c-myc induces autophagy in rat 3Y1 fibroblast cells.

abstract：:Here we describe the design and synthesis of a bifunctional two-photon fluorescence probe, N,N'-‍dimethyl-4,4'-(biphenyl-2,1-ethenediyl)dipyridinium hexafluorophosphate (BP6). HeLa, Hek293, and Paramecium caudatum cells were stained with BP6. BP6 accumulated on the mitochondria of all three cell types when the mitocho...

journal_title：Cell structure and function

authors： Moritomo H,Yamada K,Kojima Y,Suzuki Y,Tani S,Kinoshita H,Sasaki A,Mikuni S,Kinjo M,Kawamata J

更新日期：2014-01-01 00:00:00

abstract：:Here, we would like to point out important milestones in the study of nuclear radial positioning and gene expression during differentiation processes. In addition, changes in the histone signature that significantly precede various differentiation pathways are reviewed. We address the regulatory functions of chromatin...

journal_title：Cell structure and function

authors： Harnicarová Horáková A,Bártová E,Kozubek S

更新日期：2010-01-01 00:00:00

abstract：:To investigate the mechanism of myogenic differentiation, we are using quail myoblast cells (QM cells) transformed with a temperature-sensitive mutant of Rous sarcoma virus (ts-RSV), termed QM-RSV cells. At 35.5 degrees C, a permissive temperature for RSV, QM-RSV cells repeatedly proliferate without differentiation, b...

journal_title：Cell structure and function

authors： Hase H,Hirayama E,Kim J

更新日期：1996-02-01 00:00:00

abstract：:1. Cofilin is an essential actin-regulating protein widely distributed in all eucaryotes. The structure and function of cofilin are conserved during evolution. 2. Cofilin depolymerizes F-actin in vitro at alkaline pH and severs F-actin in vitro at pH lower than 7.3. Overexpression of cofilin in viable cells induced bu...

journal_title：Cell structure and function

authors： Yahara I,Aizawa H,Moriyama K,Iida K,Yonezawa N,Nishida E,Hatanaka H,Inagaki F

更新日期：1996-10-01 00:00:00

abstract：:To study the effects of surface materials of cells on the behavior of other neighboring cells in a crowded culture, confluent sheets of rat 3Y1 fibroblasts were fixed and then 3Y1 cells were seeded on to them. Among confluent sheets unfixed, fixed with formalin and fixed with ethanol and an empty plastic dish surface,...

journal_title：Cell structure and function

authors： Okuda A,Sasaki M,Kimura G

更新日期：1990-10-01 00:00:00

abstract：:Checkpoint kinase 1 (Chk1) is a conserved protein kinase central to the cell-cycle checkpoint during DNA damage response (DDR). Until recently, ATR, a protein kinase activated in response to DNA damage or stalled replication, has been considered as the sole regulator of Chk1. Recent progress, however, has led to the i...

journal_title：Cell structure and function

authors： Goto H,Kasahara K,Inagaki M

更新日期：2015-01-01 00:00:00

abstract：:In this study, we measured hydrogen peroxide (H2O2) release as one of the functions of mature eosinophils, and utilized it as a quantitative index. We demonstrated that 1) the human eosinophilic leukemia cell line, EoL-1, did not release H2O2 when stimulated with phorbol myristate acetate (PMA), but after culturing wi...

journal_title：Cell structure and function

authors： Ohtsu H,Yamauchi K,Yoshie O,Tanno Y,Saito H,Hayashi N,Takishima T

更新日期：1993-04-01 00:00:00

abstract：:A strain of Saccharomyces cerevisiae that contains an integrated copy of a Xenopus cyclin A1 gene under the control of the GAL1 promoter has been constructed. On inducing expression of cyclin A1, the nuclear migration that occurs prior to division becomes aberrant. Instead of migrating to the neck between the mother c...

journal_title：Cell structure and function

authors： Sikder H,Funakoshi M,Nishimoto T,Kobayashi H

更新日期：1997-08-01 00:00:00

abstract：:Primary hepatocytes were cultured on collagen gel in serum-free, alpha-modified Eagle's minimum essential medium containing 0.1 microM insulin, 0.1 microM dexamethasone, 10 mM pyruvate and supplements such as glucagon, epinephrine or growth hormone. The activities of alkaline phosphatase, 5'-nucleotidase and gamma-glu...

journal_title：Cell structure and function

authors： Horiuti Y,Yano K,Shibuya Y

更新日期：1993-02-01 00:00:00

abstract：:Many studies on in vitro transformation of human cells indicate that the cells must be immortalized before they can be neoplastically transformed, indicating that immortalization is a critical step in multistep neoplastic transformation of human cells. We immortalized three human cell lines by repeated treatment with ...

journal_title：Cell structure and function

authors： Mihara K,Iijima M,Kondo T,Namba M

更新日期：1996-04-01 00:00:00

abstract：:Two types of DNA ligase, I and II, have been purified approximately 4,000-fold from mouse testes and 500-fold from nuclei of mouse spermatocytes. DNA ligase I and II consisted of single polypeptides with molecular weights of 95,000 and 65,000, respectively, according to the estimation by SDS-polyacrylamide gel electro...

journal_title：Cell structure and function

authors： Higashitani A,Tabata S,Endo H,Hotta Y

更新日期：1990-02-01 00:00:00

abstract：:Staurosporine has been reported to cause arrest of cells in G1 phase at low concentration and in G2 phase at high concentration. This raises the question of why the effects of staurosporine on the cell cycle depend on the applied concentration. In order to verify these multiple functions of staurosporine in Meth-A cel...

journal_title：Cell structure and function

authors： Zong ZP,Fujikawa-Yamamoto K,Li AL,Yamaguchi N,Chang YG,Murakami M,Odashima S,Ishikawa Y

更新日期：1999-12-01 00:00:00

abstract：:Two monoclonal antibodies against alpha-tubulin (YL1/2 and D2D6) were microinjected into the egg of the sand dollar Clypeaster japonicus, and their effects on cleavage of the egg were investigated. They had already been shown by immunoblotting to react specifically with egg tubulin and by immunofluorescence to stain t...

journal_title：Cell structure and function

authors： Oka MT,Arai T,Hamaguchi Y

更新日期：1990-12-01 00:00:00

abstract：:The precise control of spindle microtubule (MT) dynamics is essential for chromosome capture and alignment. Kif18A/kinesin-8, an essential regulator of kinetochore MT dynamics, accumulates at its plus-ends in metaphase but not prometaphase cells. The underlying mechanism of time-dependent and kinetochore MT-specific p...

journal_title：Cell structure and function

authors： Masuda N,Shimodaira T,Shiu SJ,Tokai-Nishizumi N,Yamamoto T,Ohsugi M

更新日期：2011-01-01 00:00:00

abstract：:The endolymphatic sac (ES) is believed to play an important role in maintaining homeostasis in the inner ear by the absorption and endocytosis of endolymph. Megalin is a 600-kDa multiligand endocytic receptor expressed in certain types of absorptive epithelia including kidney proximal tubules. We analyzed the immunore...

journal_title：Cell structure and function

authors： Ishida T,Hatae T,Nishi N,Araki N

更新日期：2006-01-01 00:00:00

abstract：:In the present study, we compared the abilities of ricin and diphtheria toxin to induce apoptosis in Vero cells. The cytolysis and DNA fragmentation by ricin paralleled its protein synthesis inhibitory activity. However, unlike ricin, diphtheria toxin could induce neither cytolysis nor DNA fragmentation in Vero cells ...

journal_title：Cell structure and function

authors： Kusano I,Kageyama A,Tamura T,Oda T,Muramatsu T

更新日期：2001-10-01 00:00:00

abstract：:Multiple type I and II hair keratins are expressed in hair-forming cells but the role of each protein in hair fiber formation remains obscure. In this study, recombinant proteins of human type I hair keratins (K35, K36 and K38) and type II hair keratins (K81 and K85) were prepared using bacterial expression systems. T...

journal_title：Cell structure and function

authors： Honda Y,Koike K,Kubo Y,Masuko S,Arakawa Y,Ando S

更新日期：2014-01-01 00:00:00

abstract：:Cholera toxin binding sites in frozen sections of rat thymus were studied by indirect immunofluorescence and were found to be localized selectively in the medulla of thymic lobules. By flow cytometric analysis, two populations of thymocytes differing in size and reactivity towards cholera toxin were detected; the smal...

journal_title：Cell structure and function

authors： Bouchon B,Iwamori M,Masago H,Nagai Y

更新日期：1987-08-01 00:00:00

abstract：:All trans retinoic acid (ATRA), a differentiation inducer for human myeloid NB4 cells, induced accumulation of lipid droplet as determined by positivity of Nile Red and Oil Red O in this cell line. Granulocyte colony-stimulating factor (G-CSF), although not having detectable effect by itself, exerted the additive effe...

journal_title：Cell structure and function

authors： Inazawa Y,Nakatsu M,Yasugi E,Saeki K,Yuo A

更新日期：2003-10-01 00:00:00

abstract：:Short-term and long-term (greater than 7 months) cultured astrocytes from 14-day-old rat brain were analyzed for ganglioside content. Analysis of the extracted gangliosides by HPTLC revealed that ganglioside GM1 was absent in 35 days and 235 days cultured astrocytes, and that the predominant ganglioside was GM3, showi...

journal_title：Cell structure and function

authors： Asou H,Hirano S,Uyemura K

更新日期：1989-10-01 00:00:00

abstract：:High voltage electric pulses, 1000 V/cm, were found to induce cell fusion efficiently when delivered to cells growing in a monolayer culture. The maximum yield of fused cells was about 30% of cells remaining after treatment. Colonies of interspecies hybrid cells between mouse and others also appeared with a frequency ...

journal_title：Cell structure and function

authors： Ishizaki K,Chang HR,Eguchi T,Ikenaga M

更新日期：1989-04-01 00:00:00

abstract：:Marvelous background rejection in total internal reflection fluorescence microscopy (TIR-FM) has made it possible to visualize single-fluorophores in living cells. Cell signaling proteins including peptide hormones, membrane receptors, small G proteins, cytoplasmic kinases as well as small signaling compounds have bee...

journal_title：Cell structure and function

authors： Sako Y,Uyemura T

更新日期：2002-10-01 00:00:00

abstract：:It has been suggested that the organization of microtubules during mitosis plays an important role in cytokinesis in animal cells. We studied the organization of microtubules during the first cleavage and its role in cytokinesis of Xenopus eggs. First, we examined the immunofluorescent localization of microtubules in ...

journal_title：Cell structure and function

authors： Takayama M,Noguchi T,Yamashiro S,Mabuchi I

更新日期：2002-08-01 00:00:00

abstract：:A large number of Rab/Ypt-family small GTPases have been identified from higher plants. While some of them can complement yeast ypt mutants, the expression of Arabidopsis Ara4 protein aggravated the growth defect of a subset of ypt mutants, probably because of the titration of common regulator(s) of yeast Ypt proteins...

journal_title：Cell structure and function

authors： Matsuda N,Ueda T,Sasaki Y,Nakano A

更新日期：2000-02-01 00:00:00

abstract：:Immunoblots of proteins extracted from the skin of a small viviparous fish (Xiphophorus) showed that a monoclonal antibody against human urokinase recognizes multiple molecular weight species of antigens. The immunoaffinity-purified antigens had serine-protease activity for the hydrolysis of a chromogenic substrate an...

journal_title：Cell structure and function

authors： Takahashi K,Wakamatsu Y,Ozato K,Wakayama Y

更新日期：1987-02-01 00:00:00

abstract：:NBT-II cells on collagen-coated substrates move rapidly and persistently, maintaining a semi-circular shape with a large lamellipodium, in a manner similar to fish keratocytes. The inhibitor of phospholipase D (PLD), n-butanol, completely blocked the migration and disturbed the characteristic localization of actin alo...

journal_title：Cell structure and function

authors： Nagasaki A,Inotsume K,Kanada M,Uyeda TQ

更新日期：2008-01-01 00:00:00

abstract：:tsJT60 is a nonlethal temperature-sensitive (ts) mutant of a Fischer rat cell line (3Y1) classified as a G0 mutant; i.e., the ts defect is not expressed within the cell growth cycle but is expressed only between the G0 and S phase. tsJT60 clones transformed with oncogenes such as adenovirus E1A, polyoma large T, polyo...

journal_title：Cell structure and function

authors： Tai YY,Ninomiya-Tsuji J,Furuoku K,Ogawa N,Ishibashi S,Shiroki K,Segawa K,Tsuchida N,Shibuya M,Ide T

更新日期：1990-12-01 00:00:00

abstract：:Effects of kinase inhibitors on the preprophase band of microtubules in onion (Allium cepa L.) root tip cells were examined. Bundled microtubules in preprophase bands were dispersed on the cell cortex when onion seedlings were incubated with 2.5-5.0 mM 6-dimethylaminopurine. Fifteen min was enough for the bundled micr...

journal_title：Cell structure and function

authors： Nogami A,Mineyuki Y

更新日期：1999-10-01 00:00:00

abstract：:The involvement of actin filaments from the host cell on the process of invasion of trypomastigote forms of Trypanosma cruzi was analyzed in seven different cell lines. Prior incubation of all cell lines with cytochalasin D, under conditions which interfere with actin filaments, markedly inhibited parasite internaliza...

journal_title：Cell structure and function

authors： Rosestolato CT,Dutra Jda M,De Souza W,de Carvalho TM

更新日期：2002-04-01 00:00:00

abstract：:The distribution of Ca2+ in dividing cells of the maize root tip was examined by potassium pyroantimonate precipitation and EGTA treatment methods. Ca2+ was found in most of the cell organelles, such as the matrix of the mitochondria, the thylakoid membrane of the proplastid and the Golgi vesicles, and on the plasma m...

journal_title：Cell structure and function

authors： Sakai-Wada A,Yagi S

更新日期：1993-12-01 00:00:00