Abstract:
:Histone modifications play critical roles in the epigenetic regulation of gene expression and in the maintenance of genome integrity. Acetylation and methylation of histone H3 are particularly important in gene activation and silencing. We generated and characterized a panel of mouse monoclonal antibodies that specifically recognize different modifications on K4, K9, and K27 residues on histone H3. By using these antibodies for chromatin immunoprecipitation and immunoblotting, we analyzed the relationship between different modifications in nearby nucleosomes in human cells. Within a few nucleosome neighbors, trimethyl-K4 was associated with acetyl-K27, rather than with dimethyl-K4 and acetyl-K9, consistent with their co-localization on active promoters. Furthermore, simultaneous immunofluorescence using directly-labeled antibodies revealed that di- and tri-methylation on K4 was diminished during replicative senescence. These highly-reliable and fully-characterized monoclonal antibodies may facilitate future epigenomic studies on healthy and diseased cells.
journal_name
Cell Struct Functjournal_title
Cell structure and functionauthors
Kimura H,Hayashi-Takanaka Y,Goto Y,Takizawa N,Nozaki Ndoi
10.1247/csf.07035subject
Has Abstractpub_date
2008-01-01 00:00:00pages
61-73issue
1eissn
0386-7196issn
1347-3700pii
JST.JSTAGE/csf/07035journal_volume
33pub_type
杂志文章abstract::Two methods of qualitative analysis of sequence distribution in DNA and protein are presented. The first method is based on the finding that the frequency of occurrence of each nucleotide in a defined sequence with functional significance more or less deviates from uniform distribution. The deviation found in this def...
journal_title:Cell structure and function
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journal_title:Cell structure and function
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journal_title:Cell structure and function
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pub_type: 杂志文章
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