The alternatively spliced porcine FcγRI regulated PRRSV-ADE infection and proinflammatory cytokine production.


:Receptors for the Fc region of IgG (FcγRs) play a key role in protecting the immune system and host from infection. In this study, we described the cloning, sequencing and characterization of porcine FcγRI, and reported six different FcγRI isoforms, four of which have never been reported before. Further analysis revealed that FcγR isoforms are generated by alternative splicing mechanisms, including two membrane isoforms and four soluble isoforms. Importantly, we found FcγRI splice variants differentially influence PRRSV antibody-dependent enhancement (ADE) effects. Membrane pCD64-T1 promotes endocytosis of the PRRSV-antibody complex to enhance PRRSV replication, and soluble pCD64-T3 has no ADE effect on PRRSV proliferation, but shows an inflammation enhancement effect. The differential expression of selective splicing in primary PAM cells and 3D4/21 cell lines are altered and regulated by PRRSV infection and inflammatory environment. Our results indicated that porcine FcγRI plays dual regulatory roles in PRRSV multiplication and PRRSV inflammation process by the alternatively spliced mechanism, which will be a new target in PRRSV prevention and control.


Dev Comp Immunol


Shi P,Su Y,Li Y,Zhang L,Lu D,Li R,Zhang L,Huang J




Has Abstract


2019-01-01 00:00:00












  • Demonstration of T cell and macrophage progenitors in carp (Cyprinus carpio) kidney hematopoietic tissues. Development of clonal assay system for carp hematopoietic cells.

    abstract::Single hematopoietic cells from carp (Cyprinus carpio) kidney were seeded to each well of 96-well plates and cultured in the presence of a supporting cell layer and conditioned media (CM). The CM were obtained from bulk-cultured carp hematopoietic cells, in which T and macrophage-lineage cells rapidly proliferated as ...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Katakura F,Yamaguchi T,Yoshida M,Moritomo T,Nakanishi T

    更新日期:2010-06-01 00:00:00

  • The immune system of sea bass, Dicentrarchus labrax, reared in aquaculture.

    abstract::The sea bass Dicentrarchus labrax is one the most important seawater fish species of south Europe and Mediterranean aquaculture, and studies on its immune system are important for both scientific and applied purposes. In this paper, we summarise the results obtained in studies of the immune system in this species, and...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章,评审


    authors: Scapigliati G,Romano N,Buonocore F,Picchietti S,Baldassini MR,Prugnoli D,Galice A,Meloni S,Secombes CJ,Mazzini M,Abelli L

    更新日期:2002-03-01 00:00:00

  • WITHDRAWN: Development of an RT-PCR-RFLP assay for the detection and differentiation of wild-type and vaccine strains of classical swine fever virus.

    abstract::This article has been withdrawn at the request of the author(s). The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at ...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Hu J,Shan H,Wang S,Yang P,Wang Y,Sun H,Huang J,Yang R,Zhang C,Qin X

    更新日期:2012-05-15 00:00:00

  • Bax functions as coelomocyte apoptosis regulator in the sea cucumber Apostichopus japonicus.

    abstract::Bcl-2-associated X (Bax) belongs to the Bcl-2 protein family and its pro-apoptotic function has been confirmed in many vertebrate species. However, the functional role of Bax in apoptosis in invertebrates is limited. Here, a Bax homologue (AjBax) in Apostichopus japonicas was cloned and characterized, and its pro-apop...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Guo M,Lv M,Shao Y,Zhang W,Zhao X,Li C

    更新日期:2020-01-01 00:00:00

  • Comparative ontogenetic development of two marine teleosts, gilthead seabream and European sea bass: New insights into nutrition and immunity.

    abstract::Gilthead seabream and European sea bass are two of the most commonly farmed fish species. Larval development is critical to ensure high survival rates and thus avoid unacceptable economic losses, while nutrition and immunity are also important factors. For this reason this paper evaluates the ontogenetic development o...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Cordero H,Guzmán-Villanueva LT,Chaves-Pozo E,Arizcun M,Ascencio-Valle F,Cuesta A,Esteban MA

    更新日期:2016-12-01 00:00:00

  • Molecular characterization and expression analysis of four isotypes of immunoglobulin light chain genes in orange-spotted grouper, Epinephelus coioides.

    abstract::To date, many immunoglobulin (Ig) genes have been identified in diverse teleost species, but the contributions of different types of light chain (IgL) to the immune response remain unclear. Screening of a stimulated kidney cDNA library from orange-spotted grouper (Osg, Epinephelus coioides) resulted in the identificat...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Wu MS,Cheng CA,Lin CH,Lee CY,Tseng SJ,Tzeng CS,Chang CY

    更新日期:2013-03-01 00:00:00

  • Immunity against selected piscine flagellates.

    abstract::This discussion is on immune response to Amyloodinium ocellatum, Cryptobia salmositica, Trypanoplasma borreli and Trypanosoma carassii. Piscidin and histone-like proteins enhance innate resistance to Amyloodinium. Fish that are naturally resistant to Cryptobia and Trypanoplasma can be bred. Cryptobia resistance in cha...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章,评审


    authors: Woo PT,Ardelli BF

    更新日期:2014-04-01 00:00:00

  • The upregulation of immune responses in tyrosine hydroxylase (TH) silenced Litopenaeus vannamei.

    abstract::Catecholamines (CAs) play a crucial role in maintaining physiological and immune homeostasis in invertebrates and vertebrates under stressful conditions. Tyrosine hydroxylase (TH) is the first and rate-limiting enzyme in CA synthesis. To develop an effective CA-related immunological defense system against stress and p...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Mapanao R,Chang CC,Cheng W

    更新日期:2017-02-01 00:00:00

  • CsTNF1, a teleost tumor necrosis factor that promotes antibacterial and antiviral immune defense in a manner that depends on the conserved receptor binding site.

    abstract::Tumor necrosis factor (TNF) is one of the most important cytokines involved in inflammation, apoptosis, cell proliferation, and stimulation of the immune system. The TNF gene has been cloned in teleost fish; however, the in vivo function of fish TNF is essentially unknown. In this study, we report the identification o...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Li MF,Zhang J

    更新日期:2016-02-01 00:00:00

  • The PHD domain of the sea urchin RAG2 homolog, SpRAG2L, recognizes dimethylated lysine 4 in histone H3 tails.

    abstract::V(D)J recombination is a somatic gene rearrangement process that assembles antigen receptor genes from individual segments during lymphocyte development. The access of the RAG1/RAG2 recombinase to these gene segments is regulated at the level of chromatin modifications, in particular histone tail modifications. Trimet...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Wilson DR,Norton DD,Fugmann SD

    更新日期:2008-01-01 00:00:00

  • Identification and characterization of a novel phage-type like lysozyme from Manila clam, Ruditapes philippinarum.

    abstract::A novel lysozyme gene (RpLysPh) with high similarity to the bacteriophage lysozymes was identified in Manila clam, Ruditapes philippinarum. The full length cDNA of RpLysPh is 828bp and contains a 462bp open reading frame (ORF) that codes for a 154 amino acid protein. Multiple sequence alignment analysis revealed that ...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Ding J,Wang R,Yang F,Zhao L,Qin Y,Zhang G,Yan X

    更新日期:2014-11-01 00:00:00

  • Molecular cloning and characterisation of the griffon vulture (Gyps fulvus) toll-like receptor 1.

    abstract::The toll-like receptor (TLR) family is an ancient pattern recognition receptor family, conserved from insects to mammals. Members of the TLR family are vital to immune function through the sensing of pathogenic agents and initiation of an appropriate immune response. In this study, we cloned a cDNA encoding for a grif...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: de la Lastra JM,de la Fuente J

    更新日期:2007-01-01 00:00:00

  • Lectins and lectin-like molecules in lower plants. II. Freshwater algae.

    abstract::Freshwater chlamydomonad algae possess flagella surface isoagglutinins of a glycoprotein nature which are involved in gamete recognition and consequent cell fusion. Alternatively, such molecules may have a receptor role in certain Chlamydomonas species. In some freshwater volvocaines, lectin-like substances act as ind...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章,评审


    authors: Ingram GA

    更新日期:1985-07-01 00:00:00

  • Evolution of classic Hodgkin lymphoma in correlation to changes in the lymphoid organ structure of vertebrates.

    abstract::In order to integrate evolutionary concepts into lymphoma research we mapped features of classic Hodgkin lymphoma (a disease which has been recently described to be derived from germinal center B-cells) onto a phylogenetic tree of vertebrates. Secondly, we matched the phylogenetic occurrence of classic Hodgkin lymphom...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章,评审


    authors: Seitz V,Hummel M,Walter J,Stein H

    更新日期:2003-01-01 00:00:00

  • The ontogeny of the porcine immune system.

    abstract::Cellular and humoral aspects of the immune response develop sequentially in the fetus. During the ontogeny, the pluripotent stem cells emerge and differentiate into all hematopoietic lineages. Basic questions including the identification of the first lympho-hematopoietic sites, the origin of T and B lymphocytes, the d...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章,评审


    authors: Sinkora M,Butler JE

    更新日期:2009-03-01 00:00:00

  • Development of the immune system and immunological protection in marsupial pouch young.

    abstract::At birth the tissues of marsupial immune system are underdeveloped. The young animal is not immunocompetent. Histological and immunohistochemical studies of pouch young epithelial tissues provide a clear picture of tissue development but the timing of onset of immunocompetence awaits definition. The survival of the ne...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章,评审


    authors: Old JM,Deane EM

    更新日期:2000-07-01 00:00:00

  • Carbohydrate binding specificity and purification by biospecific affinity chromatography of Ascidia malaca Traust. hemagglutinins.

    abstract::The carbohydrate specificities of Ascidia malaca serum hemagglutinins were determined by hemagglutination inhibition tests. Analysis of agglutinins against rabbit and human A, B, O erythrocytes suggests that the size of the combining site corresponds to a disaccharide with a specificity for saccharides containing a D...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Parrinello N,Canicattì C

    更新日期:1982-01-01 00:00:00

  • Investigations on the role of a lysozyme from the malaria vector Anopheles dirus during malaria parasite development.

    abstract::A cDNA encoding a lysozyme was obtained by rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR) from females of the malaria vector Anopheles dirus A (Diptera: Culicidae). The 623 bp lysozyme (AdLys c-1) cDNA encodes the 120 amino acid mature protein with a predicted molecular mass of 13.4 kDa and theo...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Lapcharoen P,Komalamisra N,Rongsriyam Y,Wangsuphachart V,Dekumyoy P,Prachumsri J,Kajla MK,Paskewitz SM

    更新日期:2012-01-01 00:00:00

  • Effect of experimental microbial challenge on the expression of defense molecules in Eisenia foetida earthworm.

    abstract::Earthworms are able to protect themselves against invading pathogens due to efficient innate defense mechanisms. Currently, two types of antimicrobial factors including lysozyme-like molecule and factors with hemolytic activity, as well as a pattern recognition protein named coelomic cytolytic factor (CCF) have been i...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Köhlerová P,Beschin A,Silerová M,De Baetselier P,Bilej M

    更新日期:2004-06-01 00:00:00

  • Ecotoxicology and innate immunity in fish.

    abstract::This review summarizes the scattered literature on the effects of toxicants on the external and internal innate immunity of fish. Insecticides, heavy metals and surfactants have been the most frequently examined toxicants, whereas dioxins, furans and polychlorinated biphenyls have been tested less frequently. Studies ...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章,评审


    authors: Bols NC,Brubacher JL,Ganassin RC,Lee LE

    更新日期:2001-10-01 00:00:00

  • Exploring the immune signalling pathway-related genes of the cattle tick Rhipicephalus microplus: From molecular characterization to transcriptional profile upon microbial challenge.

    abstract::In dipteran insects, invading pathogens are selectively recognized by four major pathways, namely Toll, IMD, JNK, and JAK/STAT, and trigger the activation of several immune effectors. Although substantial advances have been made in understanding the immunity of model insects such as Drosophila melanogaster, knowledge ...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Rosa RD,Capelli-Peixoto J,Mesquita RD,Kalil SP,Pohl PC,Braz GR,Fogaça AC,Daffre S

    更新日期:2016-06-01 00:00:00

  • Serum amyloid A transcription in Atlantic salmon (Salmo salar L.) hepatocytes is enhanced by stimulation with macrophage factors, recombinant human IL-1 beta, IL-6 and TNF alpha or bacterial lipopolysaccharide.

    abstract::Serum amyloid A (A-SAA) has previously been reported to be an acute-phase protein in salmonids. Hepatocytes represent a major source of A-SAA in salmonids, but nothing is known about hepatocyte SAA synthesis in fish. In the present work, the expression of A-SAA transcripts in primary cultures of Atlantic salmon hepato...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Jørgensen JB,Lunde H,Jensen L,Whitehead AS,Robertsen B

    更新日期:2000-09-01 00:00:00

  • Molecular and functional characterization of a mannose-binding lectin/ficolin-associated protein (MAp44) from Nile tilapia (Oreochromis niloticus) involved in the immune response to bacterial infection.

    abstract::The lectin pathway of the complement system has a pivotal role in the defense against infectious organisms. Mannose-binding lectin/ficolin-associated protein (MAp44), a multifunctional complement regulator, regulates the complement activation by competing with MASP-1, MASP-2 and MASP-3 for MBL and ficolin binding site...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Mu L,Wu H,Han K,Wu L,Bian X,Li B,Guo Z,Yin X,Ye J

    更新日期:2019-12-01 00:00:00

  • Molecular and functional characterisation of a mannose-binding lectin-like gene from Japanese sea bass (Lateolabrax japonicus).

    abstract::Mannose-binding lectin (MBL) plays an important role in host immune responses against pathogens. LjMBL-like-1 was identified from Japanese sea bass (Lateolabrax japonicas), which has selectivity for galactose. Herein, this lectin might be better designated as galactose-binding lectin (LjGalBL-1). LjGalBL-1 transcripts...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Dang Y,Nie L,Chen J

    更新日期:2020-03-01 00:00:00

  • Isolation and initial characterisation of complement components C3 and C4 of the nurse shark and the channel catfish.

    abstract::Complement components C3 and C4 have been isolated from the serum of the nurse shark (Ginglymostoma cirratum) and of the channel catfish (Ictalurus punctatus). As in the higher vertebrates, the fish C4 proteins have three-chain structures while the C3 proteins have two-chain structures. All four proteins have intra-ch...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Dodds AW,Smith SL,Levine RP,Willis AC

    更新日期:1998-03-01 00:00:00

  • B-cell development in bovine fetuses proceeds via a pre-B like cell in bone marrow and lymph nodes.

    abstract::The production of B cells and the primary antibody repertoire in mammalian species other than rodents or man appears to depend on gut-associated lymphoid tissue. Bovine B cells are generated in ileal Peyer's patch from late gestational to juvenile age. However, little is known about where and when the bona fide B lymp...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Ekman A,Pessa-Morikawa T,Liljavirta J,Niku M,Iivanainen A

    更新日期:2010-08-01 00:00:00

  • Purification and binding properties of porcine plasma ficolin that binds Actinobacillus pleuropneumoniae.

    abstract::Previous studies demonstrated that porcine plasma ficolin binds the important pig pathogen Actinobacillus pleuropneumoniae (APP) in an N-acetylglucosamine-dependent manner. In the present study, attempts to characterize the bacterial-binding properties of ficolin indicated ficolin is the major porcine plasma protein t...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Brooks AS,DeLay JP,Hayes MA

    更新日期:2003-10-01 00:00:00

  • Identification and characterization of chicken interleukin-16 cDNA.

    abstract::Interleukin-16 is an inflammatory cytokine synthesized as a precursor protein (pro-IL-16). Based on sequence data from an EST cDNA library prepared from intestinal intraepithelial lymphocytes of Eimeria-infected chickens, we identified a cDNA that contained a full-length open reading frame of pro-IL-16. The encoded pr...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Min W,Lillehoj HS

    更新日期:2004-02-01 00:00:00

  • In silico analysis of Schmidtea mediterranea TIR domain-containing proteins.

    abstract::While genetic evidence points towards an absence of Toll-Like Receptors (TLRs) in Platyhelminthes, the Toll/IL-1 Receptor (TIR)-domains that drive the assembly of signalling complexes downstream TLR are present in these organisms. Here, we undertook the characterisation of the repertoire of TIR-domain containing prote...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Tsoumtsa LL,Sougoufara S,Torre C,Lemichez E,Pontarotti P,Ghigo E

    更新日期:2018-09-01 00:00:00

  • Receptors for complement on echinoid phagocytes. II. Purified human complement mediates echinoid phagocytosis.

    abstract::The ingestion of sheep erythrocytes (E) coated with specific IgM antibody (EA) and various purified human complement components (EAC 1, EAC 14, EAC 142, EAC 1423b, EAC 43b) by echinoid phagocytes was examined. E, EA, EAC 1, EAC 14 and EAC 142 were ingested at a similar and very low level, while a strong opsonic effect...

    journal_title:Developmental and comparative immunology

    pub_type: 杂志文章


    authors: Bertheussen K

    更新日期:1982-10-01 00:00:00