Abstract:
:Under unfavorable conditions for growth, haploid myxoamoebae of Physarum polycephalum retracted their pseudopodia and changed their cell shape into disk-like form, after which they constructed the cell walls to form microcysts. These morphological changes of haploid cells were associated with changes in intracellular distribution of actin filaments. Staining with phalloidin showed that actin filaments were almost uniformly distributed throughout the cytoplasm of the myxoamoebae. When these cells were transferred to a cyst-inducing medium, the actin structures changed into short rods or dots, after which the rods/dots disappeared in the microcysts. An incubation of the myxoamoebae in the cyst-inducing medium caused the synthesis of several proteins, among which a 66-kD protein was most prominently induced. The morphological changes and the induction of the 66-kD protein was pronounced at elevated temperatures, e.g. 40 degrees C. The 66-kD protein was not induced, however, when plasmodia of the same species were incubated at 40 degrees C. We found that the 66-kD protein was co-precipitated with polymerized actin and bound to ATP-agarose. A double staining of the disk-shaped cells with anti-66-kD protein antibody and phalloidin revealed superimposable localization of the 66-kD protein and actin filaments in the short rods or dots. Although the induction of the 66-kD protein was enhanced at high temperatures, the protein was immunologically unrelated to the common heat shock proteins, HSP70 and HSP90, those are highly conserved during evolution. These results indicate that the 66-kD protein is a novel heat shock protein which is specifically expressed during cyst formation.
journal_name
Cell Struct Functjournal_title
Cell structure and functionauthors
Shimada Y,Kasakura T,Yokota M,Miyata Y,Murofushi H,Sakai H,Yahara I,Murakami-Murofushi Kdoi
10.1247/csf.17.301keywords:
subject
Has Abstractpub_date
1992-10-01 00:00:00pages
301-9issue
5eissn
0386-7196issn
1347-3700journal_volume
17pub_type
杂志文章abstract::DNA-dependent protein kinase (DNA-PK), consisting of the 470-kDa catalytic component (DNA-PKcs) and the DNA-binding regulatory component Ku protein (p70/p80), catalyzes phosphorylation of a variety of DNA replication/transcription/repair factors in the presence of double-stranded DNA. In the resting states of human pe...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.22.585
更新日期:1997-12-01 00:00:00
abstract::High voltage electric pulses, 1000 V/cm, were found to induce cell fusion efficiently when delivered to cells growing in a monolayer culture. The maximum yield of fused cells was about 30% of cells remaining after treatment. Colonies of interspecies hybrid cells between mouse and others also appeared with a frequency ...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.14.173
更新日期:1989-04-01 00:00:00
abstract::Primary hepatocytes were cultured on collagen gel in serum-free, alpha-modified Eagle's minimum essential medium containing 0.1 microM insulin, 0.1 microM dexamethasone, 10 mM pyruvate and supplements such as glucagon, epinephrine or growth hormone. The activities of alkaline phosphatase, 5'-nucleotidase and gamma-glu...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.18.73
更新日期:1993-02-01 00:00:00
abstract::Here, we would like to point out important milestones in the study of nuclear radial positioning and gene expression during differentiation processes. In addition, changes in the histone signature that significantly precede various differentiation pathways are reviewed. We address the regulatory functions of chromatin...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
doi:10.1247/csf.09021
更新日期:2010-01-01 00:00:00
abstract::Cholera toxin binding sites in frozen sections of rat thymus were studied by indirect immunofluorescence and were found to be localized selectively in the medulla of thymic lobules. By flow cytometric analysis, two populations of thymocytes differing in size and reactivity towards cholera toxin were detected; the smal...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.12.339
更新日期:1987-08-01 00:00:00
abstract::Actin, a major component of microfilaments, is involved in various eukaryotic cellular functions. Over the past two decades, actin fused with fluorescent protein has been used as a probe to detect the organization and dynamics of the actin cytoskeleton in living eukaryotic cells. It is generally assumed that the expre...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.17016
更新日期:2017-01-01 00:00:00
abstract::Histone chaperones are a group of histone-binding proteins that facilitate the assembly of nucleosomes, the fundamental structural units of chromatin in eukaryotes. In nucleosome assembly, deposition of a histone H3-H4 tetramer onto DNA is the first and critical step, which is mediated by the histone chaperones HIRA a...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.17001
更新日期:2017-01-01 00:00:00
abstract::In adult rat cerebellum, HPC-1/syntaxin 1 is detected at high density on the plasma membrane of the non-synaptic region of parallel fibers in addition to the synaptic terminal membranes and the synaptic vesicles (Koh, S., Yamamoto, A., Inoue, A., Inoue, Y., Akagawa, Y., Kawamura, Y., Kawamoto, Y., and Tashiro, Y. (199...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.21.525
更新日期:1996-12-01 00:00:00
abstract::Autophagy is a starvation response in eukaryotes by which the cell delivers cytoplasmic components to the vacuole for degradation, and is mediated by a double membrane structure called the autophagosome. We have previously proposed that the specific combination of COPII like components, including Sec24p, is required f...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.28.49
更新日期:2003-02-01 00:00:00
abstract::The Golgi stress response is a homeostatic mechanism that controls the capacity of the Golgi apparatus in accordance with cellular demands. When the capacity of the Golgi apparatus becomes insufficient (Golgi stress), transcription levels of Golgi-related genes encoding glycosylation enzymes, a Golgi structural protei...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.16005
更新日期:2016-07-30 00:00:00
abstract::A monoclonal type M-immunoglobulin (IgM) was generated in mice against a nuclease-urea extract of HeLa metaphase chromosomes. This antibody stains metaphase chromosomes from a variety of mammalian cultured cell types by indirect immunofluorescence. Antibody 12C7 reacts by western transfer technique with histone H1 in ...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.16.323
更新日期:1991-08-01 00:00:00
abstract::Cultured hepatic stellate cells (HSCs) are known to change their morphology and function with respect to the production of extracellular matrices (ECMs) and matrix metalloproteinases (MMPs) in response to ECM components. We examined the regulatory role of the native form of type I collagen fibrils in pro-MMP-2 product...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.28.505
更新日期:2003-12-01 00:00:00
abstract::Staurosporine has been reported to cause arrest of cells in G1 phase at low concentration and in G2 phase at high concentration. This raises the question of why the effects of staurosporine on the cell cycle depend on the applied concentration. In order to verify these multiple functions of staurosporine in Meth-A cel...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.24.457
更新日期:1999-12-01 00:00:00
abstract::Epidermal growth factor (EGF) was derivatized at the amino terminus with N-succinimidyl 3-(2-pyridyldithio)propionate and then cross-linked to the cysteinyl residues of alpha 2-macroglobulin (alpha 2M) via disulfide bonds. The EGF-alpha 2M conjugate delivered EGF into dense lysosomal fractions through binding to alpha...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.9.105
更新日期:1984-06-01 00:00:00
abstract::Retinoids (retinoic acid and its analogs) are widely involved in the control of cell proliferation, cell differentiation and embryonic development. A series of potent and novel synthetic retinoids named retinobenzoic acids has been developed. Retinobenzoic acids have proven to be useful tools in the investigation of t...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
doi:10.1247/csf.16.113
更新日期:1991-04-01 00:00:00
abstract::We constructed a fusion protein of the cell-binding domain of human fibronectin and human basic fibroblast growth factor, and prepared a polypeptide with both cell-adhesive activity and growth factor activity. A human gene fragment coding for basic fibroblast growth factor was amplified by the polymerase chain reactio...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.19.37
更新日期:1994-02-01 00:00:00
abstract::In this study, we measured hydrogen peroxide (H2O2) release as one of the functions of mature eosinophils, and utilized it as a quantitative index. We demonstrated that 1) the human eosinophilic leukemia cell line, EoL-1, did not release H2O2 when stimulated with phorbol myristate acetate (PMA), but after culturing wi...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.18.125
更新日期:1993-04-01 00:00:00
abstract::Tenascin-C is an oligomeric glycoprotein of the extracellular matrix that has been found to have both adhesive and anti-adhesive properties for cells. Recent elucidation of the two major TNC splice variants (320 kDa and 220 kDa) has shed light on the possibility of varying functions of the molecule based on its splici...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.26.179
更新日期:2001-06-01 00:00:00
abstract::Upon tryptic digestion of synaptosomes, ATPase activities decreased in the order of Cl(-)-ATPase greater than or equal to Na+,K+-ATPase greater than anion-insensitive Mg2+-ATPase. Upon synaptosome treatment with hypotonic solution, Cl(-)-ATPase or anion-insensitive Mg2+-ATPase was slightly inactivated, while Na+,K+-AT...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.13.105
更新日期:1988-04-01 00:00:00
abstract::Histone modifications play critical roles in the epigenetic regulation of gene expression and in the maintenance of genome integrity. Acetylation and methylation of histone H3 are particularly important in gene activation and silencing. We generated and characterized a panel of mouse monoclonal antibodies that specifi...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.07035
更新日期:2008-01-01 00:00:00
abstract::Contact reaction is the morphological expression of allogeneic recognition by hemocytes in the ascidian, Halocynthia roretzi; namely they undergo an exocytotic burst upon contact with non-self (allogeneic) hemocytes in vitro. We have found that contact reaction is accompanied by a burst of cyanide-insensitive oxygen c...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.20.81
更新日期:1995-02-01 00:00:00
abstract::Thyroid follicles in vivo are embedded in extracellular matrix (ECM). The composing epithelial cells are in close contact with ECM at the basal side. To examine cell-to-ECM interactions, we studied adhesion, proliferation and differentiation of porcine follicle cells monolayer-cultured on type I and IV collagen, fibro...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.20.345
更新日期:1995-10-01 00:00:00
abstract::Stimulation of polymorphonuclear leukocytes (PMN) by tetravalent concanavalin A (alpha-ConA) induces membrane depolarization preceding the onset of superoxide anion (O2-) production. Both divalent and monovalent ConA analogues were studied to evaluate the role of valence. Monovalent ConA (m-ConA) was inactive in stimu...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.11.199
更新日期:1986-06-01 00:00:00
abstract::All trans retinoic acid (ATRA), a differentiation inducer for human myeloid NB4 cells, induced accumulation of lipid droplet as determined by positivity of Nile Red and Oil Red O in this cell line. Granulocyte colony-stimulating factor (G-CSF), although not having detectable effect by itself, exerted the additive effe...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.28.487
更新日期:2003-10-01 00:00:00
abstract::A granulocyte colony-stimulating factor (G-CSF) was highly purified from the serum-free culture medium of RSP -2 X P3 cells. The G-CSF had an apparent molecular weight of 33,000 as determined by high speed gel permeation chromatography, but its molecular weight was decreased to 15,000 by 0.1% sodium dodecyl sulfate. A...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.9.67
更新日期:1984-03-01 00:00:00
abstract::The ETS family of transcription factors is defined by a conserved DNA-binding ETS domain that forms a winged helix-turn-helix structural motif. This family of transcription factors is involved in a diverse array of biological functions including cellular growth and differentiation, as well as organ development. Among ...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
doi:10.1247/csf.26.19
更新日期:2001-02-01 00:00:00
abstract::A protein phosphatase inhibitor, tautomycin induces blebs on the surface of human myeloid leukemia K562 cells within 10 min. In this paper we examined the cytoskeleton of tautomycin-treated cells. In the presence of tautomycin, cells with blebs turned into segmented forms with smooth surfaces after 15 min and into smo...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.18.33
更新日期:1993-02-01 00:00:00
abstract::Recent studies have demonstrated that vesicle transport to cleavage furrow is indispensable for cytokinesis. Some animal and plant cells form distinct structures during cell division known as central spindle and phragmoplast, respectively. Several essential factors involved in the vesicle transport have been isolated ...
journal_title:Cell structure and function
pub_type: 杂志文章,评审
doi:10.1247/csf.26.567
更新日期:2001-12-01 00:00:00
abstract::When spores of Dictyostelium discoideum matured through normal development were stained with rhodamine-phalloidin, rod-shaped fluorescence was found across the nucleus and in the cytoplasm. Electron micrographs of the rod shape showed that it consisted of long and straight fibers 12 nm in diameter. In contrast, a conv...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.19.189
更新日期:1994-08-01 00:00:00
abstract::It was recently reported that intravenous administration of phorbol 12-myristate 13-acetate (PMA) showed a therapeutic effect in myelocytic leukemia patients. However, we previously observed that, in serum-free conditions, polymorphonuclear leucocytes (PMNs) were killed rapidly by exposure to PMA, suggesting the possi...
journal_title:Cell structure and function
pub_type: 杂志文章
doi:10.1247/csf.26.235
更新日期:2001-08-01 00:00:00