Angiogenic activity of a fusion protein of the cell-binding domain of fibronectin and basic fibroblast growth factor.

abstract：:Recent studies have demonstrated that vesicle transport to cleavage furrow is indispensable for cytokinesis. Some animal and plant cells form distinct structures during cell division known as central spindle and phragmoplast, respectively. Several essential factors involved in the vesicle transport have been isolated ...

journal_title：Cell structure and function

authors： Edamatsu M

更新日期：2001-12-01 00:00:00

abstract：:The distribution of Ca2+ in dividing cells of the maize root tip was examined by potassium pyroantimonate precipitation and EGTA treatment methods. Ca2+ was found in most of the cell organelles, such as the matrix of the mitochondria, the thylakoid membrane of the proplastid and the Golgi vesicles, and on the plasma m...

journal_title：Cell structure and function

authors： Sakai-Wada A,Yagi S

更新日期：1993-12-01 00:00:00

abstract：:The discovery of fluorescent proteins (FPs) has revolutionized cell biology. The fusion of targeting sequences to FPs enables the investigation of cellular organelles and their dynamics; however, occasionally, such fluorescent fusion proteins (FFPs) exhibit behavior different from that of the native proteins. Here, we...

journal_title：Cell structure and function

authors： Kashiwagi S,Fujioka Y,Satoh AO,Yoshida A,Fujioka M,Nepal P,Tsuzuki A,Aoki O,Paudel S,Sasajima H,Ohba Y

更新日期：2019-12-26 00:00:00

abstract：:Mechanistic target of rapamycin complex 1 (mTORC1) plays a pivotal role in controlling cell growth and metabolism in response to nutrients and growth factors. The activity of mTORC1 is dually regulated by amino acids and growth factor signaling, and amino acid-dependent mTORC1 activity is regulated by mTORC1 interacti...

journal_title：Cell structure and function

authors： Ito S,Nada S,Yamazaki D,Kimura T,Kajiwara K,Miki H,Okada M

更新日期：2020-01-01 00:00:00

abstract：:DNA synthesis of WF-1 fibroblasts derived from a patient with Werner's syndrome was stimulated by fetal calf serum and adult human serum but not by various mitogens including epidermal growth factor, platelet-derived growth factor (PDGF), fibroblast growth factor, insulin and 12-O-tetradecanoylphorbol-13-acetate (TPA)...

journal_title：Cell structure and function

authors： Hirai M,Amagai M,Tajima S,Nishikawa T,Shimizu N

更新日期：1988-12-01 00:00:00

abstract：:Cultured hepatic stellate cells (HSCs) are known to change their morphology and function with respect to the production of extracellular matrices (ECMs) and matrix metalloproteinases (MMPs) in response to ECM components. We examined the regulatory role of the native form of type I collagen fibrils in pro-MMP-2 product...

journal_title：Cell structure and function

authors： Wang DR,Sato M,Li LN,Miura M,Kojima N,Senoo H

更新日期：2003-12-01 00:00:00

abstract：:Spindle pole bodies (SPBs) attached to nuclei were isolated from Saccharomyces cerevisiae; they nucleated microtubules to form asters in vitro. This aster-forming activity was stable in 30% dimethylsulfoxide or 10% 2-propanol and could be solubilized with KCl. The KCl extract contained many protein components, which a...

journal_title：Cell structure and function

authors： Yamamoto A,Nagai K,Yamasaki M,Matsuhashi M

更新日期：1990-08-01 00:00:00

abstract：:The transcription factor ATF6 is constitutively synthesized as a type II transmembrane protein embedded in the endoplasmic reticulum (ER). When unfolded proteins accumulate in the ER, ATF6 senses such ER stress via an as yet undetermined mechanism and relocates to the Golgi apparatus where it is cleaved by sequential ...

journal_title：Cell structure and function

authors： Sato Y,Nadanaka S,Okada T,Okawa K,Mori K

更新日期：2011-01-01 00:00:00

abstract：:To study the effects of surface materials of cells on the behavior of other neighboring cells in a crowded culture, confluent sheets of rat 3Y1 fibroblasts were fixed and then 3Y1 cells were seeded on to them. Among confluent sheets unfixed, fixed with formalin and fixed with ethanol and an empty plastic dish surface,...

journal_title：Cell structure and function

authors： Okuda A,Sasaki M,Kimura G

更新日期：1990-10-01 00:00:00

abstract：:Upon tryptic digestion of synaptosomes, ATPase activities decreased in the order of Cl(-)-ATPase greater than or equal to Na+,K+-ATPase greater than anion-insensitive Mg2+-ATPase. Upon synaptosome treatment with hypotonic solution, Cl(-)-ATPase or anion-insensitive Mg2+-ATPase was slightly inactivated, while Na+,K+-AT...

journal_title：Cell structure and function

authors： Kunugi-Uehara Y,Hashimoto Y,Ogura C,Inagaki C,Nishino T

更新日期：1988-04-01 00:00:00

abstract：:Infantile hemangioma is the most common soft tissue tumors in childhood. In clinic, propranolol is widely used for infantile hemangioma therapy. However, some of the infantile hemangioma patients display resistance to propranolol treatment. Previous studies show that miR-187-3p is inhibited in hepatocellular carcinoma...

journal_title：Cell structure and function

authors： Liu C,Zhao Z,Ji Z,Jiang Y,Zheng J

更新日期：2019-03-20 00:00:00

abstract：:In yeast Saccharomyces cerevisiae cells, some aberrant multimembrane-spanning proteins are not transported to the cell surface but form and are accumulated in endoplasmic reticulum (ER)-derived subcompartments, known as the ER-associated compartments (ERACs), which are observed as puncta under fluorescence microscopy....

journal_title：Cell structure and function

authors： Mai TC,Ishiwata-Kimata Y,Le QG,Kido H,Kimata Y

更新日期：2019-11-23 00:00:00

abstract：:Cofilin, a 21kDa actin-binding protein, binds to F-actin in a 1:1 molar ratio of cofilin to actin molecule (Nishida, E., S. Maekawa, and H. Sakai, Biochemistry, 23, 5307-5313, 1984) and is capable of controlling actin polymerization and depolymerization in vitro in a pH-sensitive manner (Yonezawa, N., E. Nishida, and ...

journal_title：Cell structure and function

authors： Yonezawa N,Nishida E,Koyasu S,Maekawa S,Ohta Y,Yahara I,Sakai H

更新日期：1987-10-01 00:00:00

abstract：:The fine structural localization of albumin in rat liver parenchymal cells was determined by an improved immunocytochemical method and serial sectioning. Albumin in the secretory apparatus of the parenchymal cells was present in segments of the rough endoplasmic reticulum, interrupted with negative segments, in transp...

journal_title：Cell structure and function

authors： Yokota S,Fahimi DH

更新日期：1987-06-01 00:00:00

abstract：:When human erythroleukemia cells (K562) were exposed to phorbol-12-myristate 13-acetate (PMA), phosphorylation of transferrin receptors was enhanced 5-fold with 10(-7) M PMA and 7-fold with 10(-6) M PMA, but not with 4 alpha-phorbol (5 X 10(-7) M). Stimulation took place in serine residues in the cytoplasmic domain of...

journal_title：Cell structure and function

authors： Kohno H,Taketani S,Tokunaga R

更新日期：1985-06-01 00:00:00

abstract：:Epidermal growth factor (EGF) was derivatized at the amino terminus with N-succinimidyl 3-(2-pyridyldithio)propionate and then cross-linked to the cysteinyl residues of alpha 2-macroglobulin (alpha 2M) via disulfide bonds. The EGF-alpha 2M conjugate delivered EGF into dense lysosomal fractions through binding to alpha...

journal_title：Cell structure and function

authors： Ito F,Ito S,Shimizu N

更新日期：1984-06-01 00:00:00

abstract：:Vascular endothelial cells possess antithrombotic properties, which are determined by the balance between plasminogen activators (PAs) and PA inhibitors (PAls). A cell line, TKM-33, has been established and cloned from human umbilical vein endothelial cells, was previously reported to produce a large amount of urokina...

journal_title：Cell structure and function

authors： Ueshima S,Matsumoto H,Izaki S,Mitsui Y,Fukao H,Okada K,Matsuo O

更新日期：1999-04-01 00:00:00

abstract：:We have previously shown that mouse L.P3 cells secrete fibronectin and a novel protein, gelatin-non-binding and heparin-binding cell-adhesive protein (GNCP). Here, we screened and characterized cell-adhesive proteins in the conditioned media (CM's) of a series of cell lines growing in a protein- and lipid-free synthet...

journal_title：Cell structure and function

authors： Morita H,Yasumitsu H,Watanabe Y,Miyazaki K,Umeda M

更新日期：1993-02-01 00:00:00

abstract：:Cytokeratin 8 (CK8) and cytokeratin 19 (CK19) is a specific cytoskeletal component of simple epithelia, including bronchial epithelial cells. We hypothesized that CK8 or CK19 released from epithelial cells may bind to and cause damage to extracellular matrixes through binding of anti-CK8 or anti-CK19 autoantibodies. I...

journal_title：Cell structure and function

authors： Dobashi N,Fujita J,Murota M,Ohtsuki Y,Bandoh S,Ueda Y,Dohmoto K,Hojo S,Nishioka M,Ishida T,Takahara J

更新日期：2000-06-01 00:00:00

abstract：:Ran is a nuclear Ras-like GTPase that is required for various nuclear events including the bi-directional transport of proteins and ribonucleoproteins through the nuclear pore complex, spindle formation, and reassembly of the nuclear envelope. One of the key regulators of Ran is RanGAP1, a Ran specific GTPase activati...

journal_title：Cell structure and function

authors： Takeda E,Hieda M,Katahira J,Yoneda Y

更新日期：2005-01-01 00:00:00

abstract：:The relative fibrin-binding, fibrinolytic and fibrinogenolytic properties of single-chain pro-urokinase, an inactive proenzyme form of human urokinase purified from cultured human kidney cells, and urokinase were compared. The affinity of single-chain pro-urokinase for fibrin was much higher than that of urokinase. In...

journal_title：Cell structure and function

authors： Kasai S,Arimura H,Nishida M,Suyama T

更新日期：1985-06-01 00:00:00

abstract：:Analysis of the amino acid sequence in protein (deviation analysis) suggests that the binding between trypsin and the enveloped virus is the first step of their interaction, which occurs in a specified configuration. It is possible that the distance between their active sites is important for the viral sensitivity to ...

journal_title：Cell structure and function

authors： Kiho Y,Miyata K,Okada Y

更新日期：1989-12-01 00:00:00

abstract：:Marvelous background rejection in total internal reflection fluorescence microscopy (TIR-FM) has made it possible to visualize single-fluorophores in living cells. Cell signaling proteins including peptide hormones, membrane receptors, small G proteins, cytoplasmic kinases as well as small signaling compounds have bee...

journal_title：Cell structure and function

authors： Sako Y,Uyemura T

更新日期：2002-10-01 00:00:00

abstract：:Pericentric regions form epigenetically organized, silent heterochromatin structures that accumulate histone H3 lysine 9 tri-methylation (H3K9me3) and heterochromatin protein 1 (HP1), a methylated H3K9-binding protein. At pericentric regions, Suv39h is the major enzyme that generates H3K9me3. Suv39h also interacts dir...

journal_title：Cell structure and function

authors： Muramatsu D,Kimura H,Kotoshiba K,Tachibana M,Shinkai Y

更新日期：2016-12-03 00:00:00

abstract：:Embryonic stem (ES) cells are a pluripotent cell line derived from the inner cell mass of the mouse blastocyst. The mRNA levels of several growth-related genes were examined in ES cells by Northern blot analysis under several different growth conditions. In a coculture of ES cells with the mouse fibroblast cell line, ...

journal_title：Cell structure and function

authors： Katsube K,Shimizu N

更新日期：1991-10-01 00:00:00

abstract：:tsJT60 is a nonlethal temperature-sensitive (ts) mutant of a Fischer rat cell line (3Y1) classified as a G0 mutant; i.e., the ts defect is not expressed within the cell growth cycle but is expressed only between the G0 and S phase. tsJT60 clones transformed with oncogenes such as adenovirus E1A, polyoma large T, polyo...

journal_title：Cell structure and function

authors： Tai YY,Ninomiya-Tsuji J,Furuoku K,Ogawa N,Ishibashi S,Shiroki K,Segawa K,Tsuchida N,Shibuya M,Ide T

更新日期：1990-12-01 00:00:00

abstract：:Hepatic stellate cells (HSCs) were changed in their morphology, proliferative activity, and functions by culturing on type I collagen gel, as compared to the culture on polystyrene surface. HSCs have been found to produce extracellular matrix components and matrix metalloproteinases (MMPs). In this study, we have asse...

journal_title：Cell structure and function

authors： Li YL,Sato M,Kojima N,Miura M,Senoo H

更新日期：1999-10-01 00:00:00

abstract：:Nonmuscle myosin II activity is regulated by phosphorylation of the myosin II regulatory light chain (MRLC) at Ser19 or at both Thr18 and Ser19, and the phosphorylation of MRLC promotes the contractility and stability of actomyosin. To analyze the states of MRLC phosphorylation at the leading edge in the motile HeLa c...

journal_title：Cell structure and function

authors： Uchimura T,Fumoto K,Yamamoto Y,Ueda K,Hosoya H

更新日期：2002-12-01 00:00:00

abstract：:Two types of DNA ligase, I and II, have been purified approximately 4,000-fold from mouse testes and 500-fold from nuclei of mouse spermatocytes. DNA ligase I and II consisted of single polypeptides with molecular weights of 95,000 and 65,000, respectively, according to the estimation by SDS-polyacrylamide gel electro...

journal_title：Cell structure and function

authors： Higashitani A,Tabata S,Endo H,Hotta Y

更新日期：1990-02-01 00:00:00

abstract：:A method has been developed to isolate pure preparations of nuclei in high yield from commercially available viable rice embryos (germ), employing extraction with buffer solution containing glycerol (without detergent) and polyamine, followed by centrifugation on a 30% Percoll cushion. The intactness of the isolated n...

journal_title：Cell structure and function

authors： Yamaguchi J,Lim PY,Aratani K,Akazawa T

更新日期：1992-04-01 00:00:00