Abstract:
:Ig class-switch recombination (CSR) is directed by the long and repetitive switch regions and requires activation-induced cytidine deaminase (AID). One of the conserved switch-region sequence motifs (AGCT) is a preferred site for AID-mediated DNA-cytosine deamination. By using somatic gene targeting and recombinase-mediated cassette exchange, we established a cell line-based CSR assay that allows manipulation of switch sequences at the endogenous locus. We show that AGCT is only one of a family of four WGCW motifs in the switch region that can facilitate CSR. We go on to show that it is the overlap of AID hotspots at WGCW sites on the top and bottom strands that is critical. This finding leads to a much clearer model for the difference between CSR and somatic hypermutation.
journal_name
Proc Natl Acad Sci U S Aauthors
Han L,Masani S,Yu Kdoi
10.1073/pnas.1018726108subject
Has Abstractpub_date
2011-07-12 00:00:00pages
11584-9issue
28eissn
0027-8424issn
1091-6490pii
1018726108journal_volume
108pub_type
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