Immunohistochemical localization of estradiol, progesterone, and progesterone receptor in human salivary glands and salivary adenoid cystic carcinomas.

abstract：:Phosphorylation of regulatory light chain (RMLC) of myosin II at Ser19/Thr18 is likely to play important roles in controlling the morphological changes seen during cell division of cultured mammalian cells. Phosphorylation of RMLC regulates the activity of myosin II, an essntial motor for cytokinesis, and phosphorylat...

journal_title：Cell structure and function

authors： Matsumura F,Totsukawa G,Yamakita Y,Yamashiro S

更新日期：2001-12-01 00:00:00

abstract：:We have demonstrated simultaneous measurement of the membrane potential and cytoplasmic free Ca2+ concentration ([Ca2+]i) by utilizing a dual-laser flow cytometer in embryonic rat hippocampal cell suspensions. Veratrine, a Na+ channel activator, induced both membrane depolarization and elevation of [Ca2+]i. These acti...

journal_title：Cell structure and function

authors： Mitsumoto Y,Mohri T

更新日期：1989-12-01 00:00:00

abstract：:Mouse embryonic stem (mES) cells can be maintained in undifferentiated state in the presence of a cytokine, leukemia inhibitory factor (LIF). Many investigators found that STAT3 activation is important for the maintenance of pluripotency by LIF. However, the downstream pathways of STAT3 activation are still unknown. T...

journal_title：Cell structure and function

authors： Ko SY,Kang HY,Lee HS,Han SY,Hong SH

更新日期：2006-01-01 00:00:00

abstract：:The discovery of fluorescent proteins (FPs) has revolutionized cell biology. The fusion of targeting sequences to FPs enables the investigation of cellular organelles and their dynamics; however, occasionally, such fluorescent fusion proteins (FFPs) exhibit behavior different from that of the native proteins. Here, we...

journal_title：Cell structure and function

authors： Kashiwagi S,Fujioka Y,Satoh AO,Yoshida A,Fujioka M,Nepal P,Tsuzuki A,Aoki O,Paudel S,Sasajima H,Ohba Y

更新日期：2019-12-26 00:00:00

abstract：:Effects of kinase inhibitors on the preprophase band of microtubules in onion (Allium cepa L.) root tip cells were examined. Bundled microtubules in preprophase bands were dispersed on the cell cortex when onion seedlings were incubated with 2.5-5.0 mM 6-dimethylaminopurine. Fifteen min was enough for the bundled micr...

journal_title：Cell structure and function

authors： Nogami A,Mineyuki Y

更新日期：1999-10-01 00:00:00

abstract：:The involvement of actin filaments from the host cell on the process of invasion of trypomastigote forms of Trypanosma cruzi was analyzed in seven different cell lines. Prior incubation of all cell lines with cytochalasin D, under conditions which interfere with actin filaments, markedly inhibited parasite internaliza...

journal_title：Cell structure and function

authors： Rosestolato CT,Dutra Jda M,De Souza W,de Carvalho TM

更新日期：2002-04-01 00:00:00

abstract：:We have found that 1,9-Dideoxyforskolin (DDF) strongly inhibited the cell death induced by ricin, modeccin, Pseudomonas toxin, and diphtheria toxin in MDCK cells, suggesting that these protein toxins have a DDF-sensitive common pathway leading to cell death. However, no significant effect of forskolin on these toxins ...

journal_title：Cell structure and function

authors： Oda T,Komatsu N,Muramatsu T

更新日期：1997-10-01 00:00:00

abstract：:Retinoids (retinoic acid and its analogs) are widely involved in the control of cell proliferation, cell differentiation and embryonic development. A series of potent and novel synthetic retinoids named retinobenzoic acids has been developed. Retinobenzoic acids have proven to be useful tools in the investigation of t...

journal_title：Cell structure and function

authors： Hashimoto Y

更新日期：1991-04-01 00:00:00

abstract：:Short-term and long-term (greater than 7 months) cultured astrocytes from 14-day-old rat brain were analyzed for ganglioside content. Analysis of the extracted gangliosides by HPTLC revealed that ganglioside GM1 was absent in 35 days and 235 days cultured astrocytes, and that the predominant ganglioside was GM3, showi...

journal_title：Cell structure and function

authors： Asou H,Hirano S,Uyemura K

更新日期：1989-10-01 00:00:00

abstract：:Interleukin 2 (IL-2) initiated proliferation of the cells of T-enriched population stimulated with non-mitogenic dose of concanavalin A (Con A), whereas it could not induce proliferation of the cells treated with non-mitogenic lectin wheat germ agglutinin (WGA). Use of monoclonal antibody (MAb) directed against interl...

journal_title：Cell structure and function

authors： Komada H,Nakabayashi H,Hara M,Hara M,Takahashi T,Morita J,Yoshida T,Takanari H,Izutsu K

更新日期：1989-04-01 00:00:00

abstract：:In this study, we measured hydrogen peroxide (H2O2) release as one of the functions of mature eosinophils, and utilized it as a quantitative index. We demonstrated that 1) the human eosinophilic leukemia cell line, EoL-1, did not release H2O2 when stimulated with phorbol myristate acetate (PMA), but after culturing wi...

journal_title：Cell structure and function

authors： Ohtsu H,Yamauchi K,Yoshie O,Tanno Y,Saito H,Hayashi N,Takishima T

更新日期：1993-04-01 00:00:00

abstract：:Many studies on in vitro transformation of human cells indicate that the cells must be immortalized before they can be neoplastically transformed, indicating that immortalization is a critical step in multistep neoplastic transformation of human cells. We immortalized three human cell lines by repeated treatment with ...

journal_title：Cell structure and function

authors： Mihara K,Iijima M,Kondo T,Namba M

更新日期：1996-04-01 00:00:00

abstract：:The amount of free calcium in the cytoplasm is important in stimulation coupled with a number of cellular functions. The putative ionophoretic action of membrane lipid metabolites on Ca2+ offers convenient explanation of the stimulation-coupled mobilization of cytoplasmic Ca2+. To analyze the ionophoretic action of th...

journal_title：Cell structure and function

authors： Utsumi K,Nobori K,Terada S,Miyahara M,Utsumi T

更新日期：1985-12-01 00:00:00

abstract：:Under unfavorable conditions for growth, haploid myxoamoebae of Physarum polycephalum retracted their pseudopodia and changed their cell shape into disk-like form, after which they constructed the cell walls to form microcysts. These morphological changes of haploid cells were associated with changes in intracellular ...

journal_title：Cell structure and function

authors： Shimada Y,Kasakura T,Yokota M,Miyata Y,Murofushi H,Sakai H,Yahara I,Murakami-Murofushi K

更新日期：1992-10-01 00:00:00

abstract：:The Saccharomyces cerevisiae autophagy-initiation complex, Atg1 kinase complex, consists of Atg1, Atg13, Atg17, Atg29, and Atg31, while the corresponding complex in most other eukaryotes, including mammals, is composed of ULK1 (or ULK2), Atg13, FIP200 (also known as RB1CC1), and Atg101. ULKs are homologs of Atg1, and ...

journal_title：Cell structure and function

authors： Noda NN,Mizushima N

更新日期：2016-01-01 00:00:00

abstract：:Mouse FM3A cells propagated well in serum-free medium containing 0.5% serum albumin and 1 microgram of insulin/ml. The vitamin B12 (B12) requirement of the cells depended on the population density. This requirement disappeared when a sufficiently large cell population was present. A combination of 1-100 ng of B12/ml a...

journal_title：Cell structure and function

authors： Matsuya Y,Yamane I

更新日期：1986-03-01 00:00:00

abstract：:Embryonic stem (ES) cells are a pluripotent cell line derived from the inner cell mass of the mouse blastocyst. The mRNA levels of several growth-related genes were examined in ES cells by Northern blot analysis under several different growth conditions. In a coculture of ES cells with the mouse fibroblast cell line, ...

journal_title：Cell structure and function

authors： Katsube K,Shimizu N

更新日期：1991-10-01 00:00:00

abstract：:The Acanthamoeba myosin-IA and myosin-IB molecular motors bind to membranes, so they may produce the force to move organelles and membranes along actin filaments. We have determined the rate constants for the actin-activated myosin-I ATPase by pre-steady state kinetic analysis. ATP binds rapidly to myosin-I and dissoc...

journal_title：Cell structure and function

authors： Pollard TD,Ostap EM

更新日期：1996-10-01 00:00:00

abstract：:It has been suggested that the organization of microtubules during mitosis plays an important role in cytokinesis in animal cells. We studied the organization of microtubules during the first cleavage and its role in cytokinesis of Xenopus eggs. First, we examined the immunofluorescent localization of microtubules in ...

journal_title：Cell structure and function

authors： Takayama M,Noguchi T,Yamashiro S,Mabuchi I

更新日期：2002-08-01 00:00:00

abstract：:In the mitotic sea urchin egg, the spindle microtubules were composed of different tubulin isotypes from those of astral microtubules using monoclonal antibodies [Oka et al. (1990) Cell Motil. Cytoskeleton, 16, 239-250]. Three of the antibodies, D2D6, DM1B, and YL1/2, were specific for spindle microtubules, astral mic...

journal_title：Cell structure and function

authors： Oka MT,Arai T,Hamaguchi Y

更新日期：1991-04-01 00:00:00

abstract：:Epidermal growth factor (EGF) was derivatized at the amino terminus with N-succinimidyl 3-(2-pyridyldithio)propionate and then cross-linked to the cysteinyl residues of alpha 2-macroglobulin (alpha 2M) via disulfide bonds. The EGF-alpha 2M conjugate delivered EGF into dense lysosomal fractions through binding to alpha...

journal_title：Cell structure and function

authors： Ito F,Ito S,Shimizu N

更新日期：1984-06-01 00:00:00

abstract：:Checkpoint kinase 1 (Chk1) is a conserved protein kinase central to the cell-cycle checkpoint during DNA damage response (DDR). Until recently, ATR, a protein kinase activated in response to DNA damage or stalled replication, has been considered as the sole regulator of Chk1. Recent progress, however, has led to the i...

journal_title：Cell structure and function

authors： Goto H,Kasahara K,Inagaki M

更新日期：2015-01-01 00:00:00

abstract：:DNA-dependent protein kinase (DNA-PK), consisting of the 470-kDa catalytic component (DNA-PKcs) and the DNA-binding regulatory component Ku protein (p70/p80), catalyzes phosphorylation of a variety of DNA replication/transcription/repair factors in the presence of double-stranded DNA. In the resting states of human pe...

journal_title：Cell structure and function

authors： Nagasawa M,Watanabe F,Suwa A,Yamamoto K,Tsukada K,Teraoka H

更新日期：1997-12-01 00:00:00

abstract：:The putative ionophoretic action of phosphatidic acid or arachidonic acid metabolites for Ca2+ has offered an attractive explanation for stimulation-coupled mobilization of cytoplasmic Ca2+. We have examined the effects of Ca2+ ionophore and long-chain unsaturated fatty acids on the translocation of Ca2+ across the li...

journal_title：Cell structure and function

authors： Utsumi K,Nobori K,Miyahara M

更新日期：1985-06-01 00:00:00

abstract：:Hepatic stellate cells (HSCs), a mesenchymal cell type in hepatic parenchyma, have unique features with respect to their cellular origin, morphology, and function. Normal, quiescent HSCs function as major vitamin A-storing cells containing over 80% of total vitamin A in the body to maintain vitamin A homeostasis. HSCs...

journal_title：Cell structure and function

authors： Sato M,Suzuki S,Senoo H

更新日期：2003-04-01 00:00:00

abstract：:An immunocytochemical method using a specific monoclonal antibody was employed to detect DNA polymerase alpha in Drosophila melanogaster embryos during the first 13 nuclear division cycles after fertilization. The anti-DNA polymerase alpha antibody stained the ooplasm of the unfertilized egg, indicating that DNA polym...

journal_title：Cell structure and function

authors： Yamaguchi M,Kuroda K,Hirose F,Matsukage A

更新日期：1992-04-01 00:00:00

abstract：:The amount of intact 40S rRNA precursor was followed by Northern hybridization in the course of the early embryogenesis of wild-type Xenopus laevis and its anucleolate mutant. The total amount of 40S rRNA precursor did not alter appreciably until the midblastula stage, decreased at the late blastula stage, and then in...

journal_title：Cell structure and function

authors： Furuno N,Shiokawa K,Kobayashi H

更新日期：1988-04-01 00:00:00

abstract：:All trans retinoic acid (ATRA), a differentiation inducer for human myeloid NB4 cells, induced accumulation of lipid droplet as determined by positivity of Nile Red and Oil Red O in this cell line. Granulocyte colony-stimulating factor (G-CSF), although not having detectable effect by itself, exerted the additive effe...

journal_title：Cell structure and function

authors： Inazawa Y,Nakatsu M,Yasugi E,Saeki K,Yuo A

更新日期：2003-10-01 00:00:00

abstract：:A voltage-dependent and Ca2(+)-activated cation channel recently found in the vacuolar membrane of the yeast Saccharomyces cerevisiae was incorporated into planar lipid bilayers and further characterized in macroscopic and single channel levels. Single channel conductances for various cations were in the order: NH4+ g...

journal_title：Cell structure and function

authors： Sato M,Tanifuji M,Kasai M

更新日期：1989-12-01 00:00:00

abstract：:Thyroid follicles in vivo are embedded in extracellular matrix (ECM). The composing epithelial cells are in close contact with ECM at the basal side. To examine cell-to-ECM interactions, we studied adhesion, proliferation and differentiation of porcine follicle cells monolayer-cultured on type I and IV collagen, fibro...

journal_title：Cell structure and function

authors： Toda S,Matsumura S,Yonemitsu N,Fujitani N,Terayama K,Funatsumaru S,Sugihara H

更新日期：1995-10-01 00:00:00