Dissecting Fas signaling with an altered-specificity death-domain mutant: requirement of FADD binding for apoptosis but not Jun N-terminal kinase activation.

abstract：:L-Gulono-gamma-lactone oxidase (L-gulono-gamma-lactone:oxygen 2-oxidoreductase, EC 1.1.3.8) is the enzyme that catalyzes the terminal step of L-ascorbic acid biosynthesis in mammalian liver. The absence of the oxidase activity in primates and guinea pigs is the reason why these animals are subject to scurvy, which mus...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nishikimi M,Udenfriend S

更新日期：1976-06-01 00:00:00

abstract：:Understanding mammalian spermatozoan development and the events surrounding fertilization has grown slowly, in part because of uncertainty about the number and identity of the cellular components involved. Determination of those transcripts expressed specifically by germ cells should provide an inclusive list of proba...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schultz N,Hamra FK,Garbers DL

更新日期：2003-10-14 00:00:00

abstract：:Genetic potentials (pedigree-estimated breeding value) for milk and for fat were compared in cows grouped according to subclinical stage of bovine leukemia virus infection. Genetic potential for milk production was significantly greater in seropositive cows with persistent lymphocytosis (622 +/- 72 kg) and in seroposi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu MC,Shanks RD,Lewin HA

更新日期：1989-02-01 00:00:00

abstract：:Documented cases of convergent molecular evolution due to selection are fairly unusual, and examples to date have involved only a few amino acid positions. However, because convergence mimics shared ancestry and is not accommodated by current phylogenetic methods, it can strongly mislead phylogenetic inference when it...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Castoe TA,de Koning AP,Kim HM,Gu W,Noonan BP,Naylor G,Jiang ZJ,Parkinson CL,Pollock DD

更新日期：2009-06-02 00:00:00

abstract：:The molecular size and stability of the nerve growth factor (NGF) secreted in culture by L cells have been studied by sedimentation and gel filtration chromatography. Results indicate that L cell NGF has a molecular weight of 160,000. It contains as part of its structure a protein component that is biologically, immun...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pantazis NJ,Blanchard MH,Arnason BG,Young M

更新日期：1977-04-01 00:00:00

abstract：:Global increases in population, consumption, and gross domestic product raise concerns about the sustainability of the current and future use of natural resources. The human appropriation of net primary production (HANPP) provides a useful measure of human intervention into the biosphere. The productive capacity of la...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Krausmann F,Erb KH,Gingrich S,Haberl H,Bondeau A,Gaube V,Lauk C,Plutzar C,Searchinger TD

更新日期：2013-06-18 00:00:00

abstract：:How truth wins in social groups is an important open problem. Classic experiments on social groups dealing with truth statement issues present mixed findings on the conditions of truth abandonment and reaching a consensus on the truth. No theory has been developed and evaluated that might integrate these findings with...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Friedkin NE,Bullo F

更新日期：2017-10-24 00:00:00

abstract：:Using single-cell sequence analysis, we discovered that a high proportion of cells in tissues as diverse as buccal epithelium and heart muscle contain high proportions of clonal mutant mtDNA expanded from single initial mutant mtDNA molecules. We demonstrate that intracellular clonal expansion of somatic point mutatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nekhaeva E,Bodyak ND,Kraytsberg Y,McGrath SB,Van Orsouw NJ,Pluzhnikov A,Wei JY,Vijg J,Khrapko K

更新日期：2002-04-16 00:00:00

abstract：:Although advances in microfluidic technology have enabled increasingly sophisticated biosensing and bioassay operations to be performed at the microscale, many of these applications employ such small amounts of charged biomolecules (DNA, proteins, and peptides) that they must first be preconcentrated to a detectable l...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shaikh FA,Ugaz VM

更新日期：2006-03-28 00:00:00

abstract：:The properties of inositol 1,4,5-trisphosphate (InsP3) receptor sites in the anterior pituitary were evaluated by binding studies with InsP3 labeled with 32P to high specific radioactivity. Specific binding of Ins[32P]P3 was demonstrable in pituitary membrane preparations and was linearly proportional to the amount of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guillemette G,Balla T,Baukal AJ,Catt KJ

更新日期：1987-12-01 00:00:00

abstract：:In nearly all eukaryotes, at least some individuals inherit mitochondrial and chloroplast genes from only one parent. There is no single mechanism of uniparental inheritance: organelle gene inheritance is blocked by a variety of mechanisms and at different stages of reproduction in different species. Frequent changes ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Birky CW Jr

更新日期：1995-12-05 00:00:00

abstract：:Tissue- and cell-specific deletion of the Aqp4 gene is required to differentiate between the numerous pools of aquaporin-4 (AQP4) water channels. A glial-conditional Aqp4 knockout mouse line was generated to resolve whether astroglial AQP4 controls water exchange across the blood-brain interface. The conditional knock...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haj-Yasein NN,Vindedal GF,Eilert-Olsen M,Gundersen GA,Skare Ø,Laake P,Klungland A,Thorén AE,Burkhardt JM,Ottersen OP,Nagelhus EA

更新日期：2011-10-25 00:00:00

abstract：:Actin polymerization and assembly into stress fibers (SFs) is central to many cellular processes. However, how SFs form in response to the mechanical interaction of cells with their environment is not fully understood. Here we have identified Piezo2 mechanosensitive cationic channel as a transducer of environmental ph...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pardo-Pastor C,Rubio-Moscardo F,Vogel-González M,Serra SA,Afthinos A,Mrkonjic S,Destaing O,Abenza JF,Fernández-Fernández JM,Trepat X,Albiges-Rizo C,Konstantopoulos K,Valverde MA

更新日期：2018-02-20 00:00:00

abstract：:Retinal prosthetics offer hope for patients with retinal degenerative diseases. There are 20-25 million people worldwide who are blind or facing blindness due to these diseases, and they have few treatment options. Drug therapies are able to help a small fraction of the population, but for the vast majority, their bes...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nirenberg S,Pandarinath C

更新日期：2012-09-11 00:00:00

abstract：:Proper regulation of the intracellular ion concentration is essential to maintain life and is achieved by ion transporters that transport their substrates across the membrane in a strictly regulated manner. MgtE is a Mg(2+) transporter that may function in the homeostasis of the intracellular Mg(2+) concentration. A r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ishitani R,Sugita Y,Dohmae N,Furuya N,Hattori M,Nureki O

更新日期：2008-10-07 00:00:00

abstract：:Xanthophyll pigments have critical structural and functional roles in the photosynthetic light-harvesting complexes of algae and vascular plants. Genetic dissection of xanthophyll metabolism in the green alga Chlamydomonas reinhardtii revealed functions for specific xanthophylls in the nonradiative dissipation of exce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Niyogi KK,Björkman O,Grossman AR

更新日期：1997-12-09 00:00:00

abstract：:Loss of a whole chromosome 5 or a deletion of its long arm (5q) is a recurring abnormality in malignant myeloid neoplasms. To determine the location of genes on 5q that may be involved in leukemogenesis, we examined the deleted chromosome 5 homologs in a series of 135 patients with malignant myeloid diseases. By compa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Le Beau MM,Espinosa R 3rd,Neuman WL,Stock W,Roulston D,Larson RA,Keinanen M,Westbrook CA

更新日期：1993-06-15 00:00:00

abstract：:We demonstrate how the wavelet transform, which is a powerful tool for compression, filtering, and scaling analysis of signals, may be used to separate large- and short-scale electron density features in X-ray diffraction patterns. Wavelets can isolate the electron density associated with delocalized bonds from the mu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Osipov VA,Kowalewski M,Mukamel S

更新日期：2018-10-09 00:00:00

abstract：:Stromal cell lines were established from canine long-term marrow cultures, cloned by limiting dilution, and maintained in stromal cell-conditioned medium. These cells grew adherent, maintained stable growth rate and morphology under standard conditions (in 20-30% conditioned medium; confluency, 70-90%), and supported ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huss R,Hong DS,McSweeney PA,Hoy CA,Deeg HJ

更新日期：1995-01-31 00:00:00

abstract：:Based largely on studies in xenograft models, lipocalin-2 (Lcn2) has been implicated in the progression of multiple types of human tumors, including breast cancer. Here we examine the role of Lcn2 in mammary tumorigenesis and lung metastasis using an in vivo molecular genetics approach. We crossed a well-characterized...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berger T,Cheung CC,Elia AJ,Mak TW

更新日期：2010-02-16 00:00:00

abstract：:We report the heavy chain variable region sequences from the cDNAs of five previously described monoclonal cell lines producing human antibodies specific for the human immunodeficiency virus type 1 and detail the molecular characteristics, germ-line origins, and extent of somatic mutation among these antibodies. Three...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Andris JS,Johnson S,Zolla-Pazner S,Capra JD

更新日期：1991-09-01 00:00:00

abstract：:HLA-I molecules play a central role in antigen presentation. They typically bind 9- to 12-mer peptides, and their canonical binding mode involves anchor residues at the second and last positions of their ligands. To investigate potential noncanonical binding modes, we collected in-depth and accurate HLA peptidomics da...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guillaume P,Picaud S,Baumgaertner P,Montandon N,Schmidt J,Speiser DE,Coukos G,Bassani-Sternberg M,Filippakopoulos P,Gfeller D

更新日期：2018-05-15 00:00:00

abstract：:We previously demonstrated that platelets express high levels of the tyrosine protein kinase pp60c-src. By a quantitative immunoblot assay, it is shown in this report that pp60c-src represents 0.2-0.4% of total platelet protein. The expression of high levels of pp60c-src in platelets correlated with high levels of tot...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Golden A,Brugge JS

更新日期：1989-02-01 00:00:00

abstract：:After exposure to low density lipoprotein (LDL) that had been minimally modified by oxidation (MM-LDL), human endothelial cells (EC) and smooth muscle cells (SMC) cultured separately or together produced 2- to 3-fold more monocyte chemotactic activity than did control cells or cells exposed to freshly isolated LDL. Th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cushing SD,Berliner JA,Valente AJ,Territo MC,Navab M,Parhami F,Gerrity R,Schwartz CJ,Fogelman AM

更新日期：1990-07-01 00:00:00

abstract：:The synthesis of cytoplasmic proteins from quiescent and serum-stimulated Swiss 3T3 cells was compared by two-dimensional polyacrylamide gel electrophoresis. Four new proteins of Mrs 26,000, 28,000, 45,000, and 47,000 designated N26, N28, N45, and N47, which were not detectable in quiescent cells, appeared 60 min afte...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thomas G,Thomas G,Luther H

更新日期：1981-09-01 00:00:00

abstract：:We have screened a human DNA library using as probe a chimpanzee sequence that contains homology to the polymerase gene of the endogenous baboon virus. One set of overlapping clones spans about 20 kilobases and contains regions of DNA sequence homology to the gag p30, gag p15, and polymerase genes of Moloney murine le...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bonner TI,O'Connell C,Cohen M

更新日期：1982-08-01 00:00:00

abstract：:Regulated intramembrane proteolysis by gamma-secretase cleaves proteins in their transmembrane domain and is involved in important signaling pathways. At least four different gamma-secretase complexes have been identified, but little is known about their biological role and specificity. Previous work has demonstrated ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dejaegere T,Serneels L,Schäfer MK,Van Biervliet J,Horré K,Depboylu C,Alvarez-Fischer D,Herreman A,Willem M,Haass C,Höglinger GU,D'Hooge R,De Strooper B

更新日期：2008-07-15 00:00:00

abstract：:The phosphatase and tensin homolog (PTEN) is a tumor suppressor that is inactivated in many human cancers. PTEN loss has been associated with resistance to inhibitors of the epidermal growth factor receptor (EGFR), but the molecular basis of this resistance is unclear. It is believed that unopposed phosphatidylinosito...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vivanco I,Rohle D,Versele M,Iwanami A,Kuga D,Oldrini B,Tanaka K,Dang J,Kubek S,Palaskas N,Hsueh T,Evans M,Mulholland D,Wolle D,Rajasekaran S,Rajasekaran A,Liau LM,Cloughesy TF,Dikic I,Brennan C,Wu H,Mischel PS,

更新日期：2010-04-06 00:00:00

abstract：:Most B lymphocytes in normal individuals express two classes of cell-surface immunoglobulins, IgM and IgD. The specificity of the two antigen receptors is identical since they are produced by transcription and differential splicing of the same variable region gene segment to the heavy-chain constant region gene segmen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Irsch J,Irlenbusch S,Radl J,Burrows PD,Cooper MD,Radbruch AH

更新日期：1994-02-15 00:00:00

abstract：:The proton-translocating subunit of wheat chloroplast ATP synthase is encoded by a chloroplast gene that has been accurately mapped and whose nucleotide sequence has been determined. The predicted sequence of 81 amino acids has been confirmed in part by determination of the sequence of the first 40 amino acids from th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Howe CJ,Auffret AD,Doherty A,Bowman CM,Dyer TA,Gray JC

更新日期：1982-11-01 00:00:00