High voltage electric pulses efficiently induce fusion of cells in monolayer culture.

abstract：:The relative fibrin-binding, fibrinolytic and fibrinogenolytic properties of single-chain pro-urokinase, an inactive proenzyme form of human urokinase purified from cultured human kidney cells, and urokinase were compared. The affinity of single-chain pro-urokinase for fibrin was much higher than that of urokinase. In...

journal_title：Cell structure and function

authors： Kasai S,Arimura H,Nishida M,Suyama T

更新日期：1985-06-01 00:00:00

abstract：:The Acanthamoeba myosin-IA and myosin-IB molecular motors bind to membranes, so they may produce the force to move organelles and membranes along actin filaments. We have determined the rate constants for the actin-activated myosin-I ATPase by pre-steady state kinetic analysis. ATP binds rapidly to myosin-I and dissoc...

journal_title：Cell structure and function

authors： Pollard TD,Ostap EM

更新日期：1996-10-01 00:00:00

abstract：:Thyroid follicles in vivo are embedded in extracellular matrix (ECM). The composing epithelial cells are in close contact with ECM at the basal side. To examine cell-to-ECM interactions, we studied adhesion, proliferation and differentiation of porcine follicle cells monolayer-cultured on type I and IV collagen, fibro...

journal_title：Cell structure and function

authors： Toda S,Matsumura S,Yonemitsu N,Fujitani N,Terayama K,Funatsumaru S,Sugihara H

更新日期：1995-10-01 00:00:00

abstract：:We investigated the assembly of the first sarcomeres in chicken embryos by confocal microscopy of immunofluorescently stained whole mount rudiments of early chicken hearts isolated around the onset of beating. In embryos with merely 9 somites, myomesin was found to be present in a cross striated pattern, indicating th...

journal_title：Cell structure and function

authors： Auerbach D,Rothen-Ruthishauser B,Bantle S,Leu M,Ehler E,Helfman D,Perriard JC

更新日期：1997-02-01 00:00:00

abstract：:Two-dimensional gel electrophoresis revealed two protein spots, a (molecular weight 70,000, pI 4.6) and b (molecular weight 69,000, pI 4.4) in PC-12 cells (rat pheochromocytoma cells). When the cells were induced to differentiate with nerve growth factor, the amount of protein in spot a, and later spot b increased wit...

journal_title：Cell structure and function

authors： Noguchi S,Muramatsu T,Koike T

更新日期：1987-02-01 00:00:00

abstract：:To study the effects of surface materials of cells on the behavior of other neighboring cells in a crowded culture, confluent sheets of rat 3Y1 fibroblasts were fixed and then 3Y1 cells were seeded on to them. Among confluent sheets unfixed, fixed with formalin and fixed with ethanol and an empty plastic dish surface,...

journal_title：Cell structure and function

authors： Okuda A,Sasaki M,Kimura G

更新日期：1990-10-01 00:00:00

abstract：:A monoclonal type M-immunoglobulin (IgM) was generated in mice against a nuclease-urea extract of HeLa metaphase chromosomes. This antibody stains metaphase chromosomes from a variety of mammalian cultured cell types by indirect immunofluorescence. Antibody 12C7 reacts by western transfer technique with histone H1 in ...

journal_title：Cell structure and function

authors： Ortiz M,Bejarano LA,Rendón MC,Martin G,Brinkley BR,Valdivia MM

更新日期：1991-08-01 00:00:00

abstract：:Ran is a nuclear Ras-like GTPase that is required for various nuclear events including the bi-directional transport of proteins and ribonucleoproteins through the nuclear pore complex, spindle formation, and reassembly of the nuclear envelope. One of the key regulators of Ran is RanGAP1, a Ran specific GTPase activati...

journal_title：Cell structure and function

authors： Takeda E,Hieda M,Katahira J,Yoneda Y

更新日期：2005-01-01 00:00:00

abstract：:The localization of mRNA in neuronal dendrites plays a role in both locally and temporally regulated protein synthesis, which is required for certain forms of synaptic plasticity. RNA granules constitute a dendritic mRNA transport machinery in neurons, which move along microtubules. RNA granules contain densely packed...

journal_title：Cell structure and function

authors： Miki T,Takano K,Yoneda Y

更新日期：2005-01-01 00:00:00

abstract：:To elucidate the cytotoxic mechanism of tumor necrosis factor (TNF), we isolated TNF-resistant sublines of L929 cells. As compared with L929 cells, TNF-resistant cells retained similar number and affinity of TNF-binding sites, and showed a similar growth rate. TNF stimulated arachidonate release from L929 cells, while...

journal_title：Cell structure and function

authors： Hayakawa M,Oku N,Takagi T,Hori T,Shibamoto S,Yamanaka Y,Takeuchi K,Tsujimoto M,Ito F

更新日期：1991-08-01 00:00:00

abstract：:Under unfavorable conditions for growth, haploid myxoamoebae of Physarum polycephalum retracted their pseudopodia and changed their cell shape into disk-like form, after which they constructed the cell walls to form microcysts. These morphological changes of haploid cells were associated with changes in intracellular ...

journal_title：Cell structure and function

authors： Shimada Y,Kasakura T,Yokota M,Miyata Y,Murofushi H,Sakai H,Yahara I,Murakami-Murofushi K

更新日期：1992-10-01 00:00:00

abstract：:Synapsin I, one of the major synaptic proteins, is thought to associate with synaptic vesicles and to play a regulatory role in neurotransmitter release. In mature neurons, it is concentrated almost exclusively in presynaptic nerve endings. Here, we studied the subcellular localization of synapsin I during the develop...

journal_title：Cell structure and function

authors： Harada A,Sobue K,Hirokawa N

更新日期：1990-12-01 00:00:00

abstract：:The cell cycle of HeLa S3 cells synchronized by hydroxyurea was investigated by flow cytometry. Metachromatic fluorochrome acridine orange was used to strain the DNA and RNA of the cells differentially. Periodic changes in the cellular DNA and RNA contents were observed through five cell cycles. The G1 and S phases of...

journal_title：Cell structure and function

authors： Fujikawa-Yamamoto K

更新日期：1983-09-01 00:00:00

abstract：:Retinoids (retinoic acid and its analogs) are widely involved in the control of cell proliferation, cell differentiation and embryonic development. A series of potent and novel synthetic retinoids named retinobenzoic acids has been developed. Retinobenzoic acids have proven to be useful tools in the investigation of t...

journal_title：Cell structure and function

authors： Hashimoto Y

更新日期：1991-04-01 00:00:00

abstract：:Mechanistic target of rapamycin complex 1 (mTORC1) plays a pivotal role in controlling cell growth and metabolism in response to nutrients and growth factors. The activity of mTORC1 is dually regulated by amino acids and growth factor signaling, and amino acid-dependent mTORC1 activity is regulated by mTORC1 interacti...

journal_title：Cell structure and function

authors： Ito S,Nada S,Yamazaki D,Kimura T,Kajiwara K,Miki H,Okada M

更新日期：2020-01-01 00:00:00

abstract：:alpha-1,3-Fucosyltransferase (Fuc T IV) cDNA was placed under the control of beta-actin, cytomegalovirus enhancer/promoter and transfected into embryonic stem cells. The transfected cell clones with integrated cDNA (positive clones) differentiated more efficiently into myocardial cell clones without integrated cDNA (n...

journal_title：Cell structure and function

authors： Sudou A,Muramatsu H,Kaname T,Kadomatsu K,Muramatsu T

更新日期：1997-04-01 00:00:00

abstract：:To construct recombinant retroviruses with only a single active promoter, we introduced point mutations into the TATA box region of the 3'-LTR, and successfully obtained high-titer virus with sufficient self-inactivating activity. However, the viral titer could not be determined by the number of G418 resistant colonie...

journal_title：Cell structure and function

authors： Miyao Y,Shimizu K,Tamura M,Yamada M,Tamura K,Nakahira K,Kuriyama S,Hayakawa T,Ikenaka K

更新日期：1995-04-01 00:00:00

abstract：:A granulocyte colony-stimulating factor (G-CSF) was highly purified from the serum-free culture medium of RSP -2 X P3 cells. The G-CSF had an apparent molecular weight of 33,000 as determined by high speed gel permeation chromatography, but its molecular weight was decreased to 15,000 by 0.1% sodium dodecyl sulfate. A...

journal_title：Cell structure and function

authors： Tsuneoka K,Shikita M

更新日期：1984-03-01 00:00:00

abstract：:Nonmuscle myosin II activity is regulated by phosphorylation of the myosin II regulatory light chain (MRLC) at Ser19 or at both Thr18 and Ser19, and the phosphorylation of MRLC promotes the contractility and stability of actomyosin. To analyze the states of MRLC phosphorylation at the leading edge in the motile HeLa c...

journal_title：Cell structure and function

authors： Uchimura T,Fumoto K,Yamamoto Y,Ueda K,Hosoya H

更新日期：2002-12-01 00:00:00

abstract：:A protein phosphatase inhibitor, tautomycin induces blebs on the surface of human myeloid leukemia K562 cells within 10 min. In this paper we examined the cytoskeleton of tautomycin-treated cells. In the presence of tautomycin, cells with blebs turned into segmented forms with smooth surfaces after 15 min and into smo...

journal_title：Cell structure and function

authors： Kurisaki T,Magae J,Nagai K,Hirata A,Kusaka I,Shinji H,Morikawa M,Yoshida T,Isono K,Yamasaki M

更新日期：1993-02-01 00:00:00

abstract：:Upon tryptic digestion of synaptosomes, ATPase activities decreased in the order of Cl(-)-ATPase greater than or equal to Na+,K+-ATPase greater than anion-insensitive Mg2+-ATPase. Upon synaptosome treatment with hypotonic solution, Cl(-)-ATPase or anion-insensitive Mg2+-ATPase was slightly inactivated, while Na+,K+-AT...

journal_title：Cell structure and function

authors： Kunugi-Uehara Y,Hashimoto Y,Ogura C,Inagaki C,Nishino T

更新日期：1988-04-01 00:00:00

abstract：:The transcription factor ATF6 is constitutively synthesized as a type II transmembrane protein embedded in the endoplasmic reticulum (ER). When unfolded proteins accumulate in the ER, ATF6 senses such ER stress via an as yet undetermined mechanism and relocates to the Golgi apparatus where it is cleaved by sequential ...

journal_title：Cell structure and function

authors： Sato Y,Nadanaka S,Okada T,Okawa K,Mori K

更新日期：2011-01-01 00:00:00

abstract：:Immunoblots of proteins extracted from the skin of a small viviparous fish (Xiphophorus) showed that a monoclonal antibody against human urokinase recognizes multiple molecular weight species of antigens. The immunoaffinity-purified antigens had serine-protease activity for the hydrolysis of a chromogenic substrate an...

journal_title：Cell structure and function

authors： Takahashi K,Wakamatsu Y,Ozato K,Wakayama Y

更新日期：1987-02-01 00:00:00

abstract：:A method has been developed to isolate pure preparations of nuclei in high yield from commercially available viable rice embryos (germ), employing extraction with buffer solution containing glycerol (without detergent) and polyamine, followed by centrifugation on a 30% Percoll cushion. The intactness of the isolated n...

journal_title：Cell structure and function

authors： Yamaguchi J,Lim PY,Aratani K,Akazawa T

更新日期：1992-04-01 00:00:00

abstract：:The unique Physarum lysophosphatidic acid, PHYLPA, having a cyclopropane in the fatty acid moiety and a cyclic phosphate at C-2 and C-3 positions of the glycerol, inhibited proliferation of human fibroblast cells, TIG-3 and TIG-7, which were cultured in a chemically defined (serum-free) medium. The cells at S- and M-p...

journal_title：Cell structure and function

authors： Murakami-Murofushi K,Kaji K,Kano K,Fukuda M,Shioda M,Murofushi H

更新日期：1993-10-01 00:00:00

abstract：:The amount of free calcium in the cytoplasm is important in stimulation coupled with a number of cellular functions. The putative ionophoretic action of membrane lipid metabolites on Ca2+ offers convenient explanation of the stimulation-coupled mobilization of cytoplasmic Ca2+. To analyze the ionophoretic action of th...

journal_title：Cell structure and function

authors： Utsumi K,Nobori K,Terada S,Miyahara M,Utsumi T

更新日期：1985-12-01 00:00:00

abstract：:Mouse embryonic stem (mES) cells can be maintained in undifferentiated state in the presence of a cytokine, leukemia inhibitory factor (LIF). Many investigators found that STAT3 activation is important for the maintenance of pluripotency by LIF. However, the downstream pathways of STAT3 activation are still unknown. T...

journal_title：Cell structure and function

authors： Ko SY,Kang HY,Lee HS,Han SY,Hong SH

更新日期：2006-01-01 00:00:00

abstract：:We have found that 1,9-Dideoxyforskolin (DDF) strongly inhibited the cell death induced by ricin, modeccin, Pseudomonas toxin, and diphtheria toxin in MDCK cells, suggesting that these protein toxins have a DDF-sensitive common pathway leading to cell death. However, no significant effect of forskolin on these toxins ...

journal_title：Cell structure and function

authors： Oda T,Komatsu N,Muramatsu T

更新日期：1997-10-01 00:00:00

abstract：:Cells of Euglena gracilis Klebs var. bacillaris Cori mutant W3BUL grown in darkness on Hutner's pH 3.5 medium without agitation accumulate wax ester. These cells have undeveloped proplastid remnants characteristic of this mutant. If these cells are transferred to an inorganic medium and bubbled with 2-3% CO2 in air, t...

journal_title：Cell structure and function

authors： Osafune T,Schiff JA,Hase E

更新日期：1987-10-01 00:00:00

abstract：:Cholera toxin binding sites in frozen sections of rat thymus were studied by indirect immunofluorescence and were found to be localized selectively in the medulla of thymic lobules. By flow cytometric analysis, two populations of thymocytes differing in size and reactivity towards cholera toxin were detected; the smal...

journal_title：Cell structure and function

authors： Bouchon B,Iwamori M,Masago H,Nagai Y

更新日期：1987-08-01 00:00:00