A role of cofilin/destrin in reorganization of actin cytoskeleton in response to stresses and cell stimuli.

abstract：:DNA-dependent protein kinase (DNA-PK), consisting of the 470-kDa catalytic component (DNA-PKcs) and the DNA-binding regulatory component Ku protein (p70/p80), catalyzes phosphorylation of a variety of DNA replication/transcription/repair factors in the presence of double-stranded DNA. In the resting states of human pe...

journal_title：Cell structure and function

authors： Nagasawa M,Watanabe F,Suwa A,Yamamoto K,Tsukada K,Teraoka H

更新日期：1997-12-01 00:00:00

abstract：:In this study, we measured hydrogen peroxide (H2O2) release as one of the functions of mature eosinophils, and utilized it as a quantitative index. We demonstrated that 1) the human eosinophilic leukemia cell line, EoL-1, did not release H2O2 when stimulated with phorbol myristate acetate (PMA), but after culturing wi...

journal_title：Cell structure and function

authors： Ohtsu H,Yamauchi K,Yoshie O,Tanno Y,Saito H,Hayashi N,Takishima T

更新日期：1993-04-01 00:00:00

abstract：:tsJT60, a temperature-sensitive (ts) G0-mutant cell line from a Fischer rat, grows normally in the exponential growth phase at 34 degrees C and 39.5 degrees C, but when stimulated with fetal bovine serum (FBS), from the G0 phase they reenter the S phase at 34 degrees C but not at 39.5 degrees C. The ts-block was bypas...

journal_title：Cell structure and function

authors： Ninomiya-Tsuji J,Nakahara Y,Ito C,Akiyama T,Ishibashi S,Ide T

更新日期：1987-10-01 00:00:00

abstract：:A large number of Rab/Ypt-family small GTPases have been identified from higher plants. While some of them can complement yeast ypt mutants, the expression of Arabidopsis Ara4 protein aggravated the growth defect of a subset of ypt mutants, probably because of the titration of common regulator(s) of yeast Ypt proteins...

journal_title：Cell structure and function

authors： Matsuda N,Ueda T,Sasaki Y,Nakano A

更新日期：2000-02-01 00:00:00

abstract：:Myofibril assembly requires the cell to join diverse components, correctly oriented to the rest of the cell. Titin, a huge elastic protein with a role in myogenesis, assembles during translation in vivo and may require spatially organized mRNA to allow assembly. By immunofluorescence, we examined titin and myosin prot...

journal_title：Cell structure and function

authors： Fulton AB,Alftine C

更新日期：1997-02-01 00:00:00

abstract：:It was recently reported that intravenous administration of phorbol 12-myristate 13-acetate (PMA) showed a therapeutic effect in myelocytic leukemia patients. However, we previously observed that, in serum-free conditions, polymorphonuclear leucocytes (PMNs) were killed rapidly by exposure to PMA, suggesting the possi...

journal_title：Cell structure and function

authors： Suzuki K,Kato H,Sakuma Y,Namiki H

更新日期：2001-08-01 00:00:00

abstract：:Caffeine has been shown to enhance the lethal effect of DNA-damaging agents in mammalian cells, and the potentiation by caffeine of this effect is generally interpreted as the result of inhibition by caffeine of the repair of damaged DNA. However, the mechanism by which caffeine enhances the lethal effect of DNA-damag...

journal_title：Cell structure and function

authors： Ishida R,Kozaki M,Takahashi T

更新日期：1985-12-01 00:00:00

abstract：:The amount of intact 40S rRNA precursor was followed by Northern hybridization in the course of the early embryogenesis of wild-type Xenopus laevis and its anucleolate mutant. The total amount of 40S rRNA precursor did not alter appreciably until the midblastula stage, decreased at the late blastula stage, and then in...

journal_title：Cell structure and function

authors： Furuno N,Shiokawa K,Kobayashi H

更新日期：1988-04-01 00:00:00

abstract：:A granulocyte colony-stimulating factor (G-CSF) was highly purified from the serum-free culture medium of RSP -2 X P3 cells. The G-CSF had an apparent molecular weight of 33,000 as determined by high speed gel permeation chromatography, but its molecular weight was decreased to 15,000 by 0.1% sodium dodecyl sulfate. A...

journal_title：Cell structure and function

authors： Tsuneoka K,Shikita M

更新日期：1984-03-01 00:00:00

abstract：:Previously, we serially transplanted tumors that produced colony-stimulating factor (CSF) into nude mice, who developed marked granulocytosis along with tumor growth; their leukocyte counts reaching approximately one million per cu mm. The numbers of CFU-GM, CFU-E, CFU-Meg, CFU-S and BFU-E were increased in nude mice ...

journal_title：Cell structure and function

authors： Takeda K,Suda T,Sudo T,Miura Y

更新日期：1987-06-01 00:00:00

abstract：:Epidermal growth factor (EGF) was derivatized at the amino terminus with N-succinimidyl 3-(2-pyridyldithio)propionate and then cross-linked to the cysteinyl residues of alpha 2-macroglobulin (alpha 2M) via disulfide bonds. The EGF-alpha 2M conjugate delivered EGF into dense lysosomal fractions through binding to alpha...

journal_title：Cell structure and function

authors： Ito F,Ito S,Shimizu N

更新日期：1984-06-01 00:00:00

abstract：:NBT-II cells on collagen-coated substrates move rapidly and persistently, maintaining a semi-circular shape with a large lamellipodium, in a manner similar to fish keratocytes. The inhibitor of phospholipase D (PLD), n-butanol, completely blocked the migration and disturbed the characteristic localization of actin alo...

journal_title：Cell structure and function

authors： Nagasaki A,Inotsume K,Kanada M,Uyeda TQ

更新日期：2008-01-01 00:00:00

abstract：:Hepatic stellate cells (HSCs) were changed in their morphology, proliferative activity, and functions by culturing on type I collagen gel, as compared to the culture on polystyrene surface. HSCs have been found to produce extracellular matrix components and matrix metalloproteinases (MMPs). In this study, we have asse...

journal_title：Cell structure and function

authors： Li YL,Sato M,Kojima N,Miura M,Senoo H

更新日期：1999-10-01 00:00:00

abstract：:Retinoids (retinoic acid and its analogs) are widely involved in the control of cell proliferation, cell differentiation and embryonic development. A series of potent and novel synthetic retinoids named retinobenzoic acids has been developed. Retinobenzoic acids have proven to be useful tools in the investigation of t...

journal_title：Cell structure and function

authors： Hashimoto Y

更新日期：1991-04-01 00:00:00

abstract：:Autophagy is a starvation response in eukaryotes by which the cell delivers cytoplasmic components to the vacuole for degradation, and is mediated by a double membrane structure called the autophagosome. We have previously proposed that the specific combination of COPII like components, including Sec24p, is required f...

journal_title：Cell structure and function

authors： Hamasaki M,Noda T,Ohsumi Y

更新日期：2003-02-01 00:00:00

abstract：:Cytokeratin 8 (CK8) and cytokeratin 19 (CK19) is a specific cytoskeletal component of simple epithelia, including bronchial epithelial cells. We hypothesized that CK8 or CK19 released from epithelial cells may bind to and cause damage to extracellular matrixes through binding of anti-CK8 or anti-CK19 autoantibodies. I...

journal_title：Cell structure and function

authors： Dobashi N,Fujita J,Murota M,Ohtsuki Y,Bandoh S,Ueda Y,Dohmoto K,Hojo S,Nishioka M,Ishida T,Takahara J

更新日期：2000-06-01 00:00:00

abstract：:The involvement of actin filaments from the host cell on the process of invasion of trypomastigote forms of Trypanosma cruzi was analyzed in seven different cell lines. Prior incubation of all cell lines with cytochalasin D, under conditions which interfere with actin filaments, markedly inhibited parasite internaliza...

journal_title：Cell structure and function

authors： Rosestolato CT,Dutra Jda M,De Souza W,de Carvalho TM

更新日期：2002-04-01 00:00:00

abstract：:Tritrichomonas foetus strongly agglutinates human erythrocytes, thus suggesting the occurrence of an adhesin associated with its surface. Adherence was observed immediately after mixing of the parasites with erythrocytes and the intensity increased for up to 30 min. Scanning electron microscopy examination of T. foetu...

journal_title：Cell structure and function

authors： da Silva NS,Dias Filho BP,De Souza W

更新日期：1996-08-01 00:00:00

abstract：:We have previously shown that mouse L.P3 cells secrete fibronectin and a novel protein, gelatin-non-binding and heparin-binding cell-adhesive protein (GNCP). Here, we screened and characterized cell-adhesive proteins in the conditioned media (CM's) of a series of cell lines growing in a protein- and lipid-free synthet...

journal_title：Cell structure and function

authors： Morita H,Yasumitsu H,Watanabe Y,Miyazaki K,Umeda M

更新日期：1993-02-01 00:00:00

abstract：:DNA synthesis of WF-1 fibroblasts derived from a patient with Werner's syndrome was stimulated by fetal calf serum and adult human serum but not by various mitogens including epidermal growth factor, platelet-derived growth factor (PDGF), fibroblast growth factor, insulin and 12-O-tetradecanoylphorbol-13-acetate (TPA)...

journal_title：Cell structure and function

authors： Hirai M,Amagai M,Tajima S,Nishikawa T,Shimizu N

更新日期：1988-12-01 00:00:00

abstract：:Recent studies have revealed various biological functions for repetitive sequences, which make up about half of the human genome. One such sequence, major satellites, which are tandem repetitive sequences adjacent to the centromere, have been shown to be a kinetochore component that plays a role in the formation and f...

journal_title：Cell structure and function

authors： Kishi Y,Kondo S,Gotoh Y

更新日期：2012-01-01 00:00:00

abstract：:In view of the importance of vitamin A in the human immune system and the central role of interleukin-2 (IL-2) in the proliferation of T-lymphocytes, we examined the effects of all-trans-retinoic acid (ATRA) on the protein and gene expression of IL-2 in the human T-cell line HUT-78 when stimulated with either 12-O-tet...

journal_title：Cell structure and function

authors： Goto S,Okada N,Kaneko A,Isemura M

更新日期：2008-01-01 00:00:00

abstract：:To investigate the mechanism of myogenic differentiation, we are using quail myoblast cells (QM cells) transformed with a temperature-sensitive mutant of Rous sarcoma virus (ts-RSV), termed QM-RSV cells. At 35.5 degrees C, a permissive temperature for RSV, QM-RSV cells repeatedly proliferate without differentiation, b...

journal_title：Cell structure and function

authors： Hase H,Hirayama E,Kim J

更新日期：1996-02-01 00:00:00

abstract：:Checkpoint kinase 1 (Chk1) is a conserved protein kinase central to the cell-cycle checkpoint during DNA damage response (DDR). Until recently, ATR, a protein kinase activated in response to DNA damage or stalled replication, has been considered as the sole regulator of Chk1. Recent progress, however, has led to the i...

journal_title：Cell structure and function

authors： Goto H,Kasahara K,Inagaki M

更新日期：2015-01-01 00:00:00

abstract：:Staurosporine has been reported to cause arrest of cells in G1 phase at low concentration and in G2 phase at high concentration. This raises the question of why the effects of staurosporine on the cell cycle depend on the applied concentration. In order to verify these multiple functions of staurosporine in Meth-A cel...

journal_title：Cell structure and function

authors： Zong ZP,Fujikawa-Yamamoto K,Li AL,Yamaguchi N,Chang YG,Murakami M,Odashima S,Ishikawa Y

更新日期：1999-12-01 00:00:00

abstract：:To study the effects of surface materials of cells on the behavior of other neighboring cells in a crowded culture, confluent sheets of rat 3Y1 fibroblasts were fixed and then 3Y1 cells were seeded on to them. Among confluent sheets unfixed, fixed with formalin and fixed with ethanol and an empty plastic dish surface,...

journal_title：Cell structure and function

authors： Okuda A,Sasaki M,Kimura G

更新日期：1990-10-01 00:00:00

abstract：:Under unfavorable conditions for growth, haploid myxoamoebae of Physarum polycephalum retracted their pseudopodia and changed their cell shape into disk-like form, after which they constructed the cell walls to form microcysts. These morphological changes of haploid cells were associated with changes in intracellular ...

journal_title：Cell structure and function

authors： Shimada Y,Kasakura T,Yokota M,Miyata Y,Murofushi H,Sakai H,Yahara I,Murakami-Murofushi K

更新日期：1992-10-01 00:00:00

abstract：:Pericentric regions form epigenetically organized, silent heterochromatin structures that accumulate histone H3 lysine 9 tri-methylation (H3K9me3) and heterochromatin protein 1 (HP1), a methylated H3K9-binding protein. At pericentric regions, Suv39h is the major enzyme that generates H3K9me3. Suv39h also interacts dir...

journal_title：Cell structure and function

authors： Muramatsu D,Kimura H,Kotoshiba K,Tachibana M,Shinkai Y

更新日期：2016-12-03 00:00:00

abstract：:tsJT60 is a nonlethal temperature-sensitive (ts) mutant of a Fischer rat cell line (3Y1) classified as a G0 mutant; i.e., the ts defect is not expressed within the cell growth cycle but is expressed only between the G0 and S phase. tsJT60 clones transformed with oncogenes such as adenovirus E1A, polyoma large T, polyo...

journal_title：Cell structure and function

authors： Tai YY,Ninomiya-Tsuji J,Furuoku K,Ogawa N,Ishibashi S,Shiroki K,Segawa K,Tsuchida N,Shibuya M,Ide T

更新日期：1990-12-01 00:00:00

abstract：:Phosphorylation of regulatory light chain (RMLC) of myosin II at Ser19/Thr18 is likely to play important roles in controlling the morphological changes seen during cell division of cultured mammalian cells. Phosphorylation of RMLC regulates the activity of myosin II, an essntial motor for cytokinesis, and phosphorylat...

journal_title：Cell structure and function

authors： Matsumura F,Totsukawa G,Yamakita Y,Yamashiro S

更新日期：2001-12-01 00:00:00