstract：:The in vivo structure of the smooth endoplasmic reticulum (ER) was visualized in rat and mouse cerebellar Purkinje cells by using quick-freezing techniques followed by freeze-substitution for ultrathin-sectioning or freeze-fracturing and deep-etching for replicas. High magnification electron microscopy of the ultrathi...

journal_title：Cell structure and function

authors： Kanaseki T,Ikeuchi Y,Tashiro Y

更新日期：1998-12-01 00:00:00

abstract：:One of the proposed roles of sarcoglycan is to stabilize dystrophin glycoprotein complexes in muscle sarcolemma. Involvement in signal transduction has also been proposed and abnormalities in some sarcoglycan genes are known to be responsible for muscular dystrophy. While characterization of sarcoglycans in muscle has...

journal_title：Cell structure and function

authors： Hashimoto R,Yamaguchi M

更新日期：2006-01-01 00:00:00

abstract：:Previously, we serially transplanted tumors that produced colony-stimulating factor (CSF) into nude mice, who developed marked granulocytosis along with tumor growth; their leukocyte counts reaching approximately one million per cu mm. The numbers of CFU-GM, CFU-E, CFU-Meg, CFU-S and BFU-E were increased in nude mice ...

journal_title：Cell structure and function

authors： Takeda K,Suda T,Sudo T,Miura Y

更新日期：1987-06-01 00:00:00

abstract：:The unique Physarum lysophosphatidic acid, PHYLPA, having a cyclopropane in the fatty acid moiety and a cyclic phosphate at C-2 and C-3 positions of the glycerol, inhibited proliferation of human fibroblast cells, TIG-3 and TIG-7, which were cultured in a chemically defined (serum-free) medium. The cells at S- and M-p...

journal_title：Cell structure and function

authors： Murakami-Murofushi K,Kaji K,Kano K,Fukuda M,Shioda M,Murofushi H

更新日期：1993-10-01 00:00:00

abstract：:A granulocyte colony-stimulating factor (G-CSF) was highly purified from the serum-free culture medium of RSP -2 X P3 cells. The G-CSF had an apparent molecular weight of 33,000 as determined by high speed gel permeation chromatography, but its molecular weight was decreased to 15,000 by 0.1% sodium dodecyl sulfate. A...

journal_title：Cell structure and function

authors： Tsuneoka K,Shikita M

更新日期：1984-03-01 00:00:00

abstract：:The Saccharomyces cerevisiae autophagy-initiation complex, Atg1 kinase complex, consists of Atg1, Atg13, Atg17, Atg29, and Atg31, while the corresponding complex in most other eukaryotes, including mammals, is composed of ULK1 (or ULK2), Atg13, FIP200 (also known as RB1CC1), and Atg101. ULKs are homologs of Atg1, and ...

journal_title：Cell structure and function

authors： Noda NN,Mizushima N

更新日期：2016-01-01 00:00:00