The alternatively spliced porcine FcγRI regulated PRRSV-ADE infection and proinflammatory cytokine production.


:Receptors for the Fc region of IgG (FcγRs) play a key role in protecting the immune system and host from infection. In this study, we described the cloning, sequencing and characterization of porcine FcγRI, and reported six different FcγRI isoforms, four of which have never been reported before. Further analysis revealed that FcγR isoforms are generated by alternative splicing mechanisms, including two membrane isoforms and four soluble isoforms. Importantly, we found FcγRI splice variants differentially influence PRRSV antibody-dependent enhancement (ADE) effects. Membrane pCD64-T1 promotes endocytosis of the PRRSV-antibody complex to enhance PRRSV replication, and soluble pCD64-T3 has no ADE effect on PRRSV proliferation, but shows an inflammation enhancement effect. The differential expression of selective splicing in primary PAM cells and 3D4/21 cell lines are altered and regulated by PRRSV infection and inflammatory environment. Our results indicated that porcine FcγRI plays dual regulatory roles in PRRSV multiplication and PRRSV inflammation process by the alternatively spliced mechanism, which will be a new target in PRRSV prevention and control.


Dev Comp Immunol


Shi P,Su Y,Li Y,Zhang L,Lu D,Li R,Zhang L,Huang J




Has Abstract


2019-01-01 00:00:00












  • Development of B cells in the gut-associated lymphoid tissue of mid-gestational fetal lambs.

    abstract::Intestinal mucosal immune system development was investigated in fetal lambs from 61 to 110 days (term, 150 days). Fetal small intestine was examined at 400 mm intervals for the presence of IgM(+) cells. The first phase of B cell development was characterised by increase in B cell density throughout the jejunum from 6...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Alitheen N,McClure S,McCullagh P

    更新日期:2003-06-01 00:00:00

  • The M2 macrophages induce autophagic vascular disorder and promote mouse sensitivity to urethane-related lung carcinogenesis.

    abstract::Tumor vessels are known to be abnormal, with typically aberrant, leaky and disordered vessels. Here, we investigated whether polarized macrophage phenotypes are involved in tumor abnormal angiogenesis and what is its mechanism. We found that there was no difference in chemotaxis of polarized M1 and M2 macrophages to l...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Li GG,Guo ZZ,Ma XF,Cao N,Geng SN,Zheng YQ,Meng MJ,Lin HH,Han G,Du GJ

    更新日期:2016-06-01 00:00:00

  • Conserved inhibitory role of teleost SOCS-1s in IFN signaling pathways.

    abstract::The suppressor of cytokine signaling 1 (SOCS-1) protein is a critical regulator in the immune systems of humans and mammals, which functions classically as an inhibitor of the IFN signaling pathways. However, data on functional characterisation of SOCS-1 in ancient vertebrates are limited. In this study, we report the...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Nie L,Xiong R,Zhang YS,Zhu LY,Shao JZ,Xiang LX

    更新日期:2014-03-01 00:00:00

  • Alternate forms of MHC class II-associated invariant chain are not produced by alternative splicing in rainbow trout (Oncorhynchus mykiss) but are encoded by separate genes.

    abstract::A major limiting factor in understanding teleost major histocompatibility receptor function is the lack of knowledge about antigen presentation accessory molecules. We report here two cDNA clones encoding teleost versions of invariant chain and one encoding a related protein that may play a protease inhibition role in...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Fujiki K,Smith CM,Liu L,Sundick RS,Dixon B

    更新日期:2003-05-01 00:00:00

  • Partial characterization of different cell types found in the Xenopus laevis lymphoreticular tumor based on the presence or absence of surface immunoglobulins and Fc molecules.

    abstract::Cells of Xenopus laevis lymphoreticular tumor induced by tumor tissue transplantation were examined for surface Ig and Fc receptor molecules in order to evaluate the different cell types found in the tumor. Direct immunofluorescent technique, using fluorochrome conjugated rabbit antisera to Xenopus Ig's, detected Ig m...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Coosemans V,Hadji-Azimi I

    更新日期:1986-10-01 00:00:00

  • A novel C-type lectin (FcLec4) facilitates the clearance of Vibrio anguillarum in vivo in Chinese white shrimp.

    abstract::C-type lectins play important roles in innate immunity of invertebrates. In the present study, we report a novel C-type lectin, named FcLec4, from the Chinese white shrimp Fenneropenaeus chinensis. FcLec4 contains a single carbohydrate recognition domain (CRD) with a putative signal peptide. Phylogenetic analysis indi...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Wang XW,Zhang XW,Xu WT,Zhao XF,Wang JX

    更新日期:2009-09-01 00:00:00

  • Cloning of Litopenaeus vannamei CD63 and it's role in white spot syndrome virus infection.

    abstract::White Spot Syndrome Virus (WSSV) is currently the most serious shrimp pathogen, which has brought huge losses to shrimp industry worldwide. CD63 of shrimp belongs to the tetraspanin superfamily, which plays an important role in signal transduction and immune process. In this paper, CD63 cDNA sequence of Litopenaeus va...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Guan GK,Liu QH,Li C,Huang J

    更新日期:2016-07-01 00:00:00

  • A Kazal-type serine proteinase inhibitor SPIPm2 from the black tiger shrimp Penaeus monodon is involved in antiviral responses.

    abstract::A five-domain Kazal-type serine proteinase inhibitor, SPIPm2, from Penaeus monodon has recently been implicated in antiviral responses for it is up-regulated upon viral infection and needs further studies. The SPIPm2 genomic gene was composed of seven exons and six introns. The genomic DNA segments coding for each Kaz...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Donpudsa S,Ponprateep S,Prapavorarat A,Visetnan S,Tassanakajon A,Rimphanitchayakit V

    更新日期:2010-10-01 00:00:00

  • Functional characterization of avidins in amphioxus Branchiostoma japonicum: Evidence for a dual role in biotin-binding and immune response.

    abstract::Avidin is well known for its high affinity to biotin and has been found in many egg-laying vertebrate species. However, little is known about avidin in invertebrate species to date. Here we clearly showed the presence of two avidin genes, Bjavidin1 and Bjavidin2, in the amphioxus Branchiostoma japonicum, the first one...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Guo X,Xin J,Wang P,Du X,Ji G,Gao Z,Zhang S

    更新日期:2017-05-01 00:00:00

  • Molecular and functional characterization of erythropoietin receptor of the goldfish (Carassius auratus L.).

    abstract::Erythropoietin receptor (EPOR) is a member of the class I cytokine receptor superfamily and signaling through this receptor is important for the proliferation, differentiation and survival of erythrocyte progenitor cells. This study reports on the molecular and functional characterization of goldfish EPOR. The identif...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Katakura F,Katzenback BA,Belosevic M

    更新日期:2014-08-01 00:00:00

  • Ontogeny of T-cell mitogen response in Lewis rats: II. Early appearance and loss of suppressor activity.

    abstract::Spleen cells from rats 2 to 132 days old were cultured with 1-125 micrograms/ml Concanavalin A (Con A). At high doses of Con A, the high spontaneous thymidine uptake of spleen cells from rats 15 to 21 days old was suppressed, whereas spleen cells from younger rats showed no suppression of spontaneous mitogenesis at eq...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Middleton PA,Bullock WW

    更新日期:1984-10-01 00:00:00

  • Ultrastructural localization of a soluble antigen in the chicken Harderian gland.

    abstract::The relationship between plasma cells, macrophages, B and T cells, dendritic cells, and epithelium in the chicken Harderian gland have been studied by means of ultrastructural localization of the horseradish peroxidase following local immunization. After 5 d, peroxidase activity was found in vesicles located in macrop...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: del Cacho E,Gallego M,Bascuas JA

    更新日期:1992-03-01 00:00:00

  • Evolution of classic Hodgkin lymphoma in correlation to changes in the lymphoid organ structure of vertebrates.

    abstract::In order to integrate evolutionary concepts into lymphoma research we mapped features of classic Hodgkin lymphoma (a disease which has been recently described to be derived from germinal center B-cells) onto a phylogenetic tree of vertebrates. Secondly, we matched the phylogenetic occurrence of classic Hodgkin lymphom...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章,评审


    authors: Seitz V,Hummel M,Walter J,Stein H

    更新日期:2003-01-01 00:00:00

  • Molecular cloning and characterization of novel cathelicidin-derived myeloid antimicrobial peptide from Phasianus colchicus.

    abstract::Cathelicidins were initially characterized as a family of antimicrobial peptides. Now it is clear that they fulfill several immune functions in addition to their antimicrobial activity. In the current work, three cDNA sequences encoding pheasant cathelicidins were cloned from a constructed bone marrow cDNA library of ...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Wang Y,Lu Z,Feng F,Zhu W,Guang H,Liu J,He W,Chi L,Li Z,Yu H

    更新日期:2011-03-01 00:00:00

  • Establishment and characterization of two head kidney macrophage cell lines from large yellow croaker (Larimichthys crocea).

    abstract::Two continuous macrophage cell lines (LCM07 and LCM10) were established for the first time from the head kidney of the marine fish large yellow croaker (Larimichthys crocea). To date, both cell lines have been subcultured for more than 100 passages in 12 months. Notably, the LCM07 and LCM10 cells have distinct morphol...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Cui K,Li Q,Xu D,Zhang J,Gao S,Xu W,Mai K,Ai Q

    更新日期:2020-01-01 00:00:00

  • Relationship between circulating thyroid hormones and humoral immunity in immature male chickens.

    abstract::The objective of this experiment was to examine the relationship between levels of circulating T3, T4, and humoral immunity in immature male chickens. Three week old Single Comb White Leghorn male chicks were used as the experimental animals. In order to produce a wide range of circulating thyroid hormone concentratio...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Bachman SE,Mashaly MM

    更新日期:1986-07-01 00:00:00

  • Loss of CD28 expression on T lymphocytes: a marker of replicative senescence.

    abstract::The CD28 molecule, a disulfide-linked homodimer expressed on peripheral T cells and thymocytes, mediates an essential costimulatory signal following engagement of the T cell receptor (TCR). Increased proportions of CD28- T cells have been observed during aging and in situations of chronic immune stimulation, but the o...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Effros RB

    更新日期:1997-11-01 00:00:00

  • miRNAs associated with immune response in teleost fish.

    abstract::MicroRNAs (miRNAs) have been identified as important post transcriptional regulators of gene expression. In higher vertebrates, a subset of miRNAs has been identified as important regulators of a number of key genes in immune system gene networks, and this paper review recent studies on miRNAs associated with immune r...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章,评审


    authors: Andreassen R,Høyheim B

    更新日期:2017-10-01 00:00:00

  • Pre-acute hepadnaviral infection is associated with activation-induced apoptotic death of lymphocytes in the woodchuck (Marmota monax) model of hepatitis B.

    abstract::Woodchucks (Marmota monax) infected with woodchuck hepatitis virus (WHV) represent a highly valuable immunopathogenic model of hepatitis B virus (HBV) infection. Both WHV and HBV are noncytopathic hepadnaviruses which induce a strong but delayed virus-specific cellular immune response believed to be a cause of hepatit...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Gujar SA,Jenkins AK,Macparland SA,Michalak TI

    更新日期:2010-09-01 00:00:00

  • Activation of immune-associated phospholipase A2 is functionally linked to Toll/Imd signal pathways in the red flour beetle, Tribolium castaneum.

    abstract::Bacterial challenge enhances phospholipase A(2) (PLA(2)) activity, which in turn induces biosynthesis of various eicosanoids that mediate non-self recognition signal to immune effectors in insects. But, there is little information on how PLA(2) activity is controlled after the non-self recognition. A recent genome ana...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Shrestha S,Kim Y

    更新日期:2010-05-01 00:00:00

  • JAK and STAT members in channel catfish: Identification, phylogenetic analysis and expression profiling after Edwardsiella ictaluri infection.

    abstract::The Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling pathway is one of the main pleiotropic cascades used to transmit information from extracellular receptors to the nucleus, which results in DNA transcription and expression of genes involved in immunity, proliferation, differentiat...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Jin Y,Zhou T,Li N,Liu S,Xu X,Pan Y,Tan S,Shi H,Yang Y,Yuan Z,Wang W,Luo J,Gao D,Dunham R,Liu Z

    更新日期:2018-04-01 00:00:00

  • Comparison of suppressor cells obtained from tumor-allosensitized adult and untreated neonatal mice.

    abstract::Splenic suppressor cells from neonatal ("intrinsic") and adult (tumor-allosensitized) mice were compared. Both are non-antigen specific and non-H-2 restricted, and are Ly1+2+, I-J+, QA2+ T-cells. Injection of adult peritoneal exudate cells into neonates or adult tumor-allosensitized mice decreases suppressor cell acti...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Argyris BF

    更新日期:1985-01-01 00:00:00

  • Identification of a novel antimicrobial peptide from the sea star Patiria pectinifera.

    abstract::Antimicrobial peptides (AMPs) are components of innate immunity found in many forms of life. However, there have been no reports of AMPs in sea star (Phylum Echinodermata). Here we report the isolation and characterization of a novel antimicrobial peptide from the coelomic epithelium extract of the sea star Patiria pe...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Kim CH,Go HJ,Oh HY,Park JB,Lee TK,Seo JK,Elphick MR,Park NG

    更新日期:2018-09-01 00:00:00

  • DNA content and proliferation of rat thymocytes.

    abstract::Scanning microdensitometric analysis of Feulgen stained thymocytes from rats aged 1 to 71 days revealed the presence of a number of waves of cell proliferation which may correlate with the development of functionally distinct T-cell subpopulations. In addition, considerable numbers of hypodiploid cells were seen. It i...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Rippin JW

    更新日期:1984-01-01 00:00:00

  • Two plasmolipins from the black tiger shrimp, Penaeus monodon and their response to virus pathogens.

    abstract::Two isoforms of plasmolipin were initially identified from the black tiger shrimp (Penaeus monodon) EST database and completed using 50 RACE to reveal complete cDNAs of 558 bp (PmPLP1) and 537 bp(PmPLP2) with 87% nucleotide sequence identity. The deduced amino acid sequences contained four-transmembrane domains and sh...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Vatanavicharn T,Pongsomboon S,Tassanakajon A

    更新日期:2012-10-01 00:00:00

  • Expression of an inducible nitric oxide synthase gene in rainbow trout Oncorhynchus mykiss.

    abstract::Using oligonucleotide primers based on mammalian nitric oxide synthases (NOS), expression of an inducible NOS (iNOS) gene was detected in head kidney and gill tissue of bacterially-challenged rainbow trout. Three overlapping fragments were amplified by RT-PCR and used to construct a contiguous sequence of 1410bp, with...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Laing KJ,Hardie LJ,Aartsen W,Grabowski PS,Secombes CJ

    更新日期:1999-01-01 00:00:00

  • The inhibitory effect of environmental ammonia on Danio rerio LPS induced acute phase response.

    abstract::Ammonia is a toxic by-product of amino acid catabolism and a common environmental pollutant that has been associated with increased disease susceptibility in fish although the mechanism is not well understood. We addressed the hypothesis that elevated environmental ammonia acts by impairing the acute phase response (A...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Gonçalves AF,Páscoa I,Neves JV,Coimbra J,Vijayan MM,Rodrigues P,Wilson JM

    更新日期:2012-02-01 00:00:00

  • The compatibility of Xenopus antibody and homoiothermic complement.

    abstract::Adult Xenopus laevis serum containing an anti-chicken erythrocyte antibody exhibits complement (C) activity by both the classical and the alternative pathways. Lytic activity is removed by heating serum to 50 degrees C for at least 20 min; it is also removed by polysaccharides and by 7.5 mM EDTA. The optimum temperatu...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: McKnight BJ,Jurd RD

    更新日期:1987-07-01 00:00:00

  • Hydrocortisone suppresses the chemiluminescent response of striped bass phagocytes.

    abstract::Phagocytic cells obtained from the pronephros of striped bass (Morone saxatilis) were exposed in vitro to various levels of hydrocortisone acetate. This treatment reduced the normal ability of the cells to generate a chemiluminescent response when exposed to bacteria or phorbol myristate acetate. Although the levels o...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Stave JW,Roberson BS

    更新日期:1985-01-01 00:00:00

  • Identification and characterization of an amphioxus matrix metalloproteinase homolog BbMMPL2 responding to bacteria challenge.

    abstract::Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases mainly involved in extracellular matrix (ECM) degradation. We have cloned and identified BbMMPL2 as homolog of MMPs from adult amphioxus. Recombinant BbMMPL2 proteins underwent self-processing during refolding in vitro. The final ~23 kDa po...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Zhang Y,Zhang H,Kong Y,Feng L

    更新日期:2012-07-01 00:00:00