当前位置： SCI文献检索 > DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY期刊下所有文献 > Molecular characterization, phylogeny and expression of a hepcidin gene in the blotched snakehead Channa maculata.

Molecular characterization, phylogeny and expression of a hepcidin gene in the blotched snakehead Channa maculata.

Abstract：

:A hepcidin-like gene (cmHep) was cloned and characterized from the liver of the blotched snakehead Channa maculata. The complete cmHep cDNA was 756 bp in length, containing an open reading frame of 270 bp (encoding 89 amino acids), flanked by 210 bp and 276 bp of 5' and 3' untranslated regions, respectively. The deduced peptide of 89 amino acids consisted of 24 aa, 40 aa and 25 aa for signal peptide, prodomain and mature peptide, respectively. The mature peptide had eight cysteines at the identical conserved positions in common with most of other known hepcidins in vertebrates. cmHepc gene displayed a tripartite structure (three exons interrupted by two introns), which organisation was conserved between the blotched snakehead and other fish species. Phylogenetic analysis of hepcidins from C. maculata and other vertebrates showed that major phylogenetic grouping of fish hepcidin coincided with the current euteleosts classification, indicating the multiphyletic evolution of hepcidin in the teleosts. In the Acanthopterygii subclade, there were two distinct additional subclades named as HAMP-Ac1 and HAMP-Ac2. The blotched snakehead hepcidin was in the group HAMP-Ac1, which has the hypothetical iron regulatory sequence [Q-S/I-H-L/I-S/A] motif in N-terminal of mature peptide. The RT-PCR showed cmHep mRNA transcripts were widely distributed in all tissues tested in the blotched snakehead including the liver, gill, intestine, spleen, head kidney and peripheral white blood cell. The most abundant of cmHep mRNA was detected in liver. A significant up-regulation of cmHep expression was detected only in head kidney at 24h post-challenge with Vibrio parahaemolyticus in blotched snakehead adults, no significant differences found in liver, gill, intestine and spleen. The cmHep expression was up-regulated in spleen, head kidney and intestine at 24h post-injection with LPS in blotched snakehead juveniles, liver cmHep expression was not altered. Iron overloading and poly I:C stimulation down-regulated cmHep expression in liver, but did not significantly change cmHep expression in spleen, head kidney and intestine in blotched snakehead juveniles.

journal_name

Dev Comp Immunol

journal_title

Developmental and comparative immunology

authors

Gong LC,Wang H,Deng L

doi

10.1016/j.dci.2013.11.007

subject

Has Abstract

pub_date

2014-05-01 00:00:00

pages

1-11

issue

eissn

0145-305X

issn

1879-0089

pii

S0145-305X(13)00328-5

journal_volume

pub_type

杂志文章

在线工具

Molecular characterization, phylogeny and expression of a hepcidin gene in the blotched snakehead Channa maculata.