Purification and identification of pituitary cytotropic factor.

abstract：:Spatial working memory or short-term place memory is impaired in schizophrenia. The efficiency of antipsychotic drugs, particularly of typical antipsychotics, on cognitive deficit in schizophrenia remains disputable. Inhibition of serotonin (5-HT) 2A/2C receptors is important for cognitive improvement in schizophrenic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bubenikova-Valesova V,Stuchlik A,Svoboda J,Bures J,Vales K

更新日期：2008-01-22 00:00:00

abstract：:Whole human blood was examined by (31)P nuclear magnetic resonance spectroscopy. Individual phosphates (alpha,beta,gamma) of ATP were identifiable, and two microenvironments appeared to be present for this molecule. When sequential recordings of freshly collected blood were made, 2,3-diphosphoglycerate was observed to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Henderson TO,Costello AJ,Omachi A

更新日期：1974-06-01 00:00:00

abstract：:Ig class-switch recombination (CSR) is directed by the long and repetitive switch regions and requires activation-induced cytidine deaminase (AID). One of the conserved switch-region sequence motifs (AGCT) is a preferred site for AID-mediated DNA-cytosine deamination. By using somatic gene targeting and recombinase-me...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Han L,Masani S,Yu K

更新日期：2011-07-12 00:00:00

abstract：:The sporulation of Saccharomyces cerevisiae in the presence of [2-13C]acetate was studied by 13C NMR spectroscopy. The fate of 13C label was analyzed in vivo and in cell extracts. During the first 4 hr of sporulation the major metabolite produced from [2-13C]acetate utilization was glutamate. From the labeling pattern...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dickinson JR,Dawes IW,Boyd AS,Baxter RL

更新日期：1983-10-01 00:00:00

abstract：:Somatic crossing over in flies heterozygous for a dominant Minute mutant may result in two cells, homozygous for wild type and Minute, respectively. If both cells took part in development, a twin spot of two genotypes, different from the rest of the body, would result. Two-hundred wild type spots were found, none of w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stern C,Tokunaga C

更新日期：1971-02-01 00:00:00

abstract：:Plasmodium parasites must complete development in the mosquito vector for transmission to occur. The mosquito innate immune response is remarkably efficient in limiting parasite numbers. Previous work has identified a LPS-induced TNFα transcription factor (LITAF)-like transcription factor, LITAF-like 3 (LL3), which si...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith RC,Barillas-Mury C,Jacobs-Lorena M

更新日期：2015-06-30 00:00:00

abstract：:In response to the challenges of cancer chemotherapeutics, including poor physicochemical properties, low tumor targeting, insufficient tumor cell internalization/bioavailability, and side effects, we developed a unique tumor-targeted micellar drug-delivery platform. Using paclitaxel as a model therapeutic, a nanoprep...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhu L,Wang T,Perche F,Taigind A,Torchilin VP

更新日期：2013-10-15 00:00:00

abstract：:The hormone gibberellin (GA) is a key regulator of plant growth. Many of the components of the gibberellin signal transduction [e.g., GIBBERELLIN INSENSITIVE DWARF 1 (GID1) and DELLA], biosynthesis [e.g., GA 20-oxidase (GA20ox) and GA3ox], and deactivation pathways have been identified. Gibberellin binds its receptor,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Middleton AM,Úbeda-Tomás S,Griffiths J,Holman T,Hedden P,Thomas SG,Phillips AL,Holdsworth MJ,Bennett MJ,King JR,Owen MR

更新日期：2012-05-08 00:00:00

abstract：:Origins of DNA replication in Schizosaccharomyces pombe lack a specific consensus sequence analogous to the Saccharomyces cerevisiae autonomously replicating sequence (ARS) consensus, raising the question of how they are recognized by the replication machinery. Because all well characterized S. pombe origins are locat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dai J,Chuang RY,Kelly TJ

更新日期：2005-01-11 00:00:00

abstract：:Receptor-mediated endocytosis proceeds by transfer of receptor-ligand complexes from clathrin-coated pits at the cell surface to uncoated endocytic vesicles termed receptosomes (or endosomes). These vesicles have now been purified more than 37-fold based on their content of newly internalized epidermal growth factor. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dickson RB,Beguinot L,Hanover JA,Richert ND,Willingham MC,Pastan I

更新日期：1983-09-01 00:00:00

abstract：:The functional role of residue Tyr-19 of Chromatium vinosum HiPIP has been evaluated by site-directed mutagenesis experiments. The stability of the [Fe4S4] cluster prosthetic center is sensitive to side-chain replacements. Polar residues result in significant instability, while nonpolar residues (especially with aroma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Agarwal A,Li D,Cowan JA

更新日期：1995-10-10 00:00:00

abstract：:Over the past 50 y, behavioral experiments have produced a large body of evidence for the existence of a magnetic sense in a wide range of animals. However, the underlying sensory physiology remains poorly understood due to the elusiveness of the magnetosensory structures. Here we present an effective method for isola...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eder SH,Cadiou H,Muhamad A,McNaughton PA,Kirschvink JL,Winklhofer M

更新日期：2012-07-24 00:00:00

abstract：:Syringomycin, a peptide toxin and a virulence factor produced by the bacterial phytopathogen Pseudomonas syringae pv. syringae, stimulated the phosphorylation of several plasma membrane polypeptides of red beet storage tissue. Among these was a 100-kDa polypeptide, which corresponds in size to the proton pump ATPase. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bidwai AP,Takemoto JY

更新日期：1987-10-01 00:00:00

abstract：:Species- and strain-specific spontaneously occurring tumors have been observed in rodents maintained under normal laboratory conditions. Elucidation of the molecular mechanisms associated with the development of these spontaneous tumors may provide a better understanding of tumor development associated with exposure t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reynolds SH,Stowers SJ,Maronpot RR,Anderson MW,Aaronson SA

更新日期：1986-01-01 00:00:00

abstract：:All extant life employs the same 20 amino acids for protein biosynthesis. Studies on the number of amino acids necessary to produce a foldable and catalytically active polypeptide have shown that a basis set of 7-13 amino acids is sufficient to build major structural elements of modern proteins. Hence, the reasons for...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Granold M,Hajieva P,Toşa MI,Irimie FD,Moosmann B

更新日期：2018-01-02 00:00:00

abstract：:Ionotropic alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors (iGluRs) mediate the majority of excitatory synaptic transmission in the CNS and are essential for the induction and maintenance of long-term potentiation and long-term depression, two cellular models of learning and memory. We identi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu Y,Arai AC,Rumbaugh G,Srivastava AK,Turner G,Hayashi T,Suzuki E,Jiang Y,Zhang L,Rodriguez J,Boyle J,Tarpey P,Raymond FL,Nevelsteen J,Froyen G,Stratton M,Futreal A,Gecz J,Stevenson R,Schwartz CE,Valle D,Huganir

更新日期：2007-11-13 00:00:00

abstract：:Human type O erythrocyte membranes were converted to type A1 by purified human A1-enzyme, to type A2 by purified human A2-enzyme, and to type B by purified human B-enzyme in the presence of radioactive sugar donors (i.e., UDP-N-acetylgalactosamine for A-enzyme and UDP-galactose for B-enzyme, respectively). Type A2 ery...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fujii H,Yoshida A

更新日期：1980-05-01 00:00:00

abstract：:After encoding, memory traces are initially fragile and have to be reinforced to become permanent. The initial steps of this process occur at a cellular level within minutes or hours. Besides this rapid synaptic consolidation, systems consolidation occurs within a time frame of days to years. For declarative memory, t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gais S,Albouy G,Boly M,Dang-Vu TT,Darsaud A,Desseilles M,Rauchs G,Schabus M,Sterpenich V,Vandewalle G,Maquet P,Peigneux P

更新日期：2007-11-20 00:00:00

abstract：:A system for studying Z-DNA formation in the Escherichia coli chromosome was developed. Prior investigations in recombinant plasmids showed that alternating (Pur-Pyr) sequences can adopt a left-handed Z-DNA conformation both in vitro and in vivo. We constructed mobile, transposon-based cassettes carrying cloned (Pur-P...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lukomski S,Wells RD

更新日期：1994-10-11 00:00:00

abstract：:The exocytosis of AMPA receptors is a key step in long-term potentiation (LTP), yet the timing and location of exocytosis and the signaling pathways involved in exocytosis during synaptic plasticity are not fully understood. Here we combine two-photon uncaging with two-photon imaging of a fluorescent label of surface ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Patterson MA,Szatmari EM,Yasuda R

更新日期：2010-09-07 00:00:00

abstract：:The ability to intercept uncooperative targets is key to many diverse flight behaviors, from courtship to predation. Previous research has looked for simple geometric rules describing the attack trajectories of animals, but the underlying feedback laws have remained obscure. Here, we use GPS loggers and onboard video ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brighton CH,Thomas ALR,Taylor GK

更新日期：2017-12-19 00:00:00

abstract：:One of the ultimate objectives of de novo protein design is to realize systems capable of catalyzing redox reactions on substrates. This goal is challenging as redox-active proteins require design considerations for both the reduced and oxidized states of the protein. In this paper, we describe the spectroscopic chara...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tegoni M,Yu F,Bersellini M,Penner-Hahn JE,Pecoraro VL

更新日期：2012-12-26 00:00:00

abstract：:After sublethal (6 Gy) whole-body irradiation, the C57BL/Ba (Thy-1.1) murine thymus regenerated in two waves, on days 3-10 and 25-32, separated by a severe relapse. The second phase of depletion-reconstitution reproduced the first one, in a less synchronous manner. The depletion affected all cell subsets, but CD4+ CD8...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Penit C,Ezine S

更新日期：1989-07-01 00:00:00

abstract：:Shewanella species are renowned for their respiratory versatility, including their ability to respire poorly soluble substrates by using enzymatic machinery that is localized to the outside of the cell. The ability to engage in "extracellular respiration" to date has focused primarily on respiration of minerals. Here,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gralnick JA,Vali H,Lies DP,Newman DK

更新日期：2006-03-21 00:00:00

abstract：:Lactate racemase is the first enzyme known to possess a metal pincer active site. The enzyme interconverts d- and l-lactic acid, which is important for the assembly of cell walls in many microorganisms. Here, we report a synthetic model of the active site of lactate racemase, which features a pyridinium-based SCS pinc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu T,Wodrich MD,Scopelliti R,Corminboeuf C,Hu X

更新日期：2017-02-07 00:00:00

abstract：:Notch (N) is a transmembrane receptor that mediates cell-cell interactions to determine many cell-fate decisions. N contains EGF-like repeats, many of which have an O-fucose glycan modification that regulates N-ligand binding. This modification requires GDP-L-fucose as a donor of fucose. The GDP-L-fucose biosynthetic ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ayukawa T,Matsumoto K,Ishikawa HO,Ishio A,Yamakawa T,Aoyama N,Suzuki T,Matsuno K

更新日期：2012-09-18 00:00:00

abstract：:The recent characterization of the prokaryotic Cys(2)His(2) zinc-finger domain, identified in Ros protein from Agrobacterium tumefaciens, has demonstrated that, although possessing a similar zinc coordination sphere, this domain is structurally very different from its eukaryotic counterpart. A search in the databases ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baglivo I,Russo L,Esposito S,Malgieri G,Renda M,Salluzzo A,Di Blasio B,Isernia C,Fattorusso R,Pedone PV

更新日期：2009-04-28 00:00:00

abstract：:A peptide containing 31 amino acids with inhibin-like activity has been synthesized by the solid-phase method. The synthetic product was shown to be homogeneous in high-performance liquid chromatography and paper electrophoresis. In the mouse pituitary incubation system, the follitropin-suppressing activity of the syn...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamashiro D,Li CH,Ramasharma K,Sairam MR

更新日期：1984-09-01 00:00:00

abstract：:Cerebellar long-term depression (LTD) is a persistent attenuation of synaptic transmission at the parallel fiber-Purkinje cell synapse mediated by the removal of GluR2 subunit-containing alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors. The removal of AMPA receptors requires protein kinase C p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Steinberg JP,Huganir RL,Linden DJ

更新日期：2004-12-28 00:00:00

abstract：:Energy relaxation in light-harvesting complexes has been extensively studied by various ultrafast spectroscopic techniques, the fastest processes being in the sub-100-fs range. At the same time, much slower dynamics have been observed in individual complexes by single-molecule fluorescence spectroscopy (SMS). In this ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Malý P,Gruber JM,Cogdell RJ,Mančal T,van Grondelle R

更新日期：2016-03-15 00:00:00