Further characterization of the cation channel of a yeast vacuolar membrane in a planar lipid bilayer.

Abstract:

:A voltage-dependent and Ca2(+)-activated cation channel recently found in the vacuolar membrane of the yeast Saccharomyces cerevisiae was incorporated into planar lipid bilayers and further characterized in macroscopic and single channel levels. Single channel conductances for various cations were in the order: NH4+ greater than K+ greater than Rb+ greater than Cs+ greater than Na+ greater than Li+, and were nearly consistent with the order of permeability ratio estimated from reversal potentials determined by macroscopic measurement. Up to 6 mM of Ca2+ added to the cis (cytoplasmic) side opened the channel, but higher concentrations closed the channel without affecting the single channel conductance. Ba2+ closed the channel without affecting the single channel conductance. Ba2+ closed the channel from the cis side. In addition to the above channel, a small cation-selective channel of about 40 pS was found.

journal_name

Cell Struct Funct

authors

Sato M,Tanifuji M,Kasai M

doi

10.1247/csf.14.659

subject

Has Abstract

pub_date

1989-12-01 00:00:00

pages

659-68

issue

6

eissn

0386-7196

issn

1347-3700

journal_volume

14

pub_type

杂志文章
  • Pericentric H3K9me3 Formation by HP1 Interaction-defective Histone Methyltransferase Suv39h1.

    abstract::Pericentric regions form epigenetically organized, silent heterochromatin structures that accumulate histone H3 lysine 9 tri-methylation (H3K9me3) and heterochromatin protein 1 (HP1), a methylated H3K9-binding protein. At pericentric regions, Suv39h is the major enzyme that generates H3K9me3. Suv39h also interacts dir...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.16013

    authors: Muramatsu D,Kimura H,Kotoshiba K,Tachibana M,Shinkai Y

    更新日期:2016-12-03 00:00:00

  • Role of VAMP8/endobrevin in constitutive exocytotic pathway in HeLa cells.

    abstract::To evaluate the role of VAMP8/endobrevin in constitutive exocytosis, we have examined the exocytotic pathways of VAMP8 and human growth hormone, both GFP-tagged, by total internal reflection fluorescence microscopy (TIRF-M). Human GH-GFP and VAMP8-GFP were similarly expressed in small round vesicles and elongated tubu...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.09013

    authors: Okayama M,Arakawa T,Tanimura A,Mizoguchi I,Tajima Y,Takuma T

    更新日期:2009-01-01 00:00:00

  • Rough surfaced smooth endoplasmic reticulum in rat and mouse cerebellar Purkinje cells visualized by quick-freezing techniques.

    abstract::The in vivo structure of the smooth endoplasmic reticulum (ER) was visualized in rat and mouse cerebellar Purkinje cells by using quick-freezing techniques followed by freeze-substitution for ultrathin-sectioning or freeze-fracturing and deep-etching for replicas. High magnification electron microscopy of the ultrathi...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.23.373

    authors: Kanaseki T,Ikeuchi Y,Tashiro Y

    更新日期:1998-12-01 00:00:00

  • The effect of cytokines on the differentiation of an eosinophilic leukemia cell line (EoL-1) is associated with down regulation of c-myc gene expression.

    abstract::In this study, we measured hydrogen peroxide (H2O2) release as one of the functions of mature eosinophils, and utilized it as a quantitative index. We demonstrated that 1) the human eosinophilic leukemia cell line, EoL-1, did not release H2O2 when stimulated with phorbol myristate acetate (PMA), but after culturing wi...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.18.125

    authors: Ohtsu H,Yamauchi K,Yoshie O,Tanno Y,Saito H,Hayashi N,Takishima T

    更新日期:1993-04-01 00:00:00

  • Transmembrane delivery of polypeptide growth factors bypassing the intrinsic cell surface receptors: synthesis and biological activity of a conjugate of epidermal growth factor with alpha 2-macroglobulin.

    abstract::Epidermal growth factor (EGF) was derivatized at the amino terminus with N-succinimidyl 3-(2-pyridyldithio)propionate and then cross-linked to the cysteinyl residues of alpha 2-macroglobulin (alpha 2M) via disulfide bonds. The EGF-alpha 2M conjugate delivered EGF into dense lysosomal fractions through binding to alpha...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.9.105

    authors: Ito F,Ito S,Shimizu N

    更新日期:1984-06-01 00:00:00

  • A role of cofilin/destrin in reorganization of actin cytoskeleton in response to stresses and cell stimuli.

    abstract::1. Cofilin is an essential actin-regulating protein widely distributed in all eucaryotes. The structure and function of cofilin are conserved during evolution. 2. Cofilin depolymerizes F-actin in vitro at alkaline pH and severs F-actin in vitro at pH lower than 7.3. Overexpression of cofilin in viable cells induced bu...

    journal_title:Cell structure and function

    pub_type: 杂志文章,评审

    doi:10.1247/csf.21.421

    authors: Yahara I,Aizawa H,Moriyama K,Iida K,Yonezawa N,Nishida E,Hatanaka H,Inagaki F

    更新日期:1996-10-01 00:00:00

  • Ca2+ translocation across liposomal membranes enhanced by unsaturated long-chain fatty acids.

    abstract::The putative ionophoretic action of phosphatidic acid or arachidonic acid metabolites for Ca2+ has offered an attractive explanation for stimulation-coupled mobilization of cytoplasmic Ca2+. We have examined the effects of Ca2+ ionophore and long-chain unsaturated fatty acids on the translocation of Ca2+ across the li...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.10.177

    authors: Utsumi K,Nobori K,Miyahara M

    更新日期:1985-06-01 00:00:00

  • Retinobenzoic acids and nuclear retinoic acid receptors.

    abstract::Retinoids (retinoic acid and its analogs) are widely involved in the control of cell proliferation, cell differentiation and embryonic development. A series of potent and novel synthetic retinoids named retinobenzoic acids has been developed. Retinobenzoic acids have proven to be useful tools in the investigation of t...

    journal_title:Cell structure and function

    pub_type: 杂志文章,评审

    doi:10.1247/csf.16.113

    authors: Hashimoto Y

    更新日期:1991-04-01 00:00:00

  • Total internal reflection fluorescence microscopy for single-molecule imaging in living cells.

    abstract::Marvelous background rejection in total internal reflection fluorescence microscopy (TIR-FM) has made it possible to visualize single-fluorophores in living cells. Cell signaling proteins including peptide hormones, membrane receptors, small G proteins, cytoplasmic kinases as well as small signaling compounds have bee...

    journal_title:Cell structure and function

    pub_type: 杂志文章,评审

    doi:10.1247/csf.27.357

    authors: Sako Y,Uyemura T

    更新日期:2002-10-01 00:00:00

  • An altered nuclear migration into the daughter bud is induced by the cyclin A1-mediated Cdc28 kinase through an aberrant spindle movement in Saccharomyces cerevisiae.

    abstract::A strain of Saccharomyces cerevisiae that contains an integrated copy of a Xenopus cyclin A1 gene under the control of the GAL1 promoter has been constructed. On inducing expression of cyclin A1, the nuclear migration that occurs prior to division becomes aberrant. Instead of migrating to the neck between the mother c...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.22.465

    authors: Sikder H,Funakoshi M,Nishimoto T,Kobayashi H

    更新日期:1997-08-01 00:00:00

  • Ganglioside composition of astrocytes.

    abstract::Short-term and long-term (greater than 7 months) cultured astrocytes from 14-day-old rat brain were analyzed for ganglioside content. Analysis of the extracted gangliosides by HPTLC revealed that ganglioside GM1 was absent in 35 days and 235 days cultured astrocytes, and that the predominant ganglioside was GM3, showi...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.14.561

    authors: Asou H,Hirano S,Uyemura K

    更新日期:1989-10-01 00:00:00

  • Ultrastructural studies on the Ca2+ localization in the dividing cells of the maize root tip.

    abstract::The distribution of Ca2+ in dividing cells of the maize root tip was examined by potassium pyroantimonate precipitation and EGTA treatment methods. Ca2+ was found in most of the cell organelles, such as the matrix of the mitochondria, the thylakoid membrane of the proplastid and the Golgi vesicles, and on the plasma m...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.18.389

    authors: Sakai-Wada A,Yagi S

    更新日期:1993-12-01 00:00:00

  • Distribution among tissues and intracellular localization of cofilin, a 21kDa actin-binding protein.

    abstract::Cofilin, a 21kDa actin-binding protein, binds to F-actin in a 1:1 molar ratio of cofilin to actin molecule (Nishida, E., S. Maekawa, and H. Sakai, Biochemistry, 23, 5307-5313, 1984) and is capable of controlling actin polymerization and depolymerization in vitro in a pH-sensitive manner (Yonezawa, N., E. Nishida, and ...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.12.443

    authors: Yonezawa N,Nishida E,Koyasu S,Maekawa S,Ohta Y,Yahara I,Sakai H

    更新日期:1987-10-01 00:00:00

  • The WD40 Domain of HIRA Is Essential for RI-nucleosome Assembly in Xenopus Egg Extracts.

    abstract::Histone chaperones are a group of histone-binding proteins that facilitate the assembly of nucleosomes, the fundamental structural units of chromatin in eukaryotes. In nucleosome assembly, deposition of a histone H3-H4 tetramer onto DNA is the first and critical step, which is mediated by the histone chaperones HIRA a...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.17001

    authors: Zhu R,Iwabuchi M,Ohsumi K

    更新日期:2017-01-01 00:00:00

  • Intracellular transport of albumin through the secretory apparatus of rat liver parenchymal cells. An immunocytochemical study.

    abstract::The fine structural localization of albumin in rat liver parenchymal cells was determined by an improved immunocytochemical method and serial sectioning. Albumin in the secretory apparatus of the parenchymal cells was present in segments of the rough endoplasmic reticulum, interrupted with negative segments, in transp...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.12.251

    authors: Yokota S,Fahimi DH

    更新日期:1987-06-01 00:00:00

  • Thrombolytic properties of an inactive proenzyme form of human urokinase secreted from human kidney cells.

    abstract::The relative fibrin-binding, fibrinolytic and fibrinogenolytic properties of single-chain pro-urokinase, an inactive proenzyme form of human urokinase purified from cultured human kidney cells, and urokinase were compared. The affinity of single-chain pro-urokinase for fibrin was much higher than that of urokinase. In...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.10.151

    authors: Kasai S,Arimura H,Nishida M,Suyama T

    更新日期:1985-06-01 00:00:00

  • Isolation of desmosomes from the epidermis of Xenopus laevis and immunochemical characterization of the Xenopus desmosomal cadherins.

    abstract::Here we report a new method of isolating epidermal desmosomes from Xenopus laevis, and a major constituent of desmosomes designated as Xenopus desmogleins (XDsg). Isolation of desmosomes from Xenopus laevis epidermis was carried out by a two step-incubation with different concentrations of NP-40. After discontinuous s...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.29.17

    authors: Ohga R,Shida M,Shida H

    更新日期:2004-02-01 00:00:00

  • Nuclear translocation of the catalytic component of DNA-dependent protein kinase upon growth stimulation in normal human T lymphocytes.

    abstract::DNA-dependent protein kinase (DNA-PK), consisting of the 470-kDa catalytic component (DNA-PKcs) and the DNA-binding regulatory component Ku protein (p70/p80), catalyzes phosphorylation of a variety of DNA replication/transcription/repair factors in the presence of double-stranded DNA. In the resting states of human pe...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.22.585

    authors: Nagasawa M,Watanabe F,Suwa A,Yamamoto K,Tsukada K,Teraoka H

    更新日期:1997-12-01 00:00:00

  • Immunocytochemical evidence for centrosomal phosphoproteins in mitotic sea urchin eggs.

    abstract::The change in distribution of centrosomal phosphoproteins was examined in sea urchin eggs from fertilization to the first cleavage by immunofluorescence staining with the anti-phosphoprotein antibodies, MPM-1 and MPM-2. The antibodies reacted with female pronuclei in unfertilized eggs as well as centriolar complexes l...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.15.13

    authors: Kuriyama R,Rao PN,Borisy GG

    更新日期:1990-02-01 00:00:00

  • Inhibition of cell proliferation by a unique lysophosphatidic acid, PHYLPA, isolated from Physarum polycephalum: signaling events of antiproliferative action by PHYLPA.

    abstract::The unique Physarum lysophosphatidic acid, PHYLPA, having a cyclopropane in the fatty acid moiety and a cyclic phosphate at C-2 and C-3 positions of the glycerol, inhibited proliferation of human fibroblast cells, TIG-3 and TIG-7, which were cultured in a chemically defined (serum-free) medium. The cells at S- and M-p...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.18.363

    authors: Murakami-Murofushi K,Kaji K,Kano K,Fukuda M,Shioda M,Murofushi H

    更新日期:1993-10-01 00:00:00

  • Developmental changes of synapsin I subcellular localization in rat cerebellar neurons.

    abstract::Synapsin I, one of the major synaptic proteins, is thought to associate with synaptic vesicles and to play a regulatory role in neurotransmitter release. In mature neurons, it is concentrated almost exclusively in presynaptic nerve endings. Here, we studied the subcellular localization of synapsin I during the develop...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.15.329

    authors: Harada A,Sobue K,Hirokawa N

    更新日期:1990-12-01 00:00:00

  • Light-independent and dependent phases of proplastid development in Euglena gracilis W3BUL.

    abstract::Cells of Euglena gracilis Klebs var. bacillaris Cori mutant W3BUL grown in darkness on Hutner's pH 3.5 medium without agitation accumulate wax ester. These cells have undeveloped proplastid remnants characteristic of this mutant. If these cells are transferred to an inorganic medium and bubbled with 2-3% CO2 in air, t...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.12.453

    authors: Osafune T,Schiff JA,Hase E

    更新日期:1987-10-01 00:00:00

  • Temporal increase of two proteins in PC-12 pheochromocytoma cells induced by nerve growth factor.

    abstract::Two-dimensional gel electrophoresis revealed two protein spots, a (molecular weight 70,000, pI 4.6) and b (molecular weight 69,000, pI 4.4) in PC-12 cells (rat pheochromocytoma cells). When the cells were induced to differentiate with nerve growth factor, the amount of protein in spot a, and later spot b increased wit...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.12.127

    authors: Noguchi S,Muramatsu T,Koike T

    更新日期:1987-02-01 00:00:00

  • Chondroitin sulfate immobilized onto culture substrates modulates DNA synthesis, tyrosine aminotransferase induction, and intercellular communication in primary rat hepatocytes.

    abstract::Newly prepared phosphatidylethanolamine (PE) conjugates of glycosaminoglycans (GAGs) enabled us to immobilize GAGs to solid phase through hydrophobic interaction. Using these compounds, called GAG-PEs, we studied the effects of GAGs immobilized to culture plates coated with various extracellular matrix (ECM) proteins ...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.20.199

    authors: Kato S,Sugiura N,Kimata K,Kujiraoka T,Toyoda J,Akamatsu N

    更新日期:1995-06-01 00:00:00

  • p18/Lamtor1-mTORC1 Signaling Controls Development of Mucin-producing Goblet Cells in the Intestine.

    abstract::Mechanistic target of rapamycin complex 1 (mTORC1) plays a pivotal role in controlling cell growth and metabolism in response to nutrients and growth factors. The activity of mTORC1 is dually regulated by amino acids and growth factor signaling, and amino acid-dependent mTORC1 activity is regulated by mTORC1 interacti...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.20018

    authors: Ito S,Nada S,Yamazaki D,Kimura T,Kajiwara K,Miki H,Okada M

    更新日期:2020-01-01 00:00:00

  • Regional variation and function of nucleotide and amino acid sequence.

    abstract::Two methods of qualitative analysis of sequence distribution in DNA and protein are presented. The first method is based on the finding that the frequency of occurrence of each nucleotide in a defined sequence with functional significance more or less deviates from uniform distribution. The deviation found in this def...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.13.387

    authors: Kiho Y

    更新日期:1988-10-01 00:00:00

  • Binding of recombinant human cytokeratin 19 to laminin: a possible role in interaction between intermediate filament derived from epithelial cells and extracellular matrixes.

    abstract::Cytokeratin 8 (CK8) and cytokeratin 19 (CK19) is a specific cytoskeletal component of simple epithelia, including bronchial epithelial cells. We hypothesized that CK8 or CK19 released from epithelial cells may bind to and cause damage to extracellular matrixes through binding of anti-CK8 or anti-CK19 autoantibodies. I...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.25.171

    authors: Dobashi N,Fujita J,Murota M,Ohtsuki Y,Bandoh S,Ueda Y,Dohmoto K,Hojo S,Nishioka M,Ishida T,Takahara J

    更新日期:2000-06-01 00:00:00

  • Bypass of the ts block of tsJT60, a G0-specific ts mutant from rat fibroblasts, by fetal bovine serum and epidermal growth factor.

    abstract::tsJT60, a temperature-sensitive (ts) G0-mutant cell line from a Fischer rat, grows normally in the exponential growth phase at 34 degrees C and 39.5 degrees C, but when stimulated with fetal bovine serum (FBS), from the G0 phase they reenter the S phase at 34 degrees C but not at 39.5 degrees C. The ts-block was bypas...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.12.421

    authors: Ninomiya-Tsuji J,Nakahara Y,Ito C,Akiyama T,Ishibashi S,Ide T

    更新日期:1987-10-01 00:00:00

  • Interleukin 2 promotes expression of an interleukin 2 receptor and proliferation of T cells stimulated with non-mitogenic dose of concanavalin A.

    abstract::Interleukin 2 (IL-2) initiated proliferation of the cells of T-enriched population stimulated with non-mitogenic dose of concanavalin A (Con A), whereas it could not induce proliferation of the cells treated with non-mitogenic lectin wheat germ agglutinin (WGA). Use of monoclonal antibody (MAb) directed against interl...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.14.193

    authors: Komada H,Nakabayashi H,Hara M,Hara M,Takahashi T,Morita J,Yoshida T,Takanari H,Izutsu K

    更新日期:1989-04-01 00:00:00

  • Expression of a 66-kD heat shock protein associated with the process of cyst formation of a true slime mold, Physarum polycephalum.

    abstract::Under unfavorable conditions for growth, haploid myxoamoebae of Physarum polycephalum retracted their pseudopodia and changed their cell shape into disk-like form, after which they constructed the cell walls to form microcysts. These morphological changes of haploid cells were associated with changes in intracellular ...

    journal_title:Cell structure and function

    pub_type: 杂志文章

    doi:10.1247/csf.17.301

    authors: Shimada Y,Kasakura T,Yokota M,Miyata Y,Murofushi H,Sakai H,Yahara I,Murakami-Murofushi K

    更新日期:1992-10-01 00:00:00