Abstract:
:NBT-II cells on collagen-coated substrates move rapidly and persistently, maintaining a semi-circular shape with a large lamellipodium, in a manner similar to fish keratocytes. The inhibitor of phospholipase D (PLD), n-butanol, completely blocked the migration and disturbed the characteristic localization of actin along the edge of lamellipodia. To investigate the functional difference between the two isozymes of PLD (PLD1 and PLD2), we transfected NBT-II cells with vectors expressing shRNA to deplete PLD1 or PLD2. Depletion of both PLD1 and 2 by RNA interference reduced the velocity of the migration, but depletion of PLD2 inhibited motility more severely than that of PLD1. Furthermore, GFP-PLD2 was localized to the protruding regions of lamellipodia in migrating cells. Thus, PLD is essential for the maintenance of keratocyte-like locomotion of NBT-II cells, presumably by regulating the actin cytoskeleton.
journal_name
Cell Struct Functjournal_title
Cell structure and functionauthors
Nagasaki A,Inotsume K,Kanada M,Uyeda TQdoi
10.1247/csf.07042subject
Has Abstractpub_date
2008-01-01 00:00:00pages
27-33issue
1eissn
0386-7196issn
1347-3700pii
JST.JSTAGE/csf/07042journal_volume
33pub_type
杂志文章abstract::The transcription factor ATF6 is constitutively synthesized as a type II transmembrane protein embedded in the endoplasmic reticulum (ER). When unfolded proteins accumulate in the ER, ATF6 senses such ER stress via an as yet undetermined mechanism and relocates to the Golgi apparatus where it is cleaved by sequential ...
journal_title:Cell structure and function
pub_type: 杂志文章
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journal_title:Cell structure and function
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journal_title:Cell structure and function
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更新日期:2018-02-16 00:00:00
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journal_title:Cell structure and function
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pub_type: 杂志文章
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.22.545
更新日期:1997-10-01 00:00:00
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pub_type: 杂志文章,评审
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journal_title:Cell structure and function
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journal_title:Cell structure and function
pub_type: 杂志文章
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journal_title:Cell structure and function
pub_type: 杂志文章
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journal_title:Cell structure and function
pub_type: 杂志文章
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journal_title:Cell structure and function
pub_type: 杂志文章
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.18.73
更新日期:1993-02-01 00:00:00
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更新日期:1999-04-01 00:00:00