Effects of various types of extracellular matrices on adhesion, proliferation, differentiation, and c-fos protein expression of porcine thyroid follicle cells.


:Thyroid follicles in vivo are embedded in extracellular matrix (ECM). The composing epithelial cells are in close contact with ECM at the basal side. To examine cell-to-ECM interactions, we studied adhesion, proliferation and differentiation of porcine follicle cells monolayer-cultured on type I and IV collagen, fibronectin or laminin. At 3 h in culture, laminin had the lowest rate of cell adhesion. In proliferation, type IV collagen induced the highest level of nuclear bromodeoxyuridine intake. In a functional differentiation, laminin had about 3 times as much triiodothyronine production as the other ECM molecules. In confluent culture cells, we also examined an expression of c-fos protein, a transcription factor that plays crucial roles in signal transduction. Immunocytochemistry detected the protein mainly in the nuclei. Western blot showed that laminin induced the highest level of its expression. Thyrotropin (TSH, 10 mU/ml) did not affect adhesion of the cells on any of the substrata or proliferation of the cells on fibronectin; nor did TSH affect c-fos protein expression of the cells on the substrata except for fibronectin. Our results suggest that type IV collagen and laminin, major components of basement membrane, play positive roles in proliferation and differentiation of follicle cells, respectively, while laminin has no positive effect on adhesion of the cells at early culture; that the cells express c-fos protein even in contact inhibition of growth and its expression is regulated in part by ECM; and that ECM controls some behaviors of the cells in a TSH-dependent or TSH-independent manner.


Cell Struct Funct


Toda S,Matsumura S,Yonemitsu N,Fujitani N,Terayama K,Funatsumaru S,Sugihara H




Has Abstract


1995-10-01 00:00:00












  • Elevation of transforming growth factor beta 1 mRNA level in ES-D3 mouse embryonic stem cells cocultured with Balb/c3T3 A31 fibroblasts.

    abstract::Embryonic stem (ES) cells are a pluripotent cell line derived from the inner cell mass of the mouse blastocyst. The mRNA levels of several growth-related genes were examined in ES cells by Northern blot analysis under several different growth conditions. In a coculture of ES cells with the mouse fibroblast cell line, ...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Katsube K,Shimizu N

    更新日期:1991-10-01 00:00:00

  • Binding of recombinant human cytokeratin 19 to laminin: a possible role in interaction between intermediate filament derived from epithelial cells and extracellular matrixes.

    abstract::Cytokeratin 8 (CK8) and cytokeratin 19 (CK19) is a specific cytoskeletal component of simple epithelia, including bronchial epithelial cells. We hypothesized that CK8 or CK19 released from epithelial cells may bind to and cause damage to extracellular matrixes through binding of anti-CK8 or anti-CK19 autoantibodies. I...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Dobashi N,Fujita J,Murota M,Ohtsuki Y,Bandoh S,Ueda Y,Dohmoto K,Hojo S,Nishioka M,Ishida T,Takahara J

    更新日期:2000-06-01 00:00:00

  • Soluble megalin is accumulated in the lumen of the rat endolymphatic sac.

    abstract::The endolymphatic sac (ES) is believed to play an important role in maintaining homeostasis in the inner ear by the absorption and endocytosis of endolymph. Megalin is a 600-kDa multiligand endocytic receptor expressed in certain types of absorptive epithelia including kidney proximal tubules. We analyzed the immunore...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Ishida T,Hatae T,Nishi N,Araki N

    更新日期:2006-01-01 00:00:00

  • The early secretory pathway contributes to autophagy in yeast.

    abstract::Autophagy is a starvation response in eukaryotes by which the cell delivers cytoplasmic components to the vacuole for degradation, and is mediated by a double membrane structure called the autophagosome. We have previously proposed that the specific combination of COPII like components, including Sec24p, is required f...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Hamasaki M,Noda T,Ohsumi Y

    更新日期:2003-02-01 00:00:00

  • Heavy ion microbeam irradiation induces ultrastructural changes in isolated single fibers of skeletal muscle.

    abstract::The effects of heavy ion microbeams on muscle fibers isolated from mouse skeletal muscles were examined by electron microscopy. The plasma membranes of heavy ion beam-irradiated areas of muscle fibers showed irregular protrusions and invaginations. In the cytoplasm, an irregular distribution of microfilaments was foun...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Hino M,Wada S,Tajika Y,Morimura Y,Hamada N,Funayama T,Sakashita T,Kakizaki T,Kobayashi Y,Yorifuji H

    更新日期:2007-01-01 00:00:00

  • Structural interactions responsible for the assembly of the troponin complex on the muscle thin filament.

    abstract::Skeletal muscle contraction is regulated by a complex of five polypeptides which are stably associated with the actin filament. This complex consists of two proteins: troponin with three subunits (TnC; TnI and TnT) and tropomyosin (a dimer of two chains). Using deletion mutants of TnC, TnI and TnT we determined that e...

    journal_title:Cell structure and function

    pub_type: 杂志文章,评审


    authors: Reinach FC,Farah CS,Monteiro PB,Malnic B

    更新日期:1997-02-01 00:00:00

  • Organization of protein and mRNA for titin and other myofibril components during myofibrillogenesis in cultured chicken skeletal muscle.

    abstract::Myofibril assembly requires the cell to join diverse components, correctly oriented to the rest of the cell. Titin, a huge elastic protein with a role in myogenesis, assembles during translation in vivo and may require spatially organized mRNA to allow assembly. By immunofluorescence, we examined titin and myosin prot...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Fulton AB,Alftine C

    更新日期:1997-02-01 00:00:00

  • Caffeine alone causes DNA damage in Chinese hamster ovary cells.

    abstract::Caffeine has been shown to enhance the lethal effect of DNA-damaging agents in mammalian cells, and the potentiation by caffeine of this effect is generally interpreted as the result of inhibition by caffeine of the repair of damaged DNA. However, the mechanism by which caffeine enhances the lethal effect of DNA-damag...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Ishida R,Kozaki M,Takahashi T

    更新日期:1985-12-01 00:00:00

  • Distribution of DNA polymerase alpha during nuclear division cycles in Drosophila melanogaster embryo.

    abstract::An immunocytochemical method using a specific monoclonal antibody was employed to detect DNA polymerase alpha in Drosophila melanogaster embryos during the first 13 nuclear division cycles after fertilization. The anti-DNA polymerase alpha antibody stained the ooplasm of the unfertilized egg, indicating that DNA polym...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Yamaguchi M,Kuroda K,Hirose F,Matsukage A

    更新日期:1992-04-01 00:00:00

  • Novel actin rods appeared in spores of Dictyostelium discoideum.

    abstract::When spores of Dictyostelium discoideum matured through normal development were stained with rhodamine-phalloidin, rod-shaped fluorescence was found across the nucleus and in the cytoplasm. Electron micrographs of the rod shape showed that it consisted of long and straight fibers 12 nm in diameter. In contrast, a conv...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Sameshima M,Chijiiwa Y,Kishi Y,Hashimoto Y

    更新日期:1994-08-01 00:00:00

  • The effect of cytokines on the differentiation of an eosinophilic leukemia cell line (EoL-1) is associated with down regulation of c-myc gene expression.

    abstract::In this study, we measured hydrogen peroxide (H2O2) release as one of the functions of mature eosinophils, and utilized it as a quantitative index. We demonstrated that 1) the human eosinophilic leukemia cell line, EoL-1, did not release H2O2 when stimulated with phorbol myristate acetate (PMA), but after culturing wi...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Ohtsu H,Yamauchi K,Yoshie O,Tanno Y,Saito H,Hayashi N,Takishima T

    更新日期:1993-04-01 00:00:00

  • Hepatic stellate cells: unique characteristics in cell biology and phenotype.

    abstract::Hepatic stellate cells (HSCs), a mesenchymal cell type in hepatic parenchyma, have unique features with respect to their cellular origin, morphology, and function. Normal, quiescent HSCs function as major vitamin A-storing cells containing over 80% of total vitamin A in the body to maintain vitamin A homeostasis. HSCs...

    journal_title:Cell structure and function

    pub_type: 杂志文章,评审


    authors: Sato M,Suzuki S,Senoo H

    更新日期:2003-04-01 00:00:00

  • Expression of a 66-kD heat shock protein associated with the process of cyst formation of a true slime mold, Physarum polycephalum.

    abstract::Under unfavorable conditions for growth, haploid myxoamoebae of Physarum polycephalum retracted their pseudopodia and changed their cell shape into disk-like form, after which they constructed the cell walls to form microcysts. These morphological changes of haploid cells were associated with changes in intracellular ...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Shimada Y,Kasakura T,Yokota M,Miyata Y,Murofushi H,Sakai H,Yahara I,Murakami-Murofushi K

    更新日期:1992-10-01 00:00:00

  • Loosening of a preprophase band of microtubules in onion (Allium cepa L.) root tip cells by kinase inhibitors.

    abstract::Effects of kinase inhibitors on the preprophase band of microtubules in onion (Allium cepa L.) root tip cells were examined. Bundled microtubules in preprophase bands were dispersed on the cell cortex when onion seedlings were incubated with 2.5-5.0 mM 6-dimethylaminopurine. Fifteen min was enough for the bundled micr...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Nogami A,Mineyuki Y

    更新日期:1999-10-01 00:00:00

  • Developmental changes of synapsin I subcellular localization in rat cerebellar neurons.

    abstract::Synapsin I, one of the major synaptic proteins, is thought to associate with synaptic vesicles and to play a regulatory role in neurotransmitter release. In mature neurons, it is concentrated almost exclusively in presynaptic nerve endings. Here, we studied the subcellular localization of synapsin I during the develop...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Harada A,Sobue K,Hirokawa N

    更新日期:1990-12-01 00:00:00

  • Structural changes at the site of Tritrichomonas foetus-erythrocyte interaction.

    abstract::Tritrichomonas foetus strongly agglutinates human erythrocytes, thus suggesting the occurrence of an adhesin associated with its surface. Adherence was observed immediately after mixing of the parasites with erythrocytes and the intensity increased for up to 30 min. Scanning electron microscopy examination of T. foetu...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: da Silva NS,Dias Filho BP,De Souza W

    更新日期:1996-08-01 00:00:00

  • Down-regulated RPS3a/nbl expression during retinoid-induced differentiation of HL-60 cells: a close association with diminished susceptibility to actinomycin D-stimulated apoptosis.

    abstract::The efficacy of anticancer agents significantly depends on the differential susceptibility of undifferentiated cancer cells and differentiated normal cells to undergo apoptosis. We previously found that enhanced expression of RPS3a/nbl, which apparently encodes a ribosomal protein, seems to prime cells for apoptosis, ...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Russell L,Naora H,Naora H

    更新日期:2000-04-01 00:00:00

  • High voltage electric pulses efficiently induce fusion of cells in monolayer culture.

    abstract::High voltage electric pulses, 1000 V/cm, were found to induce cell fusion efficiently when delivered to cells growing in a monolayer culture. The maximum yield of fused cells was about 30% of cells remaining after treatment. Colonies of interspecies hybrid cells between mouse and others also appeared with a frequency ...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Ishizaki K,Chang HR,Eguchi T,Ikenaga M

    更新日期:1989-04-01 00:00:00

  • Isolation and characterization of nuclei from rice embryos.

    abstract::A method has been developed to isolate pure preparations of nuclei in high yield from commercially available viable rice embryos (germ), employing extraction with buffer solution containing glycerol (without detergent) and polyamine, followed by centrifugation on a 30% Percoll cushion. The intactness of the isolated n...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Yamaguchi J,Lim PY,Aratani K,Akazawa T

    更新日期:1992-04-01 00:00:00

  • MiR-187-3p Enhances Propranolol Sensitivity of Hemangioma Stem Cells.

    abstract::Infantile hemangioma is the most common soft tissue tumors in childhood. In clinic, propranolol is widely used for infantile hemangioma therapy. However, some of the infantile hemangioma patients display resistance to propranolol treatment. Previous studies show that miR-187-3p is inhibited in hepatocellular carcinoma...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Liu C,Zhao Z,Ji Z,Jiang Y,Zheng J

    更新日期:2019-03-20 00:00:00

  • Role of ETS family transcription factors in vascular development and angiogenesis.

    abstract::The ETS family of transcription factors is defined by a conserved DNA-binding ETS domain that forms a winged helix-turn-helix structural motif. This family of transcription factors is involved in a diverse array of biological functions including cellular growth and differentiation, as well as organ development. Among ...

    journal_title:Cell structure and function

    pub_type: 杂志文章,评审


    authors: Sato Y

    更新日期:2001-02-01 00:00:00

  • Immunohistochemical localization of estradiol, progesterone, and progesterone receptor in human salivary glands and salivary adenoid cystic carcinomas.

    abstract::Immunohistochemical analyses of estradiol, progesterone and progesterone receptor were carried out in human salivary gland and salivary adenoid cystic carcinoma. Immunoreactivity to estradiol and progesterone was found in cytoplasm of the cells of the excretory duct system within normal salivary glands, whereas the pr...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Ozono S,Onozuka M,Sato K,Ito Y

    更新日期:1992-06-01 00:00:00

  • Lipid droplet formation in human myeloid NB4 cells stimulated by all trans retinoic acid and granulocyte colony-stimulating factor: possible involvement of peroxisome proliferator-activated receptor gamma.

    abstract::All trans retinoic acid (ATRA), a differentiation inducer for human myeloid NB4 cells, induced accumulation of lipid droplet as determined by positivity of Nile Red and Oil Red O in this cell line. Granulocyte colony-stimulating factor (G-CSF), although not having detectable effect by itself, exerted the additive effe...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Inazawa Y,Nakatsu M,Yasugi E,Saeki K,Yuo A

    更新日期:2003-10-01 00:00:00

  • Retinobenzoic acids and nuclear retinoic acid receptors.

    abstract::Retinoids (retinoic acid and its analogs) are widely involved in the control of cell proliferation, cell differentiation and embryonic development. A series of potent and novel synthetic retinoids named retinobenzoic acids has been developed. Retinobenzoic acids have proven to be useful tools in the investigation of t...

    journal_title:Cell structure and function

    pub_type: 杂志文章,评审


    authors: Hashimoto Y

    更新日期:1991-04-01 00:00:00

  • Domain analysis of the tetraspanins: studies of CD9/CD63 chimeric molecules on subcellular localization and upregulation activity for diphtheria toxin binding.

    abstract::CD9 and CD63 belong to a tetramembrane-spanning glycoprotein family called tetraspanin, and are involved in a wide variety of cellular processes, but the structure-function relationship of this family of proteins has yet to be clarified. CD9 associates with diphtheria toxin receptor (DTR), which is identical to the me...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Ryu F,Takahashi T,Nakamura K,Takahashi Y,Kobayashi T,Shida S,Kameyama T,Mekada E

    更新日期:2000-10-01 00:00:00

  • Neuronal differentiation of mouse neural crest cells in vitro.

    abstract::The purpose of the present study is to analyze the effect of serum or chick embryo extract (CEE) on the neuronal differentiation of the mouse neural crest cells. When the crest cells were cultured in the medium containing serum at low concentration (5% calf serum), neurite outgrowth was observed. The active outgrowth ...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Ito K,Morita T,Takeuchi T

    更新日期:1988-06-01 00:00:00

  • The organization of histone H3 modifications as revealed by a panel of specific monoclonal antibodies.

    abstract::Histone modifications play critical roles in the epigenetic regulation of gene expression and in the maintenance of genome integrity. Acetylation and methylation of histone H3 are particularly important in gene activation and silencing. We generated and characterized a panel of mouse monoclonal antibodies that specifi...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Kimura H,Hayashi-Takanaka Y,Goto Y,Takizawa N,Nozaki N

    更新日期:2008-01-01 00:00:00

  • Molecular mechanisms of myofibril assembly in heart.

    abstract::We investigated the assembly of the first sarcomeres in chicken embryos by confocal microscopy of immunofluorescently stained whole mount rudiments of early chicken hearts isolated around the onset of beating. In embryos with merely 9 somites, myomesin was found to be present in a cross striated pattern, indicating th...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Auerbach D,Rothen-Ruthishauser B,Bantle S,Leu M,Ehler E,Helfman D,Perriard JC

    更新日期:1997-02-01 00:00:00

  • Soluble sperm extract triggers inositol 1,4,5-trisphosphate-induced Ca2+ release in the oocytes of the sea urchin, Anthocidaris crassispina.

    abstract::The substance in sperm which induces the activation of eggs at fertilization has yet to be elucidated. Osawa et al. reported that the soluble extract of sperm (spex) causes inositol 1,4,5-trisphosphate (IP3)-induced Ca2+ release (IICR) in fertilized eggs of sea urchins when externally applied [Osawa et al., (1992). Zo...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Osawa M

    更新日期:1994-04-01 00:00:00

  • Microtuble organization in Xenopus eggs during the first cleavage and its role in cytokinesis.

    abstract::It has been suggested that the organization of microtubules during mitosis plays an important role in cytokinesis in animal cells. We studied the organization of microtubules during the first cleavage and its role in cytokinesis of Xenopus eggs. First, we examined the immunofluorescent localization of microtubules in ...

    journal_title:Cell structure and function

    pub_type: 杂志文章


    authors: Takayama M,Noguchi T,Yamashiro S,Mabuchi I

    更新日期:2002-08-01 00:00:00