Retinobenzoic acids and nuclear retinoic acid receptors.

abstract：:Epidermal growth factor (EGF) was derivatized at the amino terminus with N-succinimidyl 3-(2-pyridyldithio)propionate and then cross-linked to the cysteinyl residues of alpha 2-macroglobulin (alpha 2M) via disulfide bonds. The EGF-alpha 2M conjugate delivered EGF into dense lysosomal fractions through binding to alpha...

journal_title：Cell structure and function

authors： Ito F,Ito S,Shimizu N

更新日期：1984-06-01 00:00:00

abstract：:A method has been developed to isolate pure preparations of nuclei in high yield from commercially available viable rice embryos (germ), employing extraction with buffer solution containing glycerol (without detergent) and polyamine, followed by centrifugation on a 30% Percoll cushion. The intactness of the isolated n...

journal_title：Cell structure and function

authors： Yamaguchi J,Lim PY,Aratani K,Akazawa T

更新日期：1992-04-01 00:00:00

abstract：:Primary hepatocytes were cultured on collagen gel in serum-free, alpha-modified Eagle's minimum essential medium containing 0.1 microM insulin, 0.1 microM dexamethasone, 10 mM pyruvate and supplements such as glucagon, epinephrine or growth hormone. The activities of alkaline phosphatase, 5'-nucleotidase and gamma-glu...

journal_title：Cell structure and function

authors： Horiuti Y,Yano K,Shibuya Y

更新日期：1993-02-01 00:00:00

abstract：:To elucidate the cytotoxic mechanism of tumor necrosis factor (TNF), we isolated TNF-resistant sublines of L929 cells. As compared with L929 cells, TNF-resistant cells retained similar number and affinity of TNF-binding sites, and showed a similar growth rate. TNF stimulated arachidonate release from L929 cells, while...

journal_title：Cell structure and function

authors： Hayakawa M,Oku N,Takagi T,Hori T,Shibamoto S,Yamanaka Y,Takeuchi K,Tsujimoto M,Ito F

更新日期：1991-08-01 00:00:00

abstract：:In view of the importance of vitamin A in the human immune system and the central role of interleukin-2 (IL-2) in the proliferation of T-lymphocytes, we examined the effects of all-trans-retinoic acid (ATRA) on the protein and gene expression of IL-2 in the human T-cell line HUT-78 when stimulated with either 12-O-tet...

journal_title：Cell structure and function

authors： Goto S,Okada N,Kaneko A,Isemura M

更新日期：2008-01-01 00:00:00

abstract：:The fine structural localization of albumin in rat liver parenchymal cells was determined by an improved immunocytochemical method and serial sectioning. Albumin in the secretory apparatus of the parenchymal cells was present in segments of the rough endoplasmic reticulum, interrupted with negative segments, in transp...

journal_title：Cell structure and function

authors： Yokota S,Fahimi DH

更新日期：1987-06-01 00:00:00

abstract：:The localization of mRNA in neuronal dendrites plays a role in both locally and temporally regulated protein synthesis, which is required for certain forms of synaptic plasticity. RNA granules constitute a dendritic mRNA transport machinery in neurons, which move along microtubules. RNA granules contain densely packed...

journal_title：Cell structure and function

authors： Miki T,Takano K,Yoneda Y

更新日期：2005-01-01 00:00:00

abstract：:The effects of heavy ion microbeams on muscle fibers isolated from mouse skeletal muscles were examined by electron microscopy. The plasma membranes of heavy ion beam-irradiated areas of muscle fibers showed irregular protrusions and invaginations. In the cytoplasm, an irregular distribution of microfilaments was foun...

journal_title：Cell structure and function

authors： Hino M,Wada S,Tajika Y,Morimura Y,Hamada N,Funayama T,Sakashita T,Kakizaki T,Kobayashi Y,Yorifuji H

更新日期：2007-01-01 00:00:00

abstract：:The putative ionophoretic action of phosphatidic acid or arachidonic acid metabolites for Ca2+ has offered an attractive explanation for stimulation-coupled mobilization of cytoplasmic Ca2+. We have examined the effects of Ca2+ ionophore and long-chain unsaturated fatty acids on the translocation of Ca2+ across the li...

journal_title：Cell structure and function

authors： Utsumi K,Nobori K,Miyahara M

更新日期：1985-06-01 00:00:00

abstract：:A strain of Saccharomyces cerevisiae that contains an integrated copy of a Xenopus cyclin A1 gene under the control of the GAL1 promoter has been constructed. On inducing expression of cyclin A1, the nuclear migration that occurs prior to division becomes aberrant. Instead of migrating to the neck between the mother c...

journal_title：Cell structure and function

authors： Sikder H,Funakoshi M,Nishimoto T,Kobayashi H

更新日期：1997-08-01 00:00:00

abstract：:Significant progress has been made toward our understanding of the basic principle of nucleocytoplasmic transport, and the structure of transport factors, as well as the diversity of nucleocytoplasmic transport pathways. This review outlines the current knowledge of transport, and discusses the problems that remain as...

journal_title：Cell structure and function

authors： Imamoto N

更新日期：2000-08-01 00:00:00

abstract：:Actin, a major component of microfilaments, is involved in various eukaryotic cellular functions. Over the past two decades, actin fused with fluorescent protein has been used as a probe to detect the organization and dynamics of the actin cytoskeleton in living eukaryotic cells. It is generally assumed that the expre...

journal_title：Cell structure and function

authors： Nagasaki A,T Kijima S,Yumoto T,Imaizumi M,Yamagishi A,Kim H,Nakamura C,Q P Uyeda T

更新日期：2017-01-01 00:00:00

abstract：:When spores of Dictyostelium discoideum matured through normal development were stained with rhodamine-phalloidin, rod-shaped fluorescence was found across the nucleus and in the cytoplasm. Electron micrographs of the rod shape showed that it consisted of long and straight fibers 12 nm in diameter. In contrast, a conv...

journal_title：Cell structure and function

authors： Sameshima M,Chijiiwa Y,Kishi Y,Hashimoto Y

更新日期：1994-08-01 00:00:00

abstract：:Hepatic stellate cells (HSCs), a mesenchymal cell type in hepatic parenchyma, have unique features with respect to their cellular origin, morphology, and function. Normal, quiescent HSCs function as major vitamin A-storing cells containing over 80% of total vitamin A in the body to maintain vitamin A homeostasis. HSCs...

journal_title：Cell structure and function

authors： Sato M,Suzuki S,Senoo H

更新日期：2003-04-01 00:00:00

abstract：:Stimulation of polymorphonuclear leukocytes (PMN) by tetravalent concanavalin A (alpha-ConA) induces membrane depolarization preceding the onset of superoxide anion (O2-) production. Both divalent and monovalent ConA analogues were studied to evaluate the role of valence. Monovalent ConA (m-ConA) was inactive in stimu...

journal_title：Cell structure and function

authors： Kuroki M,Kamo N,Kobatake Y,Abe Y,Ishii S

更新日期：1986-06-01 00:00:00

abstract：:Tenascin-C is an oligomeric glycoprotein of the extracellular matrix that has been found to have both adhesive and anti-adhesive properties for cells. Recent elucidation of the two major TNC splice variants (320 kDa and 220 kDa) has shed light on the possibility of varying functions of the molecule based on its splici...

journal_title：Cell structure and function

authors： Ghert MA,Qi WN,Erickson HP,Block JA,Scully SP

更新日期：2001-06-01 00:00:00

abstract：:It has been shown that in PC12 and its subclone PC12h treatment of the cells with nerve growth factor (NGF) induces a selective decrease in the incorporation of radioactive phosphate into a 100,000-dalton protein, designated in an earlier study as Nsp100, in the subsequent phosphorylation of soluble extracts from cell...

journal_title：Cell structure and function

authors： Hashimoto S,Hagino A

更新日期：1989-02-01 00:00:00

abstract：:The morphology and intramembranous particle (IMP) distribution of spermatozoa were investigated in four species of cyprinid fishes, Gnathopogon elongatus elongatus, Pseudogobio esocinus, Acheilognathus lanceolatus and Puntius tetrazona. In particular, observation of IMP distribution was conducted as an aspect of compa...

journal_title：Cell structure and function

authors： Ohta T,Matsuda M

更新日期：1995-08-01 00:00:00

abstract：:CD9 and CD63 belong to a tetramembrane-spanning glycoprotein family called tetraspanin, and are involved in a wide variety of cellular processes, but the structure-function relationship of this family of proteins has yet to be clarified. CD9 associates with diphtheria toxin receptor (DTR), which is identical to the me...

journal_title：Cell structure and function

authors： Ryu F,Takahashi T,Nakamura K,Takahashi Y,Kobayashi T,Shida S,Kameyama T,Mekada E

更新日期：2000-10-01 00:00:00

abstract：:It has been suggested that the organization of microtubules during mitosis plays an important role in cytokinesis in animal cells. We studied the organization of microtubules during the first cleavage and its role in cytokinesis of Xenopus eggs. First, we examined the immunofluorescent localization of microtubules in ...

journal_title：Cell structure and function

authors： Takayama M,Noguchi T,Yamashiro S,Mabuchi I

更新日期：2002-08-01 00:00:00

abstract：:DNA-dependent protein kinase (DNA-PK), consisting of the 470-kDa catalytic component (DNA-PKcs) and the DNA-binding regulatory component Ku protein (p70/p80), catalyzes phosphorylation of a variety of DNA replication/transcription/repair factors in the presence of double-stranded DNA. In the resting states of human pe...

journal_title：Cell structure and function

authors： Nagasawa M,Watanabe F,Suwa A,Yamamoto K,Tsukada K,Teraoka H

更新日期：1997-12-01 00:00:00

abstract：:DNA synthesis of WF-1 fibroblasts derived from a patient with Werner's syndrome was stimulated by fetal calf serum and adult human serum but not by various mitogens including epidermal growth factor, platelet-derived growth factor (PDGF), fibroblast growth factor, insulin and 12-O-tetradecanoylphorbol-13-acetate (TPA)...

journal_title：Cell structure and function

authors： Hirai M,Amagai M,Tajima S,Nishikawa T,Shimizu N

更新日期：1988-12-01 00:00:00

abstract：:Vascular endothelial cells possess antithrombotic properties, which are determined by the balance between plasminogen activators (PAs) and PA inhibitors (PAls). A cell line, TKM-33, has been established and cloned from human umbilical vein endothelial cells, was previously reported to produce a large amount of urokina...

journal_title：Cell structure and function

authors： Ueshima S,Matsumoto H,Izaki S,Mitsui Y,Fukao H,Okada K,Matsuo O

更新日期：1999-04-01 00:00:00

abstract：:tsJT60, a temperature-sensitive (ts) G0-mutant cell line from a Fischer rat, grows normally in the exponential growth phase at 34 degrees C and 39.5 degrees C, but when stimulated with fetal bovine serum (FBS), from the G0 phase they reenter the S phase at 34 degrees C but not at 39.5 degrees C. The ts-block was bypas...

journal_title：Cell structure and function

authors： Ninomiya-Tsuji J,Nakahara Y,Ito C,Akiyama T,Ishibashi S,Ide T

更新日期：1987-10-01 00:00:00

abstract：:Hepatic stellate cells (HSCs) were changed in their morphology, proliferative activity, and functions by culturing on type I collagen gel, as compared to the culture on polystyrene surface. HSCs have been found to produce extracellular matrix components and matrix metalloproteinases (MMPs). In this study, we have asse...

journal_title：Cell structure and function

authors： Li YL,Sato M,Kojima N,Miura M,Senoo H

更新日期：1999-10-01 00:00:00

abstract：:Nonmuscle myosin II activity is regulated by phosphorylation of the myosin II regulatory light chain (MRLC) at Ser19 or at both Thr18 and Ser19, and the phosphorylation of MRLC promotes the contractility and stability of actomyosin. To analyze the states of MRLC phosphorylation at the leading edge in the motile HeLa c...

journal_title：Cell structure and function

authors： Uchimura T,Fumoto K,Yamamoto Y,Ueda K,Hosoya H

更新日期：2002-12-01 00:00:00

abstract：:Genomics and proteomics studies in Chlamydomonas have revealed that an axoneme is composed of 200-600 types of proteins, including uncharacterized proteins collectively named flagellar-associated proteins (FAPs). Nine FAPs contain the EF-hand motif; however, they have not yet been well characterized. To find component...

journal_title：Cell structure and function

authors： Kirima J,Oiwa K

更新日期：2018-02-16 00:00:00

abstract：:Mechanistic target of rapamycin complex 1 (mTORC1) plays a pivotal role in controlling cell growth and metabolism in response to nutrients and growth factors. The activity of mTORC1 is dually regulated by amino acids and growth factor signaling, and amino acid-dependent mTORC1 activity is regulated by mTORC1 interacti...

journal_title：Cell structure and function

authors： Ito S,Nada S,Yamazaki D,Kimura T,Kajiwara K,Miki H,Okada M

更新日期：2020-01-01 00:00:00

abstract：:Spindle pole bodies (SPBs) attached to nuclei were isolated from Saccharomyces cerevisiae; they nucleated microtubules to form asters in vitro. This aster-forming activity was stable in 30% dimethylsulfoxide or 10% 2-propanol and could be solubilized with KCl. The KCl extract contained many protein components, which a...

journal_title：Cell structure and function

authors： Yamamoto A,Nagai K,Yamasaki M,Matsuhashi M

更新日期：1990-08-01 00:00:00

abstract：:The precise control of spindle microtubule (MT) dynamics is essential for chromosome capture and alignment. Kif18A/kinesin-8, an essential regulator of kinetochore MT dynamics, accumulates at its plus-ends in metaphase but not prometaphase cells. The underlying mechanism of time-dependent and kinetochore MT-specific p...

journal_title：Cell structure and function

authors： Masuda N,Shimodaira T,Shiu SJ,Tokai-Nishizumi N,Yamamoto T,Ohsugi M

更新日期：2011-01-01 00:00:00