Effects of various types of extracellular matrices on adhesion, proliferation, differentiation, and c-fos protein expression of porcine thyroid follicle cells.


:Thyroid follicles in vivo are embedded in extracellular matrix (ECM). The composing epithelial cells are in close contact with ECM at the basal side. To examine cell-to-ECM interactions, we studied adhesion, proliferation and differentiation of porcine follicle cells monolayer-cultured on type I and IV collagen, fibronectin or laminin. At 3 h in culture, laminin had the lowest rate of cell adhesion. In proliferation, type IV collagen induced the highest level of nuclear bromodeoxyuridine intake. In a functional differentiation, laminin had about 3 times as much triiodothyronine production as the other ECM molecules. In confluent culture cells, we also examined an expression of c-fos protein, a transcription factor that plays crucial roles in signal transduction. Immunocytochemistry detected the protein mainly in the nuclei. Western blot showed that laminin induced the highest level of its expression. Thyrotropin (TSH, 10 mU/ml) did not affect adhesion of the cells on any of the substrata or proliferation of the cells on fibronectin; nor did TSH affect c-fos protein expression of the cells on the substrata except for fibronectin. Our results suggest that type IV collagen and laminin, major components of basement membrane, play positive roles in proliferation and differentiation of follicle cells, respectively, while laminin has no positive effect on adhesion of the cells at early culture; that the cells express c-fos protein even in contact inhibition of growth and its expression is regulated in part by ECM; and that ECM controls some behaviors of the cells in a TSH-dependent or TSH-independent manner.


Cell Struct Funct


Toda S,Matsumura S,Yonemitsu N,Fujitani N,Terayama K,Funatsumaru S,Sugihara H




Has Abstract


1995-10-01 00:00:00












  • Immunohistochemical localization of estradiol, progesterone, and progesterone receptor in human salivary glands and salivary adenoid cystic carcinomas.

    abstract::Immunohistochemical analyses of estradiol, progesterone and progesterone receptor were carried out in human salivary gland and salivary adenoid cystic carcinoma. Immunoreactivity to estradiol and progesterone was found in cytoplasm of the cells of the excretory duct system within normal salivary glands, whereas the pr...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Ozono S,Onozuka M,Sato K,Ito Y

    更新日期:1992-06-01 00:00:00

  • Dual-fluorescence flow cytometric analysis of membrane potential and cytoplasmic free Ca2+ concentration in embryonic rat hippocampal cells.

    abstract::We have demonstrated simultaneous measurement of the membrane potential and cytoplasmic free Ca2+ concentration ([Ca2+]i) by utilizing a dual-laser flow cytometer in embryonic rat hippocampal cell suspensions. Veratrine, a Na+ channel activator, induced both membrane depolarization and elevation of [Ca2+]i. These acti...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Mitsumoto Y,Mohri T

    更新日期:1989-12-01 00:00:00

  • Involvement of prostaglandin-producing pathway in the cytotoxic action of tumor necrosis factor.

    abstract::To elucidate the cytotoxic mechanism of tumor necrosis factor (TNF), we isolated TNF-resistant sublines of L929 cells. As compared with L929 cells, TNF-resistant cells retained similar number and affinity of TNF-binding sites, and showed a similar growth rate. TNF stimulated arachidonate release from L929 cells, while...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Hayakawa M,Oku N,Takagi T,Hori T,Shibamoto S,Yamanaka Y,Takeuchi K,Tsujimoto M,Ito F

    更新日期:1991-08-01 00:00:00

  • Distribution of peanut agglutinin binding sites in rat lymphatic organs.

    abstract::Distribution of peanut agglutinin binding sites was studied histologically with horseradish peroxidase labelled and fluorescein isothiocyanate labelled peanut agglutinin in terms of cell differentiation in rat lymphatic organs, (thymus, spleen, lymph nodes). In this study, alcohol-fixed paraffin-embedded tissue sectio...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Kabir A,Watanabe M,Takeda Z,Kizaki T,Urano Y

    更新日期:1983-03-01 00:00:00

  • The relation between length of the cell cycle duration and RNA content in HeLa S3 cells.

    abstract::The cell cycle of HeLa S3 cells synchronized by hydroxyurea was investigated by flow cytometry. Metachromatic fluorochrome acridine orange was used to strain the DNA and RNA of the cells differentially. Periodic changes in the cellular DNA and RNA contents were observed through five cell cycles. The G1 and S phases of...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Fujikawa-Yamamoto K

    更新日期:1983-09-01 00:00:00

  • Nonlethal G0-ts mutant tsJT60 becomes lethal at the nonpermissive temperature after transformation: a hint for new cancer chemotherapeutics.

    abstract::tsJT60 is a nonlethal temperature-sensitive (ts) mutant of a Fischer rat cell line (3Y1) classified as a G0 mutant; i.e., the ts defect is not expressed within the cell growth cycle but is expressed only between the G0 and S phase. tsJT60 clones transformed with oncogenes such as adenovirus E1A, polyoma large T, polyo...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Tai YY,Ninomiya-Tsuji J,Furuoku K,Ogawa N,Ishibashi S,Shiroki K,Segawa K,Tsuchida N,Shibuya M,Ide T

    更新日期:1990-12-01 00:00:00

  • Isolation of desmosomes from the epidermis of Xenopus laevis and immunochemical characterization of the Xenopus desmosomal cadherins.

    abstract::Here we report a new method of isolating epidermal desmosomes from Xenopus laevis, and a major constituent of desmosomes designated as Xenopus desmogleins (XDsg). Isolation of desmosomes from Xenopus laevis epidermis was carried out by a two step-incubation with different concentrations of NP-40. After discontinuous s...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Ohga R,Shida M,Shida H

    更新日期:2004-02-01 00:00:00

  • Further characterization of the cation channel of a yeast vacuolar membrane in a planar lipid bilayer.

    abstract::A voltage-dependent and Ca2(+)-activated cation channel recently found in the vacuolar membrane of the yeast Saccharomyces cerevisiae was incorporated into planar lipid bilayers and further characterized in macroscopic and single channel levels. Single channel conductances for various cations were in the order: NH4+ g...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Sato M,Tanifuji M,Kasai M

    更新日期:1989-12-01 00:00:00

  • Binding of recombinant human cytokeratin 19 to laminin: a possible role in interaction between intermediate filament derived from epithelial cells and extracellular matrixes.

    abstract::Cytokeratin 8 (CK8) and cytokeratin 19 (CK19) is a specific cytoskeletal component of simple epithelia, including bronchial epithelial cells. We hypothesized that CK8 or CK19 released from epithelial cells may bind to and cause damage to extracellular matrixes through binding of anti-CK8 or anti-CK19 autoantibodies. I...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Dobashi N,Fujita J,Murota M,Ohtsuki Y,Bandoh S,Ueda Y,Dohmoto K,Hojo S,Nishioka M,Ishida T,Takahara J

    更新日期:2000-06-01 00:00:00

  • Intracellular localization and partial amino acid sequence of a stress-inducible 40-kDa protein in HeLa cells.

    abstract::We earlier discovered a novel 40-kDa protein (hsp40) induced by heat shock and other stresses in mammalian and avian cells. In this report, we purified the hsp40 in HeLa cells, using modified two-dimensional gel electrophoresis, and determined the amino terminal amino acid sequence of this protein. The hsp40 is homolo...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Hattori H,Liu YC,Tohnai I,Ueda M,Kaneda T,Kobayashi T,Tanabe K,Ohtsuka K

    更新日期:1992-02-01 00:00:00

  • Heavy ion microbeam irradiation induces ultrastructural changes in isolated single fibers of skeletal muscle.

    abstract::The effects of heavy ion microbeams on muscle fibers isolated from mouse skeletal muscles were examined by electron microscopy. The plasma membranes of heavy ion beam-irradiated areas of muscle fibers showed irregular protrusions and invaginations. In the cytoplasm, an irregular distribution of microfilaments was foun...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Hino M,Wada S,Tajika Y,Morimura Y,Hamada N,Funayama T,Sakashita T,Kakizaki T,Kobayashi Y,Yorifuji H

    更新日期:2007-01-01 00:00:00

  • Regulatory role of extracellular matrix components in expression of matrix metalloproteinases in cultured hepatic stellate cells.

    abstract::Hepatic stellate cells (HSCs) were changed in their morphology, proliferative activity, and functions by culturing on type I collagen gel, as compared to the culture on polystyrene surface. HSCs have been found to produce extracellular matrix components and matrix metalloproteinases (MMPs). In this study, we have asse...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Li YL,Sato M,Kojima N,Miura M,Senoo H

    更新日期:1999-10-01 00:00:00

  • Hepatic stellate cells: unique characteristics in cell biology and phenotype.

    abstract::Hepatic stellate cells (HSCs), a mesenchymal cell type in hepatic parenchyma, have unique features with respect to their cellular origin, morphology, and function. Normal, quiescent HSCs function as major vitamin A-storing cells containing over 80% of total vitamin A in the body to maintain vitamin A homeostasis. HSCs...

    journal_title:Cell structure and function

    pub_type: 杂志文章,评审


    authors: Sato M,Suzuki S,Senoo H

    更新日期:2003-04-01 00:00:00

  • Prediction of the virulencies of some enveloped viruses from the structure of the cleavage recognition site of viral glycoprotein essential for infectivity. II. Deviation analysis.

    abstract::Analysis of the amino acid sequence in protein (deviation analysis) suggests that the binding between trypsin and the enveloped virus is the first step of their interaction, which occurs in a specified configuration. It is possible that the distance between their active sites is important for the viral sensitivity to ...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Kiho Y,Miyata K,Okada Y

    更新日期:1989-12-01 00:00:00

  • Light-independent and dependent phases of proplastid development in Euglena gracilis W3BUL.

    abstract::Cells of Euglena gracilis Klebs var. bacillaris Cori mutant W3BUL grown in darkness on Hutner's pH 3.5 medium without agitation accumulate wax ester. These cells have undeveloped proplastid remnants characteristic of this mutant. If these cells are transferred to an inorganic medium and bubbled with 2-3% CO2 in air, t...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Osafune T,Schiff JA,Hase E

    更新日期:1987-10-01 00:00:00

  • Enhancement of diphtheria toxin-induced apoptosis in Vero cells by combination treatment with brefeldin A and okadaic acid.

    abstract::In the present study, we compared the abilities of ricin and diphtheria toxin to induce apoptosis in Vero cells. The cytolysis and DNA fragmentation by ricin paralleled its protein synthesis inhibitory activity. However, unlike ricin, diphtheria toxin could induce neither cytolysis nor DNA fragmentation in Vero cells ...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Kusano I,Kageyama A,Tamura T,Oda T,Muramatsu T

    更新日期:2001-10-01 00:00:00

  • Phosphorylation of RanGAP1 stabilizes its interaction with Ran and RanBP1.

    abstract::Ran is a nuclear Ras-like GTPase that is required for various nuclear events including the bi-directional transport of proteins and ribonucleoproteins through the nuclear pore complex, spindle formation, and reassembly of the nuclear envelope. One of the key regulators of Ran is RanGAP1, a Ran specific GTPase activati...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Takeda E,Hieda M,Katahira J,Yoneda Y

    更新日期:2005-01-01 00:00:00

  • The molecular mechanism of targeted vesicle transport in cytokinesis.

    abstract::Recent studies have demonstrated that vesicle transport to cleavage furrow is indispensable for cytokinesis. Some animal and plant cells form distinct structures during cell division known as central spindle and phragmoplast, respectively. Several essential factors involved in the vesicle transport have been isolated ...

    journal_title:Cell structure and function

    pub_type: 杂志文章,评审


    authors: Edamatsu M

    更新日期:2001-12-01 00:00:00

  • Effects of surface substances of untransformed fibroblastic cells on the adhesion and proliferation of neighboring cells: a study using fixed confluent cell sheets.

    abstract::To study the effects of surface materials of cells on the behavior of other neighboring cells in a crowded culture, confluent sheets of rat 3Y1 fibroblasts were fixed and then 3Y1 cells were seeded on to them. Among confluent sheets unfixed, fixed with formalin and fixed with ethanol and an empty plastic dish surface,...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Okuda A,Sasaki M,Kimura G

    更新日期:1990-10-01 00:00:00

  • Further investigation of some inhibitors on myogenic differentiation: mechanism of inhibition with HMBA on quail myoblasts transformed with Rous sarcoma virus.

    abstract::To investigate the mechanism of myogenic differentiation, we are using quail myoblast cells (QM cells) transformed with a temperature-sensitive mutant of Rous sarcoma virus (ts-RSV), termed QM-RSV cells. At 35.5 degrees C, a permissive temperature for RSV, QM-RSV cells repeatedly proliferate without differentiation, b...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Hase H,Hirayama E,Kim J

    更新日期:1996-02-01 00:00:00

  • Soluble sperm extract triggers inositol 1,4,5-trisphosphate-induced Ca2+ release in the oocytes of the sea urchin, Anthocidaris crassispina.

    abstract::The substance in sperm which induces the activation of eggs at fertilization has yet to be elucidated. Osawa et al. reported that the soluble extract of sperm (spex) causes inositol 1,4,5-trisphosphate (IP3)-induced Ca2+ release (IICR) in fertilized eggs of sea urchins when externally applied [Osawa et al., (1992). Zo...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Osawa M

    更新日期:1994-04-01 00:00:00

  • Microinjection of the monoclonal anti-tubulin antibody YL1/2 inhibits cleavage of sand dollar eggs.

    abstract::Two monoclonal antibodies against alpha-tubulin (YL1/2 and D2D6) were microinjected into the egg of the sand dollar Clypeaster japonicus, and their effects on cleavage of the egg were investigated. They had already been shown by immunoblotting to react specifically with egg tubulin and by immunofluorescence to stain t...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Oka MT,Arai T,Hamaguchi Y

    更新日期:1990-12-01 00:00:00

  • Expression of a 66-kD heat shock protein associated with the process of cyst formation of a true slime mold, Physarum polycephalum.

    abstract::Under unfavorable conditions for growth, haploid myxoamoebae of Physarum polycephalum retracted their pseudopodia and changed their cell shape into disk-like form, after which they constructed the cell walls to form microcysts. These morphological changes of haploid cells were associated with changes in intracellular ...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Shimada Y,Kasakura T,Yokota M,Miyata Y,Murofushi H,Sakai H,Yahara I,Murakami-Murofushi K

    更新日期:1992-10-01 00:00:00

  • Loosening of a preprophase band of microtubules in onion (Allium cepa L.) root tip cells by kinase inhibitors.

    abstract::Effects of kinase inhibitors on the preprophase band of microtubules in onion (Allium cepa L.) root tip cells were examined. Bundled microtubules in preprophase bands were dispersed on the cell cortex when onion seedlings were incubated with 2.5-5.0 mM 6-dimethylaminopurine. Fifteen min was enough for the bundled micr...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Nogami A,Mineyuki Y

    更新日期:1999-10-01 00:00:00

  • MiR-187-3p Enhances Propranolol Sensitivity of Hemangioma Stem Cells.

    abstract::Infantile hemangioma is the most common soft tissue tumors in childhood. In clinic, propranolol is widely used for infantile hemangioma therapy. However, some of the infantile hemangioma patients display resistance to propranolol treatment. Previous studies show that miR-187-3p is inhibited in hepatocellular carcinoma...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Liu C,Zhao Z,Ji Z,Jiang Y,Zheng J

    更新日期:2019-03-20 00:00:00

  • Sperm morphology and IMP distribution in membranes of spermatozoa of cyprinid fishes II.

    abstract::The morphology and intramembranous particle (IMP) distribution of spermatozoa were investigated in four species of cyprinid fishes, Gnathopogon elongatus elongatus, Pseudogobio esocinus, Acheilognathus lanceolatus and Puntius tetrazona. In particular, observation of IMP distribution was conducted as an aspect of compa...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Ohta T,Matsuda M

    更新日期:1995-08-01 00:00:00

  • Chromatin structure with respect to histone signature changes during cell differentiation.

    abstract::Here, we would like to point out important milestones in the study of nuclear radial positioning and gene expression during differentiation processes. In addition, changes in the histone signature that significantly precede various differentiation pathways are reviewed. We address the regulatory functions of chromatin...

    journal_title:Cell structure and function

    pub_type: 杂志文章,评审


    authors: Harnicarová Horáková A,Bártová E,Kozubek S

    更新日期:2010-01-01 00:00:00

  • The effect of cytokines on the differentiation of an eosinophilic leukemia cell line (EoL-1) is associated with down regulation of c-myc gene expression.

    abstract::In this study, we measured hydrogen peroxide (H2O2) release as one of the functions of mature eosinophils, and utilized it as a quantitative index. We demonstrated that 1) the human eosinophilic leukemia cell line, EoL-1, did not release H2O2 when stimulated with phorbol myristate acetate (PMA), but after culturing wi...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Ohtsu H,Yamauchi K,Yoshie O,Tanno Y,Saito H,Hayashi N,Takishima T

    更新日期:1993-04-01 00:00:00

  • Inhibition of cell proliferation by a unique lysophosphatidic acid, PHYLPA, isolated from Physarum polycephalum: signaling events of antiproliferative action by PHYLPA.

    abstract::The unique Physarum lysophosphatidic acid, PHYLPA, having a cyclopropane in the fatty acid moiety and a cyclic phosphate at C-2 and C-3 positions of the glycerol, inhibited proliferation of human fibroblast cells, TIG-3 and TIG-7, which were cultured in a chemically defined (serum-free) medium. The cells at S- and M-p...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Murakami-Murofushi K,Kaji K,Kano K,Fukuda M,Shioda M,Murofushi H

    更新日期:1993-10-01 00:00:00

  • Transcriptional activation of mouse major satellite regions during neuronal differentiation.

    abstract::Recent studies have revealed various biological functions for repetitive sequences, which make up about half of the human genome. One such sequence, major satellites, which are tandem repetitive sequences adjacent to the centromere, have been shown to be a kinetochore component that plays a role in the formation and f...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Kishi Y,Kondo S,Gotoh Y

    更新日期:2012-01-01 00:00:00