Abstract:
:Spindle pole bodies (SPBs) attached to nuclei were isolated from Saccharomyces cerevisiae; they nucleated microtubules to form asters in vitro. This aster-forming activity was stable in 30% dimethylsulfoxide or 10% 2-propanol and could be solubilized with KCl. The KCl extract contained many protein components, which aggregated upon dialysis against a low concentration salt solution. When incubated with tubulin, the aggregates formed asters. Measurements of the elongation rates of the astral microtubules indicated that the microtubules were nucleated from the SPBs or from the aggregates reconstructed from the KCl extract by dialysis. The plus end was distal to the astral center, as in the case of the microtubule organizing center (MTOC) of mammalian cells. We suggest that the proteins extracted with KCl are responsible for microtubule nucleation in SPBs and that this SPB seems to have the same mechanism for microtubule nucleation as the MTOC in higher eukaryotes.
journal_name
Cell Struct Functjournal_title
Cell structure and functionauthors
Yamamoto A,Nagai K,Yamasaki M,Matsuhashi Mdoi
10.1247/csf.15.221subject
Has Abstractpub_date
1990-08-01 00:00:00pages
221-8issue
4eissn
0386-7196issn
1347-3700journal_volume
15pub_type
杂志文章abstract::In the present study, we compared the abilities of ricin and diphtheria toxin to induce apoptosis in Vero cells. The cytolysis and DNA fragmentation by ricin paralleled its protein synthesis inhibitory activity. However, unlike ricin, diphtheria toxin could induce neither cytolysis nor DNA fragmentation in Vero cells ...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.26.279
更新日期:2001-10-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.15.373
更新日期:1990-12-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.19.279
更新日期:1994-10-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.28.487
更新日期:2003-10-01 00:00:00
abstract::Cells of Euglena gracilis Klebs var. bacillaris Cori mutant W3BUL grown in darkness on Hutner's pH 3.5 medium without agitation accumulate wax ester. These cells have undeveloped proplastid remnants characteristic of this mutant. If these cells are transferred to an inorganic medium and bubbled with 2-3% CO2 in air, t...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.12.453
更新日期:1987-10-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2003-04-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.21.111
更新日期:1996-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1991-04-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.10.271
更新日期:1985-09-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.17.105
更新日期:1992-04-01 00:00:00
abstract::The Saccharomyces cerevisiae autophagy-initiation complex, Atg1 kinase complex, consists of Atg1, Atg13, Atg17, Atg29, and Atg31, while the corresponding complex in most other eukaryotes, including mammals, is composed of ULK1 (or ULK2), Atg13, FIP200 (also known as RB1CC1), and Atg101. ULKs are homologs of Atg1, and ...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
doi:10.1247/csf.15013
更新日期:2016-01-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.17.301
更新日期:1992-10-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.11.199
更新日期:1986-06-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.27.479
更新日期:2002-12-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.9.67
更新日期:1984-03-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.24.71
更新日期:1999-04-01 00:00:00
abstract::Cofilin, a 21kDa actin-binding protein, binds to F-actin in a 1:1 molar ratio of cofilin to actin molecule (Nishida, E., S. Maekawa, and H. Sakai, Biochemistry, 23, 5307-5313, 1984) and is capable of controlling actin polymerization and depolymerization in vitro in a pH-sensitive manner (Yonezawa, N., E. Nishida, and ...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.12.443
更新日期:1987-10-01 00:00:00
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journal_title:Cell structure and function
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更新日期:1984-06-01 00:00:00
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journal_title:Cell structure and function
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journal_title:Cell structure and function
pub_type: 杂志文章,评审
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更新日期:1991-04-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.07035
更新日期:2008-01-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.19.73
更新日期:1994-04-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.12.273
更新日期:1987-06-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.15001
更新日期:2015-01-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.16.323
更新日期:1991-08-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.21.17
更新日期:1996-02-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.26.235
更新日期:2001-08-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.12.127
更新日期:1987-02-01 00:00:00
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journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.14.87
更新日期:1989-02-01 00:00:00