当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Designing a functional type 2 copper center that has nitrite reductase activity within α-helical coiled coils.

Designing a functional type 2 copper center that has nitrite reductase activity within α-helical coiled coils.

Abstract：

:One of the ultimate objectives of de novo protein design is to realize systems capable of catalyzing redox reactions on substrates. This goal is challenging as redox-active proteins require design considerations for both the reduced and oxidized states of the protein. In this paper, we describe the spectroscopic characterization and catalytic activity of a de novo designed metallopeptide Cu(I/II)(TRIL23H)(3)(+/2+), where Cu(I/II) is embeded in α-helical coiled coils, as a model for the Cu(T2) center of copper nitrite reductase. In Cu(I/II)(TRIL23H)(3)(+/2+), Cu(I) is coordinated to three histidines, as indicated by X-ray absorption data, and Cu(II) to three histidines and one or two water molecules. Both ions are bound in the interior of the three-stranded coiled coils with affinities that range from nano- to micromolar [Cu(II)], and picomolar [Cu(I)]. The Cu(His)(3) active site is characterized in both oxidation states, revealing similarities to the Cu(T2) site in the natural enzyme. The species Cu(II)(TRIL23H)(3)(2+) in aqueous solution can be reduced to Cu(I)(TRIL23H)(3)(+) using ascorbate, and reoxidized by nitrite with production of nitric oxide. At pH 5.8, with an excess of both the reductant (ascorbate) and the substrate (nitrite), the copper peptide Cu(II)(TRIL23H)(3)(2+) acts as a catalyst for the reduction of nitrite with at least five turnovers and no loss of catalytic efficiency after 3.7 h. The catalytic activity, which is first order in the concentration of the peptide, also shows a pH dependence that is described and discussed.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Tegoni M,Yu F,Bersellini M,Penner-Hahn JE,Pecoraro VL

doi

10.1073/pnas.1212893110

subject

Has Abstract

pub_date

2012-12-26 00:00:00

pages

21234-9

issue

52

eissn

0027-8424

issn

1091-6490

pii

1212893110

journal_volume

109

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Diversity of gene expression in adenocarcinoma of the lung.

    abstract：:The global gene expression profiles for 67 human lung tumors representing 56 patients were examined by using 24,000-element cDNA microarrays. Subdivision of the tumors based on gene expression patterns faithfully recapitulated morphological classification of the tumors into squamous, large cell, small cell, and adenoc...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.241500798

    authors： Garber ME,Troyanskaya OG,Schluens K,Petersen S,Thaesler Z,Pacyna-Gengelbach M,van de Rijn M,Rosen GD,Perou CM,Whyte RI,Altman RB,Brown PO,Botstein D,Petersen I

    更新日期：2001-11-20 00:00:00

  • Microhomology-mediated and nonhomologous repair of a double-strand break in the chloroplast genome of Arabidopsis.

    abstract：:Chloroplast DNA (cpDNA) is under great photooxidative stress, yet its evolution is very conservative compared with nuclear or mitochondrial genomes. It can be expected that DNA repair mechanisms play important roles in cpDNA survival and evolution, but they are poorly understood. To gain insight into how the most seve...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1004326107

    authors： Kwon T,Huq E,Herrin DL

    更新日期：2010-08-03 00:00:00

  • Functional, fractal nonlinear response with application to rate processes with memory, allometry, and population genetics.

    abstract：:We give a functional generalization of fractal scaling laws applied to response problems as well as to probability distributions. We consider excitations and responses, which are functions of a given state vector. Based on scaling arguments, we derive a general nonlinear response functional scaling law, which expresse...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0700397104

    authors： Vlad MO,Morán F,Popa VT,Szedlacsek SE,Ross J

    更新日期：2007-03-20 00:00:00

  • Accumulation of formamide in hydrothermal pores to form prebiotic nucleobases.

    abstract：:Formamide is one of the important compounds from which prebiotic molecules can be synthesized, provided that its concentration is sufficiently high. For nucleotides and short DNA strands, it has been shown that a high degree of accumulation in hydrothermal pores occurs, so that temperature gradients might play a role ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1600275113

    authors： Niether D,Afanasenkau D,Dhont JK,Wiegand S

    更新日期：2016-04-19 00:00:00

  • Pivotal role of amino acid at position 138 in the allosteric hinge reorientation of cAMP receptor protein.

    abstract：:The cAMP receptor protein (CRP) of Escherichia coli needs cAMP for an allosteric change to regulate gene expression by binding to specific DNA sites. The hinge region connecting the DNA-binding domain to the cAMP-binding domain has been proposed to participate in the cAMP-induced allosteric change necessary to adjust ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.90.1.75

    authors： Ryu S,Kim J,Adhya S,Garges S

    更新日期：1993-01-01 00:00:00

  • Analysis of the mechanism, rate, and sites of proteolytic cleavage of human immunoglobulin D by high-pressure liquid chromatography.

    abstract：:The high susceptibility of human immunoglobulin D to proteolytic degradation affects its biological function, metabolism, and immunoassay. High-pressure liquid chromatography was used to investigate the mechanism and rate of limited proteolytic cleavage of IgD and also to identify, isolate, and quantify the reaction p...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.1.61

    authors： Ishioka N,Takahashi N,Putnam FW

    更新日期：1987-01-01 00:00:00

  • Simultaneous fluorescence-activated cell sorter analysis of two distinct transcriptional elements within a single cell using engineered green fluorescent proteins.

    abstract：:Green fluorescent protein (GFP) is widely used as a reporter gene in both prokaryotes and eukaryotes. However, the fluorescence levels of wild-type GFP (wtGFP) are not bright enough for fluorescence-activated cell sorting or flow cytometry. Several GFP variants were generated that are brighter or have altered excitati...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.16.8508

    authors： Anderson MT,Tjioe IM,Lorincz MC,Parks DR,Herzenberg LA,Nolan GP,Herzenberg LA

    更新日期：1996-08-06 00:00:00

  • Expression of murine 21-hydroxylase in mouse adrenal glands and in transfected Y1 adrenocortical tumor cells.

    abstract：:The S region of the murine major histocompatibility complex contains two structurally related genes (21-OHase A and 21-OHase B) that encode 21-hydroxylase (21-OHase), an enzyme essential for the synthesis of adrenal steroids. Expression of these two genes has been analyzed by using oligonucleotide probes specific for ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.82.23.7860

    authors： Parker KL,Chaplin DD,Wong M,Seidman JG,Smith JA,Schimmer BP

    更新日期：1985-12-01 00:00:00

  • Pregrowth hormone messenger RNA: glucocorticoid induction and identification in rat pituitary cells.

    abstract：:We have investigated whether the large (5- to 15-fold) stimulation of synthesis of growth hormone (somatotropin) observed upon exposure of a strain of rat pituitary (GH3) cells to dexamethasone (a synthetic glucocorticoid) arises from a stimulation of pregrowth hormone mRNA. A good agreement was observed between the s...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.74.6.2357

    authors： Tushinski RJ,Sussman PM,Yu LY,Bancroft FC

    更新日期：1977-06-01 00:00:00

  • Control of cell membrane tension by myosin-I.

    abstract：:All cell functions that involve membrane deformation or a change in cell shape (e.g., endocytosis, exocytosis, cell motility, and cytokinesis) are regulated by membrane tension. While molecular contacts between the plasma membrane and the underlying actin cytoskeleton are known to make significant contributions to mem...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0901641106

    authors： Nambiar R,McConnell RE,Tyska MJ

    更新日期：2009-07-21 00:00:00

  • Dengue reporter viruses reveal viral dynamics in interferon receptor-deficient mice and sensitivity to interferon effectors in vitro.

    abstract：:Dengue virus (DENV) is a global disease threat for which there are no approved antivirals or vaccines. Establishing state-of-the-art screening systems that rely on fluorescent or luminescent reporters may accelerate the development of anti-DENV therapeutics. However, relatively few reporter DENV platforms exist. Here,...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1212379109

    authors： Schoggins JW,Dorner M,Feulner M,Imanaka N,Murphy MY,Ploss A,Rice CM

    更新日期：2012-09-04 00:00:00

  • Molecular characterization of five human anti-human immunodeficiency virus type 1 antibody heavy chains reveals extensive somatic mutation typical of an antigen-driven immune response.

    abstract：:We report the heavy chain variable region sequences from the cDNAs of five previously described monoclonal cell lines producing human antibodies specific for the human immunodeficiency virus type 1 and detail the molecular characteristics, germ-line origins, and extent of somatic mutation among these antibodies. Three...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.88.17.7783

    authors： Andris JS,Johnson S,Zolla-Pazner S,Capra JD

    更新日期：1991-09-01 00:00:00

  • Foxg1 localizes to mitochondria and coordinates cell differentiation and bioenergetics.

    abstract：:Forkhead box g1 (Foxg1) is a nuclear-cytosolic transcription factor essential for the forebrain development and involved in neurodevelopmental and cancer pathologies. Despite the importance of this protein, little is known about the modalities by which it exerts such a large number of cellular functions. Here we show ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1515190112

    authors： Pancrazi L,Di Benedetto G,Colombaioni L,Della Sala G,Testa G,Olimpico F,Reyes A,Zeviani M,Pozzan T,Costa M

    更新日期：2015-11-10 00:00:00

  • Steady-state electrophoresis: a technique for measuring physical properties of macro-ions.

    abstract：:The basic theory of a newly discovered physical technique, steady-state electrophoresis, is described followed by analyses of several representative macro-ion systems with the technique. This method measures apparent moments of molar diminished charge (defined below) of solvated macro-ions from an analysis of a concen...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.86.12.4479

    authors： Godfrey JE

    更新日期：1989-06-01 00:00:00

  • Atypical nicotinic agonist bound conformations conferring subtype selectivity.

    abstract：:The nicotinic acetylcholine (ACh) receptor (nAChR) plays a crucial role in excitatory neurotransmission and is an important target for drugs and insecticides. Diverse nAChR subtypes with various subunit combinations confer differential selectivity for nicotinic drugs. We investigated the subtype selectivity of nAChR a...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0711724105

    authors： Tomizawa M,Maltby D,Talley TT,Durkin KA,Medzihradszky KF,Burlingame AL,Taylor P,Casida JE

    更新日期：2008-02-05 00:00:00

  • Vasoactive intestinal peptide induces regulatory dendritic cells with therapeutic effects on autoimmune disorders.

    abstract：:The induction of antigen-specific tolerance is critical for the prevention of autoimmunity and maintenance of immune tolerance. In addition to their classical role as sentinels of the immune response-inducing T cell reactivity, dendritic cells (DCs) play an important role in maintaining peripheral tolerance through th...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0504484102

    authors： Chorny A,Gonzalez-Rey E,Fernandez-Martin A,Pozo D,Ganea D,Delgado M

    更新日期：2005-09-20 00:00:00

  • Components of fitness become effectively neutral in equilibrium populations.

    abstract：:If, because of trade-offs or similar considerations, components of fitness are negatively correlated in equilibrium populations, they may then exhibit no correlation with total fitness. Lack of correlation with fitness is a fundamental characteristic of selective neutrality. Hence, selectively important traits become ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.87.5.2018

    authors： Reeve R,Smith E,Wallace B

    更新日期：1990-03-01 00:00:00

  • Inhibitor-binding mode of homobelactosin C to proteasomes: new insights into class I MHC ligand generation.

    abstract：:Most class I MHC ligands are generated from the vast majority of cellular proteins by proteolysis within the ubiquitin-proteasome pathway and are presented on the cell surface by MHC class I molecules. Here, we present the crystallographic analysis of yeast 20S proteasome in complex with the inhibitor homobelactosin C...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0600647103

    authors： Groll M,Larionov OV,Huber R,de Meijere A

    更新日期：2006-03-21 00:00:00

  • Transport of the new chemotherapeutic agent beta-D-glucosylisophosphoramide mustard (D-19575) into tumor cells is mediated by the Na+-D-glucose cotransporter SAAT1.

    abstract：:For beta-D-glucosylisophosphoramide mustard (beta-D-Glc-IPM), a new alkylating drug in which isophosphoramide mustard is stabilized, a higher selectivity and lower myelotoxicity was observed than for the currently used cytostatic ifosfamide. Because beta-D-Glc-IPM is hydrophilic and does not diffuse passively through ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.6.2914

    authors： Veyhl M,Wagner K,Volk C,Gorboulev V,Baumgarten K,Weber WM,Schaper M,Bertram B,Wiessler M,Koepsell H

    更新日期：1998-03-17 00:00:00

  • The animal model of human amnesia: long-term memory impaired and short-term memory intact.

    abstract：:Normal monkeys and monkeys with lesions of the hippocampal formation and adjacent cortex (the H+ lesion) were trained on the delayed nonmatching to sample (DNMS) task with a delay of 0.5 s between the sample and the choice. The animals with H+ lesions learned the task normally at this short delay and also exhibited th...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.91.12.5637

    authors： Alvarez P,Zola-Morgan S,Squire LR

    更新日期：1994-06-07 00:00:00

  • Stimulation and inhibition of anti-hapten responses in guinea pigs immunized with hybrid liposomes.

    abstract：:Guinea pigs were immunized with liposomal model membranes containing phosphatidylethanolamine (PE) or glycerophosphorylethanolamine (GPE) derivatives in which the amino function was substituted with either dinitrophenylaminocaproyl (Dnp-Cap) or mono(p-azobenzenearsonic acid)tyrosyl (ABA-Tyr) residues. Previous studies...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.72.11.4582

    authors： Kochibe N,Nicolotti RA,Davie JM,Kinsky SC

    更新日期：1975-11-01 00:00:00

  • Triplet states in photosystem I of spinach chloroplasts and subchloroplast particles.

    abstract：:We report light-induced electron paramagnetic resonance triplet spectra from samples of chloroplasts or digitonin photosystem I particles that depend upon the dark redox state of the bound acceptors of photosystem I. If the reaction centers are prepared in the redox state P-700 A X- FdB-FdA-, then upon illumination at...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.76.10.5124

    authors： Frank HA,McLean MB,Sauer K

    更新日期：1979-10-01 00:00:00

  • Does vegetation prevent wave erosion of salt marsh edges?

    abstract：:This study challenges the paradigm that salt marsh plants prevent lateral wave-induced erosion along wetland edges by binding soil with live roots and clarifies the role of vegetation in protecting the coast. In both laboratory flume studies and controlled field experiments, we show that common salt marsh plants do no...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0901297106

    authors： Feagin RA,Lozada-Bernard SM,Ravens TM,Möller I,Yeager KM,Baird AH

    更新日期：2009-06-23 00:00:00

  • Netrin-1 induces angiogenesis via a DCC-dependent ERK1/2-eNOS feed-forward mechanism.

    abstract：:Netrin-1 is critical for axonal pathfinding which shares similarities with formation of vascular network. Here we report that netrin-1 induction of angiogenesis is mediated by an increase in endothelial nitric oxide (NO*) production, which occurs via a DCC-dependent, ERK1/2-eNOS feed-forward mechanism. Exposure of mat...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0511011103

    authors： Nguyen A,Cai H

    更新日期：2006-04-25 00:00:00

  • Acid catalysis of the formation of the slow-folding species of RNase A: evidence that the reaction is proline isomerization.

    abstract：:Unfolded RNase A is known to contain an equilibrium mixture of two forms, a slow-folding form (U(1)) and a fast-folding form (U(2)). If U(1) is produced after unfolding by the slow cis-trans isomerization of proline residues about X-Pro imide bonds, then the formation of U(1) should be catalyzed by strong acids. There...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.75.10.4764

    authors： Schmid FX,Baldwin RL

    更新日期：1978-10-01 00:00:00

  • Dissecting the role of the golgi complex and lipid rafts in biosynthetic transport of cholesterol to the cell surface.

    abstract：:In this study, we compared the transport of newly synthesized cholesterol with that of influenza virus hemagglutinin (HA) from the endoplasmic reticulum to the plasma membrane. The arrival of cholesterol on the cell surface was monitored by cyclodextrin removal, and HA transport was monitored by surface trypsinization...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.140218797

    authors： Heino S,Lusa S,Somerharju P,Ehnholm C,Olkkonen VM,Ikonen E

    更新日期：2000-07-18 00:00:00

  • Reporters of TCR signaling identify arthritogenic T cells in murine and human autoimmune arthritis.

    abstract：:How pathogenic cluster of differentiation 4 (CD4) T cells in rheumatoid arthritis (RA) develop remains poorly understood. We used Nur77-a marker of T cell antigen receptor (TCR) signaling-to identify antigen-activated CD4 T cells in the SKG mouse model of autoimmune arthritis and in patients with RA. Using a fluoresce...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1904271116

    authors： Ashouri JF,Hsu LY,Yu S,Rychkov D,Chen Y,Cheng DA,Sirota M,Hansen E,Lattanza L,Zikherman J,Weiss A

    更新日期：2019-09-10 00:00:00

  • The Na+/I symporter (NIS) mediates electroneutral active transport of the environmental pollutant perchlorate.

    abstract：:The Na(+)/I(-) symporter (NIS) is a key plasma membrane protein that mediates active I(-) uptake in the thyroid, lactating breast, and other tissues with an electrogenic stoichiometry of 2 Na(+) per I(-). In the thyroid, NIS-mediated I(-) uptake is the first step in the biosynthesis of the iodine-containing thyroid ho...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0707207104

    authors： Dohán O,Portulano C,Basquin C,Reyna-Neyra A,Amzel LM,Carrasco N

    更新日期：2007-12-18 00:00:00

  • Structure-guided enzymology of the lipid A acyltransferase LpxM reveals a dual activity mechanism.

    abstract：:Gram-negative bacteria possess a characteristic outer membrane, of which the lipid A constituent elicits a strong host immune response through the Toll-like receptor 4 complex, and acts as a component of the permeability barrier to prevent uptake of bactericidal compounds. Lipid A species comprise the bulk of the oute...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1610746113

    authors： Dovala D,Rath CM,Hu Q,Sawyer WS,Shia S,Elling RA,Knapp MS,Metzger LE 4th

    更新日期：2016-10-11 00:00:00

  • Oxalate-curcumin-based probe for micro- and macroimaging of reactive oxygen species in Alzheimer's disease.

    abstract：:Alzheimer's disease (AD) is an irreversible neurodegenerative disorder that has a progression that is closely associated with oxidative stress. It has long been speculated that the reactive oxygen species (ROS) level in AD brains is much higher than that in healthy brains. However, evidence from living beings is scarc...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1706248114

    authors： Yang J,Zhang X,Yuan P,Yang J,Xu Y,Grutzendler J,Shao Y,Moore A,Ran C

    更新日期：2017-11-21 00:00:00