当前位置： SCI文献检索 > DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY期刊下所有文献 > Nonspecific cytotoxic cells in fish (Ictalurus punctatus). I. Optimum requirements for target cell lysis.

Nonspecific cytotoxic cells in fish (Ictalurus punctatus). I. Optimum requirements for target cell lysis.

Abstract：

:Nonspecific cytotoxic cells (NCC) obtained from the head (anterior) kidney of fish (Ictalurus punctatus) lyse human transformed B-cell targets. Lysis depended on direct cell-cell contact. Fish size, age, environmental holding temperatures, and lytic reaction conditions such as osmolality and optimum effector:target cell ratios were optimized. Experiments to characterize optimum kinetics demonstrated highly efficient killing after two hours incubation. This rapid cytolysis was further studied by determining NCC activity against appropriately labeled target cells after 30, 60, 90 and 120 minutes of cocultivation. At 160:1 (E:T) greater than 40% of the 5 hour percent specific release value was produced after 30 minutes. After 90 minutes, more than 90% of total percent specific release was observed. At least one mechanism of regulation of NCC killing was described. In the presence of normal (homologous or heterologous) catfish serum (CFS), essentially no NCC activity was observed. This suppression was reversible by preincubation in 10% fetal bovine serum (FBS). NCC "activation" by preincubation in 10% FBS was time-dependent (at least four hours was required to generate significant lysis). NCC activation could be reversed by treating potentially lytic cells with supernatants containing dissociated CFS. In addition, reversible activation could be demonstrated by treating potentially lytic effector cells with CFS to produce suppression. Regulation occurred at the effector cell level because treated target cells did not suppress NCC activity. These data demonstrate a population of nonspecific effector cytolytic cells that potentially represent a phylogenetic precursor to mammalian natural killer cells.

journal_name

Dev Comp Immunol

journal_title

Developmental and comparative immunology

authors

Graves SS,Evans DL,Cobb D,Dawe DL

doi

10.1016/0145-305x(84)90036-3

subject

Has Abstract

pub_date

1984-04-01 00:00:00

pages

293-302

issue

2

eissn

0145-305X

issn

1879-0089

pii

0145-305X(84)90036-3

journal_volume

8

pub_type

杂志文章

相关文献

DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY文献大全
  • Developmental regulation of chicken surfactant protein A and its localization in lung.

    abstract：:Surfactant Protein A (SP-A) is a collagenous C-type lectin (collectin) that plays an important role in the early stage of the host immune response. In chicken, SP-A (cSP-A) is expressed as a 26 kDa glycosylated protein in the lung. Using immunohistochemistry, cSP-A protein was detected mainly in the lung lining fluid ...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2016.03.010

    authors： Zhang W,Cuperus T,van Dijk A,Skjødt K,Hansen S,Haagsman HP,Veldhuizen EJ

    更新日期：2016-08-01 00:00:00

  • Expression regulation of zebrafish interferon regulatory factor 9 by promoter analysis.

    abstract：:We previously showed that a fish interferon (IFN) regulatory factor 9 (IRF9) homologue, crucian carp Carassius auratus IRF9, displays constitutively nuclear localization and involvement in fish IFN-dependent JAK-STAT signaling; however, little is known about the expression regulation of fish IRF9. Here, we characteriz...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2013.07.017

    authors： Shi J,Zhang YB,Zhang JS,Gui JF

    更新日期：2013-12-01 00:00:00

  • Molecular characterization, phylogeny and expression of a hepcidin gene in the blotched snakehead Channa maculata.

    abstract：:A hepcidin-like gene (cmHep) was cloned and characterized from the liver of the blotched snakehead Channa maculata. The complete cmHep cDNA was 756 bp in length, containing an open reading frame of 270 bp (encoding 89 amino acids), flanked by 210 bp and 276 bp of 5' and 3' untranslated regions, respectively. The deduc...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2013.11.007

    authors： Gong LC,Wang H,Deng L

    更新日期：2014-05-01 00:00:00

  • Transcriptome analysis of hagfish leukocytes: a framework for understanding the immune system of jawless fishes.

    abstract：:Jawless fishes occupy a critical phylogenetic position in understanding the origin of the adaptive immune system. Here, we performed large-scale expressed sequence tag analysis of leukocytes isolated from the inshore hagfish Eptatretus burgeri. Although we found many immunity-related genes such as those involved in ly...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2004.04.005

    authors： Suzuki T,Shin-I T,Kohara Y,Kasahara M

    更新日期：2004-08-01 00:00:00

  • Chicken interferons, their receptors and interferon-stimulated genes.

    abstract：:The prevalence of pathogenic viruses is a serious issue as they pose a constant threat to both the poultry industry and to human health. To prevent these viral infections an understanding of the host-virus response is critical, especially for the development of novel therapeutics. One approach in the control of viral ...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章,评审

    doi：10.1016/j.dci.2013.05.020

    authors： Goossens KE,Ward AC,Lowenthal JW,Bean AG

    更新日期：2013-11-01 00:00:00

  • Chicken cathelicidins as potent intrinsically disordered biocides with antimicrobial activity against infectious pathogens.

    abstract：:This study was performed to identify the expression patterns of the cathelicidin genes in a local chicken breed and to evaluate the antimicrobial activities of the cathelicidin peptides against pathogenic bacteria. This analysis revealed that the coding regions of CATH-1, -2, and -3 genes contain 447 bp, 465 bp, and 4...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2016.06.012

    authors： Yacoub HA,Elazzazy AM,Mahmoud MM,Baeshen MN,Al-Maghrabi OA,Alkarim S,Ahmed ES,Almehdar HA,Uversky VN

    更新日期：2016-12-01 00:00:00

  • Biochemical characterization of evasion from peptidoglycan recognition by Staphylococcus aureus D-alanylated wall teichoic acid in insect innate immunity.

    abstract：:We recently reported that D-alanylation of Staphylococcus aureus wall teichoic acid (WTA) mitigates an induction of the Toll-mediated humoral response in Drosophila by interfering with peptidoglycan (PG) recognition by PG recognition protein-SA (PGRP-SA). Here, we investigated the mode of this interference by using an...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2011.03.001

    authors： Kurokawa K,Gong JH,Ryu KH,Zheng L,Chae JH,Kim MS,Lee BL

    更新日期：2011-08-01 00:00:00

  • Overexpression of NF-κB inhibitor alpha in Cynoglossus semilaevis impairs pathogen-induced immune response.

    abstract：:IκBα is a member of the NF-κB inhibitor family that inhibits NF-κB activity by sequestering NF-κB in an inactive form in the cytosol. Unlike mammalian IκBα, which has been extensively studied, very little is known about the function of fish IκBα. In this study, we identified and analyzed an IκBα homologue, CsIκBα from...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2011.08.001

    authors： Zhang M,Xiao ZZ,Sun L

    更新日期：2012-01-01 00:00:00

  • Characterization of thioredoxin-like PROTEIN-5 (TRXLP-5) and its differential response to grafting challenge in the black coloured selected line and control stocks of Pinctada fucata martensii.

    abstract：:Thioredoxin-like protein 5 (Trxlp-5) is a thioredoxin isoform associated with cellular redox homeostasis through the activity of thiol-disulfide reductase. In our study, Trxlp-5 was identified and characterized in Pinctada fucata martensii. The expression of PmTrxlp-5 was detected in response to polyinosinic: polycyti...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2020.103635

    authors： Adzigbli L,Zheng Z,Liao Y,Deng Y,Du X,Yang C

    更新日期：2020-05-01 00:00:00

  • High sequence variability among hemocyte-specific Kazal-type proteinase inhibitors in decapod crustaceans.

    abstract：:Crustacean hemocytes were found to produce a large number of transcripts coding for Kazal-type proteinase inhibitors (KPIs). A detailed study performed with the crayfish Pacifastacus leniusculus and the shrimp Penaeus monodon revealed the presence of at least 26 and 20 different Kazal domains from the hemocyte KPIs, r...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2009.08.005

    authors： Cerenius L,Liu H,Zhang Y,Rimphanitchayakit V,Tassanakajon A,Gunnar Andersson M,Söderhäll K,Söderhäll I

    更新日期：2010-01-01 00:00:00

  • In silico analysis of Schmidtea mediterranea TIR domain-containing proteins.

    abstract：:While genetic evidence points towards an absence of Toll-Like Receptors (TLRs) in Platyhelminthes, the Toll/IL-1 Receptor (TIR)-domains that drive the assembly of signalling complexes downstream TLR are present in these organisms. Here, we undertook the characterisation of the repertoire of TIR-domain containing prote...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2018.05.004

    authors： Tsoumtsa LL,Sougoufara S,Torre C,Lemichez E,Pontarotti P,Ghigo E

    更新日期：2018-09-01 00:00:00

  • Combinational recognition of bacterial lipoproteins and peptidoglycan by chicken Toll-like receptor 2 subfamily.

    abstract：:Human Toll-like receptor 2 (TLR2) subfamily recognizes bacterial lipoproteins (BLP) and peptidoglycan (PGN). According to the genome information, chicken has structural orthologs of TLRs1 and 2, in addition to TLRs3, 4, 5 and 7. Chicken has two additional TLRs, TLR15 and TLR21, whose orthologs human lacks. The chicken...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2007.05.003

    authors： Higuchi M,Matsuo A,Shingai M,Shida K,Ishii A,Funami K,Suzuki Y,Oshiumi H,Matsumoto M,Seya T

    更新日期：2008-01-01 00:00:00

  • Black carp RIPK1 negatively regulates MAVS-mediated antiviral signaling during the innate immune activation.

    abstract：:Receptor-interacting serine/threonine protein kinase 1 (RIPK1) is an important regulator of necroptosis and involved in innate immune response in human and mammal; however, its function in teleost fish mains largely unknown. In this paper, the RIPK1 homologue of black carp (Mylopharyngodon piceus) has been cloned and ...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2020.103726

    authors： Xie X,Cao Y,Dai Y,Chen Z,Wei J,Tan Y,Wu H,Feng H

    更新日期：2020-08-01 00:00:00

  • A bifunctional invertebrate-type lysozyme from the disk abalone, Haliotis discus discus: genome organization, transcriptional profiling and biological activities of recombinant protein.

    abstract：:Lysozyme is an important enzyme in the innate immune system that plays a vital role in fighting microbial infections. In the current study, we identified, cloned, and characterized a gene that encodes an invertebrate-type lysozyme from the disk abalone, Haliotis discus discus (abLysI). The full-length cDNA of abLysI c...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2013.06.009

    authors： Bathige SD,Umasuthan N,Kasthuri SR,Whang I,Lim BS,Nam BH,Lee J

    更新日期：2013-10-01 00:00:00

  • Immulectin-2, a pattern recognition receptor that stimulates hemocyte encapsulation and melanization in the tobacco hornworm, Manduca sexta.

    abstract：:In insects, encapsulation followed by melanization is a major defense mechanism against metazoan parasites. However, insects must recognize and differentiate nonself before they mount an immune response. Recognition of pathogens in insects is accomplished by a set of pattern recognition receptors (PRRs). Binding of PR...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2004.02.005

    authors： Yu XQ,Kanost MR

    更新日期：2004-07-01 00:00:00

  • Interferon γ and interleukin 10 responses in immunocompetent and immunosuppressed New Zealand White rabbits naturally infected with Encephalitozoon cuniculi.

    abstract：:Levels of interferon (IFN)-γ and interleukin (IL)-10 were measured in the serum of immunocompetent and immunosuppressed New Zealand White rabbits naturally infected with Encephalitozoon cuniculi. IFN-γ levels were elevated in infected rabbits, and a synergic effect was observed in animals treated with the immunosuppre...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2016.05.003

    authors： Rodríguez-Tovar LE,Castillo-Velázquez U,Arce-Mendoza AY,Nevárez-Garza AM,Zarate-Ramos JJ,Hernández-Vidal G,Rodríguez-Ramírez HG,Trejo-Chávez A

    更新日期：2016-09-01 00:00:00

  • The alternatively spliced porcine FcγRI regulated PRRSV-ADE infection and proinflammatory cytokine production.

    abstract：:Receptors for the Fc region of IgG (FcγRs) play a key role in protecting the immune system and host from infection. In this study, we described the cloning, sequencing and characterization of porcine FcγRI, and reported six different FcγRI isoforms, four of which have never been reported before. Further analysis revea...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2018.09.019

    authors： Shi P,Su Y,Li Y,Zhang L,Lu D,Li R,Zhang L,Huang J

    更新日期：2019-01-01 00:00:00

  • Characterization of two novel ADP ribosylation factors from giant freshwater prawn Macrobrachium rosenbergii and their responses to WSSV challenge.

    abstract：:ADP-ribosylation factors (Arfs) are small GTP-binding proteins that have an essential function in intracellular trafficking and organelle structure. To date, little information is available on the Arfs in the economically important giant freshwater prawn Macrobrachium rosenbergii and their relationship to viral infect...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2014.10.006

    authors： Ding ZF,Ren J,Tan JM,Wang Z,Yin SW,Huang Y,Huang X,Wang W,Lan JF,Ren Q

    更新日期：2015-01-01 00:00:00

  • Surface immunoglobulin on granular and agranular leukocytes in the thymus and spleen of the snapping turtle, Chelydra serpentina.

    abstract：:Spleen and thymus suspensions from the turtle, Chelydra serpentina were examined by indirect immunofluorescence and found to contain both agranulocytes and granulocytes positive for surface Ig. Among the splenic agranulocytes , 50% of the lymphocyte and monocyte population was positive for surface Ig. In the thymus on...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/0145-305x(84)90015-6

    authors： Mead KF,Borysenko M

    更新日期：1984-01-01 00:00:00

  • Shark class II invariant chain reveals ancient conserved relationships with cathepsins and MHC class II.

    abstract：:The invariant chain (Ii) is the critical third chain required for the MHC class II heterodimer to be properly guided through the cell, loaded with peptide, and expressed on the surface of antigen presenting cells. Here, we report the isolation of the nurse shark Ii gene, and the comparative analysis of Ii splice varia...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2011.09.008

    authors： Criscitiello MF,Ohta Y,Graham MD,Eubanks JO,Chen PL,Flajnik MF

    更新日期：2012-03-01 00:00:00

  • Postnatal changes in epigenetic modifications of neutrophils of foals are associated with increased ROS function and regulation of neutrophil function.

    abstract：:Neonates of all species, including foals, are highly susceptible to infection, and neutrophils play a crucial role in innate immunity to infection. Evidence exists that neutrophils of neonatal foals are functionally deficient during the first weeks of life, including expression of cytokine genes such as IFNG. We hypot...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2018.06.012

    authors： Dindot SV,Doan RN,Kuskie KR,Hillman PR,Whitfield CM,McQueen CM,Bordin AI,Bourquin JR,Cohen ND

    更新日期：2018-10-01 00:00:00

  • To complete its replication cycle, a shrimp virus changes the population of long chain fatty acids during infection via the PI3K-Akt-mTOR-HIF1α pathway.

    abstract：:White spot syndrome virus (WSSV), the causative agent of white spot disease (WSD), is a serious and aggressive shrimp viral pathogen with a worldwide distribution. At the genome replication stage (12 hpi), WSSV induces a metabolic rerouting known as the invertebrate Warburg effect, which boosts the availability of ene...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2015.06.001

    authors： Hsieh YC,Chen YM,Li CY,Chang YH,Liang SY,Lin SY,Lin CY,Chang SH,Wang YJ,Khoo KH,Aoki T,Wang HC

    更新日期：2015-11-01 00:00:00

  • Antimicrobial peptide defenses against chytridiomycosis, an emerging infectious disease of amphibian populations.

    abstract：:Chytridiomycosis, an emerging infectious disease (EID) of the skin caused by the chytrid fungus, Batrachochytrium dendrobatidis, has been linked with continuing amphibian population declines in the western USA, Central America, Europe, Africa, and Australia. Genetic analysis suggests that B. dendrobatidis is a recentl...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章,评审

    doi：10.1016/j.dci.2004.11.004

    authors： Rollins-Smith LA,Conlon JM

    更新日期：2005-01-01 00:00:00

  • Phenoloxidase and QX disease resistance in Sydney rock oysters (Saccostrea glomerata).

    abstract：:QX is a fatal disease in Sydney rock oysters (Saccostrea glomerata) that results from infection by the protistan parasite, Marteilia sydneyi. Since 1997, the New South Wales Fisheries Service has bred S. glomerata for resistance to QX disease. The current study shows that the QX resistance breeding program has selecte...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2003.10.004

    authors： Newton K,Peters R,Raftos D

    更新日期：2004-05-17 00:00:00

  • Receptors for complement on echinoid phagocytes. I. The opsonic effect of vertebrae sera on echinoid phagocytosis.

    abstract：:The ingestion of sheep erythrocytes (SRBC) by echinoid phagocytes was greatly increased after treatment of SRBC with sera from mouse, human and fish. The opsonic principle in the sera was heat labile (56 degrees C 1/2 hr). Opsonization with mammalian sera was studied more extensively. It was dependent on pre-sensitiza...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/s0145-305x(82)80028-1

    authors： Bertheussen K,Seljelid R

    更新日期：1982-07-01 00:00:00

  • Partial characterization of different cell types found in the Xenopus laevis lymphoreticular tumor based on the presence or absence of surface immunoglobulins and Fc molecules.

    abstract：:Cells of Xenopus laevis lymphoreticular tumor induced by tumor tissue transplantation were examined for surface Ig and Fc receptor molecules in order to evaluate the different cell types found in the tumor. Direct immunofluorescent technique, using fluorochrome conjugated rabbit antisera to Xenopus Ig's, detected Ig m...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/0145-305x(86)90176-x

    authors： Coosemans V,Hadji-Azimi I

    更新日期：1986-10-01 00:00:00

  • Hydrocortisone suppresses the chemiluminescent response of striped bass phagocytes.

    abstract：:Phagocytic cells obtained from the pronephros of striped bass (Morone saxatilis) were exposed in vitro to various levels of hydrocortisone acetate. This treatment reduced the normal ability of the cells to generate a chemiluminescent response when exposed to bacteria or phorbol myristate acetate. Although the levels o...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/0145-305x(85)90061-8

    authors： Stave JW,Roberson BS

    更新日期：1985-01-01 00:00:00

  • Functional characterization of STATa/b genes encoding transcription factors from Branchiostoma belcheri.

    abstract：:The signal transducer and activator of transcription (STAT), as an important transcription factor of the Janus kinase (JAK)-STAT signaling pathway, is pivotal for development and immunity and well documented in vertebrates. However, the STAT gene has not been reported in chordate amphioxus (Branchiostoma belcheri). In...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2020.103838

    authors： Cao Y,Fang T,Fan M,Wang L,Lv C,Xiaojun Song,Jin P,Ma F

    更新日期：2021-01-01 00:00:00

  • Identification and functional characterization of the house finch interleukin-1β.

    abstract：:Interleukin-1β (IL-1β), an inflammatory cytokine of the IL-1 family, is primarily produced as a precursor protein by monocytes and macrophages, then matures and becomes activated through proteolytic catalysis. Although the biological characteristics of avian IL-1β are well known, little information is available about ...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/j.dci.2016.12.004

    authors： Park M,Kim S,Adelman JS,Leon AE,Hawley DM,Dalloul RA

    更新日期：2017-04-01 00:00:00

  • Humoral immunoglobulins of the white sturgeon, Acipenser transmontanus: partial characterization of and recognition with monoclonal antibodies.

    abstract：:White sturgeon (Acipenser transmontanus) immunoglobulin (Ig) was purified from serum by two methods, ion-exchange chromatography and gel filtration and precipitation of the euglobulin fraction. The purity of these immunoglobulin preparations was confirmed by gel electrophoresis. Sequence analysis of the N-terminal ami...

    journal_title：Developmental and comparative immunology

    pub_type： 杂志文章

    doi：10.1016/0145-305x(96)00015-8

    authors： Adkison MA,Basurco B,Hedrick RP

    更新日期：1996-07-01 00:00:00