当前位置： SCI文献检索 > DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY期刊下所有文献 > Characterization of crustins from the hemocytes of the spider crab, Hyas araneus, and the red king crab, Paralithodes camtschaticus.

Characterization of crustins from the hemocytes of the spider crab, Hyas araneus, and the red king crab, Paralithodes camtschaticus.

Abstract：

:Crustins are distributed across the decapods and are believed to play a significant part in the humoral defense system of their host. In this study, two crustin isoforms from Hyas araneus hemocytes were purified and tested for antimicrobial activity against selected microorganisms. They show both antibacterial and antifungal activity, with highest activity against the Gram-positive bacteria Corynebacterium glutamicum. Sequencing of the transcripts showed them to have a mature peptide of 90 amino acids and differing in three positions in the mature peptide. They were named CruHa1 and CruHa2. Real-time RT-PCR revealed that they mainly are expressed in hemocytes. Screening a cDNA library detected a crustin sequence in Paralithodes camtschaticus hemocytes, coding for a mature peptide of 98 amino acids. It was named CruPc. Based on phylogenetic inference and primary structure, CruHa1 and CruHa2 were placed within the Type I group of crustins, while CruPc belongs to the Type II.

journal_name

Dev Comp Immunol

journal_title

Developmental and comparative immunology

authors

Sperstad SV,Haug T,Paulsen V,Rode TM,Strandskog G,Solem ST,Styrvold OB,Stensvåg K

doi

10.1016/j.dci.2008.10.010

subject

Has Abstract

pub_date

2009-04-01 00:00:00

pages

583-91

issue

4

eissn

0145-305X

issn

1879-0089

pii

S0145-305X(08)00248-6

journal_volume

33

pub_type

杂志文章

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