Abstract:
:Receptors for the Fc region of IgG (FcγRs) play a key role in protecting the immune system and host from infection. In this study, we described the cloning, sequencing and characterization of porcine FcγRI, and reported six different FcγRI isoforms, four of which have never been reported before. Further analysis revealed that FcγR isoforms are generated by alternative splicing mechanisms, including two membrane isoforms and four soluble isoforms. Importantly, we found FcγRI splice variants differentially influence PRRSV antibody-dependent enhancement (ADE) effects. Membrane pCD64-T1 promotes endocytosis of the PRRSV-antibody complex to enhance PRRSV replication, and soluble pCD64-T3 has no ADE effect on PRRSV proliferation, but shows an inflammation enhancement effect. The differential expression of selective splicing in primary PAM cells and 3D4/21 cell lines are altered and regulated by PRRSV infection and inflammatory environment. Our results indicated that porcine FcγRI plays dual regulatory roles in PRRSV multiplication and PRRSV inflammation process by the alternatively spliced mechanism, which will be a new target in PRRSV prevention and control.
journal_name
Dev Comp Immunoljournal_title
Developmental and comparative immunologyauthors
Shi P,Su Y,Li Y,Zhang L,Lu D,Li R,Zhang L,Huang Jdoi
10.1016/j.dci.2018.09.019subject
Has Abstractpub_date
2019-01-01 00:00:00pages
186-198eissn
0145-305Xissn
1879-0089pii
S0145-305X(18)30442-7journal_volume
90pub_type
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journal_title:Developmental and comparative immunology
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journal_title:Developmental and comparative immunology
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pub_type: 杂志文章,评审
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journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/0145-305x(91)90018-t
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journal_title:Developmental and comparative immunology
pub_type: 杂志文章
doi:10.1016/j.dci.2013.11.012
更新日期:2014-05-01 00:00:00
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journal_title:Developmental and comparative immunology
pub_type: 杂志文章,评审
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更新日期:2013-01-01 00:00:00