当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > DNA replication origins in the Schizosaccharomyces pombe genome.

DNA replication origins in the Schizosaccharomyces pombe genome.

Abstract：

:Origins of DNA replication in Schizosaccharomyces pombe lack a specific consensus sequence analogous to the Saccharomyces cerevisiae autonomously replicating sequence (ARS) consensus, raising the question of how they are recognized by the replication machinery. Because all well characterized S. pombe origins are located in intergenic regions, we analyzed the sequence properties and biological activity of such regions. The AT content of intergenes is very high ( approximately 70%), and runs of A's or T's occur with a significantly greater frequency than expected. Additionally, the two DNA strands in intergenes display compositional asymmetry that strongly correlates with the direction of transcription of flanking genes. Importantly, the sequence properties of known S. pombe origins of DNA replication are similar to those of intergenes in general. In functional studies, we assayed the in vivo origin activity of 26 intergenes in a 68-kb region of S. pombe chromosome 2. We also assayed the origin activity of sets of randomly chosen intergenes with the same length or AT content. Our data demonstrate that at least half of intergenes have potential origin activity and that the relative ability of an intergene to function as an origin is governed primarily by AT content and length. We propose a stochastic model for initiation of DNA replication in the fission yeast. In this model, the number of AT tracts in a given sequence is the major determinant of its probability of binding SpORC and serving as a replication origin. A similar model may explain some features of origins of DNA replication in metazoans.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Dai J,Chuang RY,Kelly TJ

doi

10.1073/pnas.0408811102

keywords:

subject

Has Abstract

pub_date

2005-01-11 00:00:00

pages

337-42

issue

2

eissn

0027-8424

issn

1091-6490

pii

0408811102

journal_volume

102

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Translocation of intracellular glutathione to membrane-bound gamma-glutamyl transpeptidase as a discrete step in the gamma-glutamyl cycle: glutathionuria after inhibition of transpeptidase.

    abstract：:Several inhibitors of gamma-glutamyl transpeptidase in vitro [L-serine plus borate, 6-diazo-5-oxo-L-norleucine, and L- and D-gamma-glutamyl-(o-carboxy)phenylhydrazide] are active in vivo, as indicated by their effect in decreasing the conversion of administered D-gamma-glutamyl-L-alpha-amino[(14)C]butyrate to respirat...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.76.1.268

    authors： Griffith OW,Meister A

    更新日期：1979-01-01 00:00:00

  • Myoglobin-mediated oxygen delivery to mitochondria of isolated cardiac myocytes.

    abstract：:Myoglobin-mediated oxygen delivery to intracellular mitochondria is demonstrated in cardiac myocytes isolated from the hearts of mature rats. Myocytes are held at high ambient oxygen pressure, 40-340 torr (5-45 kPa); sarcoplasmic myoglobin is fully oxygenated. In this condition oxygen availability does not limit respi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.21.7503

    authors： Wittenberg BA,Wittenberg JB

    更新日期：1987-11-01 00:00:00

  • Superdiffusive transport of energy in one-dimensional metals.

    abstract：:Metals in one spatial dimension are described at the lowest energy scales by the Luttinger liquid theory. It is well understood that this free theory, and even interacting integrable models, can support ballistic transport of conserved quantities including energy. In contrast, realistic one-dimensional metals, even wi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1916213117

    authors： Bulchandani VB,Karrasch C,Moore JE

    更新日期：2020-06-09 00:00:00

  • A biosensor assay for studying ligand-membrane receptor interactions: binding of antibodies and HIV-1 Env to chemokine receptors.

    abstract：:The HIV envelope (Env) protein mediates entry into cells by binding CD4 and an appropriate coreceptor, which triggers structural changes in Env that lead to fusion between the viral and cellular membranes. The major HIV-1 coreceptors are the seven transmembrane domain chemokine receptors CCR5 and CXCR4. The type of co...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.190274097

    authors： Hoffman TL,Canziani G,Jia L,Rucker J,Doms RW

    更新日期：2000-10-10 00:00:00

  • Investigation of phase transitions of lipids and lipid mixtures by sensitivity differential scanning calorimetry.

    abstract：:High sensitivity differential scanning calorimetry is applied to the study of the thermotropic behavior of mixtures of synthetic phospholipids in multilamellar aqueous suspensions. The systems dimyristoylphosphatidylcholine dipalmitoylphosphatidylcholine, and dimyristoylphosphatidylethanolamine-distearoylphosphatidyl...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.73.11.3862

    authors： Mabrey S,Sturtevant JM

    更新日期：1976-11-01 00:00:00

  • Power spectra reveal the influence of stochasticity on nonlinear population dynamics.

    abstract：:Stochasticity alters the nonlinear dynamics of inherently cycling populations. The power spectrum can describe and explain the impacts of stochasticity. We fitted models to short observed time series of flour beetle populations in the frequency domain, then used a well fitting stochastic mechanistic model to generate ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0608571103

    authors： Reuman DC,Desharnais RA,Costantino RF,Ahmad OS,Cohen JE

    更新日期：2006-12-05 00:00:00

  • Compressive fluorescence microscopy for biological and hyperspectral imaging.

    abstract：:The mathematical theory of compressed sensing (CS) asserts that one can acquire signals from measurements whose rate is much lower than the total bandwidth. Whereas the CS theory is now well developed, challenges concerning hardware implementations of CS-based acquisition devices--especially in optics--have only start...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1119511109

    authors： Studer V,Bobin J,Chahid M,Mousavi HS,Candes E,Dahan M

    更新日期：2012-06-26 00:00:00

  • Transcriptional map of 170-kb region at chromosome 11p15.5: identification and mutational analysis of the BWR1A gene reveals the presence of mutations in tumor samples.

    abstract：:Chromosome region 11p15.5 harbors unidentified genes involved in neoplasms and in the genetic disease Beckwith-Wiedemann syndrome. The genetic analysis of a 170-kb region at 11p15.5 between loci D11S601 and D11S679 resulted in the identification of six transcriptional units. Three genes, hNAP2, CDKN1C, and KVLQT1, are...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.7.3873

    authors： Schwienbacher C,Sabbioni S,Campi M,Veronese A,Bernardi G,Menegatti A,Hatada I,Mukai T,Ohashi H,Barbanti-Brodano G,Croce CM,Negrini M

    更新日期：1998-03-31 00:00:00

  • Mechanism of degradation of CPEB during Xenopus oocyte maturation.

    abstract：:CPEB, a cytoplasmic polyadenylation element-binding protein, plays an important role in translational control of maternal mRNAs in early animal development. During Xenopus oocyte maturation, CPEB undergoes a Cdc2-mediated phosphorylation- and ubiquitin-dependent degradation that is required for proper entry into meios...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0706952104

    authors： Setoyama D,Yamashita M,Sagata N

    更新日期：2007-11-13 00:00:00

  • Atypical nicotinic agonist bound conformations conferring subtype selectivity.

    abstract：:The nicotinic acetylcholine (ACh) receptor (nAChR) plays a crucial role in excitatory neurotransmission and is an important target for drugs and insecticides. Diverse nAChR subtypes with various subunit combinations confer differential selectivity for nicotinic drugs. We investigated the subtype selectivity of nAChR a...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0711724105

    authors： Tomizawa M,Maltby D,Talley TT,Durkin KA,Medzihradszky KF,Burlingame AL,Taylor P,Casida JE

    更新日期：2008-02-05 00:00:00

  • Global variability in seawater Mg:Ca and Sr:Ca ratios in the modern ocean.

    abstract：:Seawater Mg:Ca and Sr:Ca ratios are biogeochemical parameters reflecting the Earth-ocean-atmosphere dynamic exchange of elements. The ratios' dependence on the environment and organisms' biology facilitates their application in marine sciences. Here, we present a measured single-laboratory dataset, combined with previ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1918943117

    authors： Lebrato M,Garbe-Schönberg D,Müller MN,Blanco-Ameijeiras S,Feely RA,Lorenzoni L,Molinero JC,Bremer K,Jones DOB,Iglesias-Rodriguez D,Greeley D,Lamare MD,Paulmier A,Graco M,Cartes J,Barcelos E Ramos J,de Lara A,Sanchez-Lea

    更新日期：2020-09-08 00:00:00

  • Changes in hepatic messenger RNA for phosphoenolpyruvate carboxykinase (GTP) during development.

    abstract：:Phosphoenolpyruvate carboxykinase (GTP) [GTP;oxaloacetate carboxy-lyase(transphosphorylating); EC 4.1.1.32] is absent in rat liver cytosol during fetal life and is synthesized initially at birth. De novo synthesis of the enzyme can be induced prematurely by injection of dibutyryl cyclic AMP or glucagon into fetal anim...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.75.9.4189

    authors： Garcia Ruiz JP,Ingram R,Hanson RW

    更新日期：1978-09-01 00:00:00

  • MS3D structural elucidation of the HIV-1 packaging signal.

    abstract：:The structure of HIV-1 Psi-RNA has been elucidated by a concerted approach combining structural probes with mass spectrometric detection (MS3D), which is not affected by the size and crystallization properties of target biomolecules. Distance constraints from bifunctional cross-linkers provided the information require...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0800509105

    authors： Yu ET,Hawkins A,Eaton J,Fabris D

    更新日期：2008-08-26 00:00:00

  • NG-methyl-L-arginine causes endothelium-dependent contraction and inhibition of cyclic GMP formation in artery and vein.

    abstract：:The objective of this study was to determine whether the vascular smooth muscle contractile effect of NG-methyl-L-arginine (NMA) is endothelium dependent and attributed to a decline in smooth muscle levels of cyclic GMP. Vascular smooth muscle levels of cyclic GMP are severalfold greater in endothelium-intact than in ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.87.12.4430

    authors： Gold ME,Wood KS,Byrns RE,Fukuto J,Ignarro LJ

    更新日期：1990-06-01 00:00:00

  • An integral membrane component of coatomer-coated transport vesicles defines a family of proteins involved in budding.

    abstract：:We have isolated a major integral membrane protein from Golgi-derived coatomer-coated vesicles. This 24-kDa protein, p24, defines a family of integral membrane proteins with homologs present in yeast and humans. In addition to sequence similarity, all p24 family members contain a motif with the characteristic heptad r...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.17.8011

    authors： Stamnes MA,Craighead MW,Hoe MH,Lampen N,Geromanos S,Tempst P,Rothman JE

    更新日期：1995-08-15 00:00:00

  • Desensitization of enucleated cells to hormones and role of cytoskeleton in control of normal hormonal response.

    abstract：:Prostaglandin E1 and the beta-adrenergic hormone l-isoproterenol stimulated cyclic AMP formation in both nucleated and enucleated myeloid leukemic cells that could be induced to differentiate normally to mature cells by the macrophage- and granulocyte-inducing protein MGI (MGI+D+ cells). Enucleated as well as nucleate...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.77.8.4798

    authors： Simantov R,Shkolnik T,Sachs L

    更新日期：1980-08-01 00:00:00

  • Calculation of the three-dimensional structure of the membrane-bound portion of melittin from its amino acid sequence.

    abstract：:The structure of the NH2-terminal, 20-residue membrane-bound portion of melittin has been computed with empirical energies (ECEPP, Empirical Conformational Energy Program for Peptides). First, a search was made for long stretches of nonpolar residues. Then, the low-energy conformations of these segments were built up ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.79.16.5107

    authors： Pincus MR,Klausner RD,Scheraga HA

    更新日期：1982-08-01 00:00:00

  • Adaptive aneuploidy protects against thiol peroxidase deficiency by increasing respiration via key mitochondrial proteins.

    abstract：:Aerobic respiration is a fundamental energy-generating process; however, there is cost associated with living in an oxygen-rich environment, because partially reduced oxygen species can damage cellular components. Organisms evolved enzymes that alleviate this damage and protect the intracellular milieu, most notably t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1505315112

    authors： Kaya A,Gerashchenko MV,Seim I,Labarre J,Toledano MB,Gladyshev VN

    更新日期：2015-08-25 00:00:00

  • Insect feeding mobilizes a unique plant defense protease that disrupts the peritrophic matrix of caterpillars.

    abstract：:Plants frequently respond to herbivorous insect attack by synthesizing defense proteins that deter insect feeding and prevent additional herbivory. Maize (Zea mays L.) lines, resistant to feeding by a number of lepidopteran species, rapidly mobilize a unique 33-kDa cysteine protease in response to caterpillar feeding....

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.202224899

    authors： Pechan T,Cohen A,Williams WP,Luthe DS

    更新日期：2002-10-01 00:00:00

  • Studies on peptide:N-glycanase-p97 interaction suggest that p97 phosphorylation modulates endoplasmic reticulum-associated degradation.

    abstract：:During endoplasmic reticulum-associated degradation, the multifunctional AAA ATPase p97 is part of a protein degradation complex. p97 associates via its N-terminal domain with various cofactors to recruit ubiquitinated substrates. It also interacts with alternative substrate-processing cofactors, such as Ufd2, Ufd3, a...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0702966104

    authors： Zhao G,Zhou X,Wang L,Li G,Schindelin H,Lennarz WJ

    更新日期：2007-05-22 00:00:00

  • Radical mechanisms for 1,5-dihydroflavin reduction of carbonyl compounds.

    abstract：:A discussion of the expected mechanism of radical reduction of carbonyl compounds by dihydroflavin as it relates to the structure of the carbonyl compound is provided. Factors which must be taken into account are the free energies of formation of the radical anions (CR2O), THE ACIDITY OF THE CONJUGATE ACID OF THE CARB...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.72.5.1763

    authors： Williams RF,Shinkai S,Bruice TC

    更新日期：1975-05-01 00:00:00

  • Uncouplers and the molecular mechanism of uncoupling in mitochondria.

    abstract：:Uncouplers are molecules with protonophoric and ionophoric capabilities that mediate coupled cyclical transport of cations--a transport that takes precedence over all other coupled processes. Uncouplers form cation-containing complexes with electrogenic ionophores that potentiate cyclical transport of cations. The mol...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.74.6.2241

    authors： Kessler RJ,Vande Zande H,Tyson CA,Blondin GA,Fairfield J,Glasser P,Green DE

    更新日期：1977-06-01 00:00:00

  • Entner-Doudoroff pathway for sulfoquinovose degradation in Pseudomonas putida SQ1.

    abstract：:Sulfoquinovose (SQ; 6-deoxy-6-sulfoglucose) is the polar head group of the plant sulfolipid SQ-diacylglycerol, and SQ comprises a major proportion of the organosulfur in nature, where it is degraded by bacteria. A first degradation pathway for SQ has been demonstrated recently, a "sulfoglycolytic" pathway, in addition...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1507049112

    authors： Felux AK,Spiteller D,Klebensberger J,Schleheck D

    更新日期：2015-08-04 00:00:00

  • Probing chelation motifs in HIV integrase inhibitors.

    abstract：:A series of HIV integrase (HIV-1 IN) inhibitors were synthesized to evaluate the role of the metal-binding group (MBG) in this class of metalloenzyme inhibitors. A total of 21 different raltegravir-chelator derivative (RCD) compounds were prepared that differed only in the nature of the MBG. These IN strand-transfer i...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1112389109

    authors： Agrawal A,DeSoto J,Fullagar JL,Maddali K,Rostami S,Richman DD,Pommier Y,Cohen SM

    更新日期：2012-02-14 00:00:00

  • Ciliary contact interactions dominate surface scattering of swimming eukaryotes.

    abstract：:Interactions between swimming cells and surfaces are essential to many microbiological processes, from bacterial biofilm formation to human fertilization. However, despite their fundamental importance, relatively little is known about the physical mechanisms that govern the scattering of flagellated or ciliated cells ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1210548110

    authors： Kantsler V,Dunkel J,Polin M,Goldstein RE

    更新日期：2013-01-22 00:00:00

  • Androgens regulate the permeability of the blood-testis barrier.

    abstract：:Within the mammalian testis, specialized tight junctions between somatic Sertoli cells create basal and apical polarity within the cells, restrict movement of molecules between cells, and separate the seminiferous epithelium into basal and adluminal compartments. These tight junctions form the basis of the blood-testi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0506084102

    authors： Meng J,Holdcraft RW,Shima JE,Griswold MD,Braun RE

    更新日期：2005-11-15 00:00:00

  • Lyophilization-induced reversible changes in the secondary structure of proteins.

    abstract：:Changes in the secondary structure of some dozen different proteins upon lyophilization of their aqueous solutions have been investigated by means of Fourier-transform infrared spectroscopy in the amide III band region. Dehydration markedly (but reversibly) alters the secondary structure of all the proteins studied, a...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.24.10969

    authors： Griebenow K,Klibanov AM

    更新日期：1995-11-21 00:00:00

  • MLV glycosylated-Gag is an infectivity factor that rescues Nef-deficient HIV-1.

    abstract：:Optimal infectivity of HIV-1 virions requires synthesis of the HIV-1 regulatory protein Nef in some producer cells but not others. A survey of 18 lymphoid cell lines found that Nef was dispensable in three, each of which harbored gammaretroviruses. Nef-dependent cell lines were rendered Nef-independent by a cell-free ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1001554107

    authors： Pizzato M

    更新日期：2010-05-18 00:00:00

  • Persistence of deeply sourced iron in the Pacific Ocean.

    abstract：:Biological carbon fixation is limited by the supply of Fe in vast regions of the global ocean. Dissolved Fe in seawater is primarily sourced from continental mineral dust, submarine hydrothermalism, and sediment dissolution along continental margins. However, the relative contributions of these three sources to the Fe...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1420188112

    authors： Horner TJ,Williams HM,Hein JR,Saito MA,Burton KW,Halliday AN,Nielsen SG

    更新日期：2015-02-03 00:00:00

  • Intracellular disruption of rat heart lysosomes by leucine methyl ester: effects on protein degradation.

    abstract：:Perfusion of rat hearts with Krebs--Henseleit medium containing 10 mM L-leucine methyl ester leads to swelling of lysosomes and loss of lysosomal integrity within 30-60 min. No morphological changes can be detected in the nuclei, mitochondria, sarcoplasmic reticulum, or Golgi complex as a result of the treatment with ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.78.7.4426

    authors： Reeves JP,Decker RS,Crie JS,Wildenthal K

    更新日期：1981-07-01 00:00:00