Abstract:
:Caffeine has been shown to enhance the lethal effect of DNA-damaging agents in mammalian cells, and the potentiation by caffeine of this effect is generally interpreted as the result of inhibition by caffeine of the repair of damaged DNA. However, the mechanism by which caffeine enhances the lethal effect of DNA-damaging agents has not yet been elucidated. During studies on the effect of caffeine on DNA repair, we found by alkaline elution analysis that caffeine alone produced DNA strand breaks or alkali labile sites in Chinese hamster ovary cells. The amount of DNA breakage or alkali labile sites depended on the concentration of caffeine. We propose that DNA breakage induced by caffeine may be involved in the enhancement of the lethal effect of DNA-damaging agents.
journal_name
Cell Struct Functjournal_title
Cell structure and functionauthors
Ishida R,Kozaki M,Takahashi Tdoi
10.1247/csf.10.405subject
Has Abstractpub_date
1985-12-01 00:00:00pages
405-9issue
4eissn
0386-7196issn
1347-3700journal_volume
10pub_type
杂志文章abstract::In yeast Saccharomyces cerevisiae cells, some aberrant multimembrane-spanning proteins are not transported to the cell surface but form and are accumulated in endoplasmic reticulum (ER)-derived subcompartments, known as the ER-associated compartments (ERACs), which are observed as puncta under fluorescence microscopy....
journal_title:Cell structure and function
pub_type: 杂志文章
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abstract::Many studies on in vitro transformation of human cells indicate that the cells must be immortalized before they can be neoplastically transformed, indicating that immortalization is a critical step in multistep neoplastic transformation of human cells. We immortalized three human cell lines by repeated treatment with ...
journal_title:Cell structure and function
pub_type: 杂志文章
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abstract::Cultured hepatic stellate cells (HSCs) are known to change their morphology and function with respect to the production of extracellular matrices (ECMs) and matrix metalloproteinases (MMPs) in response to ECM components. We examined the regulatory role of the native form of type I collagen fibrils in pro-MMP-2 product...
journal_title:Cell structure and function
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journal_title:Cell structure and function
pub_type: 杂志文章,评审
doi:10.1247/csf.09021
更新日期:2010-01-01 00:00:00
abstract::Tritrichomonas foetus strongly agglutinates human erythrocytes, thus suggesting the occurrence of an adhesin associated with its surface. Adherence was observed immediately after mixing of the parasites with erythrocytes and the intensity increased for up to 30 min. Scanning electron microscopy examination of T. foetu...
journal_title:Cell structure and function
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doi:10.1247/csf.21.245
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abstract::NBT-II cells on collagen-coated substrates move rapidly and persistently, maintaining a semi-circular shape with a large lamellipodium, in a manner similar to fish keratocytes. The inhibitor of phospholipase D (PLD), n-butanol, completely blocked the migration and disturbed the characteristic localization of actin alo...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.07042
更新日期:2008-01-01 00:00:00
abstract::CD9 and CD63 belong to a tetramembrane-spanning glycoprotein family called tetraspanin, and are involved in a wide variety of cellular processes, but the structure-function relationship of this family of proteins has yet to be clarified. CD9 associates with diphtheria toxin receptor (DTR), which is identical to the me...
journal_title:Cell structure and function
pub_type: 杂志文章
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.10.271
更新日期:1985-09-01 00:00:00
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journal_title:Cell structure and function
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更新日期:1984-06-01 00:00:00
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journal_title:Cell structure and function
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更新日期:1990-12-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
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更新日期:1987-10-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.15.67
更新日期:1990-02-01 00:00:00
abstract::Embryonic stem (ES) cells are a pluripotent cell line derived from the inner cell mass of the mouse blastocyst. The mRNA levels of several growth-related genes were examined in ES cells by Northern blot analysis under several different growth conditions. In a coculture of ES cells with the mouse fibroblast cell line, ...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.16.375
更新日期:1991-10-01 00:00:00
abstract::In adult rat cerebellum, HPC-1/syntaxin 1 is detected at high density on the plasma membrane of the non-synaptic region of parallel fibers in addition to the synaptic terminal membranes and the synaptic vesicles (Koh, S., Yamamoto, A., Inoue, A., Inoue, Y., Akagawa, Y., Kawamura, Y., Kawamoto, Y., and Tashiro, Y. (199...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.21.525
更新日期:1996-12-01 00:00:00
abstract::The fine structural localization of albumin in rat liver parenchymal cells was determined by an improved immunocytochemical method and serial sectioning. Albumin in the secretory apparatus of the parenchymal cells was present in segments of the rough endoplasmic reticulum, interrupted with negative segments, in transp...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.12.251
更新日期:1987-06-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.12.339
更新日期:1987-08-01 00:00:00
abstract::Skeletal muscle contraction is regulated by a complex of five polypeptides which are stably associated with the actin filament. This complex consists of two proteins: troponin with three subunits (TnC; TnI and TnT) and tropomyosin (a dimer of two chains). Using deletion mutants of TnC, TnI and TnT we determined that e...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
doi:10.1247/csf.22.219
更新日期:1997-02-01 00:00:00
abstract::Cytokeratin 8 (CK8) and cytokeratin 19 (CK19) is a specific cytoskeletal component of simple epithelia, including bronchial epithelial cells. We hypothesized that CK8 or CK19 released from epithelial cells may bind to and cause damage to extracellular matrixes through binding of anti-CK8 or anti-CK19 autoantibodies. I...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.25.171
更新日期:2000-06-01 00:00:00
abstract::The ETS family of transcription factors is defined by a conserved DNA-binding ETS domain that forms a winged helix-turn-helix structural motif. This family of transcription factors is involved in a diverse array of biological functions including cellular growth and differentiation, as well as organ development. Among ...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
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更新日期:2001-02-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.12.273
更新日期:1987-06-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.14.193
更新日期:1989-04-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.12.127
更新日期:1987-02-01 00:00:00
abstract::When spores of Dictyostelium discoideum matured through normal development were stained with rhodamine-phalloidin, rod-shaped fluorescence was found across the nucleus and in the cytoplasm. Electron micrographs of the rod shape showed that it consisted of long and straight fibers 12 nm in diameter. In contrast, a conv...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.19.189
更新日期:1994-08-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.9.67
更新日期:1984-03-01 00:00:00
abstract::We have previously shown that mouse L.P3 cells secrete fibronectin and a novel protein, gelatin-non-binding and heparin-binding cell-adhesive protein (GNCP). Here, we screened and characterized cell-adhesive proteins in the conditioned media (CM's) of a series of cell lines growing in a protein- and lipid-free synthet...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.18.61
更新日期:1993-02-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.30.69
更新日期:2005-01-01 00:00:00
abstract::Upon tryptic digestion of synaptosomes, ATPase activities decreased in the order of Cl(-)-ATPase greater than or equal to Na+,K+-ATPase greater than anion-insensitive Mg2+-ATPase. Upon synaptosome treatment with hypotonic solution, Cl(-)-ATPase or anion-insensitive Mg2+-ATPase was slightly inactivated, while Na+,K+-AT...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.13.105
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.14.721
更新日期:1989-12-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.15.329
更新日期:1990-12-01 00:00:00
abstract::Checkpoint kinase 1 (Chk1) is a conserved protein kinase central to the cell-cycle checkpoint during DNA damage response (DDR). Until recently, ATR, a protein kinase activated in response to DNA damage or stalled replication, has been considered as the sole regulator of Chk1. Recent progress, however, has led to the i...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
doi:10.1247/csf.14017
更新日期:2015-01-01 00:00:00